Labshake search
Citations for Stemcell Technologies :
1051 - 1100 of 1162 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Primary human NK cells were isolated from PBMCs from three donors by negative selection using the EasySepTM Human NK Cell Isolation Kit (STEMCELL Technologies 17955), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 100,000-200,000 CD4+ T cells per well were plated in 96-well V-bottom plates and rested for 1.5 hours at 37 °C ...
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bioRxiv - Immunology 2024Quote: Primary human CD8+ T lymphocytes were isolated from buffy coats of anonymous healthy donors and purified (>95% purity) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (STEMCELL Technologies). Buffy coats were collected at the Siena University Hospital after obtaining written informed consent from each donor and processed following standard ethical procedures outlined in the Declaration of Helsinki ...
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bioRxiv - Immunology 2024Quote: Culture supernatants were prepared by growing 5×107 B cells purified from peripheral blood of either healthy donors or CLL patients by negative selection using RosetteSep™ Human B-cell enrichment Cocktail (#15064; STEMCELL Technologies), in 15 ml RPMI-1640 medium (#8758 ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed and CD4+ T cells were isolated by immunomagnetic negative selection (EasySep™ Human CD4+ T Cell Enrichment Kit, StemCell Technologies). 250,000-500,000 CD4+ T cells per well were plated in 96-well V-bottom plates ...
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bioRxiv - Genetics 2024Quote: ... line Kolf2_c1 line (Wellcome Sanger Institute’s Human Induced Pluripotent Stem Cell Initiative) was cultured in TeSR-E8 complete culture media (Stem Cell Technologies #05991) (37°C ...
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bioRxiv - Immunology 2024Quote: ... Remaining unstained cells was restimulated with 12.5 uL/mL Immunocult Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, catalog no. 10970). Forty-eight hours post-restimulation ...
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bioRxiv - Immunology 2024Quote: Primary monocytes were isolated via negative selection using the EasySep™ Human Monocyte Isolation Kit (Easy Sep, 1x10^9) (Stem Cell Technologies) according to the manufacturer’s protocols from PBMCs collected from healthy donors ...
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bioRxiv - Neuroscience 2024Quote: ... Effector natural killer (NK) cells were isolated from human peripheral blood using Rosette Sep NK isolation kit (Stem Cell Technologies, Vancouver, Canada). NK cells were stimulated overnight with IL-21 (20 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... 10 x 106 PBMCs were enriched for B cells using CD3 T cell depletion via the EasySep™ Human CD3 Positive Selection Kit II (StemCell Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Immunology 2024Quote: ... cones obtained from the Oklahoma Blood Institute (Oklahoma City, OK) using negative selection with the EasySep™ Human T Cell Isolation Kit (cat. no. 17951; STEMCELL Technologies). Alternatively ...
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bioRxiv - Immunology 2024Quote: ... Mario-CAR T cells were selected using the EasySep™ Human PE Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17654) with PE NGFR antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... the mice were irradiated (350 rads) and injected intravenously with CD3+ T cell-depleted cord blood (EasySep Human CD3 Positive Selection Kit, 18051, Stem Cell Technologies) containing approximately 105 human CD34+ hematopoietic stem cells (HSC ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were enriched from peripheral blood of HLA-A2− subjects using the Rosettesep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies) and CD25+ cells were separated using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... CD3+ Tresponder cells (previously enriched from an HLA-A3+ individual using the EasySep™ Human CD3 Positive Selection Kit II; STEMCELL Technologies) were labelled with CPD-eFluor™450 and added to the co-cultures at a 5:1 Tresponder:DC ratio ...
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bioRxiv - Immunology 2024Quote: ... B-cells were then purified from the other harvested PBMCs by negative selection using a Human B-cell Isolation Kit (Stem Cell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: Fresh human intestinal samples were collected and kept in DMEM/F-12 with 15 mM HEPES (DMEM/F-12, STEMCELL Technologies, 36254) and 1% w/v Bovine Serum Albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... BT-002-010) once a week and 7ul/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every other week ...
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bioRxiv - Biochemistry 2024Quote: ... Monocytes were purified from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (# 17858, STEMCELL Technologies, Cologne, Germany) according to the manufacturer’s instructions and seeded in 6-well cell culture plates (1.5 x 106 cells/well ...
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bioRxiv - Developmental Biology 2024Quote: Apheresis cones were flushed using 10 mL of PBS +1% FBS + 2mM EDTA and incubated on a nutator at room temp for 20 min with 500 μL of RosetteSep Human NK Cell Enrichment Cocktail (Stem Cell Technologies). Following the incubation ...
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bioRxiv - Immunology 2024Quote: B cells from tonsils of 5 individuals were enriched using negative selection magnetic beads (EasySep Human B Cell Isolation kit, Stemcell technologies, #17954). They were then pooled to avoid batch effects ...
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bioRxiv - Biophysics 2022Quote: ... Human neutrophils were isolated from whole blood of healthy donors by immunomagnetic negative selection using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies, Vancouver, Canada). Isolation began from an initial blood volume of 0.5-1 ml ...
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bioRxiv - Bioengineering 2021Quote: Primary testicular cells were isolated from human testicular biopsies as previously described.[41] Testicular biopsies were transported to the lab on ice in Hypothermosol® FRS (STEMCELL Technologies, 07935) and processed within 1-2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... T cells were purified from peripheral blood samples by the negative selection EasySepTM Human T cell Enrichment Kit (Catalog #17951, StemCell Technologies, Vancouver, Canada). Enriched T cells were stained with Cell Proliferation Dye eFluorTM 450 (Catalog #65-0842 ...
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bioRxiv - Bioengineering 2021Quote: ... the apical channel medium was removed from the human Lung Airway Chip and the basal medium was replaced with PneumaCult-ALI medium (PneumaCult™-ALI Medium, StemCell Technologies, 05001) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Cancer Biology 2020Quote: Clonogenic assay was realized with cells seeded into 35mm petri dish in semi-solid methylcellulose medium (Methocult™ M3231 for murin cells or Methocult™ H4230 for human cells, Stemcell Technologies). Cells were treated with the drugs for 72 hrs and centrifugated ...
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bioRxiv - Cell Biology 2020Quote: Primary human airway epithelial cells isolated via bronchial brushings from consented healthy or asthmatic subjects were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Bioengineering 2020Quote: ... PBMCs were further purified for CD3+ cells by immunomagnetic negative selection using the EasySep Human T-cell Isolation Kit (STEMCELL Technologies, Cambridge, MA). T cells were frozen at 10–20 × 106 cells per 1 mL of Cryostor CS10 (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were then isolated from the cell layer using the EasySep™ human CD4+ T cell isolation kit (StemCell Technologies #17952), according to manufacturers’ instructions.
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2020Quote: ... Primary T cells were isolated from peripheral blood of healthy donors by negative selection using RosetteSept™ Human T Cell Enrichment Cocktail (STEMCELL Technologies, 15061) and activated with Dynabeads® Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Mice were humanized as we described previously [36] with CD34+ hematopoietic stem cells isolated from human cord blood (CB) using a magnetic activated cell sorting CD34+ progenitor cell isolation kit (Stem Cell Technologies, Belgium). CB was provided by the Cord Blood Bank Central Hospital University (Liège ...
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bioRxiv - Cell Biology 2023Quote: ... assay done by culturing 100-sorted cells LIN-CD34+ CD38- CD9+ or CD9- in to MethoCult™ H4434 with human cytokine (Stem Cell Technologies) and colony formation score at day 12-14 of incubation in 37°C.
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bioRxiv - Cell Biology 2024Quote: ... Isolation was performed within 1 h after the blood draw using the Easy sep human neutrophil negative isolation kit (STEMcell Technologies, Vancouver, Canada) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... quality control tests were routinely performed on hESC cultures, using a human stem cell pluripotency detection qPCR kit (Sciencecell, 0853) and hPSC genetic analysis kit (Stem Cell Technologies, 07550), according to the manufacturer’s protocols.
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bioRxiv - Microbiology 2023Quote: ... Cells were counted and CD19+ B cells were isolated using the EasySep Human Pan-B cell enrichment kit (Cat# 19554, StemCell Technologies, Vancouver, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: T cells were directly isolated from peripheral or cord blood using the RosetteSep Human T-cell Enrichment Cocktail according to the manufacturer’s protocol (Stem Cell Technologies cat#15061). T cells were cryopreserved in 90% fetal bovine serum+10% DMSO and stored in vapor phase liquid nitrogen following isolation ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were immediately processed to isolate CD4+ T cells by negative selection using the EasySep Human CD4+ T Cell Isolation Cocktail (StemCell Technologies, Cat. #17952) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T-cells were isolated via two-stage negative selection with EasySep™ Human Naïve CD4+ T-Cell Isolation Kit II (Stemcell Technologies Cat# 17555); and CD8+ T-cells were isolated by negative selection from PBMCs with Stem Cell Technology Easy Sep CD8+ kit ...
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bioRxiv - Immunology 2023Quote: ... Primary CD8+ and CD4+ T cells were isolated by negative selection through either RosetteSepTM Human CD8+ T Cell Enrichment Cocktail (StemCell technologies, Vancouver, Canada) or RosetteSepTM Human CD4+ T Cell Enrichment Cocktail (StemCell technologies ...
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bioRxiv - Molecular Biology 2023Quote: The human ES cell-lines (WA17 and RUES2; WiCell Research Institute, Madison, WI) were maintained in mTeSR1 Plus medium (STEMCELL Technologies, Vancouver, Canada) on plates coated with Matrigel (BD Biosciences ...
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bioRxiv - Bioengineering 2023Quote: Mononuclear cells from healthy human whole blood and CLL patients were isolated by density centrifugation using SepMate-50 tubes (STEMCELL Technologies, Vancouver, Canada). In short ...
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bioRxiv - Genomics 2023Quote: ... The washed PBMCs were resuspended at 200E6 cells/mL in EasySep buffer and isolated with the EasySep™ Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies, #18063), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: Peripheral blood was collected from Saudi patients into an EDTA-containing blood collection tube and treated with RosetteSepTM Human Progenitor Cell Basic Pre-Enrichment antibody cocktail according to the manufacturer’s instructions (Stem Cell Technologies Catalog#15226). After PBMCs separation and isolation ...