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Citations for Stemcell Technologies :
801 - 850 of 1162 citations for Recombinant Human LDLR protein GST tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Human neutrophils were purified from blood of healthy volunteers with the EasySep neutrophil isolation kit (StemCell Technologies, Vancouver, Canada) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Transduced T cells were depleted of CD56 cells using the EasySep Human CD56 positive selection kit (Stemcell Technologies; 17855) and rested for 24 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cells are cultivated in Epicult basal medium with Supplement C (Epicult-C human media kit, Stem Cell Technologies, Vancouver), 5% FCS ...
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bioRxiv - Neuroscience 2023Quote: Human iPSCs at low passage number were cultured in mTeSR medium (STEMCELL Technologies; Vancouver, BC, Canada; Cat. No. 85850) in 10-cm culture dishes (Thermo-Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... using the EasySep™ Direct Human Neutrophil Isolation Kit according to the manufacturer’s instructions (StemCell Tech, Vancouver, BC, Canada). Each sorting resulted in more than 90% neutrophils ...
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bioRxiv - Immunology 2023Quote: Total monocytes were isolated by negative selection using the EasySep Human Monocyte Enrichment Kit without CD16 Depletion (StemCell Technologies) using the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: FACS purified single cell suspensions (500 cells/per well) were seeded in Human EpiCult-B media (Stem Cell Technologies) supplemented with 5 % FBS on day 0 ...
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bioRxiv - Immunology 2024Quote: ... CD4 and CD8 T cells were isolated separately using EasySep Human CD4+ or CD8+ Enrichment Kits (Stem Cell Technologies). Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... followed by depletion of non-NK lineages using immuno-magnetic beads (EasySep human NK cell enrichment kit, Stemcell Technologies). Samples were sequenced in dedicated flow cells on a HiSeq 2500 (Illumina ...
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bioRxiv - Immunology 2024Quote: ... Human whole-blood samples were diluted with 2% FBS in PBS prior to gradient centrifugation on Lymphoprep (StemCell Technologies). A percentage of the freshly isolated PBMCs was labelled with anti-human CD3 ...
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bioRxiv - Immunology 2024Quote: ... after which bulk T cells T cells were enriched using EasySep Human T Cell Enrichment Kits (Stem Cell Technologies), then maintained in RPMI-1640 (ATCC ...
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bioRxiv - Bioengineering 2024Quote: ... (i) Dextran-coated MNPs and anti-CD4 antibodies (EasySep Human CD4 Positive Selection Kit II, STEMCELL Technologies Canada Inc.) were incubated at a 1:1 ratio over night at 3 – 8 °C ...
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bioRxiv - Cell Biology 2024Quote: ... H1 human embryonic stem cells (WiCell, WA01) were routinely cultured as colonies in mTeSR Plus (STEMCELL Technologies, 100-0276) with 0.2% penicillin/streptomycin (BioConcept ...
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bioRxiv - Immunology 2024Quote: ... T cells were obtained using a CD3+ T cell enrichment kit (EasySep™ Human T Cell enrichment, Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Organoids were expanded fed every other day with complete Intesticult Organoid Growth Medium (OGM, Human, StemCell Technologies, Cat # 06010), supplemented with 10µM Rho Kinase inhibitor Y-27632 (Tocris ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were then enriched from the PBMC samples via the EasySep Human Monocyte Enrichment Kit (StemCell Technologies Inc, #19059). Monocytes were seeded at a concentration of 500.000 cells/mL in blood cell growth media (Sigma-Aldrich ...
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A non-invasive method to sample immune cells in the lower female genital tract using menstrual discsbioRxiv - Immunology 2024Quote: ... CVF was enriched for CD45+ cells using the EasySep™ Release Human CD45 Positive Selection Kit (Stem Cell Technologies) according to manufacturer protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Two million human tumor cells were cultured under mammosphere conditions using 5 mL of complete Mammocult medium (StemCell Technologies) in ultra-low attachment plates (Corning ...
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bioRxiv - Cancer Biology 2024Quote: ... 12 ng/mL human-bFGF (#78006) and 2 ug/mL heparin solution 0.2% (#07980) from Stemcell Technologies (Vancouver, Canada). Neurospheres were dissociated using Accutase (#00-4555-56 ...
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bioRxiv - Cancer Biology 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights EasySep Magnets (StemCell Tech.) according to the manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... NK cell isolation was done by using the negative selection kit “RosetteSep™ Human NK Cell Enrichment Cocktail” (STEMCELL Technologies) according to the company’s protocol ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... and T-cells were isolated via the Easy-Sep™ Rosette Human CD4+ T-cell enrichment kit (Stemcell Technologies, Canada) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2021Quote: ... CD8+ T cells were isolated from PBMCs by positive magnetic selection using EasySep Human CD8 Positive Selection Kit (StemCell Technologies). CD4+ T cells were isolated from PBMCs by negative magnetic selection using EasySep Human CD4 T cell Isolation Kit (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Cancer Biology 2020Quote: ... CD34+ cells were isolated from umbilical cord blood using the EasySep™ Human CD34 Positive Selection Kit II (Stemcell Technologies). Cytapheresis of CML patients were kindly provided by Pr ...
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bioRxiv - Cell Biology 2021Quote: ... co-D12 or –D14 CD34+ cells were isolated using a human CD34 positive selection kit (Stem Cell Technologies, Vancouver, Canada). CD34+ cells (1 × 105/mL ...
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bioRxiv - Immunology 2022Quote: ... Neutrophils were obtained from peripheral blood of healthy voluntary donors using the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies), following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Tissues at 19 w and 20 w were subjected to EasySep™ Human CD45 Depletion Kit II (STEMCELL Technologies, 18259) to remove CD45+ immune cells ...
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bioRxiv - Cancer Biology 2022Quote: ... DNTs were enriched by depleting CD4+ and CD8+ cells using RosetteSep™ human CD4- and CD8-depletion cocktails (Stemcell Technologies). The CD4 and CD8 depleted cells were cultured in 24-well plates pre-coated with 5◻μg/ml anti-CD3 antibody (OKT3 ...
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bioRxiv - Cancer Biology 2020Quote: ... Isolated Lin− breast cancer cells were cultured in Human EpiCult™-B Medium Kit medium (Stem Cell Technologies, Vancouver, Canada) containing a supplement mix ...
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bioRxiv - Microbiology 2022Quote: ... Monocytes were isolated from whole blood by negative selection using RosetteSep human monocyte enrichment cocktail following the manufacturer’s protocol (StemCell Technologies). Using density gradient medium (Lymphoprep ...
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bioRxiv - Synthetic Biology 2020Quote: ... CD4+ and CD8+ T cells were isolated using the RosetteSep Human T cell enrichment cocktail (STEMCELL Technologies, 15022 and 15023), and PBMCs were isolated using Lymphoprep (STEMCELL Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... total CD4+ T cells were isolated using a combination of the human CD4+ T cell RosetteSep™ kit (STEMCELL Technologies) and Ficoll-Paque (Cytiva Inc. ...
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bioRxiv - Immunology 2021Quote: CD14 positive cells were isolated from the PBMCs using the EasySep™ Human CD14 Positive Selection Kit by STEMCELL Technologies™ (Cat# 18058).
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bioRxiv - Cell Biology 2020Quote: For structured illumination microscopy primary NK cells were enriched from peripheral blood of healthy human donors using RosetteSep (Stemcell Technologies). Freshly isolated cells were pre-incubated with anti-CD56 Alexa Fluor 647 (clone HCD56 ...
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bioRxiv - Cancer Biology 2021Quote: ... CD8+ T cells were isolated from freshly-thawed PBMCs using the human CD8+ T cell isolation kit (Stem Cell Technologies). Anti-ILT3 52B8 (1µg/mL ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were isolated from healthy adults using the RosetteSep human CD4+ T cell enrichment cocktail (STEMCELL Technologies, 15062) followed by a density gradient isolation (STEMCELL Technologies ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Monocytes were then purified from isolated PBMCs by negative selection using a Human Monocyte Isolation Kit (Stem Cell Technologies, 19359) and cultured for 7 days in RPMI 1640 media (Gibco ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were used to isolate T cells by CD3-negative selection (EasySep Human T cells Isolation, STEMCELL Technologies, Vancouver, Canada). Tumor-infiltrating lymphocytes (TILs ...
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bioRxiv - Immunology 2021Quote: ... Naive CD4+ T cells were isolated by negative selection with the EasySep Human Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were purified from non-adherent cells using Human Resting CD4+ T cell Isolation Kit (STEMCELL Technologies, 17962). A specific reagent to isolate resting T cells was selected to avoid the presence of pre-activated CD4+ T cells in sequencing samples ...
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bioRxiv - Immunology 2022Quote: ... Intestinal CD4 T cells were isolated by negative selection using the EasySep Human CD4 T Cell Isolation Kit (STEMCELL Technologies). Thymocyte single cell suspensions were obtained by pressing minced tissue though a 70μm strainer ...
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bioRxiv - Cell Biology 2022Quote: hematopoietic progenitors (CD34+CD43+ for iPSC and CD34+ for primary cells) were plated in human methylcellulose medium H4434 (STEMCELL technologies), containing recombinant human cytokines and scored for the presence of colonies 14 days after.
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bioRxiv - Cancer Biology 2022Quote: ... and cultured in a 1:1 ratio of DMEM-F12 and NeuroCult NS-A Basal Medium (Human) (Stem Cell Technologies) with 1X N-2 supplement (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: Human neural progenitor cells (hNPCs) were generated by a single-cell monolayer protocol using STEMDiff Neural Induction Medium (StemCell Technologies) as described previously 19 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were either left unactivated or activated with Immunocult Human CD3/CD28/CD2 T cell activator per manufacturer’s instructions (StemCell Technologies) for 24 or 48hrs ...
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bioRxiv - Immunology 2023Quote: ... France) were processed for the isolation of CD34+ precursor cells using the EasySep Human CD34 Positive Selection Kit (Stemcell Technologies). Isolated cells were cultured for 1-2 weeks in StemSpan Medium (Stemcell Technologies ...
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bioRxiv - Immunology 2023Quote: Monocytes were isolated by depletion of non-monocytic cells using a human monocyte enrichment kit without CD16 depletion (STEMCELL Technologies). PBMC were resuspended at 5ξ107 cells/mL and 50 μL/mL of the non-monocyte cocktail was added ...
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bioRxiv - Cancer Biology 2023Quote: ... CB MNC were enriched for CD34+ cells using the RosetteSepTM Human CB CD34 Pre-Enrichment Cocktail (Stem Cell Technologies, UK) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... HUCB CD34+ cells harvested from Matrigel Matrix were subsequently enriched using EasySep Human Cord Blood CD34 Selection (StemCell Technologies, #17896). Enriched CD34+ were then either used for FACS ...