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Citations for Stemcell Technologies :
1051 - 1100 of 4374 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: Pooled breast epithelial single cells from 15 patients were cultured in MammoCult media (Stem Cell Technologies, Vancouver, Canada) and treated with curcumin ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were harvested from SPLNs and MLNs using a CD4+ T cell isolation kit (STEMCELL technologies). Cells (2 × 105 cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified by negative selection using the EasySep™ CD4 enrichment kit (Stem cell Technologies), and further enriched for memory cells by depletion of naïve T cells using CD45RA beads (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2022Quote: ... Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies) at 1 × 106 cells/mL with 2 μL/mL ImmunoCult™ human CD3/CD28 T cell activator ...
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... The final clear cell pellets (containing mammary epithelial ducts) were dissociated into single cells using Accutase (StemCell Technologies). The obtained single cells were cultured using DMEM/F12 culture medium for IF experiments or used directly for flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from mouse spleens using EasySep mouse B cell isolation kit (Stemcell Technologies, Vancouver, Canada) according to manufacturer’s instruction ...
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Histone H3K36me2-specific methyltransferase ASH1L is required for the MLL-AF9-induced leukemogenesisbioRxiv - Cancer Biology 2021Quote: ... The red blood cells in the bone marrows were lysed by ammonium chloride solution (Stem Cell Technologies 07800) and filtered with a 70-μm nylon filter ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were then washed twice in Ca2+ and Mg2+ free PBS (Stem Cell Technologies, Vancouver, Canada). The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... cultures were initiated at a density of 500,000 cells/mL with serum-free StemSpan SFEM (Stem Cell Technologies) supplemented with Hydrocortisone (1µM) ...
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bioRxiv - Cell Biology 2019Quote: Approximately 1×106 hiPSCs were first detached in cell clumps using the gentle cell dissociation reagent (STEMCELL Technologies) and resuspended in cold PBS containing 50% of Geltrex and kept on ice ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
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bioRxiv - Immunology 2020Quote: ... Naive B cells (CD19+CD27−IgD+) were enriched using Human naive B cell negative selection kit (Stemcell Technologies).
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bioRxiv - Genomics 2021Quote: ... progenitor BM sorted cells were seeded (250 or 500 cells/well) into 6 well SmartDish™ (STEMCELL Technologies). After incubation for 14 days ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... The mouse B cell isolation kit and mouse naïve CD4+ T cell isolation kit were from STEMCELL technologies. The purified rat anti-mouse α4β7 (DATK32 ...
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bioRxiv - Microbiology 2019Quote: ... Pure populations of sorted HPCs were plated either at 500 cells/mL in Methocult H4434 (Stem Cell Technologies) in 6 well plates in triplicate for myeloid colony assays ...
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bioRxiv - Cancer Biology 2021Quote: ... T-cell cultures were fed with fresh 10% RPMI supplemented with human IL2 (20u/ml Stem Cell Technologies) every 2-3 days to maintain 1×106 cells/mL/mm2 ...
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bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell spheroids (1 000 cells/cluster) for transplantation were generated in AggreWell plates (StemCell Technologies, Saint Égrève, France)
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bioRxiv - Immunology 2021Quote: ... Samples were minced and incubated with Stem Cell Spleen dissociation media (Stem Cell Technologies, Vancouver, British Columbia, Canada) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... EasySep human CD4+ T cell enrichment kit and EasySep human CD8+ T cell enrichment kit (STEMCELL Technologies, Canada), respectively ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T cells were obtained by negative selection using the EasySep Human CD4+ Enrichment kit (Stem Cell Technologies), and stimulated with anti-CD3-coated magnetic beads (Dynabeads M-450 tosyl-activated ...
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bioRxiv - Immunology 2021Quote: ... T cells were stimulated with ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stemcell Technologies cat 10990) with 6.25μl per ml culture media at 2e6cells/ml ...
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bioRxiv - Immunology 2020Quote: ... naive CD4+ T cells were isolated using a RoboSep CD4+ Naïve T cell negative selection kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2022Quote: Splenocyte-derived CD4+ T cells were isolated using Easysep Mouse CD4+ T cell isolation kit (19852, STEMCELL Technologies), and then 2e5 cells/well were plated in a 96 well plate ...
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bioRxiv - Immunology 2022Quote: ... eNK cells added to T cell cultures were removed (EasySep Human CD56 Positive Selection Kit II, STEMCELL Technologies) prior to analysis ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were then activated for 3 days in “Complete media” (Immunocult-XF T cell expansion media (STEMCELL Technologies), CTS Immune Cell serum replacement (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD8+ T cell isolation was performed using EasySep Mouse CD8+ T cell Isolation Kit (#19853, STEMCELL Technologies, USA) according to manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies).
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bioRxiv - Immunology 2022Quote: ... T cells were polyclonally activated using ImmunoCult Human CD3/CD2/CD28 T Cell Activator (1:100) (Stemcell Technologies). Before activation and at different time points thereafter ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were enriched by Lymphoprep and RosetteSep Human CD4+ T Cell Enrichment Cocktail (both STEMCELL Technologies). CD4-enriched cells were then CD25-enriched using CD25 MicroBeads II (Miltenyi Biotec) ...
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bioRxiv - Genetics 2022Quote: ... to single cell suspension and 3.6 million cells per well were plated onto Aggrewell400 plates (StemCell Technologies, Canada) (3,000 cells per microwell ...
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bioRxiv - Microbiology 2023Quote: ... cells were differentiated in air liquid interface during 1 month using PneumaCult™-ALI Medium (Stem cell technologies) supplemented with Hydrocortisone stock solution (5 μl/ml ...
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bioRxiv - Cancer Biology 2022Quote: Hypoxia treatment of cells: Plated cells were placed in the interior of a hypoxia incubator chamber (STEMCELL technologies) and flushed with 95% Nitrogen (purity ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were then isolated using a magnet based CD8 T cell isolation kit (19053, StemCell Technologies) and used immediately for experiments ...
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bioRxiv - Immunology 2023Quote: ... Naïve CD4+ T cells were isolated with EasySep Mouse Naïve CD4+ T Cell Isolation Kit (STEMCELL Technologies Inc) according to manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... naive Treg cells were purified by using EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies). For human cell culture ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4+ T cells were isolated by negative selection using the EasySep CD4 T cell isolation kit (Stemcell Technologies) and frozen as dry pellet at −80°C.
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were isolated from PBMCs with the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies). Genomic DNA was isolated from a median of 3.65 million CD4+ T cells (IQR 2.48-4.98 ...
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bioRxiv - Immunology 2023Quote: ... Electroporated cells were transferred to cRPMI medium without antibiotics containing 10 ng/mL hrM-CSF (Stem Cell Technologies) and rested at 37°C for 1 h before stimulation ...
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bioRxiv - Immunology 2023Quote: ... B cells were purified from TMNCs with the EasySep Human CD19 Positive Selection Kit II (Stem Cell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were cultured on Matrigel-coated dishes using either mTeSR-1 or TeSR-E8 medium (Stem Cell Technologies). The medium was replaced daily until cells get 70-80% confluence ...
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies, Taiwan) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... at 2.5 x 106 cells/mL and ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) at 25μL/mL ...
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bioRxiv - Immunology 2023Quote: ... Samples were minced and incubated with Stem Cell Spleen dissociation media (Stem Cell Technologies, Vancouver, British Columbia, Canada) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... CD3+ T-cell populations were isolated using RosetteSep Human T Cell Enrichment Cocktails (Stemcell Technologies, 15023 and 15022) and EasySep Human CD4 T Cell Iso Kit (Stemcell Technologies ...