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Citations for Stemcell Technologies :
951 - 1000 of 1162 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were incubated at 37°C and 5% CO2 in normoxia and in pre-equilibrated human IVC1 supplemented with 50ng/mL Insulin Growth Factor-1 (IGF1) (78078, STEMCELL Technologies) under mineral oil for 1-4h to allow for recovery ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte/stromal cell cultures were produced from HES3 human embryonic stem cells (hESCs, WiCell) maintained using mTeSR-1 (Stem Cell Technologies). hESCs were seeded at 2×104 cells/cm2 in Matrigel coated flasks and cultured for 4 days using mTeSR-1 and passaged with TrypLE (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived microglia (iMGL) were generated from human iPSC-derived hematopoietic progenitors using the STEMdiff Microglia Differentiation Kit and Microglia Maintenance Kit (STEMCELL Technologies) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... The percentage of CD3+ cells was determined by flow cytometry and T cells were stimulated with Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies) per fabricator instructions in IMDM (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... The PBMC pellet was resuspended in PBS supplemented with 2% FBS and 1 mM EDTA and monocyte isolation was performed using the EasySepTM human monocyte isolation kit (Stemcell Technologies) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC from a previously characterized healthy control (line 2135 or C3 in 110) were cultured in mTeSR1 media (Stemcell Technologies) on vitronectin coated dishes ...
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bioRxiv - Immunology 2023Quote: ... B cell purification was performed according to manufacturer instructions with the Stem Cell EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies). After enrichment ...
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bioRxiv - Immunology 2023Quote: ... Primary CD4+ T cells were isolated from PBMCs using an EasySep Human CD4+ T Cell Isolation Kit (17952, StemCell Technologies, Canada) as per manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ILC were isolated from PBMCs by negative selection using the EasySep™ Human Pan-ILC Enrichment Kit according to manufacturer’s instructions (Stemcell Technologies). ILC were sorted using FACSARIA™IIu (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Human CD34+ cells were seeded at the concentration of 5*105 CD34+ cells/ml in serum-free StemSpan medium (StemCell Technologies) supplemented with penicillin ...
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bioRxiv - Bioengineering 2023Quote: ... ∼80% confluent human induced pluripotent stem cells (hPSCs) were dissociated into single cells with the Gentle Cell Dissociation Reagent (STEMCELL Technologies). Bioink consisting of 1.5mg/mL collagen with 5% Geltrex and high-density hPSCs (4×107 cells/mL ...
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bioRxiv - Bioengineering 2023Quote: ... the cell suspension resulting from the dissociation was enriched in CD45+ cells with an EasySep Release Human CD45 Positive Selection Kit (Stemcell Technologies) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2024Quote: NK cells were isolated from PBMCs of healthy donors by depleting CD3 cells using an EasySep human CD3 positive selection kit II (StemCell Technologies). The negative cell fraction was expanded using irradiated (100 Gy ...
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bioRxiv - Bioengineering 2024Quote: ... CD4+ CD45RO+ memory T cells were then isolated by negative selection using an EasySepTM Human Memory CD4+ T Cell Enrichment Kit (STEMCELL Technologies).
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bioRxiv - Bioengineering 2024Quote: Isolated B cells were plated in ImmunoCult-XF B Cell Base medium supplemented with Human B Cell Expansion Supplement (StemCell Technology), seeding at 2.5 x 105 cells/mL in an appropriate format to provide media at a depth of 2-5 mm ...
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bioRxiv - Immunology 2024Quote: ... Human CD8+ T cells were purified from healthy donor blood samples using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies). For cloning ...
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bioRxiv - Immunology 2024Quote: Monocytes were isolated from healthy human donor buffy coat (Department of Transfusion & Tissue Medicine of the Brno University Hospital) using RosetteSepTM Human Monocyte Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Monocytes were isolated from PBMCs by CD14 positive selection using the EasySep™ Human CD14 Positive Selection Kit (18058; Stemcell Technologies) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... CD14+ monocytes were then isolated from the PBMC fraction using the EasySep™ Human CD14 Positive Selection Kit II (STEMCELL Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... We initiated the organoid development by plating hiPSCs at density 20- 30% in TeSR1 medium on a 6-well plate coated with human embryonic stem cell (hESC)-qualified basement membrane matrix (STEMCELL Technologies). The next day ...
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bioRxiv - Neuroscience 2024Quote: Human induced pluripotent stem cell (hiPSCs) were obtained from WiCell (IMR90-4/WISCi004, WiCell) and cultured in mTeSR+ medium (STEMCELL Technologies) supplemented with 1% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... CD4+ T lymphocytes were isolated from PBMCs by positive selection using magnetic beads (EasySep Human CD4 positive selection kit II, StemCell Technologies) and activated using ImmunoCult human CD3/CD28 T cell activator cocktail (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... Blood monocytes and T cells were enriched and obtained by counterflow centrifugal elutriation using RosetteSep human monocyte and T cell enrichment cocktail (StemCell Technologies), respectively ...
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bioRxiv - Immunology 2024Quote: ... at the final concentration of 0.3×106 cells/ml and stimulated with ImmunoCult™ Human CD2/CD3/CD28 T Cell Activator (Stemcell Technologies). In the next step ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were first enriched for memory B-cells using the EasySep Human Memory B Cell Isolation Kit (Stemcell Technologies, catalog #17864) and sequenced using the 10x Genomics pipeline as described by Hurtado et al.37 Sequencing data were analyzed using Cell Ranger and custom Python scripts.
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bioRxiv - Cancer Biology 2024Quote: Purified HSPCs were plated in 96 well plates containing megakaryocyte differentiation medium (StemSpan SFEM II with human low-density lipoproteins (STEMCELL Technologies) and Megakaryocyte Expansion Supplement (STEMCELL Technologies)) ...
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bioRxiv - Physiology 2024Quote: When human iPSCs reached a confluency of approximately 95% they were passaged with AccutaseTM Cell Dissociation Reagent (Cat #07920, StemCell Technologies) and resuspended as single cells in mTesRTM1 medium with 10 µM Y-27632 (Tocris Bioscience) ...
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bioRxiv - Cell Biology 2024Quote: ... In some experiments we also used an additional commercially available ENR medium (here denoted cENR; IntestiCult Human organoid differentiation medium; #100-0214, STEMCELL Technologies) with 100U/ml penicillin-streptomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then stimulated with 12 μL/mL ImmunoCult™(TM) Human CD3/CD28 T Cell Activator (STEMCELL Technologies, cat. #10971).
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bioRxiv - Microbiology 2024Quote: CD4+ T-cells were isolated from frozen PBMCs from 5 study participants using the EasySep Human CD4+ T-Cell Enrichment Kit (Stemcell Technologies). 2 million CD4+ T-cells were then cultured in 96-well plates in duplicate in 200 μL R10+ media supplemented with 100 U/mL IL-2 ...
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bioRxiv - Bioengineering 2024Quote: Patient-derived glioblastoma stem cell lines SD2 and SD3 19 were transduced with GFP-expressing lentivirus or were genetically engineered with a doxycycline-inducible H2B-GFP reporter 19 and cultured in laminin-coated tissue culture flasks in complete NeuroCult NS-A proliferation medium for human cells (Stemcell Technologies). U87MG cells (ATCC ...
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bioRxiv - Immunology 2024Quote: ... γδ TCR+ cells were enriched by immunomagnetic negative selection using an EasySep Human Gamma/Delta T Cell Isolation Kit (STEMCELL Technologies). After purification ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated from peripheral blood of healthy donors under a University of Wisconsin-Madison IRB-approved protocol (2017-1070) using the RosetteSep Human T Cell Enrichment Cocktail (cat. no. 15021, 15061; STEMCELL Technologies). The sequence for a third generation GD2-CD28-OX40-CD3ζ construct was generously provided by Malcolm Brenner (Baylor College of Medicine) ...
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bioRxiv - Immunology 2024Quote: ... CD3 negative selection was performed utilizing the EasySep™ Human APC Positive Selection Kit II (Stem Cell Technologies, Cat-no. 17661) with CD3 APC antibody (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Human peripheral blood mononuclear cells from healthy donors were thawed from cryostorage in ImmunoCult-XF medium (Stem Cell Technologies # 100-0956) containing 100 U ml−1 IL-2 in the presence of ImmunoCult™ CD3/CD28/CD2 T cell activator (Stem Cell Technologies # 10970 ...
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bioRxiv - Cancer Biology 2024Quote: Patient-derived colorectal cancer organoids were established and cultured in our Pathology Department using IntestiCult™ Organoid Growth Medium (Human) following the manufacturer’s protocol (Stemcell Technologies)18 ...
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bioRxiv - Developmental Biology 2024Quote: ... were then subjected to magnetic bead-based NK cell negative selection using the EasySep Human NK Enrichment Kit (Stemcell Technologies 19055). Selected cells were cryopreserved in aliquots of <2x106 cells/mL freezing medium (10% DMSO in FBS).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 50 μM Rho-associated protein kinase (ROCK) inhibitor Y-27632 (Stem Cell Technologies). The cell suspension was distributed into ultra-low attachment 96-well plates (Corning) ...
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bioRxiv - Cancer Biology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation and CD34+ cells were separated using EasySep human CD34+ selection cocktail (StemCell Technologies, Inc.). CD34+ cells were short-term cultured in IMDM medium (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Immunology 2021Quote: ... CECs were isolated from human bone marrow aspirates or PBMC derived CECs culture using EasySep™ Release Mouse Biotin Positive Selection Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... CD4+ or CD8+ T-Cells were isolated from PBMC using EasySep™ Human CD4+ or CD8+ T-Cell Isolation Kit (STEMCELL Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: Primary NK cells were isolated from peripheral blood lymphocytes (PBLs) of healthy donors using the EasySep™ human NK Cell enrichment kit (STEMCELL Technologies). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... cells were centrifuged at 500 x g for 15 min at room temperature and were resuspended in RoboSep buffer for B cells isolation by negative selection with EasySep™ Human B Cell Isolation Kit (Stemcell Technologies) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... Primary human MSC were sorted for CD317 expression and co-cultured with commercially sourced cryopreserved CD4+ human cord blood T cells (Stem Cell Technologies).
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bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...