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Citations for Stemcell Technologies :
651 - 700 of 1162 citations for PRG2 Protein Human Recombinant His Tag HPLC verified since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Immunology 2023Quote: ... and T cells were purified using the EasySep™ Human T Cell Isolation Kit (StemCell Technologies, 17951). Once purified ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were then treated with 20 uL/mL of Immunocult Human Treg Differentiation Supplement (StemCell Technologies) containing recombinant human TGF-B1 and all-trans retinoic acid ...
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bioRxiv - Cell Biology 2023Quote: ... with the addition of 1 μg/L of human interleukin 2 (IL-2, STEMCELL Technologies Inc., USA).
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bioRxiv - Cell Biology 2023Quote: H9 human embryonic stem cells (hESCs; female, WA09, WiCell) were cultured in mTeSR Plus medium (STEMCELL Technologies) with 1% Penicillin-Streptomycin in 8 mg/mL Matrigel-coated (Corning ...
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bioRxiv - Genomics 2023Quote: Primary CD25-CD4+ effector T cells were isolated from fresh Human Peripheral Blood Leukopaks (STEMCELL Technologies, #70500) from healthy donors ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were isolated from PBMCs by EasySep Human T Cell Isolation Kit (StemCell Technologies, Vancouver, Canada), MACSxpress Whole Blood NK Cell Isolation Kit (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Immunology 2022Quote: ... Monocytes were purified from the PBMC population using the EasySep Human monocyte enrichment kit (STEMCELL Technologies; 19059), plated in 96-well plates and differentiated into microglia (iMDM ...
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bioRxiv - Immunology 2022Quote: ... coated with human type IV placental collagen in submerged PneumaCult-Ex Plus media (StemCell Technologies, Vancouver, Canada). When cells reached confluency ...
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bioRxiv - Cell Biology 2023Quote: Human iPSCs were differentiated for 12 days using the STEMdiff™ Hematopoietic Kit (#05310, STEMCELL Technologies, Inc.) [43] ...
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bioRxiv - Immunology 2023Quote: Neutrophils of healthy blood donors were isolated using EasySep™ Direct Human Neutrophil Isolation Kit (Stemcell Technologies). Neutrophils were stimulated with immune-complexes as described above and supernatants collected after 4 h ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2023Quote: ... The human MLL-AF9-transduced cells were cultured in StemSpan SFEM II medium (#ST-09655, STEMCELL Technologies) containing 1% penicillin– streptomycin together with 10 ng/ml rhSCF (#255-SC ...
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bioRxiv - Synthetic Biology 2024Quote: ... Primary CD8+ T cells were isolated using EasySep Human CD8+ T Cell Enrichment kits (STEMCELL Technologies, 19053) and cultured in RPMI-1640 (ATCC ...
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bioRxiv - Cell Biology 2024Quote: ... and neutrophils were isolated from blood using the EasySepTM direct human neutrophils isolation kit (Stemcell Technologies, 19666). They were then centrifuged at 400 x g for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Epithelial cells were enriched using the EasySep™ Human EpCAM Positive Selection Kit II (Stemcell Technologies, #17846) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Primary human CD3+ T cells were isolated by negative selection from cryopreserved PBMCs (STEMCELL Tech, CAT#70025) using the EasySep Human T-cell Isolation Kit (STEMCELL Tech ...
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bioRxiv - Biochemistry 2024Quote: ... Human B cells were then purified by negative selection and magnetic separation (Stem Cell Technologies Cat# 17954). Purified human B cells were pre-activated with anti-human CD40 (clone HB14 ...
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bioRxiv - Immunology 2024Quote: ... edited tCD19+ cells were enriched using the EasySep™ Human CD19 Positive Selection Kit II (STEMCELL Technologies). All cells were rested overnight by culturing in the presence of 100 IU/mL (Tregs ...
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bioRxiv - Systems Biology 2024Quote: Organoid were then differentiated in complete Intesticult Organoid Differentiation Medium (ODM, Human, StemCell Technologies, Cat # 100-0214) supplemented with 10μM Gamma y-secretase inhibitor (DAPT ...
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... The T cells from these PBMCs were activated using Human CD3/CD28 T Cell Activator (STEMCELL Technologies) and compared to the resting T cells cultured in media ...
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bioRxiv - Genomics 2024Quote: ... CD4+ T cells were then isolated using the EasySep Human CD4+ T cell isolation kit (StemCell Technologies). Cells were either cultured directly in RPMI with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... Total CD8+ T cells were isolated using EasySep Human CD8+ T Cell Isolation Kit (StemCell Technologies, #17953). Screening for HLA-A*02-positive individuals was performed with an anti-human HLA-A2 Antibody (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... which involved magnetic bead separation employing EasySep Direct Human Monocyte Isolation Kit (StemCell Technologies, Vancouver, BC, CAN). This technique ensured the attainment of a highly pure monocyte population with minimal contamination from other cell types ...
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bioRxiv - Cancer Biology 2024Quote: The ALDH enzyme activity in human melanoma cells was measured using the Aldefluor assay kit (StemCell Technologies) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: Human pluripotent stem cells were cultured in the commercially defined pluripotency maintaining media mTeSR1 (STEMCELL Technologies #85850) on Cultrex (R&D Systems)-coated tissue culture plates ...
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bioRxiv - Neuroscience 2024Quote: Human Embryonic Stem cell (hESC) line H9 (WA09; WiCell) was maintained in mTeSR plus media (Stemcell Technologies) in normal culture condition of 37 °C and 5% CO2 ...
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bioRxiv - Biophysics 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were isolated via density gradient centrifugation (Lymphoprep, StemCell Technologies Inc, 07801). PBMC samples were then stained with FC Block (422301) ...
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bioRxiv - Bioengineering 2024Quote: ... Monocytes were separated from whole blood using a Rosette-Sep Human Monocyte Enrichment Cocktail (Stem Cell Technologies) combined with Lymphoprep (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... Polyclonal CD8+ T cells were selected using EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies; 17953). Blood monocytes were isolated through negative magnetic sorting (EasySep Human Monocyte Enrichment Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... GBM110 cell line (BTRL, USA) was cultured in NeuroCult NS-A Basal Medium-Human (Stem Cell Technologies) supplemented with NeuroCult NS-A Proliferation Supplements-Human (Stem Cell Technologies) ...
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bioRxiv - Cell Biology 2024Quote: Human dermal fibroblasts (HDFs) were transfected using self-replicating RNA vector ReproRNA™-OKSGM (StemCell Technologies, Canada) according to the manufacturer’s instruction followed by puromycin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... BMPCs were enriched from bone marrow mononuclear cells using EasySep Human CD138 Positive Selection Kit II (StemCell Technologies) and immediately used for ELISpot or cryopreserved in 10% dimethyl sulfoxide in FBS.
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
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bioRxiv - Immunology 2022Quote: ... CD14+ CD16- monocytes were isolated using magnetic negative selection (EasySep Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, Massachusetts) and differentiated into macrophages by culture in RPMI 1640 (Lonza ...
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bioRxiv - Cancer Biology 2020Quote: Frozen human bone marrow mononuclear cells were thawed and washed twice with Iscove’s Modified Dulbecco’s Medium (StemCell Technologies) containing 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2020Quote: ... Monocytes were isolated from PBMC by negative selection using the EasySep™ Human Monocyte Isolation Kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Cell Biology 2022Quote: ... Both epithelial and stromal cell pellets were then suspended in complete MammoCult human medium (STEMCELL Technologies, Inc., 05620) supplemented with hydrocortisone and heparin as per the manufacturer’s instructions and 1% pen/strep (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... 12 w samples were further subjected to EasySep™ Human EpCAM Positive Selection Kit II (STEMCELL Technologies, 18356) to positively select for epithelial cells ...
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bioRxiv - Immunology 2022Quote: ... and CD4 T cells were isolated by immunomagnetic positive selection (EasySep Human CD4 positive selection kit; Stemcell Technologies). Sex and age for these individuals can be found in the metadata corresponding to the RNA-Seq data available from the Sequence Read Archive under accession number PRJNA832136.
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... CD34+ cell enrichment was performed using EasySep™ Human CD34 Positive Selection Kit II (StemCell Technologies, Cat 17856) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... positive immunomagnetic selection of CD45+ cells were performed with EasySep Human CD45 Depletion Kit II (StemCell Technologies, Canada) according to the manufacturer instructions (post-isolation purity 96-99%) ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...