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Citations for Stemcell Technologies :
901 - 950 of 1526 citations for Tert Butyldimethyl 3 4 4 5 5 Tetramethyl 1 3 2 Dioxaborolan 2 Yl Phenoxy Silane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... FACS sorting was performed on an Influx-2 on pre-enriched CD4+ T cells (enrichment using an EasySep CD4+ T cell isolation kit (StemCell Technologies)) ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Developmental Biology 2022Quote: ... cells were collected by Trypsin and half of the well equivalent was plated in 2 ml of methylcellulose medium (Human enriched H4435, Stemcell Technologies). Cells were incubated in the assay for 14 days and then scored.
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bioRxiv - Cell Biology 2022Quote: ... Cell pellets (containing 2 × 106) were cultured for 21 days in MesenCult™-ACF Chondrogenic Differentiation Kit (STEMCELL TECHNOLOGIES, Inc, Canada) to induce differentiation ...
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bioRxiv - Synthetic Biology 2022Quote: ... Two days post-transfection, the cells were replated in a 6 well (Falcon, 353224) and selected in 2 μg/ml Puromycin (STEMCELL Technologies). Every two days ...
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bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... IL-11 and SCF cultures:2 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Synthetic Biology 2021Quote: ... in borate buffer for primary culture cells or Poly-D-Lysine for cells from an iPSC background before being coated with either 10 µg/ml mouse laminin or 5 µg/ml human 521 Laminin (Stemcell Technologies Australia, Melbourne, Australia) respectively to facilitate cell adhesion.
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bioRxiv - Developmental Biology 2024Quote: ... and the organoid pellet was resuspended in 5-10 mL pre-cooled basal DMEM/F-12 medium containing 15 mM HEPES (Stem Cell Technologies, cat.# 36254) using a 10 mL pipette pre-wetted with 1% (vol/vol ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were kept in a 37°C tissue culture incubator with 5% CO2 and lines were maintained in mTeSR Plus cultured media (Stemcell Technologies Cat# 100-1130). Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL ...
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bioRxiv - Genomics 2021Quote: ... after which cells were replated in E8 containing p160-Rho-associated coiled-coil kinase (ROCK) inhibitor Y-27632 (2 μM; Stemcell Tech, 72307).
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
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bioRxiv - Bioengineering 2022Quote: ... were either coated with 70 µL of 2% agarose in water or PBS or treated for 20 min with 100 µL of anti-adherence rinsing solution (StemCell Technologies 07010). The wells were washed with PBS ...
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bioRxiv - Immunology 2021Quote: Whole blood was collected in heparin containing vacuutubes and 12μL of 0.5M EDTA with 1mL of PBS+2% FBS and 50μL of RBC of EasySep RBC Depletion spheres (EasySep ™ RBC Depletion Reagent: 18170, Stem Cell Technologies) were added ...
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bioRxiv - Genomics 2023Quote: ... liver samples in cohort 2 were reduced of dead cell burden using the EasySep Dead Cell Removal (Annexin V) Kit (STEMCELL Technologies, 17899) to improve cell viability to >70% ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
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bioRxiv - Immunology 2022Quote: ... blood was resuspended in PBS/2%FBS/EDTA and separated using Ficoll-Paque Plus (Cytiva, 17144002) density gradient centrifugation in SepMate50 tubes (StemCell Technologies, 85450), followed by two washes in PBS/2%FBS/EDTA ...
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bioRxiv - Immunology 2024Quote: B cells were isolated from 2 healthy PBMC donors using the EasySep™ Human B-cell Isolation Kit (Catalog #17954) from StemCell Technologies. Cells were then stained with APC anti-IgD-APC ...
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bioRxiv - Bioengineering 2024Quote: ... Fresh gonadal and perirenal adipose tissues were minced with scissors and incubated in collagenase type II (2 mg/mL; StemCell Technologies, CA) and DNase I (20 µg/mL ...
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bioRxiv - Developmental Biology 2021Quote: Cells were plated at 2 days after cell sorting (Day 6 post differentiation) into a methylcellulose-based medium containing hematopoietic cytokines (MethoCult M3434, StemCell Technologies, Cat. 03434) at 6000 cells/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Neuroscience 2024Quote: ... with a tet-on SOX9 and NFIA overexpression vector were grown to 30% confluency and then treated with small molecules SB43152 (STEMCELL Technologies; 2 µM), DMH1 (STEMCELL Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20ng/mL recombinant human IL-2 and 7μL/mL of ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies, 10970) every week.
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bioRxiv - Neuroscience 2022Quote: ... medium was refreshed every 7 days by replacing half of the medium with Brainphys neuronal medium supplemented with 2% NeuroCult SM1 neuronal supplement (Stem cell technologies, Köln, Germany) and 1% pen/strep (BP-complete medium).
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Cancer Biology 2023Quote: Untreated 2153L and 2151R tumors were dissociated in 1 mg/ml Collagenase A for 2 hrs at 37°C and TAMs were separated using EasySep™ Mouse F4/80 Positive Selection Kit (STEMCELL technologies, #100-0659) following manufacture’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: AGMs of E10.5 embryos were dissected in PBS with 7% fetal calf serum (FCS) and penicillin/streptomycin (100 U/mL) and cultured as explants for 2 days in medium consisting of Stemspan (Stem Cell Technologies, cat # 09600), 20% fetal calf serum ...
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bioRxiv - Developmental Biology 2022Quote: ... replated at a density of 1.58 × 105 cells/cm2 in Vitronectin XF-coated 24 well plate into cardiomyocyte media (RPMI, 2% B27 supplement plus insulin [Life Technologies Australia, Cat.#17504001], 10% FBS, and 10 µM Y-27362 ROCK inhibitor [Stem Cell Technologies, Cat.#72308]) and cultured overnight ...
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bioRxiv - Immunology 2020Quote: Whole blood was collected into EDTA tubes and then diluted with an equal volume of phosphate-buffered saline (PBS) + 2% fetal bovine serum (FBS) and layered over Ficoll using SepMate™-50 tubes (STEMCELL Technologies Inc., Vancouver, Canada). Cells were centrifuged at 1200×g for 10 min at room temperature ...
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bioRxiv - Genetics 2023Quote: ... the top 40% of ATTO-550 positive cells were sorted as single-cells into individual wells of two 96-well plates with different combination of CellAdhere Laminin-521 (STEMCELL Technologies, Cat#77004, (2 μg/cm2)) or Vitronectin (0.5 μg/cm2) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μM StemRegenin 1 (StemCell Technologies, Inc.), 10% charcoal stripped FBS (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 μM IWR-1-endo (StemCell Technologies, 72562), 5 μM SB-431542 (StemCell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... NPCM is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 without retinoic acid (Life Technologies) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 µM DMH-1 (STEMCELL Technologies, cat# 73632)13 ...
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bioRxiv - Genomics 2021Quote: ... diluted 1:40 in mTeSR-1 medium (StemCell Technologies). hiPSCs were passaged upon treatment with Accutase (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mg/mL DNase 1 (Stem Cell Technologies). The samples were digested for 1 hour at 37°C with constant low agitation using a thermomixer (Eppendorf) ...
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bioRxiv - Neuroscience 2023Quote: ... Tra-1-60 (#60064, STEMCell Technologies at 1:200), stage-specific embryonic antigen-4 (SSEA-4 ...
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bioRxiv - Molecular Biology 2023Quote: ... NeuroDiff medium is composed of Neurobasal:DMEM/F12 (1:1) supplemented with 1% N2-A (StemCell Technologies), 2% B27 (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... with 1% PS and 1 μg/mL doxycycline (StemCell Technologies 72742). Prior to orthotopic injection of primary PDAC cell lines ...
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bioRxiv - Microbiology 2024Quote: ... a 1:1 mixture of organoid supplement (Stemcell technologies, 100-0191) and Intesicult™ OGM Human Basal Medium (Stemcell technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... EB were transferred in Neural Induction Medium (DMEM/F12 1% Glutamax containing 1% [v/v] N2 [Gibco Cat#17502048], 1% [v/v] MEM-NEAA, 1μg/mL Heparin [Stem Cell Technologies Cat#07980], 1% [v/v] Anti-Anti). Seven days after their initiation ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... in Cortical Neuron Culture Medium 1 (CM!): 1:1 Knockout DMEM/F12: BrainPhys neuronal medium without Phenol-Red (STEMCELL Technologies); B27 supplement ...
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bioRxiv - Cell Biology 2020Quote: ... domes diluted 1:1 with advanced DMEM/F12 media (Stemcell Technologies, UK), with 50 μl domes per well ...
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bioRxiv - Physiology 2022Quote: ... then medium changed to 1:1 cFAD+RI:Pneumacult ALI (Stemcell Technologies, UK) for 2 days to ensure monolayers were 100% confluent ...
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bioRxiv - Neuroscience 2024Quote: ... IgG1 κ Isotype (1:188, STEMCELL Technologies, 60070AD.1, 0.2 ug/µL) was used ...
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bioRxiv - Cell Biology 2021Quote: Hepatostellate organoids at day 17 were treated with dissociation buffer, (1:1:1 mixture of 0.25% Trypsin-EDTA, Accutase (Stem cell Tech.), and Collagenase IV (1 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...