Labshake search
Citations for Stemcell Technologies :
751 - 800 of 1526 citations for Tert Butyldimethyl 3 4 4 5 5 Tetramethyl 1 3 2 Dioxaborolan 2 Yl Phenoxy Silane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
-
bioRxiv - Developmental Biology 2024Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemical compounds were prepared and used according to the manufacturers’ guidelines ...
-
bioRxiv - Cancer Biology 2024Quote: ... Two million human tumor cells were cultured under mammosphere conditions using 5 mL of complete Mammocult medium (StemCell Technologies) in ultra-low attachment plates (Corning ...
-
bioRxiv - Genomics 2024Quote: ... Organoids followed by incubation for 5 minutes at 37°C with resuspension with Accutase (Stemcell Technology, Cat No.07922) were dissociated into 10-15 cell clusters by pipetting 10-20 times ...
-
bioRxiv - Developmental Biology 2024Quote: RUES2 hESCs were cultured in a humidified incubator at 37°C and 5% CO2 in mTeSR1 (StemCell Technologies, 85850) on growth factor-reduced Matrigel-coated (Corning ...
-
bioRxiv - Developmental Biology 2022Quote: ... the cells were dissociated and plated at a density of 3×104 cells/cm in STEMdiff™ Neuron Maturation Kit (Stem Cell Technologies, #08510) medium on a double layer of poly-L-ornithine (Sigma-Aldrich #P3655 ...
-
bioRxiv - Bioengineering 2023Quote: ... the media was fully replaced with 2 mL Hematopoietic Media B (STEMdiff Hematopoietic Basal Media (StemCell Technologies) + STEMdiff Hematopoietic Supplement B (200x ...
-
bioRxiv - Neuroscience 2023Quote: ... the cell pellet from one 60 mm dish was resuspended in 2 mL mFreSR medium (Stemcell Technologies) to freeze two vials of the cells by putting them in an isopropanol freezing container at –80°C overnight before transferring them into liquid nitrogen.
-
bioRxiv - Cell Biology 2023Quote: ... Cells were expanded by culturing in RPMI 1640 with B27 with 2 μM of CHIR99021 (Stemcell Technologies). Media was replaced daily for 6-7 days or until cells reached confluency ...
-
bioRxiv - Neuroscience 2024Quote: ... and Lancaster and Knoblich [2] using the commercially available STEMdiff™ Cerebral Organoid Kit from StemCell Technologies. Organoids were generated according to the guidelines from the manufacturer with a few modifications enabling large-scale culturing of appropriate organoid numbers (Figure S1) ...
-
bioRxiv - Developmental Biology 2024Quote: ... The EBs were then incubated in 2 mL Gentle Cell dissociation reagent (Stem Cell Technologies, #100-0485) in a 37°C water bath for 15 minutes followed by vigorous trituration until they formed a single cell solution ...
-
bioRxiv - Cancer Biology 2024Quote: ... T cells were cryopreserved at 2 × 107 cells per ml in CryoStor CS10 cryopreservation medium (StemCell Technologies) until use ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were plated on uncoated low-adhesion 96-well plates in a two-fold dilution series spanning from 2000 down to 4 cells per well in NeuroCult NS-A media (StemCell Technologies, #05751) containing doxycycline at 2 μg/mL ...
-
bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
-
bioRxiv - Cell Biology 2021Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4 °C with a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) 10 μg/mL in PBS ...
-
bioRxiv - Cancer Biology 2020Quote: ... 2×10^4 cells from bone marrow of xenotransplanted mice were transferred to 2ml of MethoCult H4034 optimum methylcellulose medium (Stem Cell Technologies) and plated onto two 35mm dishes ...
-
bioRxiv - Cell Biology 2020Quote: ... the imaging chamber was incubated overnight at 4° C with 10 μg/mL of a mouse monoclonal anti-CD3 (clone UCHT1, StemCell Technologies, #60011) in PBS ...
-
bioRxiv - Cell Biology 2020Quote: ... washed with phosphate buffered saline (PBS) and incubated overnight at 4° C with a mouse monoclonal anti-CD3 antibody (clone UCHT1, StemCell Technologies, #60011) or a mouse monoclonal anti-HA antibody (clone 16B12 ...
-
bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
-
bioRxiv - Cancer Biology 2024Quote: ... washed with DMEM (centrifuged at 300 g for 10 mins at 4°C) and further dissociated into single cells using the NeuroCult Chemical Dissociation Kit (STEMCELL Technologies; #05707) in accordance with the manufacturer’s instructions ...
-
Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
-
bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
-
bioRxiv - Cell Biology 2022Quote: ... They were patterned into definitive endoderm using the STEMdiff Definitive Endoderm Kit per manufacturer’s instructions over 5 days (StemCell Technologies). Cells were passaged on day 5 of differentiation using GCDR ...
-
bioRxiv - Cancer Biology 2022Quote: ... freshly dissected prostate tissues were dissociated in DMEM containing 5% FBS and 1x collagenase/hyaluronidase (STEMCELL Technologies, Vancouver, BC, Canada), followed by trypsinization with 0.25% trypsin-EDTA (Gibco ...
-
bioRxiv - Neuroscience 2022Quote: hiPSCs were cultured at 37ºC and 5% CO2 in a humidified incubator (BINDER C150) in mTeSR1 medium (STEMCELL Technologies, #85850) on Matrigel (Corning ...
-
bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
-
bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
-
bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
-
bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
-
bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
-
bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
-
bioRxiv - Developmental Biology 2022Quote: ... IPSC colonies were picked and passaged every 5 to 7 days after transfer to the mTeSR culture system (Stemcell Technologies).
-
bioRxiv - Cancer Biology 2022Quote: ... containing 2% FBS and the tissue was further dissociated by treatment with dispase (5 mg/mL)/DNaseI (0.1 mg/mL) solution (STEMCELL Technologies). The cell suspension was filtered using a 40 µm cell strainer (Falcon ...
-
bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
-
bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
-
bioRxiv - Cell Biology 2024Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
-
bioRxiv - Neuroscience 2024Quote: ... hiNS were supplied with a continuous perfusion of carbogen (95% O2/ 5% CO2) infused BrainPhys Imaging Optimized media (STEMCELL Technologies) and the following intracellular solution was used for microglial patch-clamp recordings ...
-
bioRxiv - Neuroscience 2024Quote: ... Neural rosettes were isolated after 5 days in culture by selection using STEMdiff Neural Rosette Selection Reagent (STEMCell Technologies, 05832). The resulting NPCs were cultured on PLO and laminin-coated plates ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml H-PDGF-BB (Shenandoah #100-16) and 2 μg/ml heparin (Stem Cell Technologies #07980). All cells were grown in a standard humidified cell culture incubator at 37 °C and 5% CO2 ...
-
bioRxiv - Neuroscience 2020Quote: ... and further expanded in Neurocult NSA with proliferation supplement (StemCell Technologies; 10ng/ml rhFGF-2, 20ng/ml rhEGF) and incubated at 37°C ...
-
bioRxiv - Neuroscience 2021Quote: ... PBMCs separation was performed within 2 h of blood withdrawal using SepMate™ tubes (StemCell Technologies, BC, Canada) as per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2023Quote: ... T cells were cryopreserved at 2×107 cells per mL in CryoStor CS10 cryopreservation media (Stem Cell Technologies) until use ...
-
bioRxiv - Immunology 2023Quote: ... was used at 2 µg/mL.11,14 Immunocult-ACF Human B Cell Expansion Supplement (BAC, Stem Cell Technologies) was used at 1:50 dilution per manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... rosette selection was performed by incubating for 2 hours with Neural Rosette Selection Reagent (Stem Cell Technologies, 05832), forcefully dislodging with warm DMEM/F12 ...
-
bioRxiv - Cancer Biology 2023Quote: Viable BM cells (2×104) were seeded in 1.1 mL Mouse Methylcellulose Complete Media (M3434; Stem Cell Technologies) in uncoated 6 well plates (3471 ...
-
bioRxiv - Cancer Biology 2023Quote: Splenic B cells were isolated from 12-month-old female and male mice (n=3-4/group) after 7 weeks of BBN exposure using EasySep Mouse Pan B cell negative isolation kit (Cat# 19844, StemCell technologies, BC, Canada). The purity and viability of enriched B cells was >90% as assessed by anti-B220 and anti-CD19 mAb staining using flow cytometry (Fig ...
-
bioRxiv - Immunology 2023Quote: ... were cultured for 4 days post-split in IntestiCult™ Organoid Growth Medium and then switched IntestiCult™ Organoid Differentiation Medium (STEMCell Technologies) for 72 hours to promote epithelial stem cell differentiation ...
-
bioRxiv - Bioengineering 2023Quote: ... IMR90-4 iPSCs (WiCell, WB65317) between passages p35∼45 were maintained on Matrigel-coated dishes in mTeSR Plus medium (STEMCELL Technologies, 100-0276) and dissociated using ReLeSR (STEMCELL Technologies ...
-
bioRxiv - Neuroscience 2021Quote: ... Suspension was mixed with 250 ul of 50% Iodixanol solution (25 mM KCl, 5 mM MgCl2, 10 mM Tris pH 8.0, 50% Iodixanol (60% stock from STEMCELL Technologies, 7820), 1x protease inhibitor (Roche ...