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Citations for Stemcell Technologies :
901 - 950 of 982 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The detached colonies were then pelleted at 300g(av) and reseeded with mTeSR Plus medium supplemented with 10 µM Rock Inhibitor (Y-27632, STEMCELL Technologies). At passage 10 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
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bioRxiv - Cancer Biology 2023Quote: ... and S7 cells were grown as floating spheres and maintained in NeuroCult NS-A Proliferation medium and 10% proliferation supplement (STEMCELL Technologies, Vancouver ...
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bioRxiv - Bioengineering 2023Quote: ... was prepared by mixing equal volumes of 3mg/mL neutralized collagen with 10% Geltrex and 8×107 cells/mL cell suspension in mTeSR 1(STEMCELL Technologies) containing 10μM ROCK inhibitor Y27632 (Cayman) ...
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bioRxiv - Neuroscience 2024Quote: ... A 10 µM 2X working solution of CalbryteTM AM dye was prepared in sterile BrainPhysTM Imaging Optimized Medium (BPI, STEMCELL Technologies) containing 0.08% pluronic F-127 (#20050 ...
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bioRxiv - Immunology 2024Quote: ... single cell suspensions harvested from cell culture or isolated from spleen or mediastinal lymph nodes were incubated with 10% Rat serum (STEMCELL Technologies), Fixable Viability Dye (ebioscience ...
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bioRxiv - Cell Biology 2024Quote: ... IL-11 and SCF cultures:2 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... IL-3/IL-6 and SCF cultures:3 Equal volume of medium was added to each well to a final concentration of 10% FBS (STEMCELL Technologies), 1% penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL, in PBS StemCell Technologies) at 37°C for 1 hour were used.
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bioRxiv - Neuroscience 2024Quote: ... and seeded on Geltrex-coated plates in STEMdiff Neural Induction Medium supplemented with SMADi and 10 nM Y-27632 (a ROCK inhibitor) (all reagents from STEMCELL Technologies). For further cultivation ...
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bioRxiv - Bioengineering 2024Quote: ... and reseeded as a single cell suspension at a density of 1.5×104 cells/cm in mTeSR™ Plus supplemented with 10 μM Y-27632 RHO/ROCK pathway inhibitor (72304, STEMCELL Technologies). Media was replaced with Y-27632 free mTeSR™ Plus after 24 hours.
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bioRxiv - Molecular Biology 2024Quote: ... NIM is composed of NBM supplemented with small molecules SB431542 (10 µM, an inhibitor of TGFβ pathway) (72232, Stem Cell Technologies) and LDN193189 (0.1 µM ...
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bioRxiv - Immunology 2024Quote: ... Sample volume was brought to 5 mL and test tubes were placed in a magnetic block for 10 min (“The Big Easy” EasySep magnet, cat. no. 18001, STEMCELL Technologies). Tubes were gently rocked to allow F4/80+ ATM to be magnetically pulled towards the wall of the test tubes ...
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bioRxiv - Immunology 2024Quote: The various culture media were enriched with the following supplements: kinase inhibitors targeting the TGFβ pathway, including SB431542 (SB, 10 μM) (STEMCELL technologies) and LY364947 (LY ...
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bioRxiv - Bioengineering 2024Quote: ... Tissues were dissected into small sections and rubbed on a serrated Petri dish containing 10 mL of an enzyme cocktail comprising 0.5 mg/ mL Collagenase Type IV (Stem Cell Technologies), 0.5 mg/ mL Liberase (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... and seeded as single cells (density of 1.35 × 105 cells/cm2) in mTeSR1 medium supplemented with 10 µM Rho-Associated kinase inhibitor (Stem Cell Technologies). The pancreatic differentiation was done following previously described protocols36,38,59 ...
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bioRxiv - Bioengineering 2024Quote: ... media on the PSCs was changed to fresh mTeSR Plus supplemented with 10 µM ROCK inhibitor Y-27632 (Stem Cell Technologies). For the CASQ2-/- mutation ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... the glucose concentration of LN was adjusted to 10 mM and the following supplements were added freshly before use: 20 ng/ml BDNF (Stem Cell Technologies), 20 ng/ml GDNF (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were plated 48 hrs later at very low density (10 cells/cm2) on Laminin-521 with CloneR supplement (Stem Cell Technology) for clonal selection ...
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bioRxiv - Bioengineering 2021Quote: 5 ×10 3 Floating cells collected at different time points (day 8, day 10, and day 12) were grown in 1 mL of cytokine containing MethoCult H4434 medium (StemCell Technologies, Vancouver) at 37° C ...
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bioRxiv - Developmental Biology 2020Quote: ... Cells were passaged in clumps of 8-10 cells and seeded in mTeSR supplemented with the Rho-associated protein kinase inhibitor γ-27632 (STEMCELL Technologies) at 10 µM for the first day to improve survival ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cells were plated into 96 well flat bottom plates in growth medium supplemented with 10 µM of ROCK inhibitor (Stem Cell Technologies). Cells were plated at low density (2,500 cells/well ...
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bioRxiv - Neuroscience 2024Quote: ... Embryoid body media (EBM) was used for days 2 to 11 (Supplementary Table 2) and supplemented with 10 µM SB431542 (Stemcell Technologies, #72234), 0.1 µM LDN193189 (MedChemExpress ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Immunology 2023Quote: ... were then seeded overnight on mIL-1β capture antibody coated-coverslips in cRPMI medium supplemented with 10 ng/mL hrM-CSF (Stem Cell Technologies). 30 min before harvesting Mitotracker DeepRed (MTDR ...
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bioRxiv - Immunology 2023Quote: ... BLaER1 cells were trans-differentiated into Mo for 6-7 days in cRPMI medium containing 10 ng/mL of hrIL-3 (Stem Cell Technologies), 10 ng/mL hrM-CSF (Stem Cell Technologies ...
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bioRxiv - Developmental Biology 2022Quote: ... EBs were embedded in Matrigel and transferred to 10-cm cell-culture dishes coated with anti-adherence rinsing solution (Stemcell technologies, P2443) in 15ml NI ...
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bioRxiv - Cancer Biology 2023Quote: ... Matrigel was allowed to polymerize at 37 °C for 10-15 minutes before mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies cat#06005), containing Y-27632 (10 μM) ...
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Prevention of tau accumulation through inhibition of hnRNP R-dependent axonal Mapt mRNA localizationbioRxiv - Neuroscience 2023Quote: ... Alomone Labs), 10 ng/mL brain-derived neurotrophic factor (167450-02-STD, tebu-bio) and 500 µM dibutyryl-cAMP (Stemcell Technologies, 73886) and motoneurons were maintained for 25 DIV.
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bioRxiv - Cell Biology 2024Quote: ... cells were replated in mTeSR1 supplemented with 1% P/S and 10 μM ROCK inhibitor (Y-27632, STEMCELL Technologies, Vancouver, BC, Canada) for 24 h ...
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bioRxiv - Immunology 2024Quote: ... using a pre-coated serological pipette until the supernatant was clear of intestinal contents and mucus (10-15 times) and incubated with Gentle Cell Dissociation Reagent (STEMCELL Tech, Canada) for 15 min at room temperature (RT ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated at 8 × 104 cells per well on a Matrigel-coated 6-well plate in mTeSR1 supplemented 10 µM Y27632 (STEMCELL Technologies, 72307). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... into the protein coated 96-well plates containing 100 µl intestinal organoid growth media supplemented with 10 µM Y-27 (Stem cell technologies). Growth of colonies were analysed using brightfield and fluorescent images with EVOS microscope ...
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bioRxiv - Developmental Biology 2024Quote: ... 96,000 human pluripotent stem cells were seeded on Geltrex (Thermo Fisher A14133-01)-coated plates in Stemflex with 10 µM ROCK inhibitor (Y27632) and after 24hours the medium was changed to TeSR-E6 (Stemcell Technologies, 05946) with 6μM CHIR99021 (Day0) ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF iPSCs were plated in a T25 flask in the mTeSR1 medium with ROCK Inhibitor Y-27632 (10 µM, Stem cell Technologies, #72304). After 24 hours ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
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bioRxiv - Cell Biology 2021Quote: ... YTS cells were cultured in RPMI media supplemented with 10% fetal bovine serum and NK92 cells were cultured in Myelocult (Stemcell Technologies, catalogue H5100) supplemented with 200 U/ml of IL-2 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Developmental Biology 2022Quote: ... and 105 cell/well were plated as small colonies (10-20 cell/colony) on Vitronectin-coated 12-well plates using mTeSR1 media (STEMCELL Technologies, Catalog# 12604013) supplemented with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured in serum-free conditions in NeuroCult NS-A Proliferation solution with 10% proliferation supplement (STEMCELL Technologies; Cat# 05750 and #05753) supplemented with 10 ng/ml basic fibroblast growth factor and 20 ng/ml human epidermal growth factor.
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bioRxiv - Cancer Biology 2022Quote: H1 human ESCs were dissociated using accutase and plated as single cells in mTeSR™1 medium supplemented with ROCK inhibitor Y-27632 (10 μM, StemCell Technologies, Inc.) on matrigel to a final density of 20,000-30,000 cells per cm2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The epidermis was separated from the dermis by placing the strips in tubes containing 10 ml of 5U/ml Dispase II (Stem Cell Technologies, USA) and incubated at 4°C overnight and at 37°C for 2-3 hours ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Developmental Biology 2022Quote: ... NPC-iPS cells were treated with 10 µM Rho kinase inhibitor Y-27632 overnight before dissociated to single cells with Accutase (Stem Cell Technology 07922). About 10 million cells were electroporated with 10 μg of AAVS1 zinc finger nuclease-encoding plasmid and 30 μg of AAVS1-tdTomato with one electroporation of 250 V ...
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bioRxiv - Cell Biology 2021Quote: ... to disperse into single cells and replated in mTeSR1 supplemented with 1% P/S containing 10 µM Rock Inhibitor (Y-27632, Stem Cell Technologies #72308). A full protocol is available at the Allen Cell Explorer (www.allencell.org/methods-for-cells-in-the-lab SOP ...