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Citations for Stemcell Technologies :
601 - 650 of 982 citations for 10 14 Cadinene 4 5 Diol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
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bioRxiv - Developmental Biology 2020Quote: ... 10ng/ml bFGF and 5 ng/ml BMP4 and plated in ultra-low attachment plates (Stem Cell Technologies). Two days later ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
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bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
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bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... iPSCs were maintained at 37 °C and 5% CO2 in mTeSR or mTeSR plus medium (Stem Cell Technologies) on Geltrex-coated (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Purified human CB CD34+ HSPC were isolated and cultured at 5×105/mL in StemSpan SFEMII (Stemcell Technologies) supplemented with human stem cell factor (150ng/mL) ...
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bioRxiv - Developmental Biology 2020Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent and cryopreserved in mFreSR according to manufacturer’s instructions (STEMCELL Technologies). All lines were expanded and stored at low passage number.
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bioRxiv - Cell Biology 2022Quote: ... M2 polarization was achieved by stimulating MΦ cells with 20 ng/mL of interleukin 4 (IL4; Stemcell Technologies, Cat#78045) and interleukin 13 (IL13 ...
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bioRxiv - Genomics 2021Quote: ... Cells were split every 3 or 4 days with TrypLE Select (Fisher) and seeded at 1.5-4×10e5 in a Matrigel-coated p35 plate containing 5μM Y27632 (Stem Cell Technologies) (mTeSR+Y ...
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bioRxiv - Bioengineering 2023Quote: ... for 4 days to induce iMSCs before the medium was changed to Complete MesenCult™-ACF Plus Medium (STEMCELL Technologies) for an additional 2 days and then the cells were transferred into a new 24-well plate without an MEF feeder layer ...
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bioRxiv - Immunology 2023Quote: Naïve CD4 T cells were isolated from the pooled spleens and LNs of 4 week old Thy1.1 expressing C57BL/6J mice using the EasySep Mouse Naive CD4 T cell Isolation Kit (StemCell Technologies). Isolated cells were stained with eFluor 450 viability dye (eBiosciences ...
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bioRxiv - Bioengineering 2023Quote: ... The samples were then incubated overnight at 4°C with primary antibody (beta-tubulin III 1:500; STEMCELL Technologies 60052) diluted in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Diluted blood was overlaid carefully on SepMate-15 tubes each containing up to 4 mL Lymphoprep (STEMCELL Technologies, catalog #07851). The layered blood was centrifuged at 1,200 × g for 10 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... 1x108 cells/ml were incubated with 20μl/ml EasySepTM mouse Hematopoietic Progenitor Cell Isolation Cocktail (15min, 4°C, StemCell technologies), RapidSpheres (50μl/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Every 2-4 weeks organoids were passaged by mechanical pipetting Matrigel gently using Dispase in DMEM/F12 media (STEMCELL Technologies #) and several washes with PBS until Matrigel was cleared out ...
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bioRxiv - Immunology 2021Quote: ... followed by TGFβ alone or together with IL-15 (TGFβ: 10 ng/ml, STEMCELL Technologies, Cat# ...
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bioRxiv - Microbiology 2020Quote: ... in infection media consisting of IMDM supplemented with 10% BIT9500 serum substitute (Stem Cell Technologies), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2021Quote: HBs at day 10 and fully differentiated HSCs were detached by Accutase (Stem cell tech.) and 0.05% Trypsin-EDTA ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then suspended in basal media containing 10 µM Y-27632 (Stem Cell Technologies) and counted ...
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bioRxiv - Cancer Biology 2022Quote: ... Erythrocytes were lysed by addition of 0.8% NH4Cl and 10 mM EDTA (Stem Cell Technologies). Cells were collected by centrifugation for 5 min at 400 × g and analyzed using a FACScan flow cytometer (BD Biosciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... the chips were coated with 10 mg/mL of Cell-Adhere Laminin-521 (StemCell Technologies) diluted in PBS with calcium and magnesium (PBS++ ...
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bioRxiv - Genomics 2021Quote: ... ESC/iPSCs were plated in iPS-Brew with 10 μM ROCK inhibitor (Stem Cell Technologies) onto Matrigel-coated (1 mg ml-1 ...
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bioRxiv - Immunology 2023Quote: ... or frozen using cryopreservation medium with 10% DMSO CryoStor® CS10 (STEMCELL Technologies Inc., Canada) and stored in liquid nitrogen.
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bioRxiv - Neuroscience 2024Quote: ... A full-media change with StemFlex supplemented with CloneRTM (Stemcell Technologies; 05888; 1:10 dilution) was performed once ...
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bioRxiv - Neuroscience 2024Quote: ... coated 12 well plates in NM supplemented with 10 ug/mL BDNF (StemCell Technologies, #78005). Neurons were observed after 24-72 h ...
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bioRxiv - Genomics 2023Quote: ... and plated in fresh rhESC media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Bioengineering 2023Quote: ... Colonies were then differentiated using 10 μM CHIR-99021(Catalog # 72052, STEMCELL Technologies, Vancouver, Canada) for 24 hours ...
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bioRxiv - Immunology 2023Quote: ... and cultured in IntestiCult™ Organoid Growth Medium with 10 uM Y-27362 (STEMCELL Technologies).
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bioRxiv - Neuroscience 2022Quote: ... in embryoid body media consisting of complete StemFlex supplemented with 10 μM Y27632 (Stemcell Technologies), 50 ng/mL BMP-4 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in mtesR-plus medium with 10 µm Y-27632 (STEMCELL Technologies, Inc.) for 24 h before this was replaced with complete MTesR plus medium.
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bioRxiv - Microbiology 2024Quote: ... containing StemFlex medium supplemented with 10 μM ROCK inhibitor Y-27632 (STEMCELL Technologies, Vancouver, Canada). From days 1 to 5 ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were subsequently washed using HBSS with 10 mM HEPES (Stem Cell Technologies, 37150) and incubated for 20 minutes to allow for de-esterification of the AM esters ...
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bioRxiv - Developmental Biology 2024Quote: ... culture medium is switched to E6 medium containing 10 µM SB431542 (STEMCELL Technologies, cat #72234) and 0.2 µM LDN193189 (STEMCELL Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 x 106 cells were pelleted in a 15 ml conical tube at 280 x g for 6 min at 4 °C and cultured for 3 weeks with the MesenCultTM-ACF chondrogenic differentiation kit (Stemcell Tech). Pellets were fixed with 4% PFA for 1 hour ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Cryopreserved PBMCs were quickly thawed and resuspended at a concentration of 4×106 cells/ml in Eagle's minimal essential medium (EMEM; Stem Cell Technologies; supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: ... Cultures were passaged 1:6 in 6-well plates every 4-7 days using Gentle Cell Dissociation Reagent (GCDR, STEMCELL Technologies). Cells were cryopreserved in mFreSR.
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bioRxiv - Neuroscience 2021Quote: ... were generated from confluent iPSC cultures by dissociating them to single cells and seeding them at a density of ∼4 × 106 cells per well of Aggrewell 800 6-well plates (StemCell Technologies), equating to ∼2200 cells per EB ...
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bioRxiv - Immunology 2021Quote: ... CD34+ umbilical cord and CD34+ hematopoietic stem/progenitor cell (Ethics Committee Salzburg approval 415-E/1776/4-2014) were isolated using density gradient centrifugation (Lymphoprep; Stemcell Technologies). Primary human cells ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were checked daily for differentiation and were passaged every 3-4 days using Gentle dissociation solution (STEMCELL Technologies, cat # 07174). All experiments were performed under the supervision of the Vanderbilt Institutional Human Pluripotent Cell Research Oversight (VIHPCRO ...
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bioRxiv - Neuroscience 2023Quote: hiPSC line (IMR90-4, WiCell) derived from a healthy donor and fully characterized was cultured with mTeSRTM Plus Medium (STEMCELL Technologies) on growth factor reduced matrigel-coated 6-well plates (Corning ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were transferred into TeSR-E7 medium and maintained until the emergence of iPSC colonies (about 4 weeks) which were then manually isolated and propagated in mTeSR medium (StemCell Technologies). Absence of plasmid integration and clearance of Sendai virus were confirmed ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed with basal medium (500g, 4°C) and replated in Matrigel in mouse IntestiCult Organoid Growth Medium (STEMCELL Technologies) supplemented with Y-27632 (10 μM) ...
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bioRxiv - Immunology 2022Quote: HLA-A2+ monocytes were differentiated into moDCs for 7 days with GM-CSF (50 ng/ml) and IL-4 (100 ng/ml; both STEMCELL Technologies); media and cytokines were refreshed every 2–3 days ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...