Labshake search
Citations for Stemcell Technologies :
801 - 850 of 2238 citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Bioengineering 2024Quote: Two days post-electroporation 500 HSPCs were plated in each well of a SmartDish 6-well plate (STEMCELL Technologies, Vancouver, Canada, cat.: 27370) containing MethoCult H4434 Classic (STEMCELL Technologies ...
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bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.
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bioRxiv - Developmental Biology 2024Quote: ... the cells were plated in 6-well plates using mTeSR media supplemented with 10 µM Y-27632 (Stem Cell Technologies, catalog no. 72304) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at a high density of (250kcells/cm2 in 6-well plates) as a single-cell suspension in StemDiff Neural Induction Media with Dual SMAD Inhibition (STEMCELL Technologies cat #08581), supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304) ...
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bioRxiv - Neuroscience 2024Quote: ... The first step of the protocol to enable embryoid body (EB) formation employed the Spin-EBs formation method performed in AggreWellsTM800 microwell culture plates (Stemcell Technologies, Catalog # 34825). Briefly ...
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bioRxiv - Synthetic Biology 2024Quote: ... genome-edited iPSCs were plated at a density of 250 cells per well of a 6-well plate in mTeSR1 medium supplemented with 1x CloneR2 reagent (cat.: 100-0691; STEMCELL Technologies, Vancouver, Canada). After 48h ...
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bioRxiv - Cell Biology 2020Quote: . Primary human CD3+ and CD8+ T cells were isolated from unfractionated PBMCs using the EasySep Human T cell Isolation Kit and Human CD8 T cell isolation kit with RapidSpheres (Stemcell Technologies). Mobilized human primary CD34+ cells from Peripheral Blood (were obtained from Caltag Medsystems ...
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bioRxiv - Bioengineering 2020Quote: ... CD4pos and CD8pos T cells were isolated by negative selection using EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2021Quote: ... followed by enrichment of either effector/memory T cells (Human Memory CD4 T cell Enrichment kit; EasySep) or Treg cells (Human CD4+CD127loCD49d− Treg Enrichment Kit; EasySep) (all from StemCell Technologies).
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells and CD8+ T cells were enriched using the EasySep Human CD4+ T Cell Isolation Kit and EasySep Human CD8+ T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Neuroscience 2023Quote: NPC (iPSC-derived) patterning toward forebrain neuron fate was conducted using a commercially available kit (STEMdiff Forebrain Neuron Differentiation Kit, STEMCELL Technologies). Cells were maintained in forebrain neuron differentiation medium for 6 days before being dissociated into a single-cell solution and plated onto either Poly-L-Lysine- and Laminin-coated glassware/ Geltrex-coated plasticware at varying densities (6 well plate – 5.2x104 cells/cm2 ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells and CD25hi CD4+ T cells were negatively isolated from PBMCs separately by using Easysep human CD4+ T cell isolation kits and EasySep Human CD4+CD127lowCD25+ Regulatory T Cell Isolation Kit (STEMCELL Technologies), respectively ...
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bioRxiv - Immunology 2023Quote: ... the dead cells were first removed with a Dead Cell Removal Kit and then the NK cells were depleted using the EasySep Human CD56 Positive Selection Kit (Stemcell Technologies). The control 221 cells were kept in the same culture conditions but were not exposed to NK cells ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC-derived microglia (iMGL) were generated from human iPSC-derived hematopoietic progenitors using the STEMdiff Microglia Differentiation Kit and Microglia Maintenance Kit (STEMCELL Technologies) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2023Quote: ... neutrophils were purified by an in-house negative selection kit based on a modified mouse-neutrophil positive selection kit EasySep (Stemcell Technologies). The antibodies used for the negative selection were ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells were isolated from the spleens of young (2- to 3-month-old) mice spleen using a magnetic microbeads negative selection kit (EasySep Mouse CD4 T Cell Isolation Kit; STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Cell growth monitoring and analysis of hESCs trilineage differentiation were performed using the Cell Counting Kit-8 (Dojindo) and STEMdiffTM trilineage differentiation kits (Stem Cell Technology), according to the manufacture’s protocols ...
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bioRxiv - Immunology 2019Quote: CD4+ T-cells were isolated from murine splenocytes and lymph node cells using a T-cell negative selection kit (Easy Sep mouse T-cell enrichment kit, Stem Cell Technologies) to a purity >90% as per the manufactureŕs instructions ...
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bioRxiv - Immunology 2019Quote: CD4+ T-cells were isolated from splenocytes and lymph node cells of wild type C57Bl/6 mice (6-8weeks old) using a T-cell negative selection kit (Easy Sep CD4+ T-cell enrichment kit, Stem Cell Technologies) to > 90% purity according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2021Quote: ... c-Kit+ cells were stained and magnetically isolated from the lineage-depleted cell suspension using the EasySep mouse CD117 (c-Kit) positive selection kit (Stem Cell Technologies), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the Negative Selection EasySep CD4+ T cell kit (17951) or RosetteSep Human CD4+ Cell Isolation Kit (15062) from Stemcell Technologies (Vancouver, Canada) according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2022Quote: ... five to seven day old iPSCs cultures were then used to generate cluster of differentiating 43 positive (CD43+) hematopoietic progenitor cells (HPCs) following a 12 day commercially available kit (STEMdiff Hematopoietic Kit; Stemcell Technologies # 05310). IPSCs were cleaned and 1/3 dish was gently dissociated with dispase (Stemcell Technologies # 07923 ...
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bioRxiv - Neuroscience 2021Quote: T lymphocytes were isolated from the spleen of C57BL/6J mice using a negative selection isolation kit (EasySep™ Mouse T Cell Isolation Kit, StemCell Technologies). Cells were plated in a 48 wells plate (Corning ...
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bioRxiv - Bioengineering 2023Quote: ... we initiated the intestinal organoid differentiation on the strips using a commercially available kit STEMdiff™ Intestinal Organoid Kit (05140, STEMCELL Technologies) containing the necessary medium for each differentiation stage ...
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bioRxiv - Cell Biology 2024Quote: iPSCs were differentiated into contracting ventricular cardiomyocytes using a commercially available kit (STEMdiff™ Ventricular Cardiomyocyte Differentiation kit, Cat # 05010, STEMCELL Technologies). iPSCs were passaged into single cells using Accutase (Cat # A1110501 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Rescue of epithelial integrity was induced by threating hiPSCs for 1 day with LPA (StemCell Technologies, ref. 72694, 5 µM). Chemical disruption of epithelial integrity was induced by threating hiPSCs for 1 day with Blebbistatin (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissected prostate tissues were dissociated in DMEM containing 5% FBS and 1x collagenase/hyaluronidase (STEMCELL Technologies, Vancouver, BC, Canada), followed by trypsinization with 0.25% trypsin-EDTA (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
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bioRxiv - Neuroscience 2022Quote: hiPSCs were cultured at 37ºC and 5% CO2 in a humidified incubator (BINDER C150) in mTeSR1 medium (STEMCELL Technologies, #85850) on Matrigel (Corning ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... IPSC colonies were picked and passaged every 5 to 7 days after transfer to the mTeSR culture system (Stemcell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... containing 2% FBS and the tissue was further dissociated by treatment with dispase (5 mg/mL)/DNaseI (0.1 mg/mL) solution (STEMCELL Technologies). The cell suspension was filtered using a 40 µm cell strainer (Falcon ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Cell Biology 2024Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Neuroscience 2024Quote: ... hiNS were supplied with a continuous perfusion of carbogen (95% O2/ 5% CO2) infused BrainPhys Imaging Optimized media (STEMCELL Technologies) and the following intracellular solution was used for microglial patch-clamp recordings ...