Labshake search
Citations for Stemcell Technologies :
651 - 700 of 2238 citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... A 1-5 min centrifugation of the well-plate was performed at 200g and cells in suspension were cultured for one day in mTesR Plus (StemCell Technologies) to allow for aggregation and Embryoid Body (EB ...
-
bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
-
bioRxiv - Cell Biology 2024Quote: ... the cells were resuspended and 50,000-100,000 cells were transferred into fresh matrix-coated plates with mTeSR Plus media containing 10 μM Y-27632 (ROCK inhibitor, STEMCELL Technologies). Fresh media without ROCK inhibitor was fed the next ...
-
bioRxiv - Microbiology 2023Quote: ... A final boost in PBS was administered and 4 days later splenocytes were fused with Sp2/10 Ag14 cells (ATCC CRL-1581) and cultured overnight prior to concurrent cloning and HAT selection on methylcellulose plates (ClonaCell-HY, StemCell Technologies). After 14 days of incubation at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: ... and the cells were then replated on matrigel-coated plates in mTesR plus media at a density of 40,000 cells/cm2 (Stem Cell Technologies). Cells were allowed to proliferate for two days with a daily media change ...
-
bioRxiv - Molecular Biology 2022Quote: ... T cells were seeded at 2 × 106 cells/mL (typically 10×106 cells per well of a 6-well plate) and activated with ImmunoCult T Cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... Current protocol is optimized for 16 bEPSCs and 16 bTSCs per well in a ∼1200 well Aggrewell 400 microwell culture plate (Stemcell technologies) for a total of 19,200 of each cell types per well ...
-
bioRxiv - Neuroscience 2023Quote: ... Six-well plates coated for at least 2h with 10µg/mL of Cell Adhere Laminin 521 (Stem Cell Technology, 200-0117) diluted in DPBS containing calcium and magnesium (Corning ...
-
bioRxiv - Neuroscience 2023Quote: ... NPCs were digested with Accutase and then differentiated onto PLO/laminin coated plates/coverslips with final differentiation media which is BrainPhys media (STEMCELL Technologies) supplemented with Glutamax (Fisher) ...
-
bioRxiv - Neuroscience 2023Quote: ... Neural progenitor cells were then dissociated and replated on poly-l-ornithine/fibronectin/laminin-coated plates and maintained in BrainPhys Basal medium (StemCell Technologies) containing B-27 supplement ...
-
bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270 ...
-
bioRxiv - Physiology 2023Quote: ... Cells were then plated between 450,000 - 750,000 cells per well of a Matrigel coated 12-well tissue culture plate in mTeSR1 PLUS™ (Stem Cell Technologies) supplemented with 10 µM ROCK inhibitor (Y-27632 ...
-
bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
-
bioRxiv - Genomics 2023Quote: ... human iPSCs were plated at cell density of 3.5×105 cells/well on Matrigel coated 12-well-plates using mTeSR™ medium supplemented with 5µM ROCK inhibitor (Y-27632, STEMCELL Technologies) for 24 h ...
-
bioRxiv - Cancer Biology 2023Quote: The six basal- and luminal-like cell lines were disassociated with 0.25% trypsin and added individually to wells of a 6 well Ultra-low attachment plate in 2 mL Mammocult culturing media (STEMCELL Technologies). Accutase (STEMCELL Technologies ...
-
bioRxiv - Bioengineering 2023Quote: ... 2.5 g of Hydrosil silicone components (1:1 wt) (101301; SILADENT) was added into each well of 6-well microwell culture plate (AggreWellTM400; STEMCELL Technologies) followed by centrifugation at 55 x g for 1 min and curing at 60 °C for 1 h ...
-
bioRxiv - Bioengineering 2023Quote: ... we single cell sorted ATTO550 positive cells for monoclonal expansion in 96-well plates containing their corresponding dTMP-supplemented culture media (hiPSC additionally supplemented with 10% Clone R (StemCell Technologies)) ...
-
bioRxiv - Molecular Biology 2023Quote: The colony-forming unit (CFU) assay was performed by seeding 500 cells in six-well plates containing MethoCult Enriched (StemCell Technologies) after 4 days of editing ...
-
bioRxiv - Biophysics 2023Quote: ... hiPSCs were seeded onto Matrigel-coated 6-well tissue culture treated plates and maintained for 4 days with mTeSR1 media (85850, Stemcell Technologies). On day 0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 1.1 mL of methocult-cell suspension was plated into a 6-well plate using 16-gauge blunt-end needles (STEMCELL Technologies). After 14 days ...
-
bioRxiv - Cell Biology 2023Quote: ... For time first division Single cell LIN-CD34+ CD38- CD9+ or CD9- cells sorted in to the round bottomed 96 well plates and culture in SFEM (StemCell Technologies), supplemented with SCF (10ng/ml) ...
-
bioRxiv - Cell Biology 2023Quote: For cell growth assay 100 LIN-CD34+CD38- CD9+ or CD9- cells were sorted and seeded in 96 well plate and culture in StemSpan Serum –Free Expansion Medium (SFEM, StemCell Technologies) supplemented with 100u/ml penicillin (Hyclone ...
-
bioRxiv - Cancer Biology 2024Quote: ... 1.5×105 day 6 BMDM cells were mixed and seeded together with 300 dissociated GC organoids in 100 µl RGF BME domes in 24-well plate wells and incubated for 72 h in 100 µl IntestiCultTM organoid growth medium (STEMCELL Technologies) containing 20% L929 conditioned medium and 20 ng/ml IL4 ...
-
bioRxiv - Microbiology 2024Quote: Dilution ranges of the test and control drugs were prepared in deep well plates in STEMdiff Cardiomyocyte Maintenance Medium (STEMCELL Technologies). Fluvoxamine ...
-
bioRxiv - Cancer Biology 2024Quote: Purified HSPCs were plated in 96 well plates containing megakaryocyte differentiation medium (StemSpan SFEM II with human low-density lipoproteins (STEMCELL Technologies) and Megakaryocyte Expansion Supplement (STEMCELL Technologies)) ...
-
bioRxiv - Neuroscience 2024Quote: ... or at a density of 5.0 x 104 cells/cm2 on Laminin-/Poly-L-Ornithine coated plates in StemDiff Forebrain Neuron Maturation medium (Stem Cell Technologies) supplemented with 1x Pen/Strep ...
-
bioRxiv - Immunology 2024Quote: ... The purified cells (purity determined as mentioned below) were then plated on Animal-Component-Free coated cell culture plates using Endothelial Expansion Media (StemCell Technologies) for expansion ...
-
bioRxiv - Cell Biology 2024Quote: ... For ECM concentration experiments and mechanical actuation experiments on Mechanoculture FX2 plates fibronectin coating (1 µg/mL and 10 µg/mL, in PBS StemCell Technologies) at 37°C for 1 hour were used.
-
bioRxiv - Cell Biology 2019Quote: Whole-brain cerebral organoids were generated with a commercial kit (STEMdiff Cerebral Organoid Kit, STEMCELL Technologies, Catalogue Number 08570), which has been adopted and optimized based on previously published protocol50 ...
-
bioRxiv - Immunology 2020Quote: Total or naïve mouse CD4+ T cells were isolated from the spleen or mesenteric LNs by negative selection with the EasySep Mouse CD4+ T cell enrichment kit or the EasySep Mouse naïve CD4+ T cell enrichment kit according to the manufacturer’s protocol (Stemcell Technologies). In certain cases ...
-
bioRxiv - Immunology 2024Quote: ... monocytes were enriched from cryopreserved PBMCs using EasySep™ Human Monocyte Enrichment Kit without CD16 Depletion Kit (STEMCELL technologies). Enriched monocytes were stimulated with 100ng/ml LPS for 4h at 37 °C before staining ...
-
bioRxiv - Neuroscience 2021Quote: ... at 5% CO2/95% air condition at 37°C and were passaged using ReLeSR™ (Stem Cell Technologies). Karyotype analysis of G-banded metaphase chromosomes has been performed to confirm chromosomal integrity ...
-
bioRxiv - Cancer Biology 2020Quote: ... and passaged by dissociating to single cells (5-minute treatment with 1X Accutase at 37 °C; STEMCELL Technologies) and resuspending at a concentration of 100k-300k cells/mL.
-
bioRxiv - Developmental Biology 2020Quote: ... and pluripotent stem cells were passaged every 5 to 7 days in the ReLeSR medium (Stem Cell Technologies). Pluripotent iPSC and hESC were differentiated into retinal organoids as previously described (9 and Fig S1 ...
-
bioRxiv - Neuroscience 2022Quote: ... 5 min) and plated at a density of 90,000 cells/mL in STEMdiff Neural Induction Medium (StemCell Technologies) with SMADi supplement (StemCell Technologies ...
-
bioRxiv - Neuroscience 2022Quote: ... on Matrigel-coated culture dishes at 37°C and 5% CO2 and passaged using ReLeSR (Stem Cell technologies). CRX-mCHERRY hiPSCs were grown on vitronectin (VTN-N ...
-
bioRxiv - Cell Biology 2022Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... at 37°C for 5 min and resuspended in E8 plus 10 µM Y-27632 (Stem Cell Technologies) and seeded into AggreWell 800 plates (StemCell Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... and kept on Rho-associated protein kinase (ROCK) inhibitor Y-27632 (final concentration 5 μM, StemCell Technologies, #72302) for one day ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were subjected to red cell lysis in 5 ml ammonium chloride lysis buffer solution (Stem Cell Technologies) on ice for 10 minutes ...
-
Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... Cells were plated at a minimum density of 5 × 105 cells/mL in SFEM II medium (StemCell Technologies) with StemSpanTM CD34+ Expansion Supplement (StemCell Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... This was followed by cardiac specification using RPMI B27-medium containing 5 µM IWP-4 (Stem Cell Technologies) for another 3 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... Approximately 1×10^5 cell per ml were plated in triplicate into M3234 incomplete methylcellulose medium (StemCell Technologies) supplemented with 0.1 ng/mL GM-CSF (Peprotech) ...
-
bioRxiv - Cancer Biology 2021Quote: Ten thousand cells of RPMI-2650 and Detroit celllines were seeded in 24-well Ul-tra-Low Attachment Plates (Costar) containing 1 mL of MammoCult basal medium (Stem Cell Technologies) supplied with 10% MammoCult proliferation supplement ...
-
bioRxiv - Cell Biology 2020Quote: ... cells in a 6 well plate were washed with 1X PBS then subjected to 2.5 units/ml of dispase (Stemcell Technologies, Kent, WA). The plate was placed on an orbital shaker to induce mechanical stress at room temperature ...
-
bioRxiv - Developmental Biology 2020Quote: ... Two conventional and established conditions were used as indicated throughout the figures and manuscript: TeSR conditions on Matrigel coated plates (Stem Cell Technologies) or FGF/KSR conditions on MEF substrate ...
-
bioRxiv - Molecular Biology 2022Quote: ... were a gift from the Nik-Zainal lab (University of Cambridge, UK) and cultured on non-tissue culture treated plates (Stem Cell Technologies) pre-coated with 10 μg/mL Vitronectin XF (Stem Cell Technologies ...
-
bioRxiv - Immunology 2022Quote: ... the media was aspirated from the transwell which was then transported to a new 24-well plate and 600 μL of PneumaCult™ALI Maintenance medium (STEMCELL Technologies) was added to the basolateral side ...
-
bioRxiv - Immunology 2022Quote: ... The transwells were then moved to a new 24-well plate wherein each test well contained 600 μL of fresh PneumaCult™-ALI Complete Base Medium (STEMCELL Technologies) for the basolateral side ...
-
bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...