Labshake search
Citations for Stemcell Technologies :
701 - 750 of 4519 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Primary CD4+ T-cells for in-vitro HIV-1 infection were isolated from total blood of healthy individuals using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2023Quote: ... Primary human B cells were isolated from fresh leuko-reduction collar blood by using 750 µL of RosetteSep™ Human B Cell Enrichment Cocktail (Stemcell Technologies) for 10 mL of collar blood ...
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bioRxiv - Molecular Biology 2023Quote: ... T cells were subsequently isolated from the PBMC population by immunomagnetic negative selective with the EasySep Human T Cell Isolation kit (StemCell Technologies, Canada). Isolated T cells with >95% viability and >95% purity were either cryopreserved or directly cultured for subsequent experiments ...
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bioRxiv - Genetics 2023Quote: Peripheral blood mononuclear cells were isolated by standard Ficoll gradient centrifugation and classical monocytes isolated using EasySep™ Human Monocyte Isolation Kit from Stemcell Technologies according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: CD34+ cells were enriched from de novo progenitor cells collected from each respective 3D hydrogel tissue explant culture using the EasySep Human CD34 Selection (StemCell Technologies, #17856). HUCB CD34+ cells were isolated from whole blood using Lymphoprep (StemCell Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Pathology 2023Quote: ... CD4+ T cells were isolated from 40 ml of peripheral blood from HIV-1-infected individuals on ART by immunodensity negative-selection using the RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (STEMCELL Technologies). Cells were plated at a concentration of 1-3×106/ml in RF10 and stimulated with 2 ng/ml PMA for 16 h when indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... Naïve CD8+ T-cells were isolated from the thawed CD14-population on Day 6 using the EasySep Human Naïve CD8+ T Cell Isolation Kit (STEMCELL Technologies Cat. #19258) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: 50 U/mL IL-2 (Amerisource Bergen, #10101641) and stimulated with 6.25 uL/mL ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies, #10990). Following activation and electroporation ...
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bioRxiv - Bioengineering 2024Quote: ... CD3+ primary human T-cells were isolated by negative selection as per manufacturer’s instructions (Cat # 15021, 15061, RosetteSep Human T-cell Enrichment Cocktail, STEMCELL Technologies, Vancouver, Canada). T-cell pellets were resuspended in dilution medium and counted using a hemocytometer with 0.4% Trypan blue viability stain (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). On the following day ...
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bioRxiv - Cancer Biology 2023Quote: MDSCs were generated from healthy human donors by differentiation of CD14+ monocytes isolated using a RosetteSep human monocyte enrichment kit (STEMCELL Technologies). In order to generate MDSCs ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/mL recombinant human FMS-like tyrosine kinase 3 ligand (Flt-3) and 10 ng/mL recombinant human thrombopoietin (TPO; StemCell Technologies). After sublethal conditioning of 9-12 weeks old Gaa−/− mice with 7.5 Gy gamma-irradiation (1.6 Gy/min ...
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bioRxiv - Immunology 2021Quote: Human NK were isolated from discarded Leukopaks from healthy volunteer blood donors using the RosetteSep™ human NK enrichment protocol (StemCell Technologies), followed by Ficoll (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... Human T cells were also isolated from the thymus tissue in humanized SRG rats via immunomagnetic selection using anti-human CD3 antibody (EasySep™ Human CD3 Positive Selection, Catalog # 17951, Stemcell Technologies) and treated without (vehicle ...
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bioRxiv - Immunology 2023Quote: ... Human blood neutrophils from healthy controls were isolated using negative sorting with the EasySep Direct Human Neutrophil Isolation Kit (STEMCELL Technologies 19666), loaded with DCFDA ...
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bioRxiv - Cell Biology 2024Quote: ... The plates were incubated at 37°C for 20 min to allow the domes to solidify after which 750 μl of human intestinal organoid growth media (Human IntestiCult Organoid Growth Medium, hIOGM, StemCell Technologies, 06010) was added along with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Bioengineering 2021Quote: ... After a 24 hr culture out of thaw the PBMCs were activated with ImmunoCult™ human CD3/CD28 T cell activator reagent from STEMCELL Technologies (#10971) for 3 days according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Cell Biology 2020Quote: Primary human airway epithelial cells isolated via bronchial brushings from consented healthy or asthmatic subjects were grown in PneumaCult ExPlus (Stemcell Technologies, Vancouver Canada) under submerged monolayer culture conditions and used in between passage 1 and 4 ...
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bioRxiv - Immunology 2022Quote: Primary human CD8+ T cells were isolated from buffy coats of healthy donors and purified (>95%) by negative selection using the RosetteSep™ Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies, #15063), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... human peripheral blood monocytes were isolated from freshly prepared PBMCs taken from healthy donors using an EasySep™ Human Monocyte Enrichment Kit (negative selection cell; StemCell Technologies, Canada). Cells were incubated in CTL medium supplemented with 100 U/ml Penicillin-Streptomycin ...
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bioRxiv - Immunology 2023Quote: ... CD34+ human hematopoietic stem/progenitor cells (hHCs) were isolated using the EasySep™ Human CD34 positive selection kit according to manufacturer’s instructions (StemCell Technology, Vancouver, Canada). These cells were cultured ex vivo using various protocols ...
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bioRxiv - Bioengineering 2023Quote: Mononuclear cells from healthy human whole blood and CLL patients were isolated by density centrifugation using SepMate-50 tubes (STEMCELL Technologies, Vancouver, Canada). In short ...
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bioRxiv - Molecular Biology 2023Quote: The human ES cell-lines (WA17 and RUES2; WiCell Research Institute, Madison, WI) were maintained in mTeSR1 Plus medium (STEMCELL Technologies, Vancouver, Canada) on plates coated with Matrigel (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
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bioRxiv - Cancer Biology 2019Quote: ... a Human Peripheral Blood Leuko Pak (Stemcell Technologies #70500.1) from an anonymous donor was diluted with an equivalent volume of PBS + 2% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml human recombinant IL-6 (STEMCELL Technologies) and 10 ng/ml mouse recombinant IL-3 (R&D ...
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bioRxiv - Cancer Biology 2022Quote: RosetteSep™ Human Monocyte Enrichment Cocktail (Stemcell Technologies, Canada) was used to purify human monocytes from buffy coats following manufacturer specifications ...
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bioRxiv - Pathology 2022Quote: Human biopsies were placed in PneumaCult medium (Stemcell Technologies) and sent for infection immediately ...
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bioRxiv - Cancer Biology 2022Quote: ... Human HSPCs were expanded in StemSpan SFEM (StemCell Technologies) with 1% streptomycin/penicillin ...
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bioRxiv - Biochemistry 2019Quote: Human peripheral blood plasma (STEMCELL Technologies, cat. no. 70039) and plasma and bone marrow interstitial fluid samples from three pediatric B-ALL patients (B-ALL-1 ...
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bioRxiv - Immunology 2020Quote: ... and the EasySep human monocyte isolation kit (StemCell Technologies) with equivalent results ...
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bioRxiv - Immunology 2023Quote: Frozen human PBMCs from 5 healthy donors (Stemcell Technologies, Cat# 70025.2 ...
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bioRxiv - Microbiology 2023Quote: ... The RosetteSep™ Human Monocyte Enrichment Cocktail (STEMCELL Technologies) was used to purify monocytes from the PBMCs ...
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bioRxiv - Immunology 2023Quote: Human PBMCs were obtained from Stemcell Technologies (Vancouver, BC). Samples were collected from donors and anonymized prior to providing for research purposes ...
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bioRxiv - Cell Biology 2023Quote: ... and 1000 U recombinant human LIF (StemCell Technologies #78055). Medium was replaced daily ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL, STEMCELL Technologies, #78006.2), human recombinant FGF (10 ng/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (2 ng/mL, STEMCELL Technologies, #78006.2), antibiotic-antimycotic (1X ...
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bioRxiv - Cancer Biology 2022Quote: ... human recombinant EGF (20 ng/mL, STEMCELL Technologies, #78006.2), human recombinant FGF (10 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... 1.5 µg/mL anti-human CD32 antibody (STEMCELL Technologies) and 10 µg/mL anti-mouse CD16/CD32 antibody (as needed ...
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bioRxiv - Genomics 2024Quote: Human iPSCs were maintained in mTeSR1 (StemCell Tech, 85850) or mTeSR Plus (StemCell Tech ...
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bioRxiv - Immunology 2021Quote: Assays were performed on purified human classical monocytes isolated using immunomagnetic negative sorting (EasySep Direct Human Monocyte Isolation Kit, StemCell Technologies, Cambridge, MA). As we have previously described 88 ...
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bioRxiv - Bioengineering 2023Quote: ... CD31+ EPCs were purified using a FITC-conjugated human CD31 antibody (Miltenyi, cat# 130-117-390, clone AC128) and the EasySep Human FITC Positive Selection Kit II (Stemcell Technologies, cat# 18558). Purified EPCs were seeded into collagen IV (Sigma-Aldrich ...
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bioRxiv - Genetics 2019Quote: ... CD4+ T cells fraction of the PBMCs was obtained by negative selection using EasySep® Human CD4+ T Cell Enrichment Kit (Cat. no. 19052, StemCell Technologies, Vancouver, Canada), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated T cells were seeded at a density of 1 x 106 cells/mL in RPMI medium supplemented with 10 ng/mL human recombinant interleukin 2 (IL2, Stemcell Technologies, Cat. No. 78145.2), 1µg/ml anti-human CD3 antibody (BioLegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... for direct experimental use or generated in our laboratories from passage-1 human airway epithelial cells (hAEC; obtained from Epithelix SA) using the differentiation protocols and reagents provided by Stemcell Technologies (PneumaCultTM reagent series). In-house generated cultures were derived from three homozygous F508del-CFTR patients ...
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bioRxiv - Immunology 2023Quote: ... CD4+ T cells were purified from donor 2 LRS cone using a Rosettesep human CD4+ enrichment cocktail (Stemcell Technologies, Cambridge, MA; Supplementary Fig. 1B). 5×106 CD4+ T cells from donor 2 were cultured with 5×105 DCreg from donor 1 in X-vivo media for 5 days ...
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bioRxiv - Neuroscience 2023Quote: Flow cytometric analysis was performed on healthy control PBMCs after 48h stimulation with ImmunoCult™ human CD3/CD28/CD2 T cell activator tetrameric antibody complex (Stemcell Technologies cat. 10970) at 20μl/ml ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 ng/ml human recombinant epidermal growth factor (StemCell Technologies), 1% Penicillin/Streptomycin (Gibco ...