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Citations for Stemcell Technologies :
651 - 700 of 4519 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... CD45+ cells were depleted from the same patient sample to obtain CD45-cells for co-culture using the EasySep Human CD45 Depletion Kit from StemCell Technologies Cat# 17898 ...
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bioRxiv - Immunology 2020Quote: ... Resting CD4+ T cells (rCD4s) were isolated from PBMC by magnetic negative selection using the EasySep Human Resting CD4+ T cell Isolation Kit (StemCell Technologies). In some experiments ...
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bioRxiv - Evolutionary Biology 2020Quote: Cerebral organoids with telencephalic identity from human and ape cells were generated as previously (Lancaster et al. 2017) with the following modification: Aggrewell 800 plates (StemCell Technologies) containing 300 microwells ...
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bioRxiv - Immunology 2020Quote: ... The negative fraction remaining after the positive selection of CD14+ cells was used for pan-DC enrichment employing the EasySep human pan-DC enrichment kit (StemCell Technologies). Total pan-DC were resuspended with 20,000 CD14+ cells and sent for sequencing ...
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bioRxiv - Immunology 2021Quote: ... In vitro experiments were performed using human mononuclear cells from peripheral blood or synovial fluid (SF) isolated by centrifugation in density gradient medium (STEMCELL Technologies). Human monocytes were purified by positive selection with Human CD14 microbeads (Miltenyi) ...
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bioRxiv - Genomics 2021Quote: Human ES cells were differentiated using the STEMdiff Definitive Endoderm Kit (TeSR-E8 Optimized) from STEMCELL Technologies (Ramme et al. 2019). Rat ES cells were differentiated following an optimized mouse protocols (Kim et al ...
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bioRxiv - Neuroscience 2022Quote: ... human PDGF-AA (10 ng/ml) and PDGF-BB (10 ng/ml; Shenandoah) and heparin (2 ng/ml; Stem Cell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... The t(8;21)-positive patient primary cells were co-cultured on MSCs feeders and cultivated with SFEM II containing 1X Human Myeloid Expansion Supplement II (StemCell Technologies).
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bioRxiv - Immunology 2022Quote: Human CD3+ T cells were obtained from buffy coats of healthy donors by negative selection using RosetteSep Human T Cell Enrichment Cocktail Kit (STEMCELL Technologies) and stored at -80ºC until use ...
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bioRxiv - Molecular Biology 2023Quote: ... Peripheral Blood Mononuclear Cells (PMBCs) were isolated from the buffy coat of whole blood (20 mL) using RosetteSep human enrichment cocktail (STEMCELL Technologies) and density gradient centrifugation as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: Human iPSC lines were maintained with daily media changes in mTeSR1 or mTeSR-Plus medium (Stem cell Technologies, 85870 or 05825) as adherent cultures on plates coated with Matrigel (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... co-cultures were counted and recultured as 1 x 106 cells/ml in fresh medium containing 20 U/ml recombinant human IL-2 (StemCell Technologies) in the presence of soquelitinib or DMSO for additional 5 days ...
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bioRxiv - Bioengineering 2023Quote: Human CD8+ T cells were purified from buffy coats from healthy donors at the NIH Blood Bank using EasySepTM Human CD8+ T Cell Isolation Kit (StemCell Technologies). Approximately 2×104 CD8+ T cells were plated in each well of a 96-well plate immediately following isolation ...
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bioRxiv - Bioengineering 2023Quote: ... the cell suspension resulting from the dissociation was enriched in CD45+ cells with an EasySep Release Human CD45 Positive Selection Kit (Stemcell Technologies) following the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2024Quote: ... transduced cells were isolated with the EasySep Human CD271 Positive Selection Kit II with EasyEights™ EasySep™ Magnets (StemCell Technologies) according to the manufacturer protocol.
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bioRxiv - Cancer Biology 2024Quote: ... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
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bioRxiv - Biophysics 2024Quote: NK cells were obtained from anonymous buffy coats (Karolinska Hospital, Huddinge, Sweden) by negative selection using EasySep Direct Human NK cell isolation kit (Stemcell Technology) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... human and murine PBMCs underwent pre-activation through culturing in PBMCs culture medium supplemented with ImmunoCultTM Human CD3/CD28 T cell Activator (StemCell Technologies) and suspension in LymphoGrow II medium (Cytogen) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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bioRxiv - Cancer Biology 2022Quote: Purified human neutrophils were extracted from whole blood using the EasySep™ Human Neutrophil Isolation Kit (Stemcell Technologies, Vancouver, BC) and performed in accordance with the manufacturer’s protocol.
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bioRxiv - Bioengineering 2020Quote: ... Primary astrocyte cultures were passaged using Accutase (Stemcell Technologies).
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bioRxiv - Cancer Biology 2021Quote: ... primary tumor sample was minced in Accutase (StemCell Technologies), and dissociated with glass beads on a nutator for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... Human Recombinant IFNγ (10ng/mL, StemCell Technologies 78020) was added to the media for a period of 60 minutes ...
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BCR analysis of single-sorted, putative IgE+ memory B cells in food allergy: an ephemeral existence?bioRxiv - Immunology 2019Quote: ... or anti-human CD32 (FcγRII Blocker, StemCell Technologies) for 15 minutes on ice to block non-specific staining and then incubated with fluorochrome-conjugated antibodies for 30 minutes on ice and protected from light ...
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bioRxiv - Microbiology 2020Quote: ... and RosetteSep Human CD8 Depletion Cocktail (Stemcell Technologies) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... recombinant human IL-12 (Stemcell Technologies, Vancouver, Canada), IL-15 (Miltenyi Biotec ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant EGF (20 ng/mL, human, STEMCELL Technologies) and antibiotic antimycotic solution (100x ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies, and dead PBMCs were prepared by treating them with 70% ethanol for 30 min ...
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bioRxiv - Immunology 2023Quote: ... frozen human and cynomolgus macaques PBMCs (Stemcell Technologies, Cat# 70025.2 and iQ Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant human Noggin (100 ng/ml, Stemcell Technologies), B-27 supplement (ThermoFisher) ...
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Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastomabioRxiv - Cancer Biology 2023Quote: ... recombinant bFGF (20 ng/mL, human, STEMCELL Technologies), recombinant EGF (20 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... EasySep Human CD34+ Positive Selection Kit (Stemcell Technologies) was used to enrich for CD34+ cells to >90% purity.
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bioRxiv - Immunology 2023Quote: ... and EasySep Human Monocyte Isolation Kit (StemCell Technologies). In the coculture experiment ...
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bioRxiv - Cell Biology 2023Quote: Frozen human PBMCs were purchased from STEMCELL Technologies. After thawing by immediately placing them into a 37°C water bath and transferring them into a pre-warmed RPMI1640 with 10% FBS and 1% Penicillin-Streptomycin ...
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bioRxiv - Molecular Biology 2024Quote: ... Human Intesticult™ (StemCell Technologies, Cambridge, MA, USA) with Primocin® (20 µl of a 500X stock ...
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bioRxiv - Cancer Biology 2021Quote: Metastatic PDX tissues that had been frozen down in either FBS containing 10% dimethyl sulfoxide (DMSO) or primary PDX tissues that were frozen in neuronal stem cell medium (NSC medium, Stemcell technologies, Köln, Germany) containing 10% DMSO were thawed by brief incubation at 37°C and were transferred to basal NSC medium ...
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bioRxiv - Cell Biology 2020Quote: ... A fraction of the cells was stained with an anti-human TRA-1-60 antibody conjugated with Alexa Fluor 488 (StemCell Technologies, Canada) and analysed by flow cytometry using a BD FACSCantoII according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc., Canada) and FACS on a BD FACSAriaTM II using the following markers/staining ...
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bioRxiv - Cell Biology 2022Quote: HSPCs were purified from discarded umbilical cord blood samples of healthy male or female newborns using the EasySep Human CD34 Positive Selection Kit II following pre-enrichment using the RosetteSep Pre-enrichment cocktail (Stem Cell Technologies) and mononuclear cell isolation on Ficoll-Paque (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: Fresh bone marrow was collected into EDTA and purified within 12 hours of collection by negative selection using the RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail according to the manufacturer’s protocol (15026, StemCell Technologies, Cambridge, UK), followed by density gradient centrifugations with Ficoll-Paque Premium (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
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bioRxiv - Cancer Biology 2021Quote: ... Isolation of B-cells from healthy donor and MM patients were performed using EasySepTM Human B-Cell Isolation Kit according to manufacturer’s protocol (Cat. No. 17954, Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Cancer Biology 2021Quote: CD138+ myeloma cells were isolated from patient peripheral blood using a magnetic bead-based approach (EasySep Human CD138 Positive Selection Kit, StemCell Technologies 17877). The CD138+-enriched sample was titrated into 2.5e7 ...
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bioRxiv - Neuroscience 2020Quote: ... gene were cultured according to Stem Cell Technologies protocols that can be found in (https://www.stemcell.com/maintenance-of-human-pluripotent-stem-cells-in-mtesr1.html) on feeder free in hESCs medium on Matrigel (StemCell Technologies, Cat. #354277) in mTeSR (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... Primary human CD8+ were isolated from healthy donor blood with commercially available negative selection kits (RosetteSep Human CD8+ T cell Enrichment Cocktail, STEMCELL technologies, UK) following the manufacturers instruction ...
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bioRxiv - Bioengineering 2019Quote: ... EGFP-transfected) cells were cultured on laminin-coated tissue culture flask in NeuroCult™ NS-A proliferation media for human (STEMCELL Technologies). Culture media was changed every two days ...
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bioRxiv - Neuroscience 2019Quote: ... followed immediately by engrafting 1-5×104 human umbilical cord CD34+ stem cells (Lonza Incorporated, Walkersville, MD; or Stemcell Technologies, Vancouver, BC) via intrahepatic injection ...
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bioRxiv - Cancer Biology 2020Quote: ... containing 100 ng/mL recombinant mouse SCF and 100ng/mL recombinant human TPO for a total of 7 days (all from Stem Cell Technologies).
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bioRxiv - Developmental Biology 2021Quote: Culture of isolated human CB-derived CD34+ cells was performed in HSPC expansion media comprised of Stem Span serum-free media (StemCell Technologies #09855) supplemented with Glutamax (Invitrogen #35050061) ...
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bioRxiv - Immunology 2021Quote: ... T cells at a concentration of 2e6/ml were re-stimulated with ImmunoCult™ Human CD3/CD28/CD2 (Stemcell Technologies cat 10970) at 6.25μl/ml ...