Labshake search
Citations for Stemcell Technologies :
601 - 650 of 652 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... 96,000 human pluripotent stem cells were seeded on Geltrex (Thermo Fisher A14133-01)-coated plates in Stemflex with 10 µM ROCK inhibitor (Y27632) and after 24hours the medium was changed to TeSR-E6 (Stemcell Technologies, 05946) with 6μM CHIR99021 (Day0) ...
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bioRxiv - Developmental Biology 2021Quote: ... Scleral punches were initially placed into wells of a 96 well culture plate with 50 μl of N2 medium [Ham’s F-12/DMEM containing 1x N2 supplement (Stem Cell Technologies, Vancouver, BC) until all sclera were obtained ...
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bioRxiv - Developmental Biology 2021Quote: ... and then seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution following instruction (Stem Cell Technologies, #07010). The culture medium was slightly changed without disturbing aggregates in the V-shape bottom every day and aggregates were collected on day 6 ...
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bioRxiv - Bioengineering 2020Quote: ... and plated onto Matrigel®-coated six-well culture plates in mTeSR™1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y27632 (ROCK inhibitor; Stem Cell Technologies) at a density between 25,000 and 50,000 cells cm-2 ...
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bioRxiv - Neuroscience 2021Quote: ... cells were plated on Matrigel (In Vitro Technologies, cat. # FAL354277) coated plates in StemFlex medium supplemented with 10 μM ROCK inhibitor (StemCell Technologies, cat. #. 72304). Three days after plating ...
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bioRxiv - Neuroscience 2021Quote: ... The transfected LCLs were then plated into a matrigel-coated six-well plate and maintained in TeSr-E7 xeno-free containing media (Stem Cell Tech Inc.). Twelve days after transfection ...
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bioRxiv - Developmental Biology 2022Quote: ... and 105 cell/well were plated as small colonies (10-20 cell/colony) on Vitronectin-coated 12-well plates using mTeSR1 media (STEMCELL Technologies, Catalog# 12604013) supplemented with 10 μM ROCK inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2022Quote: ... and plated at 10,000 cells per well in PDL-coated 96-well plates in BrainPhys Neuronal Medium (BrainPhys (STEMCELL Technologies; Cat. No. 05790) as the base ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng/ml BMP4 supplemented with 10 μM Y-27632 in each well of 6-well AggreWell 400 plates (Stem Cell Technology, 34415). Next day the formed cell spheroids clusters were washed with RPMI-1640 medium with 1x glutamax ...
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bioRxiv - Pathology 2020Quote: ... human iPS-GFP IMR-90-4 reporter cells were maintained in an undifferentiated state in mTesr1 media on plates coated with Matrigel and were routinely passaged with Versene (StemCell Technologies, Vancouver, CA) in a ratio of 1:6 ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then seeded in triplicates at a concentration of 100,000 cells/well into 6-well culture plates with M3434 methylcellulose (Methocult, Stem Cell Technologies, Vancouver, Canada). For assessing the differentiation pattern of naïve bone marrow cells and MDPs in the presence of tumor-derived conditioned medium (TCM) ...
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bioRxiv - Genetics 2020Quote: ... Transduced cells were expanded to 10 cm plates and 10 vials (2M cells/vial) of each cell line was cryopreserved in CryoStor® (StemCell Technologies, 07930) for further use.
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bioRxiv - Cancer Biology 2021Quote: ... and then removed from the Parafilm and transferred to wells of a 24-well plate (one form per well) containing 1.5 mL of neural stem cell media (NeuroCultTM; 05700, STEMCELL Technologies, Vancouver, BC, Canada) supplemented with human EGF (20 ng/mL ...
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bioRxiv - Developmental Biology 2023Quote: ... 7.5-9.0×104 cells were mixed with 0.5 mL culture medium and seeded into one well of 24-well aggreWell400 culture plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies, 07010) following the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... spectra were selected and seeded as single cell per well in a Geltrex coated 96-well plate containing E8 completed medium supplemented with Clone-R (STEMCELL Technologies, Vancouver, Canada). Plates were spun down at 400rcf for 1 minute to increase single cell attachment and were expanded at 37 °C in a humified atmosphere in the presence of 5% v/v CO2 until colonies were formed that could be used to generate a culturable cell line.
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bioRxiv - Neuroscience 2024Quote: ... Cells were digested the following day with Accutase and single-cell plated to 96-well plates in TeSR™-E8™ media with 0.25 μM Thiazovivin and CloneR™ supplement (StemCell Technologies) for clonal isolation and expansion as previously described [32] ...
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bioRxiv - Developmental Biology 2024Quote: ... The Matrigel droplets were incubated at 37 °C for 30 mins before they were washed into 6-well ultra-low attachment plate (Stem Cell Technologies, 38071) containing STEMdiff kit Expansion media with 1 µM SB431542 and 1 µM CHIR99021 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were maintained in standard tissue-culture treated 24-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276) and 1x Penn/Strep from a 100x stock (Mediatech ...
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bioRxiv - Neuroscience 2023Quote: ... 3 million iPSCs per cell line were plated into 1 well of an AggreWell™800 24-well plate (Stem Cell Technologies, 34811) with 2 mL STEMdiff™ Neural Induction Medium + SMADi + 10 µM Y-27632 ...
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bioRxiv - Bioengineering 2024Quote: Two days post-electroporation 500 HSPCs were plated in each well of a SmartDish 6-well plate (STEMCELL Technologies, Vancouver, Canada, cat.: 27370) containing MethoCult H4434 Classic (STEMCELL Technologies ...
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bioRxiv - Genetics 2023Quote: ... The remaining positive cells were bulk sorted in a single well of a Vitronectin coated 6-well plate containing Essential 8 medium and ROCK inhibitor (STEMCELL Technologies, Cat#72304) for the purpose of measuring editing efficiency.
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bioRxiv - Developmental Biology 2022Quote: ... and 1 × 106 cells were seeded per well of an Aggrewell™800 plate in APEL2 medium supplemented with 10 μM Y-27632 ROCK inhibitor (#72307, StemCell Technologies Inc.) followed by 72h incubation with daily media change ...
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bioRxiv - Developmental Biology 2024Quote: ... the cells were plated in 6-well plates using mTeSR media supplemented with 10 µM Y-27632 (Stem Cell Technologies, catalog no. 72304) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at a high density of (250kcells/cm2 in 6-well plates) as a single-cell suspension in StemDiff Neural Induction Media with Dual SMAD Inhibition (STEMCELL Technologies cat #08581), supplemented with 10 µM ROCK inhibitor Y-27632 (STEMCELL Technologies cat #72304) ...
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bioRxiv - Developmental Biology 2024Quote: ... iPSCs were maintained in standard tissue-culture treated 6-well plates in stem cell maintenance medium plus supplement (mTeSR+ kit, StemCell Technologies 100-0276). Cells were passaged using Accutase (StemCell Technologies ...
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bioRxiv - Neuroscience 2024Quote: ... The first step of the protocol to enable embryoid body (EB) formation employed the Spin-EBs formation method performed in AggreWellsTM800 microwell culture plates (Stemcell Technologies, Catalog # 34825). Briefly ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: Mouse Intestinal Organoids were incubated in 24-well cell culture plates at 37 °C under 5% CO2 in IntestiCult Complete Organoid Medium (#06005, Stem Cell Technologies, Canada) containing CHIR-99021 (3 μM final concentration ...
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bioRxiv - Bioengineering 2024Quote: ... hiPSCs were seeded on a six-well plate coated with Matrigel (0.5 mg/six-well plate, dissolved in DMEM/F-12 medium) in mTeSR Plus medium (StemCell Technologies, Vancouver, BC, Canada). Once reaching 60% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 mL of cell suspension was added to each well of a 12-well Collagen I-coated plate (STEMCELL Technologies, #100-0363). Cells were allowed to settle overnight in a humidified incubator at 37°C with 5% CO2.
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bioRxiv - Microbiology 2024Quote: ... These cells were cultured on collagen-coated 100-mm dishes or 6 well plates using PneumaCult-Ex Plus medium (#05040; StemCell Technologies, Vancouver, BC).
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bioRxiv - Synthetic Biology 2024Quote: ... genome-edited iPSCs were plated at a density of 250 cells per well of a 6-well plate in mTeSR1 medium supplemented with 1x CloneR2 reagent (cat.: 100-0691; STEMCELL Technologies, Vancouver, Canada). After 48h ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR2 (STEMCELL Technologies, 100-0691) following nucleofection ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10,000 cells were plated per 12-well plate well onto 5% biolaminin 521 LN and differentiation started 6 hrs post plating in TeSR-E6 (Stem Cell Technologies, 05946) with CHIR99021 (Tocris ...
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bioRxiv - Bioengineering 2024Quote: ... the spheroids were harvested into Eppendorf tubes and then transferred to a black 96-well plate pretreated with anti-adherence rinsing solution (Stem Cell Technologies 07010) (∼40 spheroids per well) ...
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bioRxiv - Developmental Biology 2024Quote: ... and cells plated on fibronectin-coated 96-well plates at 20 000 cells / well in Neuro-Cult Proliferation solution (Stem Cell Technologies 05702) with heparin (Stem Cell Technologies 07980) ...
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bioRxiv - Bioengineering 2020Quote: MSCs at passages 1 or 3 were seeded in wells of a 12-well plate containing 3 ml Mesencult (Stem Cell Technologies, Vancouver, B.C.) each at 8.0×104 ...
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bioRxiv - Cell Biology 2021Quote: Primary amniocyte cells were collected from the amnion after trypsination and were cultured on six-well plates coated with bovine collagen type I/III (StemCell Technologies, Saint-Egrève, France) as described previously (Marceau et al ...
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bioRxiv - Molecular Biology 2024Quote: ... The assay measures the number of colonies formed in the myeloid-defined media after two weeks in culture using the stem vision plate analyzer (Stem Cell Vision, Stem Cell Technologies, Vancouver, Canada).
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bioRxiv - Microbiology 2024Quote: ... Monolayer cultures were seeded into 96-well plates using a 1:1 ratio of IntestiCult™ Organoid Growth Medium Human Basal Medium (StemCell Technologies, 100-0190) and Organoid Supplement (StemCell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells are resuspended in N2B27 medium supplemented with ROCK inhibitor and seeded at a density of approximately 75 cells per microwell in 400 µm AggreWell plates (Stem Cell Technology, cat# 34425). Once cell structures appear solid ...
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bioRxiv - Genomics 2024Quote: ... Approximately 45 mg of flash-frozen skin or/fin was placed in a 6-well tissue culture plate (Stem Cell Technologies, cat. no. 38015) with 1 mL of TST buffer composed of 2 mL of 2X ST buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Daily media changes of E7™ Reprogramming Media followed until the emergence of iPSC colonies between day 21-28 when iPSC colonies were picked and transferred to Geltrex™ coated 12-well plates containing 1 mL TeSR™-E8™ (STEMCELL Technologies) supplemented with 10 μM ROCK Inhibitor (Y-27632 ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were dislodged from the AggreWell™ plate by forcefully dispensing DMEM/F12 and applied to a 37 µm cell strainer (Stem Cell Technologies, 27250) to get rid of dead cells.
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bioRxiv - Molecular Biology 2024Quote: ... transferred to a 24-well plate to solidify at 37°C for 30 min and overlaid with 750 μl IntestiCult Organoid Growth Medium (StemCell Technologies, Vancouver, BC, Canada) and maintained at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were resuspended in N2B27 medium supplemented with a ROCK inhibitor and seeded at a density of approximately 75 cells per microwell in 400 μm AggreWell plates (Stem Cell Technology, cat# 34425). Once the cell structures appeared solid ...
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bioRxiv - Bioengineering 2024Quote: ... aggregated seeds were cultured in complete human hepatocyte culture media (HHCM, LifeNet Health®, MED-HHCM) in Aggrewells™ 400 6-well plates (Stemcell Technologies™) for either 4 days or 7 days ...
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bioRxiv - Cell Biology 2024Quote: ... and the Oregon Green positive cells were sorted into 1 cell/96-well in Matrigel-coated 96-well plate with 1x CloneR2 (Stem Cell Technologies, # 100-0691) and 100µL/well StemFlex using BD FASCMelody cell sorter.
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bioRxiv - Cell Biology 2023Quote: ... and the picked colonies were allowed to grow larger and they ‘passaged’ by mechanical splitting into a new Matrigel coated plate containing mTeSR™1 media with its supplement(Anonymous, n.d.) and maintained as suggested by the manufacturer (StemCell Technologies, Canada Cat. No. #85850). Subsequent passages were done by enzymatic dissociation (Accutase® ...
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bioRxiv - Bioengineering 2024Quote: A control line of iPSC cells was purchased from the BCM stem cell core and maintained on Matrigel coated tissue culture plates using mTeSRTM Plus (Stem Cell Technologies, Vancouver, BC, Canada) with media changes every day.