Labshake search
Citations for Stemcell Technologies :
551 - 600 of 652 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... at 37°C for 5 minutes and replated in a ratio of 1:6 or 1:9 on new Matrigel-coated plates in E8 supplemented with 5 μM ROCK inhibitor Y-27632 (Stemcell Technologies, Canada) for 24h ...
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bioRxiv - Developmental Biology 2020Quote: ... Two conventional and established conditions were used as indicated throughout the figures and manuscript: TeSR conditions on Matrigel coated plates (Stem Cell Technologies) or FGF/KSR conditions on MEF substrate ...
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bioRxiv - Molecular Biology 2022Quote: ... were a gift from the Nik-Zainal lab (University of Cambridge, UK) and cultured on non-tissue culture treated plates (Stem Cell Technologies) pre-coated with 10 μg/mL Vitronectin XF (Stem Cell Technologies ...
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bioRxiv - Immunology 2022Quote: ... the media was aspirated from the transwell which was then transported to a new 24-well plate and 600 μL of PneumaCult™ALI Maintenance medium (STEMCELL Technologies) was added to the basolateral side ...
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bioRxiv - Immunology 2022Quote: ... The transwells were then moved to a new 24-well plate wherein each test well contained 600 μL of fresh PneumaCult™-ALI Complete Base Medium (STEMCELL Technologies) for the basolateral side ...
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bioRxiv - Molecular Biology 2021Quote: ... cell colonies were detached from the plate using 0.5 mM EDTA and placed in a low attachment 6-well plate (MERK) well in 1 mL of mTeSR1 medium (Stem cell technologies) containing 10 μM of Blebbistatin (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... to sort single cells into Matrigel-coated 96-well plates with each well containing 150 μl of mTeSR1 supplemented with CloneR (STEMCELL Technologies, #05888). Following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... hiPSCs were thawed into 6 well plates (TPP) that were pre-coated for 1h at RT with 10 μg/ml Vitronectin (Stem Cell Technologies) in CellAdhere dilution buffer (Stem Cell Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... the corrected and patient cell lines were maintained in plates coated with Geltrex (1:50 DMEM/F12) overnight using mTeSR (Stem Cell technologies). The media was refreshed daily for iPSCs ...
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bioRxiv - Cancer Biology 2020Quote: ... Appropriate numbers of total bone marrow cells were then exposed to various concentration of MMC and seeded into 6-well plates with MethoCult GF M3434 (Stem Cell Technologies) media following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... were cultured in clear 96-well Greiner COC plates which were plasma treated and coated with 0.1% gelatin (STEMCELL Technologies, Vancouver, Canada). iCell cardiomyocytes plating medium from the iCell Cardiomyocytes Media Kit was used to plate the cardiomyocytes at 60,000 cells/well and then exchanged with iCell Cardiomyocytes maintenance medium after 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: 2,500 cells were seeded into each well of an ultra-low attached 6-well plate in 2 mL complete MammoCultTM Medium (Stem Cell Technologies) based upon the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... 1 or 2 EBs were transferred to a well of a 24-well ultra-low attachment plate containing Induction Medium (Stem Cell Technologies). The EBs were kept in the induction Medium for 2 days and next they were transferred into Matrigel (Corning ...
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bioRxiv - Developmental Biology 2020Quote: ... Both hiPSCs and ESCs showed pluripotency markers and normal karyotype throughout the study (Figure S1A-B) and were grown on matrigel-coated plates in mTeSR medium (Stem Cell Technology) containing the Rock inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... 288 mio NPCs per axis were aggregated into 14400 organoids (20000 cells/per organoid) in Aggrewell800 6-well plates (Stem Cell Technologies). The day after ...
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bioRxiv - Genomics 2020Quote: ... a piece of frozen tumor tissue was placed into a well of a 6-well plate (Stem cell Technologies, catalog no. 38015) with 1 mL of either CST ...
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bioRxiv - Bioengineering 2021Quote: ... 1.5×10^6 cells were used for each targeting and were plated onto geltrex coated wells on 6-well plates in mTeSR with CloneR (Stemcell Technologies, 05889) following nucleofection ...
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bioRxiv - Genomics 2021Quote: ... The sorted cells were subsequently used as input for a second sort in which single cells were deposited into 384-well plate wells containing 20μl StemSpan™ SFEM II (Stem Cell Technologies) with 1X CD34+ Expansion Supplement (Stem Cell Technologies) ...
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bioRxiv - Genetics 2020Quote: ... emerging iPSC colonies that were large enough to isolate were manually picked using a sterile pulled glass pipette into VTN-coated 6-well tissue culture plates containing complete TeSR-E8 media (TeSR-E8 basal medium with 25X TeSR-E8 supplement) (Stem Cell Technologies) using a SMZ1000 Nikon dissecting microscope (Nikon ...
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bioRxiv - Developmental Biology 2022Quote: KOLF2 iPS cells were grown under feeder-free conditions on Matrigel-coated plates in TeSR™-E8™ (Stem Cell Technologies, #05990).
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bioRxiv - Synthetic Biology 2022Quote: Human iPSC line PGPC-17/11 (Personal Genome Project Canada) was cultured on Matrigel-coated plates in mTeSR Plus medium (STEMCELL Technologies 05825) and passaged with ReLeSR (STEMCELL Technologies 05873 ...
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bioRxiv - Neuroscience 2022Quote: ... the iPSCs were seeded in 6-well plates coated in phenol-red free Matrigel with hematopoietic differentiation media (StemCell Technologies, cat.no. 05310). The media was changed following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 15 min at 37°C and adherent aNSCs/NPCs were lifted from the plate using 1 mL of Accutase (STEMCELL Technologies, 07920) incubated for 5 min at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Definitive hindgut endoderm was generated as reported.8 Induced cells were harvested and seeded on AggreWellTM400 Microwell Plate (StemCell Technologies Cambridge, MA) at 3.6 million cells in each well for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... hESC colonies are passaged and small clusters are plated onto a 6 well plate at a density of ∼ 1-2 x 104 cells/well in TeSR-E8 media (StemCell Technologies, #5990). Following 24 hrs ...
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bioRxiv - Cell Biology 2024Quote: ... 200-500 µm EBs were picked under a dissecting microscope and transferred onto adherent tissue culture plates (2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL2 (Stem Cell Tech 05270), with 0.5 % Protein free hybridoma medium (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: MDA-MB-468 and mitomycin-C treated NIH-3T3 cells were plated at a ratio of 10:1 in AggreWellTM400 plates at approximately 50 cells/microwell according to the manufacturer’s instructions (#34415, Stemcell Technologies, Vancouver, BC) and grown for 5 days in tumor media containing 1μg/mL doxycycline ...
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bioRxiv - Cell Biology 2023Quote: ... hiPSCs were maintained on a Corning Matrigel (0.5 mg/ 6-well plate, dissolved in DMEM/F- 12 medium) cultured in mTeSR1 medium (StemCell Technologies, Vancouver, BC). Cells were passaged every 4 days using Versene solution (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... and 2.8×105 cells/cm2 were plated on Geltrex-coated plates in mTesR+ supplemented with ROCK inhibitor Y-27632 (10uM, StemCell Technologies, 72302). Neuronal induction was started 24h later (day 0 ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... after expansion the cells were prepared for embryoid body formation using the AggreWell™ EB 400 microwell plate following the manufacturers recommendations (StemCell Technologies). Cells were dissociated in Accutase (Innovative Cell Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... 5.8 × 105 cells in N2/B27 media and supplements were seeded into one well of a 6 well AggreWell™400 culture plate (STEMCELL Technologies) which was coated with 5% Pluronic acid F-127 (Sigma-Aldrich P2443 ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
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bioRxiv - Bioengineering 2023Quote: IMR90-4 iPSCs (WiCell, Madison, WI) were cultured on Matrigel-coated 6-well plates and supplemented daily with E8 medium (Stem Cell Technologies) as described (81) ...
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bioRxiv - Genomics 2023Quote: ... single hESC cells were plated at a density of approximately 5×105 per each well of a 6 well plate in the presence of ROCK inhibitor and exposed to commercially available mesoderm induction media (Stemcell Technologies 05221).
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bioRxiv - Molecular Biology 2023Quote: ... and plated (200,000 cells/well) on low attachment 12-well plates coated with poly-2-hydroxyethyl methacrylate in mTeSR1 Plus (STEMCELL Technologies, Vancouver, Canada) medium with 10 μM Y27632 (Selleckchem ...
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bioRxiv - Developmental Biology 2023Quote: PP cells were dissociated using TrypLE Express at 37°C for 2-3 mins and seeded in microwells of AggreWell 800 plates (STEMCELL Technologies, 34825), using the recommended procedure by the supplier ...
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bioRxiv - Neuroscience 2024Quote: ... the volume of cell suspension needed to create 500-cell spheroids was added to an AggreWell microwell plate (Stemcell Technologies Inc., Canada). The wells were then filled with NB to a volume of ∼2 mL ...
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bioRxiv - Neuroscience 2024Quote: ... gene targeted hPSCs were plated at a density of 250 cells per well of a 6-well plate in mTeSR1 medium supplemented with 1X CloneR (STEMCELL technologies, 05888) and incubated for 48 hours ...
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bioRxiv - Genomics 2024Quote: ... we seeded thawed iPSC at passage 12 (P12) or above in Matrigel-coated 6-well plates in mTESR1 media (STEMCELL Technologies (SCT), Vancouver ...
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bioRxiv - Bioengineering 2024Quote: hPSCs were maintained on Geltrex-coated plates in feeder-free mTeSR-1TM and mTeSR PlusTM media (Stemcell Technologies #85850 and #100-0276) using an established protocol (63) ...
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bioRxiv - Neuroscience 2024Quote: ... Positive cells were sorted into 96-well Matrigel-coated plate at 1 cell/well with 100 µl/well StemFlex and 1x CloneR2 (Stem Cell Technologies). 10-12 days later ...
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bioRxiv - Cancer Biology 2024Quote: Primary cells were seeded in 12-well plate at a density of 1×106 cells per well in 2 ml StemSpan™SFEM II media (STEMCELL Technologies) with growth factors cocktail (100 ng/ml SCF ...
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bioRxiv - Genomics 2024Quote: ... 4.5–9 x 104 cells were plated onto 12-well plates pre-coated with 80 µg/µl growth factor- reduced Matrigel in mTeSR1 (StemCell Technologies; 85850) and 10 µM Y-27632 ...
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bioRxiv - Bioengineering 2024Quote: ... was maintained in an iMatrix-511 silk (Elixirgen Scientific; NI511) coated 6-well plate using mTeSRTM Plus medium (Stemcell Technologies; 100-0276). The differentiation of iPSCs into EPCs was performed using a previously published protocol15 ...
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bioRxiv - Cell Biology 2024Quote: ... 500uL of the cell aggregate suspension were transferred to one well of a 6-well plate previously rinsed with Anti- Adherence Rinsing Solution (STEMCELL Technologies, 07010) containing 2.5mL of N2B27 media ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated at 8 × 104 cells per well on a Matrigel-coated 6-well plate in mTeSR1 supplemented 10 µM Y27632 (STEMCELL Technologies, 72307). The following day ...
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bioRxiv - Cell Biology 2024Quote: ... into the protein coated 96-well plates containing 100 µl intestinal organoid growth media supplemented with 10 µM Y-27 (Stem cell technologies). Growth of colonies were analysed using brightfield and fluorescent images with EVOS microscope ...
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bioRxiv - Biochemistry 2024Quote: ... according to the manufacturer’s instructions and seeded in 6-well cell culture plates (1.5 x 106 cells/well) in medium supplemented with 50 ng/mL M-CSF (#78057, STEMCELL Technologies, Cologne, Germany). Medium exchanges ...
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bioRxiv - Bioengineering 2024Quote: ... The brain organoid differentiation was modified from the cortical organoid protocol described in Trujillo et al6 and Fitzgerald et al.75 A 24-well AggreWell 800 plate (Stemcell Technologies, 34811) was prepared by adding Anti-Adherence Rinsing Solution (Stemcell Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... 96,000 human pluripotent stem cells were seeded on Geltrex (Thermo Fisher A14133-01)-coated plates in Stemflex with 10 µM ROCK inhibitor (Y27632) and after 24hours the medium was changed to TeSR-E6 (Stemcell Technologies, 05946) with 6μM CHIR99021 (Day0) ...