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Citations for Stemcell Technologies :
601 - 650 of 815 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissected prostate tissues were dissociated in DMEM containing 5% FBS and 1x collagenase/hyaluronidase (STEMCELL Technologies, Vancouver, BC, Canada), followed by trypsinization with 0.25% trypsin-EDTA (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... organoids were treated from days 3 - 5 with ROCKi-free neural differentiation medium supplemented with retinoic acid (RA) (Stemcell Technologies) at 0.25 μM and smoothened agonist (SAG ...
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bioRxiv - Cancer Biology 2020Quote: ... and the cells then dissociated in 2.5 mg/ml trypsin with 1 mM EDTA and 5 mg/ml dispase (STEMCELL Technologies) with 100 μg/ml DNaseI (Sigma ...
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bioRxiv - Neuroscience 2022Quote: hiPSCs were cultured at 37ºC and 5% CO2 in a humidified incubator (BINDER C150) in mTeSR1 medium (STEMCELL Technologies, #85850) on Matrigel (Corning ...
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bioRxiv - Genomics 2021Quote: ... The medium was changed every day and the cells were passed every 5-6 days using ReLeSR (Stem Cell technologies). 24h prior transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocyte specification was completed by 3 days of culture in RPMI B27-media with 5 μM IWP-4 (STEMCELL Technologies), followed by another 7 days of culture with RPMI B27+ (RPMI 1640 GlutaMAX + 2% B27 supplement with insulin (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... The cells were incubated at 37°C and 5% v/v CO2 until confluency in PneumaCultTM-ExPlus media (STEMCELL Technologies) for 4-7 days before being switched to ALI culture conditions by removing the apical media and feeding the basal side with PneumaCultTM ALI medium (STEMCELL Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was replaced on d1 with RPMI/B27™ minus insulin supplemented with 5 µM IWP-4 (STEMCELL Technologies). On d3 medium change was performed using RPMI/B27™ minus insulin without any supplementation ...
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bioRxiv - Neuroscience 2022Quote: ... NPCs were plated at 1.25×5 cells/cm2 on Matrigel hESC-qualified Matrix-coated wells and maintained in Neural Progenitor medium (StemCell Technologies) with daily complete media change ...
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bioRxiv - Physiology 2022Quote: ... Cells were cultured in mTESR1 media at 37°C at 5% CO2 and passaged using accutase (Stemcell Technologies, Cat. # 07920) and media supplemented with Y-27632 2HCl (ROCK ...
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bioRxiv - Immunology 2024Quote: ... mice and bone marrow progenitors were plated on non- tissue treated dishes and differentiated into BMDM during a 6-day culture in complete Dulbecco’s modified Eagle’s medium (DMEM) containing 5% FBS and supplemented with 60 ng/ml recombinant murine M-CSF (Stemcell Technologies) in a water saturated atmosphere at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5% O2 for 14 d before scoring colonies using the human mPB program on a STEMvision imager (StemCell Technologies).
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bioRxiv - Bioengineering 2023Quote: High cell density (4×107 cells/mL) hPSC strips containing 1.5mg/mL collagen with 5% Geltrex were collected in an Anti-Adherence Rinsing Solution (STEMCELL Technologies) treated 60mm Petri dish ...
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bioRxiv - Developmental Biology 2022Quote: ... IPSC colonies were picked and passaged every 5 to 7 days after transfer to the mTeSR culture system (Stemcell Technologies).
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bioRxiv - Cancer Biology 2022Quote: ... containing 2% FBS and the tissue was further dissociated by treatment with dispase (5 mg/mL)/DNaseI (0.1 mg/mL) solution (STEMCELL Technologies). The cell suspension was filtered using a 40 µm cell strainer (Falcon ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Genetics 2022Quote: ... HSPCs were plated at a concentration of 5×102 cells/mL in a methylcellulose-containing medium (GFH4435, Stem Cell Technologies) under conditions supporting erythroid and granulo-monocytic differentiation ...
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bioRxiv - Cell Biology 2024Quote: ... the monolayers were washed twice with PBS then incubated in dispase solution (5 units/mL in HBSS, Stemcell Technologies #07913) for 80 minutes at 37 °C to detach the monolayers from the plate ...
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bioRxiv - Neuroscience 2024Quote: ... hiNS were supplied with a continuous perfusion of carbogen (95% O2/ 5% CO2) infused BrainPhys Imaging Optimized media (STEMCELL Technologies) and the following intracellular solution was used for microglial patch-clamp recordings ...
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bioRxiv - Neuroscience 2024Quote: ... Neural rosettes were isolated after 5 days in culture by selection using STEMdiff Neural Rosette Selection Reagent (STEMCell Technologies, 05832). The resulting NPCs were cultured on PLO and laminin-coated plates ...
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bioRxiv - Bioengineering 2024Quote: ... Fresh gonadal and perirenal adipose tissues were minced with scissors and incubated in collagenase type II (2 mg/mL; StemCell Technologies, CA) and DNase I (20 µg/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... were directly sorted into a 96-well tissue culture plate containing 100 μL of differentiation media: StemSpan (Catalog #09650, StemCell Technologies), 1% Penicillin+Streptomycin ...
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bioRxiv - Immunology 2022Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Harvested spheroids then were transferred into 10-cm ultra-low adhesion plates in Essential 6 medium containing dual SMAD pathway inhibitors [100 nM LDN193189 (STEMCELL Technologies) and 10 μM SB431542] and 2.5 μM XAV939 (Cayman) ...
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bioRxiv - Neuroscience 2021Quote: ... Day 8 differentiated Ngn2 neurons were re-seeded at a density of 1×105 cells per 100 µL droplet in CM2 media consisting of BrainPhys (STEMCELL Technologies), 1x penicillin/streptomycin ...
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bioRxiv - Pathology 2021Quote: ... washed 5x with water and prefilled with 100 uL per well of Neural Coating Medium (NCM) comprising of Brainphys medium (STEMCELL Technologies) with 1X B27+Vit.A ...
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bioRxiv - Immunology 2021Quote: ... before centrifugation at 300 xg for 10 minutes followed by resuspension in 100 μl of CryoStor-CS10 freezing media (StemCell Technologies). The UVC preparations for use in non-human primate studies were analyzed for endotoxin levels (Wickham Laboratories Ltd ...
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bioRxiv - Immunology 2021Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... the mice were irradiated with a dose of 100 cGy per mouse and injected intravenously with 0.2 million of HSCs (purchased from Stemcell Technologies Inc.). After 8–10 weeks ...
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bioRxiv - Cancer Biology 2023Quote: ... Further T cells isolation from human PBMCs was done with EasySep™ Human T Cell Isolation Kit (STEMCELL Technologies, 100-0695) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: ... For immunolabelling embryos were washed 4 times with PTwH (PBS/0.2% Triton-X-100/10 µg/mL heparin (Stemcell Technologies, #07980)) for 1 h each at 37°C and incubated with primary antibody (PE-labelled rabbit anti-CD31/PCAM1 (1:100 ...
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bioRxiv - Developmental Biology 2023Quote: ... 70 µl of media were removed from each well and replaced by 100 µl embryoid body (EB) formation medium (Cerebral organoid kit, cat. n. 08570, StemCell Technologies). On day 2 ...
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bioRxiv - Immunology 2024Quote: ... were differentiated into TH2 T cell subtype by incubation with specific supplement for TH2 differentiation (mouse IL-2, mouse IL-4 and rat anti mouse IFNγ with 1:100 dilution (STEMCELL Technologies)) and incubated for 3 days on plates coated with aCD3 and aCD28 antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Developmental Biology 2024Quote: ... for 7 mins at 37 °C and 12,000 cells in 100 µl STEMdiff kit EB formation media (Stem Cell Technologies, 08570) supplemented with 50 µM Rock inhibitor Y-27632 (Stem Cell Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Minced tissues were transferred with help of a sterile wide bore 200 µl pipette tip into a 1.8 ml cryo vial containing 1.2 ml A-mTE-D1 (300 IU ml-1 collagenase IV mixed with 100 IU ml-1 hyaluronidase; Stem Cell Technologies, 07912 ...
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bioRxiv - Genetics 2023Quote: ... washed five times with water and prefilled with 100 µl per well of neural coating medium comprising Brainphys medium (STEMCELL Technologies) with 1× B27 plus vitamin A ...
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bioRxiv - Cancer Biology 2022Quote: CD34-positive and Lin-negative cells from PBMC were single-cell sorted into 96-well plates containing 100 μL of free medium consisting of StemSpam SFEMII (Stemcell Technologies) with human cytokines SCF ...
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bioRxiv - Cancer Biology 2022Quote: ... non-TC-treated 96-well U-bottom plates were treated with 100 μL of anti-adherence rinsing solution (StemCell Technologies 07010) for 1 h ...
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bioRxiv - Immunology 2022Quote: HLA-A2+ monocytes were differentiated into moDCs for 7 days with GM-CSF (50 ng/ml) and IL-4 (100 ng/ml; both STEMCELL Technologies); media and cytokines were refreshed every 2–3 days ...
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bioRxiv - Immunology 2023Quote: ... Remaining whole blood was diluted 1:1 with RPMI + 1% FBS + 2mM L-Glutamine + 100 U Penicillin/Streptomycin (R1) and layered onto SEPMATE tubes (STEMCELL Technologies) containing lymphoprep gradient (STEMCELL Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1.5×105 day 6 BMDM cells were mixed and seeded together with 300 dissociated GC organoids in 100 µl RGF BME domes in 24-well plate wells and incubated for 72 h in 100 µl IntestiCultTM organoid growth medium (STEMCELL Technologies) containing 20% L929 conditioned medium and 20 ng/ml IL4 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then dissociated and resuspended in cloning media consisting of mTeSR-Plus supplemented with 1X CloneR2 (StemCell Technologies; 100-0691) and mixed gently with a P1000 pipette followed by passing through a 40 μm cell strainer in order to generate a single-cell suspension ...
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bioRxiv - Cell Biology 2024Quote: ... In some experiments we also used an additional commercially available ENR medium (here denoted cENR; IntestiCult Human organoid differentiation medium; #100-0214, STEMCELL Technologies) with 100U/ml penicillin-streptomycin ...
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bioRxiv - Immunology 2024Quote: ... CD14-enriched cells were resuspended in ImmunoCult™ XF supplemented with 100 U/mL penicillin-streptomycin and cultured in the presence of 50 ng/mL GM-CSF and 100 ng/mL IL-4 (both from STEMCELL Technologies) for 7-days ...
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bioRxiv - Neuroscience 2024Quote: ... 100 mm petri dishes using complete mTeSR Plus medium (mTeSR Plus Basal medium and mTeSR Plus 5X supplement; 100-0276, StemCell Technologies). The medium was exchanged daily until the hiPSCs reached approximately 80% confluence ...
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bioRxiv - Cell Biology 2024Quote: ... the media was changed to remove the ROCK inhibitor and was maintained for 6 hours in in mTeSR plus (Cat# 100-0276, StemCell Technologies). The media was then replaced with DMEM/F12 (Cat# 11320033 ...
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bioRxiv - Neuroscience 2024Quote: Undifferentiated iPSCs and iE cells were incubated at 37°C in Gentle Cell Dissociation Reagent (Stem Cell Technologies CAT#100-0485) for 5 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... The plasmids were cotransfected into iPSC cells with Lipofectamine Stem Reagent (ref. STEM00015, Thermo Fishser Scientific) in mTeSR media (ref. 100-0276, Stemcell Technologies) supplemented with 10µM Y-27632 for 24 hs ...
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bioRxiv - Cancer Biology 2024Quote: Control and ammonia-treated hepatospheres were dissociated into single cells using an enzyme-free dissociation reagent (StemCell Technologies, Cat. 100-0485), counted ...