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Citations for Stemcell Technologies :
551 - 600 of 815 citations for Locostatin CAS 90719 30 5 100% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The medium was changed every day and cells were passaged when 70% confluent using ReleSR (STEMCELL Technologies, #100-0484) at a 1:20 ratio ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD11B-FITC (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Immunology 2023Quote: ... and residual natural killer cells were depleted using 100 μL/mL of the CD56 Positive Selection Cocktail (STEMCELL Technologies). Monocytes (5ξ106/vial ...
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bioRxiv - Developmental Biology 2024Quote: ... resuspended in 100 μL phosphate buffered saline (PBS) without Ca2+ and Mg2+ (D-PBS; Stem Cell Technologies, cat.# 37350) and 20 μL aliquots were prepared and stored at -80°C.
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bioRxiv - Genetics 2023Quote: ... and fed every other day with stem cell media: mTeSR Plus with supplement (STEMCELL Technologies; cat. no. 100-0276) and 0.5% penicillin-streptomycin (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... H1 human embryonic stem cells (WiCell, WA01) were routinely cultured as colonies in mTeSR Plus (STEMCELL Technologies, 100-0276) with 0.2% penicillin/streptomycin (BioConcept ...
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bioRxiv - Bioengineering 2024Quote: ... HPCs were diluted to 50,000 cells per well in 100 µL of Media B (STEMdiff Hematopoietic Kit, STEMCELL Technologies) in a 96-well plate ...
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bioRxiv - Neuroscience 2020Quote: ... Primary mouse microglia and M-MØP cells were exposed at set time points to anti-FcγRII/III (2.4G2) antibody at 5 µg/ml (Stemcell technologies), LPS (50 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... mESCs were cultured in feeder free conditions with 5% CO2 at 37°C on 0.1% gelatin (StemCell Technologies, 7903) coated plates in 2i medium supplemented with 2% mES certified FBS (Gibco 16141061) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were seeded at 5 × 105 cells/ml and grown in Neurocult mouse neurobasal medium (Stem Cell Technologies, 5700) supplemented with 10 ng/ml hEGF (Lonza ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 naïve CD4+ T cells from HIV− or HIV+ donors were isolated by magnetic negative selection (StemCell Technologies), then cultured at 106 cells/mL in 96-well plates using R-10 media (RPMI-1640 media supplemented with 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using ReLeSR (Stemcell Technologies, 05872).
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bioRxiv - Molecular Biology 2020Quote: ... CD34+ HSPCs were cultured at 2.5×105–5×105 cells/mL in StemSpan SFEM II (STEMCELL Technologies, Vancouver, Canada) base medium supplemented with stem cell factor (SCF)(100ng/mL) ...
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bioRxiv - Neuroscience 2023Quote: ... the hiNPCs were singularized with Accutase for 10 min at 37 °C and 5 % CO2 (#07920, Stemcell Technologies, Canada) and centrifuged (300 g ...
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bioRxiv - Cell Biology 2023Quote: ... 1% (v/v) B27™ minus insulin) supplemented with 5 µM CHIR99021 and 33.3 ng Activin A (STEMCELL Technologies). Medium was exchanged daily without CHIR99021 supplementation ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x 105 CD3+ T cells were plated in 0.5 ml T cell expansion medium (Stemcell Technologies, Cologne, Germany) with 50 IE/ml IL-2 ...
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bioRxiv - Cancer Biology 2024Quote: ... mTeSR was changed every other day and cells were passaged every 3–5 days using ReLeaSR (StemCell Technologies #05872). All cell cultures were maintained in a humidified incubator with 5% CO2 at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... mesoderm was carried out for 5 days and ectoderm for 7 days using STEMdiff Trilineage Differentiation Kit (STEMCELL Technologies). Alternatively ...
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bioRxiv - Cell Biology 2023Quote: ... After 5 weeks the H5100 medium was replaced with Methocult H4434 Classic methylcellulose medium (100µl/ well, STEM CELL technologies). Wells that contained at least one colony after 10-14 days were considered positive.
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bioRxiv - Cancer Biology 2022Quote: ... We grew cells in a 5% CO2 incubator in a serum-free NeuroCult NS-A basal medium (STEMCELL Technologies) supplemented with NeuroCult™ Proliferation Supplement (STEMCELL Technologies) ...
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bioRxiv - Bioengineering 2022Quote: iPSC lines were maintained and expanded at 37°C in 5% CO2 in mTeSR™Plus medium (Stemcell Technologies) on 1:100 diluted Geltrex (Thermo Fisher)-coated plates with daily medium changes and passaging every 3-4 days with releasing agent ReLeSR (Stemcell Technologies).
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bioRxiv - Cell Biology 2022Quote: ... Red blood cells were removed using the ammonium chloride solution: 5 ml of 0.8% Ammonium Chloride (StemCell Tech. 07800) was added to the suspension and incubated for 10 min with intermittent mixing ...
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bioRxiv - Developmental Biology 2022Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemicals were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 300g for 5 mins and RBC lysed using Ammonium Chloride Lysis Buffer (Stem Cell Technologies) as per manufacturer protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Dispase II at 5 U/ml stock solution in Hanks’ balanced saline was from Stemcell Technologies (cat. no. 07913). The following chemical compounds were prepared and used according to the manufacturers’ guidelines ...
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bioRxiv - Cancer Biology 2024Quote: ... Two million human tumor cells were cultured under mammosphere conditions using 5 mL of complete Mammocult medium (StemCell Technologies) in ultra-low attachment plates (Corning ...
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bioRxiv - Synthetic Biology 2024Quote: ... coated tissue culture plates and passaged regularly as cell aggregates every 4-5 days using RelesR (STEMCELL Technologies, 05872). Identity of H1 cells was authenticated by the supplier ...
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bioRxiv - Genomics 2024Quote: ... Organoids followed by incubation for 5 minutes at 37°C with resuspension with Accutase (Stemcell Technology, Cat No.07922) were dissociated into 10-15 cell clusters by pipetting 10-20 times ...
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bioRxiv - Developmental Biology 2024Quote: RUES2 hESCs were cultured in a humidified incubator at 37°C and 5% CO2 in mTeSR1 (StemCell Technologies, 85850) on growth factor-reduced Matrigel-coated (Corning ...
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bioRxiv - Molecular Biology 2020Quote: ... frozen cord blood and Plerixafor- and/or G-CSF-mobilized peripheral blood (AllCells, Alameda, CA, USA and STEMCELL Technologies, Vancouver, Canada), frozen Plerixafor- and/or G-CSF-mobilized peripheral blood of patients with SCD ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured in StemMacs iPS-Brew media (Miltenyi Biotech, Auburn, CA) and routinely passaged utilizing Gentle Cell Dissociation Reagent (STEMCELL Technologies) onto Matrigel-coated (1 mg ml-1 ...
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bioRxiv - Neuroscience 2024Quote: Large pieces of similar size (diameter 200 µm) were dissected manually from iPSC colonies growing in mTeSR Medium (Stemcell Technologies, CA). The pieces were let form embryoid bodies over 5 days ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in 100-200 μl of Iscove’s Modified Eagle Medium (IMDM) supplemented with 20% serum substitute (BIT 9500; Stemcell Technologies), 1 μg/ml low-density lipoprotein (LDL ...
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bioRxiv - Neuroscience 2023Quote: ... Stem cell culture was done using a commercially available media kit (mTeSR Plus; Cat No. 100-0276, STEM CELL Technologies) following the manufacturer’s published protocol [32] ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and blocked with CD32 antibody (2 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60012) for 15 minutes at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were harvested and incubated with CD45-APC (1 µl per 50,000 cells in 100 µL, STEMCELL Technologies Catalog #60018AZ) and CD34-PE (1 µl per 50,000 cells in 100 µL ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cells were routinely cultured on matrigel in mTeSR1 or mTeSR Plus (Stem Cell Technologies cat #85850 or cat #100-0276). Culture and in vitro differentiation of iPS cells into six cell types (motor neurons (MN) ...
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bioRxiv - Cell Biology 2023Quote: ... Twenty-four hours later 10/50/500 non irradiated CD34+ cells or 1,500/7,500/15,000 irradiated (3Gy) CD34+ cells per well were plated in 100 μl MyeloCult H5100 medium (STEM CELL technologies). Once a week half medium replacement was done ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... iPSCs were plated on Matrigel coated plates at ∼400k cells/cm2 in mTeSR plus medium (STEMCELL Technologies, Cat#100-0276). From day 0 to day 3 ...
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bioRxiv - Neuroscience 2023Quote: ... Day 8 Ngn2 neurons were then seeded at a density of 100,000 cells per well in 100 µl droplets of CM2 Brainphys media [Brainphys (STEMCELL technologies), 1x Glutamax ...
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bioRxiv - Bioengineering 2022Quote: ... incubated at 1:100 in DMEM for 1 h on TCPS plates) and passaged in clumps using ReLeSR (STEMCELL Technologies), all according to manufacturer guidelines ...
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bioRxiv - Neuroscience 2024Quote: ... The KnockOut medium was supplemented with a two-inhibitor cocktail (2i) containing 100 nM LDN-193189 (Stemcell Technologies, Vancouver, Canada) and 10 μM SB-431542 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2024Quote: ALI cultures on the apical chamber of transwell inserts were infected with SARS-CoV-2 viral inoculum (MOI of 0.1; 100 µl/well) prepared in PneumaCult ALI (STEMCELL technologies) media ...
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bioRxiv - Cell Biology 2024Quote: ... washed twice with ice-cold PBS and resuspended in IntestiCult™ Organoid Differentiation Medium (Human) (ODM, STEMCELL Technologies, 100-0214) supplemented with or without 10 µM IWP-2 (STEMCELL Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were suspended in Hanks Balanced Salt Solution (HBSS) supplemented with 5% human serum and 1.5 µg/mL anti-human CD32 antibody (Clone IV.3; STEMCELL Technologies) and then stained with designated antibodies for 1-2 hours on ice prior to sorting on a Becton Dickinson FACSAria™ Fusion or FACSAria™ III sorter ...
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Pluripotent stem cell SOX9 and INS reporters facilitate differentiation into insulin-producing cellsbioRxiv - Developmental Biology 2021Quote: ... centrifuged at 50 x g for 5 min and subsequently dissociated by incubation with gentle cell dissociation solution (StemCell Technologies) for 15 min and additional T/E for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... The microcapsules carrying HUES-8 cells were collected into 15 mL tube filled with 5 mL E8 medium (STEMCELL Technologies), then distributed into a 6 well-plate at density of 2000 capsules/well and incubated at 37 □ with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... Rescue of epithelial integrity was induced by threating hiPSCs for 1 day with LPA (StemCell Technologies, ref. 72694, 5 µM). Chemical disruption of epithelial integrity was induced by threating hiPSCs for 1 day with Blebbistatin (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Cell Biology 2022Quote: ... They were patterned into definitive endoderm using the STEMdiff Definitive Endoderm Kit per manufacturer’s instructions over 5 days (StemCell Technologies). Cells were passaged on day 5 of differentiation using GCDR ...