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Citations for Stemcell Technologies :
6151 - 6200 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... PBMCs were obtained by leukapheresis and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the instructions of the manufacturer (StemCell Technologies, Vancouver, BC, Canada) and were activated with phytohemagglutinin-L (10 μg/ml ...
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bioRxiv - Immunology 2021Quote: Naïve IgM+IgD+B cells were purified by negative selection using the EasySepTM human naive B cell enrichment kit (19254; StemCell Technologies) from healthy subject PBMCs ...
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bioRxiv - Immunology 2021Quote: ... density gradient centrifugation with SepMate tubes (Stem Cell Technologies) as per manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... samples were enriched for live cells using a Dead Cell Removal (Annexin V) Kit (Stem Cell Technologies), then labeled with unique anti-human TotalSeq-B hashing antibodies (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... Both cell types were passaged every 7 days or as they reached 70-80% confluency using ReLeSR (STEMCELL Technologies). All iPSC and ESC lines used for these studies are listed in Table 1 along with the method of reprogramming and institution they were reprogrammed at ...
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bioRxiv - Immunology 2021Quote: ... iPSC were dissociated to single cells using ACCUTASE (StemCell Technologies) and pelleted at 300xg for 5 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... coated 6-well plates in mTeSR Plus (STEMCELL Technologies, Vancouver, BC, Canada) with medium changes every other day ...
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bioRxiv - Immunology 2021Quote: ... ESC were maintained on Matrigel coated plates in mTeSR1 (STEMCELL Technologies) with daily medium changes ...
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bioRxiv - Immunology 2021Quote: ... The CD34+ cells were cryopreserved at 10e6 cells/mL in Cryostor CS10 (STEMCELL Technologies) according to the manufacturer’s protocol and the remaining hemogenic endothelium was cultured in 2mL of monocyte factory medium ...
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bioRxiv - Immunology 2021Quote: ... ESC or iPSC were grown to ~75% confluency then dissociated into aggregates using ReLeSR (STEMCELL Technologies) and suspended in mTeSR Plus ...
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bioRxiv - Immunology 2021Quote: ESC or iPSC were differentiated to hematopoietic progenitors following the manufacturer’s instructions for the STEMdiff Hematopoietic Kit (STEMCELL Technologies). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: Cells were pre-treated with 10 µM Y-27632 (STEMCELL technologies) for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... coated plates in complete mTeSR1 medium (StemCell Technologies). Cultures were monitored and areas of spontaneous differentiation were removed ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were passaged when they reached ∼80-90% confluence using ReLeSR (StemCell Technologies). Induction of pluripotency was validated by the expression of OCT4 and NANOG via immunofluorescence (data not shown) ...
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bioRxiv - Neuroscience 2021Quote: ... regularly passaged as aggregates using ReLeSR (StemCell Technologies), and cultured on Geltrex-coated plates (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2021Quote: ... 20 ng/mL FGF2 and 20 ng/mL EGF) supplemented with ROCK inhibitor (Y-27632, Stemcell Technologies) at each single-cell passage.
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bioRxiv - Neuroscience 2021Quote: ... The isogenic and non-isogenic iPSCs were cultured in mTesR1 or mTesR Plus medium (StemCell Technologies), regularly passaged as aggregates using ReLeSR (StemCell Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... The culture medium was then replaced with mTesR1 or mTesR Plus medium (StemCell Technologies, Vancouver, Canada) and MEFs were replaced by a Geltrex basement membrane matrix (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Dispase (5 U/mL) (STEMCELL Technologies, Cat. 07913)
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bioRxiv - Synthetic Biology 2021Quote: ... the cerebral and ocular organoids were cultured in mTeSR1 medium (Stem Cell Technologies, cat. no. 85850). Human ES cell (H1 WA01 and H9 WA09 ...
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bioRxiv - Microbiology 2021Quote: ... buffy coats (from anonymous healthy blood donors from Mississippi Blood Center) were separated by Ficoll and CD4+ T-cells purified using RosetteSep Human CD4+ T-cell enrichment kit (STEMCELL Technologies). CD4+ T-cells cells were maintained in Roswell Park Memorial Institute 1640 medium (RPMI ...
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bioRxiv - Synthetic Biology 2021Quote: ... 20 mM cyclic Pifithrin-Alpha (Stem Cell Technologies cat# 72062) and 20 ng/ml human recombinant bFGF (Stem Cell Technologies cat# 78003) ...
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bioRxiv - Synthetic Biology 2021Quote: ... And then single cell clones were first detached using 20 μl Accutase (STEMCELL Technologies cat# 07920) and neutralized with 20 μl growth media ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 20 ng/ml human recombinant bFGF (Stem Cell Technologies cat# 78003). For 10 gBlocks pool ...
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bioRxiv - Microbiology 2021Quote: ... after Lymphoprep density gradient (Stemcell Technologies) separation of PBMCs ...
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bioRxiv - Microbiology 2021Quote: ... were generated by positive selection of CD14+ cells from PBMCs using the EasySep Human CD14 Positive Selection Kit II (STEMCELL Technologies, Cat: 17858) and differentiating cells for at least 7 days in RPMI supplemented with 10% Human AB Serum (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... Negative selection was performed using EasySep Human CD4+ T Cell Enrichment Kit (Stemcell Technologies) after Lymphoprep density gradient (Stemcell Technologies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and Aldefluor (Stem cell Technologies). For compensation and gating purposes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Pooled breast epithelial single cells from 15 patients were cultured in MammoCult media (Stem Cell Technologies, Vancouver, Canada) and treated with curcumin ...
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bioRxiv - Microbiology 2021Quote: ... we first isolated CD4+ T cells from 10M PBMC by negative selection using the EasySep Human CD4+ T cell Enrichment Kit (STEMCELL Technologies, Cat #: 19052). This yielded a mean of 1.6 (range 1.3-2.0 ...
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bioRxiv - Microbiology 2021Quote: ... in mTeSR Plus medium (STEMCELL Technologies, Vancouver, Canada) at 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... the matrigel was dissolved and the extracted organoids were dissolved in Accutase (Stemcell technologies). The cells were fixed with PBS for 10 min at 4°C and washed with cold PBS containing 2% FBS ...
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bioRxiv - Microbiology 2021Quote: ... in mTeSR Plus with 10 mM Y-27632 (STEMCELL Technologies). The next day ...
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bioRxiv - Systems Biology 2021Quote: ... and 2ug/mL heparin) (STEMCELL Technologies #05751). We incubated the plate of cells for 36 hours prior to the start of the experiment at 37°C and 5% CO2 in a tissue culture incubator ...
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bioRxiv - Systems Biology 2021Quote: ... The iPSCs were maintained in mTeSR1 medium (Stemcell Technologies) and routinely passaged every 4~6 days with Versene (EDTA ...
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bioRxiv - Microbiology 2021Quote: ... Embryoid bodies were then embedded in 15 ul of Matrigel (Stem Cell Technologies, Cat. #354277) and media changed to 1:1 mixture of Neurobasal medium (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... in mTeSR medium (Stem Cell Technologies, Cat. #85851). Cortical organoids were generated by an optimized protocol57 where patterned embryoid bodies were expanded for four days in N2 medium ...
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bioRxiv - Microbiology 2021Quote: ... Cells were maintained in NeuroCult™ NS-A Differentiation Medium Human (STEMCELL Technologies) for up to 14 days.
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bioRxiv - Microbiology 2021Quote: ... Cells were grown in suspension as spheres in flask in NeuroCult-XF Proliferation Medium Human (STEMCELL Technologies) with EGF and bFGF (10 ng/ml each ...
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bioRxiv - Molecular Biology 2021Quote: ... Total CD4+ T cells were separated by negative selection using RosetteSep™ Human CD4+ T Cell Enrichment Cocktail (StemCell Technologies). Primary CD4+ T cells were plated at a concentration of 1,5×10 6 cells/mL left overnight for recovery and infected ...
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bioRxiv - Molecular Biology 2021Quote: ... purified by density gradient centrifugation using SepMate™ tubes and Lymphoprep™ media (Stem Cell Technologies, Vancouver, BC), were resuspended in sterile phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC). For intracellular staining ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were resuspended in PBS+2% FBS and then processed for FACS staining or frozen in 10% Cryostor (Stem Cell Technologies, Vancouver, BC).
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bioRxiv - Immunology 2021Quote: ... Neutrophils were isolated from the remaining blood pellet via negative selection using the EasySep Direct Neutrophil Isolation Kit (STEMCELL Technologies, Cat# 19666) following manufacturer’s directions ...
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bioRxiv - Neuroscience 2021Quote: ... 1% (v/v) N2 Supplement-A (StemCell Technologies), 20 ng/ml Recombinant Human Brain-Derived Neurotrophic Factor (StemCell Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ng/ml Recombinant Human Glial-Derived Neurotrophic Factor (StemCell Technologies), 1 mM Dibutyryl-cAMP (PeproTech) ...
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bioRxiv - Neuroscience 2021Quote: ... with the addition of the following supplements 2% (v/v) NeuroCultTM SM1 Neuronal Supplement (StemCell Technologies), 1% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... filled with cutting solution before transferring to individual wells in a 12 well plate filled with BrainPhys neuronal medium (StemCell Technologies) with the addition of the following supplements 2% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... 20 ng/ml Recombinant Human Brain-Derived Neurotrophic Factor (StemCell Technologies), 20 ng/ml Recombinant Human Glial-Derived Neurotrophic Factor (StemCell Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nM ascorbic acid (StemCell Technologies), 1x penicillin streptomycin (ThermoFisher ...