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Citations for Stemcell Technologies :
6101 - 6150 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: ... and IWP2 (2μM, Stem cell technology). ALP activity in treated conditions is expressed in terms of fold change with respect to the control medium with appropriate amount of DMSO ...
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bioRxiv - Neuroscience 2021Quote: ... in i3Neuron Culture Media: BrainPhys media (STEMCELL Technologies, Cat. No. 05790) supplemented with 1x B27 Plus Supplement (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... iPSC colonies were treated with Gentle Dissociation Reagent (GDR, StemCell technologies, 07174) for 7 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... and differentiated in Pneumacult-ALI medium (#05001; StemCell Technologies) or custom ALI media (Spirovation ...
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bioRxiv - Microbiology 2021Quote: ... pieces of intestinal resections were washed thoroughly in ice-cold PBS and epithelial crypts were subsequently dissociated using Gentle Cell Dissociation Reagent (STEMCELL Technologies, Vancouver, BC, Canada) by nutating at 4°C for 30 minutes followed by trituration ...
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bioRxiv - Microbiology 2021Quote: ... the hiPSCs were allowed to self-aggregate in 96-well plates for five days using Neural induction medium (Stem cell technologies) as described earlier (Gabriel et al. ...
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bioRxiv - Microbiology 2021Quote: ... and stem cells were passaged using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech#05872). Cultures were maintained in an undifferentiated state ...
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bioRxiv - Microbiology 2021Quote: ... or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were negatively isolated using the RosetteSep™Human CD4+ T Cell Enrichment Cocktail (Stem Cell Technologies) or the EasySep™ Human Naïve CD4+ T Cell Isolation Kit (Stem Cell Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Neuronal differentiation was performed using the protocol as described [59] with a modification that the cells were frozen at the neural progenitor stage at day 8 in CryoStor CS10 (Stem Cell Technologies #07931). Cells were thawed into LIA medium (ADF supplemented with 2mM Glutamax ...
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bioRxiv - Microbiology 2021Quote: ... with daily feeding of mTeSR1™ (Stem Cell Technologies cat#85850) [59] ...
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bioRxiv - Microbiology 2021Quote: ... 5% CO2 for 15-20 minutes to allow the Matrigel to polymerize and then 550 μl of pre-warmed complete IntestiCult Growth Medium (mouse) (6005; STEMCELL Technologies) containing 500 nM Y-27632 (10005583 ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was removed and organoid pellets were re-suspended in Cryostor CS10 cryopreservation medium (STEMCELL Technologies) at approximately 500-1000 organoids/ml before being transferred to a cryovial ...
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bioRxiv - Genomics 2021Quote: ... diluted in CellAdhere Buffer (Stem Cell Technologies, Catalog Number ...
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bioRxiv - Genomics 2021Quote: ... Cells were subcultured once a week with ReLeSR for two weeks (Stem Cell Technologies; Catalog Number: 05872) before equal numbers of the hiPSC lines were combined and plated together ...
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bioRxiv - Genomics 2021Quote: ... with Rock inhibitor Y-27632 (10µM final concentration; Stem Cell Technologies; Catalog Number: 72304) on Costar non-treated 6 well polystyrene plates (Catalog Number ...
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bioRxiv - Genomics 2021Quote: ... on Costar non-treated 6 well polystyrene plates (Catalog Number: 3736; Lot Number: 30417038) coated with Vitronectin XF (Stem Cell Technologies; Catalog Number ...
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bioRxiv - Genomics 2021Quote: ... 35 nM UM171 ((STEMCELL technologies, Vancouver, Canada), 100 ng/ml each of hFLT-3L ligand ...
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bioRxiv - Genomics 2021Quote: ... and 4) one cord blood (CB001) collected with informed consent by StemCell Technologies (catalog #70007) (Table S1) ...
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bioRxiv - Genomics 2021Quote: ... Cord blood MNCs (4) were received frozen and then CD34+ selected using the EasySep human whole blood CD34 positive selection kit (Stemcell Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cells were centrifuged (350 x g for 5 minutes) and resuspended in 1 mL of prewarmed dispase (#07913, Stemcell Technologies) and 100 μL of DNase I (#07900 ...
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bioRxiv - Genomics 2021Quote: ... filtered through a 40 μm cell strainer (#27305, Stemcell Technologies), centrifuged (350 x g for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... and 100 μL of DNase I (#07900, Stemcell Technologies) and passed 5 times through a 27G syringe needle ...
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bioRxiv - Genomics 2021Quote: ... cells were centrifuged (350 x g for 5 minutes) and resuspended in 5 mL of prewarmed 0.25% trypsin/EDTA (#07901, Stemcell Technologies) at 37°C for 5 minutes ...
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bioRxiv - Genomics 2021Quote: ... Prostate lobes were minced with a sterile razor blade and resuspended in collagenase/hyaluronidase (#07912, Stemcell Technologies) diluted in DMEM/F-12 (#36254 ...
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bioRxiv - Genomics 2021Quote: ... diluted in DMEM/F-12 (#36254, Stemcell Technologies) at 37°C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... neural tissues were embedded in Matrigel droplets and moved to ultralow attachment 6-well plates and fed expansion media (Stemcell Technologies). Day 10 organoids were transferred to a rocker to elicit the circulation of nutrients and prevent organoids from sticking to the dish ...
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bioRxiv - Microbiology 2021Quote: ... iPSCs were plated at 9,000 cells per well onto 96-well ultralow attachment plates for embryoid body (EB) formation and grown in EB formation media (StemCell Technologies). At day 5 ...
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bioRxiv - Microbiology 2021Quote: Cortical organoid cultures were differentiated from the healthy iPSC cell line according to the specification of the cerebral organoid kit from StemCell Technologies (#08570) that relies on an established protocol (54) ...
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bioRxiv - Microbiology 2021Quote: ... the induction of neural epithelium was initiated by moving the EBs into an ultralow attachment 24-well plate and feeding with induction media (StemCell Technologies). On day 7 ...
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bioRxiv - Microbiology 2021Quote: ... 100 ng/ml fibroblast growth factor (FGF; Stem Cell Technologies), and 5 μg/ml heparin (Millipore Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... and CD4+ T lymphocytes were purified from resting PBMCs by negative selection using immunomagnetic beads per the manufacturer’s instructions (StemCell Technologies, Vancouver, BC) and were activated with phytohemagglutinin-L (10 μg/mL ...
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bioRxiv - Genetics 2021Quote: ... 100ng/ml human interleukin 6 (hIL-6) (all obtained from EuroBiosciences) and 35nM UM171 molecule (Stem Cell Technologies). Cells were cultured under normoxic conditions ...
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bioRxiv - Genomics 2021Quote: ... were sorted using a BD FACSAria™ Fusion Cell Sorter into ice-cold Serum-Free Expansion Medium (SFEM) supplemented with 100 ng/ml SCF and Flt3 (Stem Cell Technologies), 20 ng/ml IL-3 ...
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bioRxiv - Genomics 2021Quote: ... G-CSF and TPO (Stem Cell Technologies). Cells were plated in Corning Costar Ultra-Low Attachment 96-well flat-bottom plates (Sigma ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... The CD4+ T cells were purified from 50 × 106 PBMCs using EasySep™Human CD4+ T cell isolation kit (STEMCELL Technologies). Primary CD4+ T cells were cultured at 37°C in a 5 % (v/v ...
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bioRxiv - Microbiology 2021Quote: ... The cells were cultured and differentiated using Complete PneumaCult™-Ex plus medium and PneumaCult™-ALI-S Maintenance medium (Stemcell Technologies, Vancouver, Canada), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... Negative selection using the RosetteSep Human T cell Enrichment kit (Stem Cell Technologies) and SepMate-50 tubes was performed to purify primary human T cells ...
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bioRxiv - Cancer Biology 2021Quote: ... Israel directly after surgery and were transported to our laboratory on ice in sterile tubes containing HypoThermosol® FRS medium (StemCell Technologies). Tissue was processed within 72 hours of surgery ...
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coat (Research Blood Components) using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... coated plates using mTSER media (StemCell Technologies, Vancouver, Canada, #05850), which was replaced every day ...
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bioRxiv - Bioengineering 2021Quote: ... and plated on Matrigel-coated plates at ±8.75 × 104 cells/cm2 in mTeSR medium (Stem Cell Technologies) supplemented with RevitaCell (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were isolated using SepMate PBMC Isolation Tubes (Stemcell Technologies, Cat no. 86450) according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... The negative fraction of the above selections was divided into two aliquots that were subjected to negative immunomagnetic isolation of either B cells (EasySep Human Pan-B cell Enrichment Kit, Stemcell Technologies, #19554) or T cells (EasySep Human T cell Isolation Kit ...
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bioRxiv - Immunology 2021Quote: ... or T cells (EasySep Human T cell Isolation Kit, Stemcell Technologies, #17951). After isolation ...
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bioRxiv - Immunology 2021Quote: ... counted and treated with 10 U ml−1 DNAse I (Stemcell Technologies, #) for 15 min at RT in order to prevent cell clumping ...
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bioRxiv - Immunology 2021Quote: ... plasma cells were isolated using the EasySep Human CD138 Positive Selection Kit II (Stemcell Technologies, #17877) for analysis in a companion study (manuscript in preparation) ...
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bioRxiv - Immunology 2021Quote: ... CD4 cells were isolated using the Easy-Sep CD4+ T cell isolation kit (Stemcell Technologies), plated on a tissue culture-treated plate pre-coated with neutravidin (Thermo) ...
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bioRxiv - Immunology 2021Quote: ... Using EasySep mouse B cell enrichment kit and manufacturer’s protocol (StemCell Technologies Inc., Tukwila, WA, USA), B cells were isolated by negative selection and resuspended in FACS buffer (PBS with 2% Fetal calf serum ...