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Citations for Stemcell Technologies :
5751 - 5800 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... commercially available cryopreserved PBMCs (Stemcell Technologies, Vancouver, Canada) and patient 7316-3778 ...
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bioRxiv - Cancer Biology 2021Quote: ... PBMCs were purified on day 0 by Ficoll density gradient centrifugation (SepMate, Stemcell Technologies, Vancouver, Canada) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Aldehyde dehydrogenase activity was measured first by trypsinizing melanocytes as above and then using the ALDEFLUOR kit (Stemcell Technologies, 01700) as described in the manufacture’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Human primary total T cells (CD4 and CD8) were isolated from normal healthy donors following leukapheresis by negative selection using RosetteSep kits (Stemcell Technologies, Vancouver, CA). All specimens were collected with protocol approved by University Review Board and written informed consent was obtained from each donor ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 ng/ml H-PDGF-BB (Shenandoah #100-16) and 2 μg/ml heparin (Stem Cell Technologies #07980). All cells were grown in a standard humidified cell culture incubator at 37 °C and 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: Peripheral blood mononuclear cells (PBMC) were isolated from fresh human buffy coats using immunomagnetic negative selection (Stem Cell Technologies) and cultured in RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... a second bead-based purification step was included according to the manufacturer’s instructions (EasySepTM Human Isolation Kit from STEMCell Technologies, Cologne, Germany). While density centrifugation led to 90% pure PMN ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged using Gentle Cell Dissociation Reagent (Stem Cell Technologies, #7174). iPSCs were confirmed to be karyotype normal and maintained below passage 10 and all the cell lines were routinely checked and were negative for mycoplasma.
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bioRxiv - Cell Biology 2021Quote: ... Y-27632 (10 µM, Stem cell Technologies, #72304). After 24h cells were treated with a 1:1 mixture of DMEM/F12 + GlutaMAX (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... the medium was replaced with STEMdiff APEL2 medium (Stem Cell Technologies, #05270) supplemented with FGF9 (200 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies, #3450). Pellets were incubated with CHIR99021 (5 µM ...
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bioRxiv - Cell Biology 2021Quote: ... was maintained in mTeSR1 medium (Stem Cell Technologies, #85870) in T25 flasks pre-coated with Matrigel (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... in the STEMdiff APEL2 medium (Stem Cell Technologies, #05270) for four days ...
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bioRxiv - Cell Biology 2021Quote: ... cells were dissociated with Accutase (Stem Cell Technologies, #07920) and 2 × 105 cells were seeded on Type I Collagen-coated 6-well dishes and cultured until day fourteen in DMEM/F12+GlutaMAX medium supplemented with 10% FBS (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF iPSCs were plated in a T25 flask in the mTeSR1 medium with ROCK Inhibitor Y-27632 (10 µM, Stem cell Technologies, #72304). After 24 hours ...
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bioRxiv - Cell Biology 2021Quote: ... in T25 flasks pre-coated with Matrigel (Stem Cell Technologies, #354277). Cells were passaged using Gentle Cell Dissociation Reagent (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... A total number of 3.75 × 105 ThF human iPSCs were seeded in a Matrigel-coated T25 flask in mTeSR1 medium (Stem Cell Technologies, #85870) with ROCK inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... cells were dissociated into single cells using AccutaseTM (Stem Cell Technologies, #07920). 5 × 105 cells were pelleted at 350x g for 2 min and transferred onto a 6-well transwell membrane (Stem Cell Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... bovine serum albumin (BSA; 1 %; Stem Cell Technologies), mSCF (10 ng/mL) ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated from whole blood using ficoll density gradient centrifugation in a SepMate tube (Stem Cell Technologies) following 1:2 dilution with RPMI ...
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bioRxiv - Immunology 2021Quote: ... (2) ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 1X Penicillin Streptomycin solution (ICM) ...
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bioRxiv - Genomics 2021Quote: ... tissue was viably frozen by placing 4 biopsies in 500 ml of CryoStor CS10 freezing media (STEMCELL Technologies). Tubes were inverted and then placed on ice for up to 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... Sorted HSPCs from donor AX001 were plated onto CFC media MethoCult H4435 (STEMCELL Technologies) and colonies were picked following 24 days in culture.
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bioRxiv - Genomics 2021Quote: ... Uniform and reproducible EBs were achieved using AggreWell 400 plates (STEMCELL Technologies, cat. no. 34415) containing 1200 microwells ...
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bioRxiv - Genomics 2021Quote: ... containing 100uL of supplemented StemPro media (Stem Cell Technologies) (MEM media) ...
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bioRxiv - Genomics 2021Quote: ... MEM media contained StemPro Nutrients (0.035%) (Stem Cell Technologies), L-Glutamine (1% ...
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bioRxiv - Immunology 2021Quote: ... pre-loaded with Lymphoprep™ (07801, Stemcell Technologies) for PBMC isolated by density gradient centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Diluted blood was transported in 50 ml SepMate™ tubes (86450, Stemcell Technologies) pre-loaded with Lymphoprep™ (07801 ...
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bioRxiv - Immunology 2021Quote: ... 250 nM LDN (StemCell Technologies), 30 nM PMA (Sigma Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... and plated on Matrigel-coated plates at a density of 5 x 105 cells per cm2 in mTESR1 medium (StemCell Technologies) supplemented with 10 ng/mL of the RHO/ROCK pathway inhibitor ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were isolated from peripheral blood of donor 2395 via negative selection using the RosetteSep Human CD8+ T Cell Enrichment Cocktail (StemCell Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... iPSC colonies were manually isolated then expanded on Matrigel-coated dishes in mTeSR1 medium (StemCell Technologies, Vancouver, Canada).
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bioRxiv - Immunology 2021Quote: ... the medium was changed to STEMdiff Mesoderm Induction medium (StemCell Technologies) and the medium was changed daily ...
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bioRxiv - Immunology 2021Quote: ... donor 2395 iPSC colonies were dissociated to single cells with Accutase (StemCell Technologies) and plated on Matrigel-coated plates at a density of 5 x 105 cells per cm2 in mTESR1 medium (StemCell Technologies ...
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bioRxiv - Immunology 2021Quote: ... Donor 2395 iPSC were maintained on Matrigel-coated 6 well plates in mTeSR+ medium (StemCell Technologies) and differentiated to sBC as previously described with some modifications (29) ...
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bioRxiv - Immunology 2021Quote: ... EasySep Buffer (Cat. No. 19762, 19666, and 20144 from STEMCELL Technologies), and Calcein AM (Cat no ...
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bioRxiv - Immunology 2021Quote: ... S3d) for the studies involving glucose exposure using EasySep™ Mouse Neutrophil Enrichment Kit (STEMCELL Technologies), as per manufacture’s protocol and as described previously (25 ...
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bioRxiv - Immunology 2021Quote: ... human neutrophils were purified from blood using the EasySep™ Human Neutrophil Enrichment Kit (STEMCELL Technologies), according to manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... Diluted and filtered blood was then carefully layered on top of Lymphoprep (STEMCELL Technologies, #07851). The gradient was centrifuged at 800 g for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... T-cells were isolated with an EasySep CD3+ enrichment kit from StemCell Technologies (Vancouver, BC Canada), per the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: 0.25-0.5 million non-parenchymal cells were used with the EasySepTM Mouse T cell Isolation Kit (Stemcell Technologies, Cat.# 19851) for negative selection of T cells ...
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bioRxiv - Immunology 2021Quote: Human NK were isolated from discarded Leukopaks from healthy volunteer blood donors using the RosetteSep™ human NK enrichment protocol (StemCell Technologies), followed by Ficoll (GE Healthcare ...
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bioRxiv - Immunology 2021Quote: ... or EasySep™ Release Mouse PE Positive Selection Kit (Stem Cell Technologies, Cat no. 17656) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were pre-enriched from LeukoPaks by negative selection (RosetteSep (StemCell Technologies)) and then stained with CD8-APC/Cy7 (Clone SK1 ...
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bioRxiv - Immunology 2021Quote: ... with 0.25% collagenase I (Stem Cell Technologies), then again using enzyme-free cell dissociation buffer (Gibco) ...
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bioRxiv - Immunology 2021Quote: ... Huh7 and HepG2 and autologous CD8+ T cells (pre-activated with ImmunoCult™ Human CD3/CD28 T Cell Activator, Stemcell Technologies, Canada according to the manufacturer’s instruction) ...
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bioRxiv - Immunology 2021Quote: ... ICM T cells were stimulated with ImmunoCult™ Human CD3/CD28 T Cell Activators (25 μl/ml, STEMCELL Technologies). TAC T cells were stimulated with T Cell CD3/CD28 TransAct™ ...
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bioRxiv - Immunology 2021Quote: ... Negative selection of NK was performed on EasySep™ Human NK Cell Enrichment Kit system (StemCell Technologies). 2.106 sorted NK cells were cultured with 100 Gy-irradiated EBV-LCL cells in a 1:20 ratio ...
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bioRxiv - Immunology 2021Quote: PBMCs were isolated from six human peripheral blood leukapheresis packs (STEMCELL Technologies) using Ficoll gradient density centrifugation ...
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bioRxiv - Immunology 2021Quote: ... Glucose-free ImmunoCult™-XF T Cell Expansion Medium (STEMCELL Technologies) was supplemented with 24 mM [U-13C]glucose ...