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Citations for Stemcell Technologies :
5651 - 5700 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... lineage negative cells were enriched from total bone marrow isolated from femurs and tibias of donor mice (6-12 weeks of age) using the EasySep Mouse Hematopoietic Progenitor Cell Isolation Kit (STEMCELL Technologies) in conjunction with the RoboSep-S (STEMCELL Technologies ...
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Genetically engineered microglia-like cells have therapeutic potential for neurodegenerative diseasebioRxiv - Neuroscience 2021Quote: ... in conjunction with the RoboSep-S (STEMCELL Technologies) automated cell isolation machine and confirmed by flow analysis ...
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bioRxiv - Neuroscience 2021Quote: ... and subsequent passaging continued to use ReLeSR (STEMCELL Technologies). iPSC lines without karyotypic abnormalities were used and this was verified by G-band karyotyping performed by the Centre for Applied Genomics (Hospital for Sick Children) ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs (Wicell UCSD021i-3-9) were differentiated into Hematopoietic progenitor cells (HPCs) using the STEMdiff™ Hematopoietic Kit (Stem Cell Technologies # 05310) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... On day -1 cells were plated into mTeSR1 medium with 0.5 μM Thiazovivin (STEMCELL technologies # 72252) onto hESC-matrigel coated (Corning # 354277 ...
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bioRxiv - Neuroscience 2021Quote: ... IPSCs cells were grown in mTeSR1 (STEMCELL technologies Catalog # 85850) and passaged with 0.5 mM EDTA in PBS (-Ca+2/Mg+2) ...
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bioRxiv - Neuroscience 2021Quote: ... The differentiation of HPCs into IMGLs was performed using the STEMdiff™ Microglia Differentiation Kit and the STEMdiff™ Microglia Maturation Kit (STEMCELL technologies # 100-0019 and #100-0020) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2021Quote: ... ascorbic acid (200 μM, #72132; StemCell Technologies), GDNF (20 ng/ml ...
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bioRxiv - Neuroscience 2021Quote: ... at 37 °C for 1 min and seeded on AggreWell 800 (#34815; StemCell Technologies) in Essential 8 medium with ROCK inhibitor Y-27632 (10 μM ...
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bioRxiv - Neuroscience 2021Quote: ... brain-derived neurotrophic factor (BDNF, #78133; StemCell Technologies), ascorbic acid (200 μM ...
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bioRxiv - Neuroscience 2021Quote: ... Dissociated single cells were seeded on AggreWell 800 (#34815, StemCell Technologies). 1000 single cells were added per well in Essential 8 medium supplemented with 10μM ROCK inhibitor Y-27632 ...
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bioRxiv - Neuroscience 2021Quote: ... in Essential 8 medium with ROCK inhibitor Y-27632 (10 μM, #72308, STEMCELL Technologies). After 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... GDNF (20 ng/ml, #78139.1, StemCell Technologies) and cAMP (1 μM ...
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bioRxiv - Neuroscience 2021Quote: ... and supplemented with 20 ng/ml BDNF and 20 ng/ml Neurotrophin-3 (NT3, #78074; STEMCELL Technologies) until day 43 ...
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bioRxiv - Neuroscience 2021Quote: ... 10 µM SB431542 (StemCell Technologies, 72234) and 2 µM XAV939 (StemCell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Spinal cord isolation was performed using contents of ‘NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (StemCell Technologies)’ ...
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bioRxiv - Neuroscience 2021Quote: ... 75% of the medium was replaced by BrainPhys medium (Stemcell technologies) supplemented with B-27 (Gibco) ...
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bioRxiv - Neuroscience 2021Quote: ... in mTeSR™1 medium (StemCell Technologies, 85850) and passaged in the presence of ROCK Inhibitor InSolution™ Y-27632 (Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... on matrigel coated plates and split using ReleSR at room temperature (Stem cell technologies). Stem cells were differentiated to retinal organoids using an optimized protocol as previously described see also supplementary methods (17).
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bioRxiv - Neuroscience 2021Quote: The mCar-GFP and Crx-mCherry iPSC lines (kind gift from Olivier Goureau – see (23)) were maintained in mTeSR1 (Stem cell technologies) on matrigel coated plates and split using ReleSR at room temperature (Stem cell technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... integrated and inducible pure human cortical-like glutamatergic neurons (i3Ns) was completed in two weeks in BrainPhys media (STEMCELL Technologies, 05790) (Figure ...
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bioRxiv - Neuroscience 2021Quote: ... The iPSCs were expanded and maintained in mTeSR1 medium from Stem Cell Technologies (see iPSC Media in Supplementary Table 1).
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bioRxiv - Neuroscience 2021Quote: ... maintained in Phenol Red-free BrainPhys media (STEMCELL Technologies, 05791), imaged on a Cytation 5 cell-imaging multi-mode reader (BioTek ...
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bioRxiv - Neuroscience 2021Quote: ... and then transferred to an Aggrewell 800 plate (StemCell Technologies cat. no. 34815) pre-coated with Anti-adherence rinsing solution (StemCell Technologies cat ...
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bioRxiv - Molecular Biology 2021Quote: ... and activated with Immunocult™ human CD3/CD28 T cell activator (Stemcell Technologies, Vancouver, Canada), in the RPMI-1640 media supplemented with 2mM L-Glutamine ...
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bioRxiv - Molecular Biology 2021Quote: ... and human recombinant IL-2 (Stemcell Technologies, Vancouver, Canada). Activated CD4+ T cells were transfected with pVax-CasRx and gRNA plasmids by nucleofection using Lonza 4d nucleofector ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were differentiated into hematopoietic progenitor cells (HPCs) using the STEMdiff Hematopoietic Kit (StemCell Technologies). Floating HPCs were then collected and plated at 50,000 cells/mL in STEMdiff Microglia Differentiation media (StemCell Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... (2017)57 or the newly available differentiation kit by STEMCELL Technologies based on the McQuade et al ...
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bioRxiv - Molecular Biology 2021Quote: PBMC were purified from blood samples from GD patients using Lymphoprep™ reagent and SepMate™ tubes (Stemcell Technologies, Vancouver, Canada). Lymphoprep™ was added to the lower compartment of the SepMate tube ...
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bioRxiv - Molecular Biology 2021Quote: IPSCs were dissociated with Gentle Cell Dissociation Reagent (Stem Cell Technologies) while Accutase® Cell Dissociation Reagent (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: The iPSCs were seeded on Matrigel-coated dishes and expanded in mTESR1 (Stemcell Technologies) or Essential 8 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... or transparent centres were manually removed from the dish prior to dissociation with Gentle Cell Dissociation media (Stemcell Technologies). The IPSCs were then seeded and differentiated into cortical or dopaminergic neuronal progenitors or neurons.
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bioRxiv - Molecular Biology 2021Quote: ... the pooled samples were diluted 1:1 with PBS+2% FBS and loaded into SepMate-15™ tubes with 4.5 mL of Lymphoprep™ with a density of 1.077g/mL (StemCell Technologies). Cells were centrifuged at 1200x g for 20min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 μM ROCK inhibitor (STEMCELL Technologies) in order to form EBs ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 ng/ml of stem cell factor (Stemcell Tech), 30 ng/ ml of Flt3-L (PeptroTech) ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... The resulting CD34+ HSPCs were cultured in HSPC media: StemSpan SFEM II (STEMCELL Technologies Inc.) supplemented with 1% penicillin/streptomycin/amphotericin B and SFT cytokines ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: Frozen human peripheral blood CD19+ B cells were purchased from StemCell Technologies (Vancouver, Canada). Cells were thawed per manufacturer’s instructions and cultured as previously described.60 Briefly ...
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Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... and magnetic bead selection using an EasySep™ Human CD34 Positive Selection Kit (STEMCELL Technologies Inc.). The resulting CD34+ HSPCs were cultured in HSPC media ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10uM ROCK inhibitor (STEMCELL Technologies, Y-27632). Cells were cultured in E14 standard medium for additional 7 days and triplicated for genomic DNA extraction ...
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bioRxiv - Molecular Biology 2021Quote: ... and 10uM ROCK inhibitor (STEMCELL Technologies, Y-27632). Cells were then cultured in standard E14 medium with 1ug/mL of puromycin ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 days post-transfection in 1 ml STEMdiff Neural Progenitor Medium (Stemcell Technologies) cells were washed by DPBS and 2 wells of the plate were put together and collected by centrifugation at 10.000 rpm for 2 min generating 3 biological replicates ...
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bioRxiv - Molecular Biology 2021Quote: ... Growth media was NeuroCult NSC Basal Medium (Mouse) containing NeuroCult Proliferation Supplement (Mouse) (StemCell Technologies), 0.2% heparin solution (Stem-Cell Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... recombinant human basic FGF (20ng/mL, all from Stem Cell Technologies) and 10 ng/ml laminin (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... were suspended in Intesticult (INT) human organoid growth medium (Stem Cell Technologies) proliferation medium ...
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bioRxiv - Neuroscience 2021Quote: ... iPSCs were maintained in mTeSR1 medium (Stemcell Technologies) on Matrigel-coated plates (Fisher Scientific CB-40230) ...
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bioRxiv - Neuroscience 2021Quote: ... the differentiating iNeurons were dissociated using Accutase (STEMCELL Technologies) and resuspended in a culture medium consisting of Neurobasal media (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... inguinal lymph nodes from immunized or naïve mice or live LN sections were incubated in 1 mL of digestion solution comprised of RPMI1640 medium containing 0.8 U/mL Dispase (07913, Stemcell Technologies), 0.1 mg/mL Collagenase D (11088858001 ...
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bioRxiv - Immunology 2021Quote: ... CD34+ precursors cells were isolated from PBMCs (EasySep™ Human CD34 Positive Selection Kit, STEMCELL Technologies). CD34+ cells were grown under serum-free conditions using StemSpan™ medium (STEMCELL Technologies ...
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bioRxiv - Immunology 2021Quote: ... CD34+ cells were grown under serum-free conditions using StemSpan™ medium (STEMCELL Technologies) supplemented with recombinant human IL-6 (50 ng mL-1 ...
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bioRxiv - Immunology 2021Quote: ... Isolation of T and B cells were performed according to the manufacturer’s protocols (Stemcell Technologies).