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Citations for Stemcell Technologies :
501 - 550 of 4196 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... CD3+ T cells were activated for 72 hours with ImmunoCult T cell activator (STEMCELL Technologies) following manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: Human induced pluripotent stem cells (iPSCs) were maintained in mTeSR+ medium (Stem Cell Technologies #05825) on tissue culture-treated plates coated with Matrigel (Corning #354277) ...
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bioRxiv - Cancer Biology 2023Quote: Primary cells are resuspended in a 3:4 solution of Mammocult medium (Stem Cell Technology) and Matrigel (Corning)17,26,29 ...
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bioRxiv - Immunology 2023Quote: ... CD14+ Mo were FACS sorted from frozen human bone marrow mononuclear cells (Stem Cell Technologies) on a BD FACS Melody Cell Sorted (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: T cells were enriched using the Easy Sep Human T cell Isolation Kit (Stemcell Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies, cat #07930) at −80 °C until use (50,000 cells per tube) ...
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Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... Stimulation was done using ImmunoCult human CD3/CD28/CD2 T Cell Activator (Stem Cell Technologies) in non-polarizing conditions using anti-IL-4 (5 μg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... NHBE cells were maintained in filter-sterilized PneumaCult-Ex-PLUS Medium (Stem Cell Technologies #05040) supplemented with 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2023Quote: ... iPSCs were dissociated into single cells using Gentle Cell Dissociation Reagent (STEMCELL Technologies, cat # 07174) for 8 minutes at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... these cells were used in 3 mL of methylcellulose medium (H4435; Stem Cell Technology, Canada) plated in a 35-mm culture dish (Grainger) ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were harvested and dissociated into single cells by incubation with Accutase (STEMCELL Technologies) for 10 min at 37 °C ...
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bioRxiv - Genomics 2023Quote: ... during their expansion phase the cells were cultured in StemSpan H3000 medium (Stem Cell Technologies) supplemented with 100 ng/mL stem- cell factor (SCF ...
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bioRxiv - Developmental Biology 2023Quote: Expanding PP cells were routinely frozen at later passages using mFreSR (Stem Cell technologies, 05854), supplemented with 20 μM ROCKi ...
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bioRxiv - Cell Biology 2023Quote: Thawed CD34+ cells were cultured in StemSpan SFEM II serum-free medium (Stem cell technologies) supplemented with recombinant cytokines (Peprotech Asia) ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were cultured at 37°C with 5% CO2 in StemSpan (Stem Cell Technology #09650) supplemented with TPO (0,125ng/mL) ...
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bioRxiv - Immunology 2024Quote: ... NK cells were isolated by EasySep™ Human NK Cell Isolation Kits (STEMCELL Technologies, 17955) and activated by 100 Gy irradiated K562 Clone 9 feeder cells ...
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bioRxiv - Cell Biology 2023Quote: ... 2.25·106 cells/well were seeded onto AggreWell TM800 24-well plates (Stem Cell Technologies) in 2mL SCDM (described above) ...
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bioRxiv - Immunology 2024Quote: ... T cells were isolated by EasySep™ Human T Cell Isolation Kits (STEMCELL Technologies, 17951). Naïve T cells were isolated by EasySep™ Human Naïve Pan T Cell Isolation Kits (STEMCELL Technologies ...
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bioRxiv - Immunology 2020Quote: ... 1 x 105 T cells purified from spleen and lymph nodes with the EasySep Mouse T cell Isolation Kit (Stem Cell Technologies; Vancouver, BC, Canada) were cultured with 4 x 105 T-depleted splenocytes in 96-well round bottom plates in R10 media with 1 μg/mL αCD3 ...
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bioRxiv - Neuroscience 2019Quote: ... splenocytes were further separated by negative selection using the EasySep T cell isolation kit designed for CD3+ T cells (Stem Cell Technologies, Vancouver, BC, Canada) following the manufacturer’s guidelines for manual separation ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells at 106 cells/mL were stimulated with 25 μL/mL ImmunoCult™ Human CD3/CD28 T Cell Activator (STEMCELL Technologies, Vancouver, Canada, catalogue #10991). At day 3 post-stimulation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were dissociated with Gentle Cell Dissociation Reagent (STEMCELL Technologies 07174 and passaged 1:4 (C1) or 1:6 (C2 ...
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bioRxiv - Developmental Biology 2021Quote: ... and purified for live cells using the EasySep Live/Dead magnetic selection kit (Stem Cell Technologies). Suspensions were passed through a 40-μm mesh filter to obtain single cells.
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bioRxiv - Genomics 2020Quote: ... Cells were first differentiated into definitive endoderm using the STEMdiff Definitive Endoderm Kit (Stem Cell Technologies) for 72 hours and subsequently dissociated with GCDR and passaged as small clumps into growth factor reduced Matrigel-coated (Corning ...
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bioRxiv - Immunology 2021Quote: T cells were isolated from naïve spleens with the EasySep T cell isolation kit (STEMCELL Technologies). For each mouse 4×106 naïve T cells were injected intravenously via the tail ...
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bioRxiv - Immunology 2019Quote: ... Pure cell populations were isolated using EasySep Human negative selection kits (STEM CELL technologies, >95% purity). In addition ...
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bioRxiv - Cell Biology 2019Quote: ... Hematopoietic stem cells were enriched using the EasySep Mouse Hematopoietic Progenitor Cell Enrichment kit (Stemcell Technologies) and 1*106 HSCs were administered via tail vein injection to recipient mice ...
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bioRxiv - Cancer Biology 2019Quote: Cells in semi-solid media were prepared using the Neurocult NCFC Assay Kit (Stem Cell Technologies) per manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... The electroporated cells were resuspended with Stemspan II media with CC100 cytokine cocktail (Stem Cell Technologies). Two guides targeting GFI1B coding regions (sgRNA1 ...
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bioRxiv - Cancer Biology 2020Quote: CD4+ T cells were isolated using the EasySep Mouse CD4+ T-cell isolation kit (Stemcell Technologies). The CD4+ T cells were then added in a 1:1 ratio to cultured CMT167 cells ...
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bioRxiv - Genomics 2019Quote: Mouse bone marrow cells (2.5×104) suspended in methylcellulose semisolid medium (Methocult M3231) (Stem Cell Technologies) were plated in 35-mm culture dishes in the presence of 0.5 % FBS ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Bioengineering 2020Quote: ... Human peripheral blood mononuclear cells were isolated from fresh human blood using Ficoll (Stem cell technologies) density centrifugation ...
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bioRxiv - Synthetic Biology 2021Quote: ... The cell pellet was resuspended at 10,000 cells/µl in BrainPhys (Stemcell Technologies Australia, Melbourne, Australia) neural maintenance media with Rho Kinase Inhibitor IV (Stemcell Technologies Australia ...
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bioRxiv - Biophysics 2020Quote: ... cell monolayers were maintained for 20-30 days in STEMDiff cardiomyocte maintenance media (STEM CELL Technologies), on EStim classic 48 well MEA plates (Axion Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were cultured in Mammocult media supplemented with 4 μg/ml Heparin (Stem Cell Technology), 0.5 μg/ml hydrocortisone and 10% Mammocult Proliferation Supplement (Stem Cell Technology) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were adjusted to 1×105 cells/ml final concentration in H4230 methylcellulose medium (STEMCELL Technologies) supplemented with 10% 5637-CM as a stimulant and then plated in 35-mm petri dishes in duplicate and allowed to grow for 7 days in a humidified CO2 incubator (5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: Cells were dissociated from the well using Gentle Cell Dissociation reagent (StemCell Technologies, Cat#: 100-0485). Total proteins were extracted by adding 1ml of cold 10% TCA to the cell pellets ...
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bioRxiv - Cell Biology 2021Quote: ... Cell colonies were separately dissociated and transferred into 6-well plates using ReLeSR (Stem Cell Technologies). Differentiated cells were removed by using a pipette tip to minimize spontaneous differentiation ...
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bioRxiv - Microbiology 2020Quote: ... CD45− cells were magnetically sorted using Ter119/CD45-depletion kit (#19771; Stem Cell Technologies, Vancouver, BC). Next ...
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bioRxiv - Microbiology 2021Quote: ... and stem cells were passaged using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech#05872). Cultures were maintained in an undifferentiated state ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were cultured in ImmunoCult cell culture media (Stemcell Technologies, Cat no. 10981) supplemented with 35 ng/ml human IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... B cells were purified using negative magnetic isolation kit (Miltenyi Biotec Inc. or Stem Cell technologies) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... bulk CD4+ T cells were isolated using the EasySep CD4+ T cell Enrichment Kit (StemCell Technologies). Isolated CD4+ T cells were maintained in RPMI medium supplemented with 10% fetal bovine serum and 1% penicillin and streptomycin (R10 medium) ...
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bioRxiv - Cancer Biology 2020Quote: ... Then cells were re-suspended in 500μl and counted in 500μl ammonium chloride (Stem Cell Technologies) using the Vicell XR (Beckman Coulter) ...
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bioRxiv - Developmental Biology 2022Quote: ... at which point cells were split into new wells using ReLeSR passaging reagent (Stem Cell Technologies) with 2 μM ROCK inhibitor (Thiazovivin/TZV ...
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bioRxiv - Cancer Biology 2022Quote: Bone marrow cells were subjected to RBC lysis using Ammonium Chloride lysis buffer (Stem Cell Technologies), then incubated with anti-mouse direct lineage depletion cocktail (Miltenyi ...
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bioRxiv - Immunology 2022Quote: NK cells from spleen were enriched by the EasySep Mouse NK cell isolation kit (STEMCELL Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Sorted cells were plated in triplicate in semi-solid methylcellulose medium (Methocult M3434; Stem Cell Technologies) at 1000 cells/mL ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was changed every day and cells were passaged using gentle cell dissociation reagent (Stemcell Technologies) or singularised using accutase (Stem Cell Technologies) ...