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Citations for Stemcell Technologies :
751 - 800 of 4196 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... CD4+ T cells were isolated using the EasySep Human CD4+ T cell Isolation Kit (Stemcell Technologies, 17952) and subsequently activated using the T Cell Activation/Expansion Kit (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and stem cells were passaged weekly using enzyme free dissociation reagent ReLeSR™ (Stem Cell Tech #05872). Cultures were maintained in an undifferentiated state ...
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bioRxiv - Neuroscience 2022Quote: ... Cell plating: Cell plating medium consisted of 95 mL of BrainPhys Neuronal Medium (STEMCELL Technologies, Vancouver, Canada), 2 mL of iCell Neuronal Supplement B (FCDI) ...
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bioRxiv - Cancer Biology 2023Quote: ... The corpus was cut into small pieces and incubated with Gentle Cell Dissociation Reagent (STEM Cell Technology) for 10 min ...
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bioRxiv - Immunology 2023Quote: ... B cells were isolated using an EasySep Human Pan-B Cell Enrichment kit (Stemcell Technologies, Vancouver, Canada).
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bioRxiv - Bioengineering 2023Quote: ... They were expanded in bronchial epithelial cell growth media (BEGM) [35] or Ex Plus (Stem Cell Technologies) medium for 1 week before being cryopreserved ...
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bioRxiv - Developmental Biology 2023Quote: ... at which time cells were washed with PBS and incubated in ReleSR (Cat. # 05872, Stem Cell Technologies) for 1 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were generated by seeding single-cell suspension of iPSC in AggreWell-800 plates (Stem Cell Technologies) in STEMdiff Neural Induction Medium (NIM ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were resuspended with 0.5 mL of Complete MesenCultTM-ACF Chondrogenic Differentiation Medium (Stem Cell Technology) supplemented with antibiotics and centrifuged at 300 × g for 10 min to obtain a cell pellet ...
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bioRxiv - Cell Biology 2023Quote: ... Sorted cells were plated in Methocult optimum media (2-3 X 103 cells/well; Stemcell Technologies, H4034) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Cells were enriched for CD8+ population using the EasySep Human CD8+ T Cell Isolation Kit (Stemcell Technologies) and CD8+ cells were used for analysis.
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bioRxiv - Neuroscience 2023Quote: ... iPSC-derived hematopoietic progenitor cell differentiations were completed with STEMdiff Hematopoietic Kit (Stem Cell Technologies, cat#5310) where cells are fed with Media A (day 0-2 ...
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bioRxiv - Immunology 2023Quote: ... and T cells were purified using the EasySep™ Human T Cell Isolation Kit (StemCell Technologies, 17951). Once purified ...
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bioRxiv - Immunology 2024Quote: ... and differentiated into monocyte-derived dendritic cells (moDCs) using the ImmunoCult Dendritic Cell Culture Kit (STEMCELL Technologies), as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were frozen at 2.5e6 or 5E6 cells/mL in CryoStor CS10 (STEMCELL Technologies, Cambridge, MA).
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bioRxiv - Cancer Biology 2024Quote: ... at a density of 4-5×105 cells per well in Myelocult medium (H5100, Stem Cell Technology). On the morning ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells were isolated using EasySep Human Naïve CD4+ T Cell Isolation Kit (Stemcell technologies), according to manufacturer’s indications.
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bioRxiv - Bioengineering 2024Quote: ... T cells were isolated using the EasySepTM Human T cell isolation kit (StemCell Tech., Saint-Egrève, France) and cultured in complete X-Vivo15 medium ...
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bioRxiv - Cancer Biology 2022Quote: ... all the unwanted cells were labelled with magnet conjugated antibody cocktail which is separated using their EasySep magnetic stand leaving CD4 or CD8 cell in suspension using the vendor supplied EasySep Human CD4/CD8 T Cell Iso Kit (Stem Cell Technologies, 17952 for CD4 and 17953 for CD8). This negative selection approach results in isolation of untouched CD8 or CD4 T cells and stored frozen with 10% DMSO (MP Biomedicals ...
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bioRxiv - Cell Biology 2020Quote: ... HPSCs were plated at about 3.0×105 cells/cm in mTeSR1 or TeSR-E8 media (Stem Cell Technologies) supplemented with 2 μM Thiazovivin (Selleck Chemicals ...
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bioRxiv - Cell Biology 2020Quote: ... Naïve CD4 T cells were isolated from CB mononuclear cells (CBMCs) obtained using Lymphoprep (StemCell Technologies Inc., Canada), followed by a first step of negative selection by magnetic bead separation using EasySep Human Naïve CD4 T cell isolation kit (STEMCELL Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... B cells were enriched from PBMC by first using EasySep Human Pan-B cell Enrichment Kit (StemCell Technologies), and then stained with CD20-PB (2H7 ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from whole blood with the EasySepTM Direct Human NK Cell Isolation Kit (StemCell Technologies). The gating strategy for isolation of CD56+CD3- NK cells is shown in Figure S1 (Figure 2 – Supplement 1) ...
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bioRxiv - Developmental Biology 2021Quote: Dissociated AGM cells (either fresh or after explant culture) were added to 3.6ml methylcellulose (M3434; Stem cell technologies) and plated in triplicates (65.000 cells/plate) ...
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bioRxiv - Developmental Biology 2020Quote: Human embryonic stem cells (hESCs; H9 line) were cultured in mTeSR™1 basal medium (Stem Cell Technologies), in 6-well plates coated with matrigel (Corning Matrigel Matrix hESC-qualified) ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells and CD19+ B cells were negatively isolated using magnetic bead based separation (EasySep, StemCell Technologies). CD14+ monocytes were positively selected (EasySep ...
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bioRxiv - Genomics 2020Quote: KO cell lines generated using nucleofection of Cas9 RNP were detached with Accutase (Stem Cell Technologies, Vancouver, Canada) and stained with APC conjugated antibodies targeting B2M (2M2) ...
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bioRxiv - Developmental Biology 2019Quote: ... Single ES cells were seeded onto aggrewells in a 24 well plate (Stem Cell Technologies, Cat. No. 34411) at 1.5 × 10^6 cells per well in EB-medium ...
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bioRxiv - Genomics 2020Quote: ... B cells were purified from a B6 mouse using an EasySep B cell negative selection kit (StemCell Technologies) and labelled with CFSE before being transferred at 4e6 per mouse ...
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bioRxiv - Genetics 2020Quote: ... Red blood cell lysis was performed on ice using cold ammonium chloride (Stem Cell Technologies, cat. No 07800). Cells were subsequently stained as described in the panel (Table 1 ...
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bioRxiv - Bioengineering 2021Quote: ... and formed into 200 cell-sized spheroids overnight in inverse pyramidal PDMS microwells (AggreWell™, Stem Cell Technologies) treated with 0.5% Pluronic F-127 to prevent cell adhesion.
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bioRxiv - Genomics 2021Quote: ... Cells were plated onto six-well plates in media supplemented with 10 μM Y27632 compound (Stem Cell Technologies) for 24 hours ...
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bioRxiv - Cell Biology 2020Quote: T and B cell lineage depletion was performed using RosetteSep Human Hematopoietic Progenitor Cell Enrichment Cocktail (Stemcell Technologies) and Ficoll-Paque density gradient centrifugation from routine red cell exchange apheresis performed at Texas Children’s Hospital or Columbia University New York Presbyterian Hospital ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Pooled breast epithelial single cells from 15 patients were cultured in MammoCult media (Stem Cell Technologies, Vancouver, Canada) and treated with curcumin ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were harvested from SPLNs and MLNs using a CD4+ T cell isolation kit (STEMCELL technologies). Cells (2 × 105 cells/well ...
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bioRxiv - Cancer Biology 2022Quote: ... C57BL/6 CD8+ T cells were purified using the EasySep Mouse CD8 T Cell Isolation Kit (Stemcell technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T cells were purified by negative selection using the EasySep™ CD4 enrichment kit (Stem cell Technologies), and further enriched for memory cells by depletion of naïve T cells using CD45RA beads (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2022Quote: ... Isolated T cells were seeded in a fresh complete ImmunoCult™-XF T cell expansion medium (STEMCELL Technologies) at 1 × 106 cells/mL with 2 μL/mL ImmunoCult™ human CD3/CD28 T cell activator ...
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bioRxiv - Immunology 2020Quote: ... prior to CD4 and CD8 T cells isolation using EasySep Human T cell isolation kit (STEMCELL Technology, #17951). Isolated T cells were activated with Dynabeads Human T-Activator CD3/CD28 (ThermoFisher ...
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bioRxiv - Cancer Biology 2020Quote: ... The final clear cell pellets (containing mammary epithelial ducts) were dissociated into single cells using Accutase (StemCell Technologies). The obtained single cells were cultured using DMEM/F12 culture medium for IF experiments or used directly for flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... B cells were isolated from mouse spleens using EasySep mouse B cell isolation kit (Stemcell Technologies, Vancouver, Canada) according to manufacturer’s instruction ...
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Histone H3K36me2-specific methyltransferase ASH1L is required for the MLL-AF9-induced leukemogenesisbioRxiv - Cancer Biology 2021Quote: ... The red blood cells in the bone marrows were lysed by ammonium chloride solution (Stem Cell Technologies 07800) and filtered with a 70-μm nylon filter ...
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bioRxiv - Cancer Biology 2020Quote: ... The cell pellets were then washed twice in Ca2+ and Mg2+ free PBS (Stem Cell Technologies, Vancouver, Canada). The cell pellets were digested with a standard volume of suprapur nitric acid and hydrogen peroxide (Merck Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... cultures were initiated at a density of 500,000 cells/mL with serum-free StemSpan SFEM (Stem Cell Technologies) supplemented with Hydrocortisone (1µM) ...
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bioRxiv - Cell Biology 2019Quote: Approximately 1×106 hiPSCs were first detached in cell clumps using the gentle cell dissociation reagent (STEMCELL Technologies) and resuspended in cold PBS containing 50% of Geltrex and kept on ice ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... Bulk T cells were negatively selected using the EasySepTM Human T Cell Enrichment Kit (STEMCELL Technologies, Cambridge, MA), and 7.5 x 105 T cells were added per well to the MDC1 containing wells (MDC1 to T cell ratio = 1:10) ...
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bioRxiv - Biophysics 2020Quote: ... ImmunoCult™ Human CD3/CD28/CD2 T cell activator (short T-Ac) (Stem cell Technologies, Vancouver, BC, Canada) was added to the cell culture medium (25 μL per 1 mL of cell suspension) ...
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bioRxiv - Immunology 2020Quote: ... Naive B cells (CD19+CD27−IgD+) were enriched using Human naive B cell negative selection kit (Stemcell Technologies).
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bioRxiv - Genomics 2021Quote: ... progenitor BM sorted cells were seeded (250 or 500 cells/well) into 6 well SmartDish™ (STEMCELL Technologies). After incubation for 14 days ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells were purchased from Stemcell Technologies (#70025) and CD8+ T cells isolated using the EasySep Human CD8+ T Cell Isolation kit (Stemcell Technologies, #17953). Primary human CD8+ T cells were cultured for up to 24 days in ATCC-modified RPMI (Thermo Fisher ...