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Citations for Stemcell Technologies :
5401 - 5450 of 8825 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The diluted blood was layered on top of 15 mL Lymphoprep™ density gradient medium (StemCell Technologies Inc., Vancouver, Canada) in a 50 mL Falcon tube ...
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bioRxiv - Genomics 2022Quote: ... An addition 1.65 mL of homogenization buffer was topped up and mixed with 2.65 mL of gradient medium (5mM CaCl2, 50% Optiprep (Stemcell Technologies), 3mM Magnesium Acetate ...
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bioRxiv - Genomics 2022Quote: ... 4 mL of 29% Iodoxanol cushion was prepared with OptiPrep (Stemcell Technologies) and diluent medium (250 mM sucrose ...
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bioRxiv - Neuroscience 2022Quote: ... we placed a tissue “harp” on top of the organoid slice and applied several drops of recording medium (STEMCELL Technologies, #05793) around the organoid ...
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bioRxiv - Neuroscience 2022Quote: ... Cell plating: Cell plating medium consisted of 95 mL of BrainPhys Neuronal Medium (STEMCELL Technologies, Vancouver, Canada), 2 mL of iCell Neuronal Supplement B (FCDI) ...
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bioRxiv - Neuroscience 2022Quote: ... using the STEMdiff cerebral organoid kit (STEMCELL Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Single organoids were first transferred from maturation medium to ice-cold BrainPhys (STEMCELL Technologies) using cut 1000 μl pipette tips ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells (PBMCs) were isolated through Lymphoprep density gradient separation (Stemcell Technologies). Cells were used after cryopreservation ...
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bioRxiv - Biochemistry 2022Quote: ... plated in 0.1% gelatin (StemCell technologies) and cultured in KSR medium consisting of KnockOut DMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary human CD3+ T cells were isolated from PBMCs by negative selection using EasySep Human T Cell Isolation Kit (StemCell Technologies) or by positive selection using Dynabeads Human T-Expander CD3/CD28 (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... they were also isolated using the direct human neutrophil isolation kit (EasySep, StemCell Technologies) following manufactureŕs instructions.
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bioRxiv - Cancer Biology 2022Quote: ... CD3+ T cells were isolated from PBMCs using EasySep™ Human T Cell Isolation Kit (StemCell Technologies, Taiwan) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... initiation of the air-liquid interface was performed by removing media from the transwell insert and replacing the expansion media in the lower-chamber with ALI differentiation media (StemCell Technologies). At 48 hour intervals the transwell insert was rinsed with 500 μL PBS to prevent excessive mucous accumulation and the media was refreshed ...
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bioRxiv - Cell Biology 2022Quote: ... mouse intestinal organoids were cultured in 24-well plates using IntestiCult organoid growth medium (06005, STEMCELL Technologies). In short ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then passaged using GCDR and replated on 10-cm dishes coated with Animal Component Free (ACF) Attachment Substrate (StemCell Technologies); all subsequent passages were grown using the same medium and culture substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were split by dissociating to small clusters and replating at a 1:3-1:4 ratio using ReLeSR (StemCell Technologies) once cell clusters reached ∼70% coverage of the culture surface ...
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bioRxiv - Cell Biology 2022Quote: ... and then incubated for 30 min in Gentle Cell Dissociation Reagent (GCDR, StemCell Technologies). Tissue was then centrifuged and crypts were isolated by vigorous pipetting in ice-cold DMEM + 1% BSA followed by filtration through a sterile 70-μm cell strainer ...
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bioRxiv - Cell Biology 2022Quote: ... hiPSC were cultured in mTESR+ (StemCell Technologies) on culture surfaces coated with a thin film of hESC-qualified Matrigel (Corning) ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with Y-27632 (ROCK inhibitor) (10 μM, StemCell Technologies), penicillin-streptomycin 1% (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: Two different culture media were tested for hIO growth and differentiation: one commercial (“SCT-ODM”: IntestiCult Organoid Differentiation Medium, StemCell Technologies) and one homemade cocktail (“CCM” ...
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bioRxiv - Cell Biology 2022Quote: ... Domed crypts were grown in IntestiCult Organoid Growth Medium (StemCell Technologies) supplemented with Y-27632 (ROCK inhibitor ...
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bioRxiv - Cancer Biology 2022Quote: TS543 human glioma cell line was cultured in human NeuroCult™ NS-A Basal Medium (Stem Cell Technologies, cat #05751), supplemented with 10% NeuroCult™ Proliferation Supplement ...
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bioRxiv - Cancer Biology 2022Quote: ... human bFGF (10 ng/ml, Stem Cell Technologies, cat# 78003) and human EGF (20 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... and human EGF (20 ng/ml, Stem Cell Technologies, cat# 78006). HMC3 human microglia cell line is cultured in Eagle’s Minimum Essential Medium (EMEM ...
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bioRxiv - Cancer Biology 2022Quote: ... 3,000 cells per condition were seeded to methylcellulose based medium (catalogue no. 04034; STEMCELL Technologies) in duplicate ...
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bioRxiv - Cancer Biology 2022Quote: ... were seeded (70,000 cells each) in medium for long-term culture of human cells (catalogue no. 05150; STEMCELL Technologies) supplemented with hydrocortisone in collagen-coated plates ...
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bioRxiv - Cancer Biology 2022Quote: ... with 50ng/ml EGF (StemCell Technologies), 100nM IGF-1 (StemCell Technologies) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1x B27 (Themofisher Scientific) and 10 µM Y-27632(StemCell Technologies). The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... 100nM IGF-1 (StemCell Technologies), 1x N2 Supplement (Thermofisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were passage through shear stress with cold 1 U/ml Dispase/DMEM/F12 solution (StemCell Technologies) followed by Trypsin-EDTA (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... in mTeSR medium (StemCell Technologies, 85850) at a density of at 380,000 cells per well ...
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bioRxiv - Cell Biology 2022Quote: ... hPSC colonies were dissociated with Gentle Cell Dissociation Reagent (07174, StemCell Technologies) into small cell clusters and seeded on Matrigel or Culturex coated culture plates and cultured in mTeSR1 ...
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bioRxiv - Cell Biology 2022Quote: ... Clonal cells were cultured with mTeSR1 supplemented with 1x CloneR (StemCell Technologies) for 2 days ...
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bioRxiv - Cell Biology 2022Quote: ... the dissociated cells were resuspended in the transduction medium (1 uL to 5 uL of lentivirus, 4 to 8 ug/ml Polybrane[STR-1003-G, Sigma Aldrich], and 10 uM Y27632 [72304, StemCell Technologies] in mTeSR1), and centrifuged at 3200 xg for 30 to 90 min ...
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bioRxiv - Cell Biology 2022Quote: ... hPSCs were dissociated as single cells with Accutase (07922, StemCell Technologies; A6964-500ML, Sigma-Aldrich). Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304 ...
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bioRxiv - Cell Biology 2022Quote: ... Dissociated cells were plated on a Matrigel or Culturex coated culture plate and cultured in 10 uM Y27632 (72304, StemCell Technologies) containing mTeSR1 one day before differentiation ...
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bioRxiv - Cell Biology 2022Quote: ... were obtained from the CCHMC Pluripotent Stem Cell Facility (PSCF) (44) and cultured in feeder-free condition with mTeSR1 (85850, StemCell Technologies) and on a hESC-qualified Matrigel (354277 ...
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bioRxiv - Cell Biology 2022Quote: ... GINS precursors were dissociated by 5-10 min TrypLE treatment and aggregated (typically 2.0-2.4 million cells/well) in AggreWell400 (STEMCELL Technologies, 34450) using the manufacturer’s recommended protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse organoid culture: we used cryopreserved mouse intestinal organoids (70931, STEMCELL Technologies) derived from the C57BL/6 mouse small intestine ...
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bioRxiv - Cell Biology 2022Quote: ... carefully layered on top of 15 mL Lymphoprep™ density gradient medium (StemCell Technologies Inc., Vancouver, Canada), and centrifuged at 800 x g for 15 min (RT ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferrin (BIT, catalogue no. 09500; STEMCELL Technologies), 40 μg/ml low-density lipoprotein (catalogue no ...
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bioRxiv - Cancer Biology 2022Quote: ... heparin (2 μg/ml, Stem Cell Technologies, cat# 7980), human bFGF (10 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... 20% BIT 9500 (STEMCELL Technologies), 10ug/ml LDL (Low Density Lipoprotein ...
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bioRxiv - Cancer Biology 2022Quote: ... and Dispase (Stem Cell Technologies, 7913) + DNAse I (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Rehydrated tissue was then stained with Carmine Alum (Stem Cell Technologies, 07070) for 48-72 hours ...
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bioRxiv - Genomics 2022Quote: ... PBMC isolation was accomplished using SepMate tubes according to the manufacturer’s instructions with a few modifications (Stemcell Technologies, Cambridge, MA). Each 9 mL of blood was diluted in 15 mL of room temperature PBS and gently mixed for 15 min on a nutator ...
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bioRxiv - Genomics 2022Quote: ... 1x Gentle Collagenase/Hyaluronidase (Stem Cell Technologies, 07919), 1% penicillin/streptomycin ...
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bioRxiv - Genomics 2022Quote: Removal of red blood cells was done by treating cell pellets with cold ammonium chloride solution (Stem Cell Technologies, 07850) diluted with 1 part PBS-HF ...
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bioRxiv - Genomics 2022Quote: ... and 0.48 µg/mL Hydrocortisone (Stem Cell Technologies, 74144). After overnight digestion ...
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bioRxiv - Genomics 2022Quote: CD4 T-cells were isolated from 10-20 ml of peripheral blood using EasySep Direct Human CD4+ T cell Isolation kit (Stem Cell Technologies, cat #19662). The isolated CD4 T-cells were stored in Crystor CS10 (Stem Cell Technologies ...