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Citations for Macherey-Nagel GmbH :
401 - 450 of 557 citations for ssc mir 432 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... followed by agarose gel purification using a NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cancer Biology 2020Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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bioRxiv - Neuroscience 2022Quote: ... the vector backbone was gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Oligonucleotides pegRNA3-1 and pegRNA3-2 (Appendix Tables S1 and S2 ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Illumina sequencing libraries were prepared using the Hyperprep kit (KAPA ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 °C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Experimental success was evaluated by capillary electrophoresis (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The amplicons were cut out and purified with the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel). Finally ...
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bioRxiv - Physiology 2019Quote: ... and then purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel Milan, Italy). For each target gene ...
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bioRxiv - Biochemistry 2019Quote: ... followed by gel extraction and clean-up using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The products were mixed at an equimolar ratio and sent for paired-end sequening on Illumina HiSeq2000 to Novogene Bioinformatics Technology Co. ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragments were then purified with a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The integrity of the purified DNA fragments was confirmed by 2% (w/v ...
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bioRxiv - Plant Biology 2019Quote: ... for 30 min at 37°C and purified on PCR clean-up column (Macherey-Nagel, Düren, Germany). qPCR was performed with Kapa SYBR Fast qPCR master mix (Kapa Biosystems ...
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bioRxiv - Immunology 2020Quote: ... stained with ethidium bromide and purified with NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Germany). Purified PCR products were ligated in pGEM-T easy vectors (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... and cleaned using the NucleoSpin© Gel and PCR clean-up kit (Macherey-Nagel, cat. no. 740609) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... the vector backbone was gel-purified using the NucleoSpin Gel and PCR Clean‐up kit (Macherey-Nagel). Oligonucleotides pegRNA3-1 and pegRNA3-2 (Appendix Tables S1 and S2 ...
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bioRxiv - Molecular Biology 2022Quote: ... was digested with BsmBI and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Forward and reverse oligos containing the guide sequence of interest were phosphorylated and annealed and ligated into the parental plasmid with T4 PNK and T4 DNA ligase ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons (typically 2.5 mL) were purified using NucleoMag NGS Clean-up and Size Select (Macherey-Nagel) following manufacturer’s instructions and eluted in 450 μL distilled water ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The digested vector was purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel™, 740609) and then ligated with annealed gRNA oligos ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 °C) and fragments from the supernatant were purified by PCR clean-up spin column (Macherey-Nagel).
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bioRxiv - Cancer Biology 2023Quote: ... Gel bands were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, N. 740609.50) and sequenced by Sanger sequencing.
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bioRxiv - Genomics 2023Quote: ... and reverse-crosslinked DNA was subsequently purified using isopropanol and magnetic beads (Macherey-Nagel NucleoMag PCR Beads). Purified DNA was sheared to 300-500 bp with Covaris and subsequently size-selected by AMPure XP (Agencourt) ...
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bioRxiv - Plant Biology 2023Quote: ... fragments of interest were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), and sequenced with Eurofins SupremeRun reactions.
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bioRxiv - Biochemistry 2023Quote: ... Amplified DNA was purified using PCR purification product kit as described by the manufacturer (Macherey-Nagel, 740609.50). DNA amount was measured using a Nanodrop ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse cross-linked chromatin was purified using the NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... then extracted and purified by using the NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Purified DNA fragments were sequenced by the Biological Services Unit at the Weizmann Institute of Science ...
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bioRxiv - Developmental Biology 2019Quote: ... then extracted and purified by using the NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Purified DNA fragments were sequenced by the Biological Services Unit at the Weizmann Institute of Science ...
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bioRxiv - Microbiology 2019Quote: Amplicons were purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Gmbh & Co. KG, Düren, Germany). One-hundred ng of final DNA concentration were loaded into each well for DGGE and runs were performed on DGGE Electrophoresis System (C.B.S ...
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bioRxiv - Microbiology 2019Quote: ... purification of the high molecular weight product by gel extraction kit using PCR Clean-up kit (Macherey-Nagel), and subsequent ligation using T4 DNA ligase.
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bioRxiv - Cell Biology 2022Quote: ... The dsDNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... The eluted DNA samples were further purified with a NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL). The DNA libraries were prepared using a SMARTer ThruPLEX Tag-seq Kit (Takara Bio) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean‑up (Macherey-Nagel, Düren, Germany). Following digestion with restriction enzymes ...
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bioRxiv - Genetics 2020Quote: ... the single band was purified from a gel with a NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and its concentration determined ...
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bioRxiv - Synthetic Biology 2021Quote: Gel-purify the excised band using the Macherey-Nagel NucleoSpin Gel & PCR Clean-up kit (Macherey-Nagel, Germany). Insert the purified array into the opened plasmid backbone using In-Fusion cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2021Quote: ... DNA fragments were purified with a NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Restriction enzymes and T4 DNA ligase were obtained from Thermo Fisher Scientific and were used according to the supplier’s specifications ...
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bioRxiv - Microbiology 2022Quote: ... The products were inspected on agarose gel and purified using Nucleofast PCR purification plates (Macherey-Nagel, Dueren, Germa) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The DNA donors were then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and stored at -20°C or directly used for electroporation ...
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bioRxiv - Molecular Biology 2023Quote: ... and the reverse transcription products were purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). The reverse transcription products were eluted with 30 μL of HiDi formamide (Thermo Fisher) ...
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bioRxiv - Biochemistry 2022Quote: ... Table 1) followed by column purification using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). After sequencing the purified PCR product ...
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bioRxiv - Microbiology 2023Quote: ... and the backbone band was cut out and isolated using NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel). Backbone and insert were ligated using T4 Ligase (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting amplicons were pooled and gel purified using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Samples were sequenced on a MiSeq platform with ∼10 million reads per sample (Illumina) ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were purified via gel extraction using a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Illumina sequencing adapters were added with Phusion High-Fidelity DNA Polymerase (NEB ...
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bioRxiv - Immunology 2023Quote: ... purified again via agarose gel separation and using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany), before being pooled and submitted to GATC Biotech (Germany) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA bands of interest were isolated using the NucleoSpin® Gel & PCR Cleanup Kit from Macherey-Nagel. The extracted DNA underwent Sanger sequencing at Eurofins Scientific ...
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bioRxiv - Genetics 2023Quote: ... All probes were gel purified using the Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel, cat# 740609). The pixel intensities of the bands corresponding to linear ...
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using NTC buffer and the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The amplicons were purified using the NucleoSpin® Gel and PCR clean up kits from Macherey-Nagel (Bethlehem, PA), after which quantification and qualification of purified band(s ...
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bioRxiv - Biochemistry 2019Quote: ... purified according to the instructions of the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel GmbH, Düren, Germany) and cloned into the pET22b(+ ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitated DNA was purified with NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...