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Citations for Macherey-Nagel GmbH :
201 - 250 of 557 citations for ssc mir 432 5p RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... PCR products were visualized on a 1% TAE agarose gel and purified using the Nucleospin PCR Clean-up Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Three independent PCR reactions from the same library were combined after PCR and purified using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing was carried out on an Illumina HiSeq XTen at Macrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... All PCR or vector digestion products were purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50). The final plasmids were transformed into competent E.coli XL1-Blue ...
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bioRxiv - Plant Biology 2023Quote: ... RNA were extracted at different germination timepoints and in different tissues using the NucleoSpin® RNA Plant and Fungi Kit (Macherey Nagel, DuiJren, Germany) as described above but including a DNAse treatment (Macherey-Nagel, rDNAse set, DuiJren, Germany). RNA were quantified using a using a NanoDrop ND-1000 (NanoDrop Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... All amplified PCR products were isolated using silica membrane spin column technique (NucleoSpin® Gel, PCR clean up kit Macherey-Nagel, Germany) and were eluted in 20 µl of nuclease free water ...
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bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) products and plasmid digestion derivatives were purified using NucleoSpin® Gel and PCR Clean-up kits (Macherey-Nagel). Plasmids were extracted from E ...
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bioRxiv - Microbiology 2019Quote: ... All single fragment PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Overlap PCRs were carried out using 100 ng of each purified PCR products and the resulting fragment of interest was purified from agarose gel ...
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bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The overlap PCR product was purified from agarose gel and was directly used to transform wild type F ...
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bioRxiv - Plant Biology 2023Quote: ... The two resulting PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Ref. REF 740609.50; Macherey-Nagel, Düren, Germany), digested at 37°C overnight using FastDigest Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2023Quote: ... The dephosphorylated pEX18Tc vector and PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). TEDA reaction was then carried out by mixing 150 ng of pEX18Tc with the corresponding PCR products at a molar ratio of 1:4 ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were gel purified (Macherey-Nagel, USA) and used for ligation reaction ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products from genotyping were purified (Macherey-Nagel) and sequenced (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were purified with Nucleospin (Macherey-Nagel) as per manufacturer’s instructions and sequenced with Sanger sequencing (Eurofins or Genscript).
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bioRxiv - Microbiology 2021Quote: ... purified (gel and PCR clean-up kit, Macherey-Nagel) and subsequently sequenced by the use of primers oRH068 ...
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bioRxiv - Genetics 2022Quote: ... PCR products were isolated by gel extraction (Macherey-Nagel) and sequenced with a primer located upstream of the I-SceI cut site (DR-white2 ...
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bioRxiv - Immunology 2019Quote: ... purified (NuceloSpin Gel & PCR Clean-up Kit; Macherey-Nagel), and amplicons cloned into pCR4-TOPO (Invitrogen) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... PCR products were purified using NucleoSpin columns (Macherey-Nagel). The library vector pMB1-10x was prepared by restriction digestion with AarI (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) was used according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Genomics 2021Quote: ... Gel extraction is performed to isolate the PCR product with the correct size range using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). PacBio sequencing library preparation for both libraries was done by the Genomics Core Leuven (KU Leuven) ...
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bioRxiv - Microbiology 2023Quote: ... Read length was assessed by DNA agarose gel electrophoresis (1% w/v) and amplicons were purified from the PCR mix using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, DE) following manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified via gel purification or column purification using the NucleoSpin Gel and PCR Clean–up kit (Macherey-Nagel, Cat.#740609.50). Amplicon sequences were verified by Sanger sequencing using the Overnight Mix2Seq Kit service (Eurofins Genomics) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we purified the amplification reaction following the PCR clean-up protocol of the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Plant Biology 2022Quote: ... The PCR products were checked out in an agarose gel and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR fragments were extracted from agarose gels (Macherey-Nagel, 740609.250). pBlueRabbit vector was digested with EagI and XbaI ...
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bioRxiv - Immunology 2019Quote: ... using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel), and the DNA was then used for PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or by NucleoSpin Gel and PCR clean-up (Macherey-Nagel). PCR products were sequenced by Genewiz ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified using NucleoSpin® kit (Macherey-Nagel). Plasmids generated ...
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bioRxiv - Bioengineering 2022Quote: ... NucleoSpin Gel and PCR Clean-up Midi kit (MACHEREY-NAGEL) were used to extract the pooled PCR products from 24 samples in 200 μl of water ...
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bioRxiv - Microbiology 2020Quote: ... gel purified (Nucleoscopic gel and PCR cleanup kit; Macherey-Nagel), quality checked (BioAnalyzer HS DNA kit ...
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bioRxiv - Immunology 2019Quote: ... purified using a PCR purification kit (Macherey-Nagel Nucleospin; 740609), and sent for Illumina sequencing ...
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bioRxiv - Bioengineering 2021Quote: ... or NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). gRNA targeting BFP (gBFP ...
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bioRxiv - Immunology 2022Quote: ... PCR products were first purified using NucleoSpin kit (Macherey-Nagel) followed by Ampure bead purification (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using the “PCR Clean-up System” (Macherey-Nagel).
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bioRxiv - Neuroscience 2022Quote: ... concentrated using a PCR cleanup kit (Macherey-Nagel, Düren, Germany), and run on the Illumina NextSeq 500 sequencing platform (Illumina Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... using the PCR and gel clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel, Germany) were used for DNA clean-up from PCR products and agarose gel extracts ...
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bioRxiv - Cancer Biology 2023Quote: ... DNA was purified using the PCR Purification kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... and followed with a PCR clean-up (Macherey-Nagel, F40609.250). PCR products and plasmids were restricted with FastDigest BshTI (Thermo Fisher ...
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bioRxiv - Molecular Biology 2023Quote: ... purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), and cloned into pGEM-T Easy Vector (Promega) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were purified using NucleoSpin columns (Macherey-Nagel) and eluted into 33 μL water ...
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bioRxiv - Biophysics 2022Quote: ... and the 3’-terminal poly A) were amplified by PCR and purified using the NucleoSpin™ Gel and PCR Clean-up Kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... containing 1819 adenine bases) was amplified by PCR and purified using a NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) with Milli-Q water ...
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bioRxiv - Genetics 2021Quote: ... followed by purification (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Membranes were washed twice with 2x SSC ...
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bioRxiv - Bioengineering 2020Quote: ... NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) was used for DNA clean-up both from PCR products and agarose gel extracts ...