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Citations for Macherey-Nagel GmbH :
51 - 92 of 92 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... were grown overnight at 37 °C and plasmid was subsequently purified using the NucleoBond Xtra Midi kit (Macherey-Nagel). Plasmid DNA was solubilized in 150 µl of TE buffer and quantified using NanoDrop ND-1000 spectrophotometer (Celbio) ...
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bioRxiv - Microbiology 2022Quote: ... 4°C) and 100 µL of the supernatant was collected in gaschromatography (GC) glass vials (Macherey-Nagel, Düren, Germany); both were stored at -80°C ...
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 37°C and then cleaned up with the NucleoSpin RNA clean-up kit (Macherey-Nagel). RNA quality was verified on a 2100 Bioanalyzer Instrument (Agilent ...
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bioRxiv - Immunology 2021Quote: ... Virus was purified by centrifugation at 12,000 xg for 10 mins at 4 °C and genomic DNA extracted (NucleoSpin Tissue kit, Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Biophysics 2019Quote: ... centrifuged (60,000xg, 45min at 4°C) and the supernatant was incubated with Nickel agarose beads (Protino Ni-TED, Macherey-Nagel) for 1h at 4°C.
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bioRxiv - Cancer Biology 2022Quote: ... Collected pellets were stored at −80°C and genomic was DNA isolated using MN Nucleospin Tissue kit (Macherey-Nagel, Germany) according to manufacturers’ protocol ...
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bioRxiv - Biochemistry 2023Quote: ... of a saline solution (0.9%) and immediately stored at −20°C in 10 vol NucleoProtect RNA stabilization reagent (Macherey-Nagel) before further RNA extraction and target mRNA quantification.
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bioRxiv - Evolutionary Biology 2024Quote: ... for 45 min at 65°C and purified using NucleoBond AXG20 Mini columns according to the user manual (Macherey-Nagel). Final high molecular weight gDNA was quantified (Qubit) ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... Input and ChIP sample crosslinking were reversed at 65°C overnight in 50 μl TE + 200 mM NaCl + 0.1% SDS + 20-40 μg PK (Macherey-Nagel 740506). For sequencing ...
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bioRxiv - Microbiology 2021Quote: ... 107 cells were inoculated in 50 ml LB medium supplemented with 100 µg/ml spectinomycin and grown overnight at 37 °C for purification of the plasmid library DNA by miniprep (NucleoSpin Plasmid, Macherey-Nagel). The efficiency of fragment insertion (100% ...
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bioRxiv - Microbiology 2021Quote: ... selected clones were grown overnight at 37°C under agitation and the plasmid DNA was extracted and purified using the NucleoSpin plasmid Mini or Midi kit (Macherey-Nagel).
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bioRxiv - Genomics 2021Quote: ... genomic DNA was extracted from bacterial strains grown on King’s medium B (King et al. 1954) at 28°C for 24 h using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany). The quality of the genomic DNA was assessed by electrophoresis in 0.8% agarose gel.
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bioRxiv - Genomics 2019Quote: ... One colony per construct was grown overnight in a shake incubator at 37°C before plasmid extraction using the NucleoSpin Plasmid Transfection-grade kit (Macherey-Nagel). For each construct three biological replicates were performed by transfecting the plasmids into 80% confluent cells of MM001 in a 24 well plate ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
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bioRxiv - Plant Biology 2022Quote: ... The eluted desulfoglucosinolates were separated on a reversed phase C-18 column (Nucleodur Sphinx RP, 250 x 4.6 mm, 5µm, Macherey-Nagel, Düren, Germany) with a water (A)-acetonitrile (B ...
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bioRxiv - Molecular Biology 2023Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... All samples were immediately placed on dry ice and stored at −80 °C until DNA extraction using the NucleoSpin 96 soil kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... The soluble fraction was separated from the debris by centrifugation at 4000g for 40 minutes at 4°C and the supernatant was purified using 1 ml Ni-NTA columns (Macherey-Nagel). His-tagged UL12 was eluted using lysis buffer with increasing concentration of Imidazole (100 mM ...
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bioRxiv - Biochemistry 2023Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids were propagated in cultures of terrific broth medium with appropriate antibiotics overnight at 37 °C and purified with NucleoSpin miniprep kit (Macherey-Nagel) following the manufacturer’s prototol ...
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bioRxiv - Genomics 2023Quote: ... the DNA pellet was dried in the oven at 70°C for 30 min then re-suspended in 100 μl elution buffer BE (MACHEREY-NAGEL) (5 mM Tris solution ...
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bioRxiv - Genomics 2024Quote: ... Cross-linking was reversed by overnight incubation at 65°C and DNA fragments were purified using NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: ... and DMSO as a control at 28°C and 150 rpm using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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Pathogenic variants of sphingomyelin synthase SMS2 disrupt lipid landscapes in the secretory pathwaybioRxiv - Cell Biology 2022Quote: ... Reaction mixtures were incubated at 40°C for 4 h followed by 12 h incubation at 12°C and applied at 120 nl/s to NANO-ADAMANT HP-TLC plates (Macherey-Nagel, Germany) with a CAMAG Linomat 5 TLC sampler (CAMAG ...
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bioRxiv - Microbiology 2020Quote: We extracted DNA from the biomass pellets stored at −20°C using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... was added for 30 min at 37°C and DNA was purified using NucleoSpin Gel and PCR Clean-Up columns (Macherey-Nagel, #740609.250) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the samples were frozen and stored at −80 °C until the total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). The transcriptome was analyzed for three replicates from independent runs of the exposure experiment ...
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bioRxiv - Microbiology 2022Quote: ... The final stocks contained 12% glycerol as a cryo-protectant and were stored at -80 °C in crimp closed glass vials (Macherey-Nagel, ref. no. 70201HP and 70239).
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bioRxiv - Plant Biology 2021Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 100 μI of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). For each sample ...
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bioRxiv - Plant Biology 2022Quote: ... in pyridine followed by a 30 min treatment at 37 °C with 70 μl of N-methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co.KG ...
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bioRxiv - Plant Biology 2023Quote: ... in pyridine followed by a 30 min treatment at 37°C with 70 μl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA reagent; Macherey-Nagel, cat. no. 24589-78-4). An autosampler Gerstel Multi-Purpose system (Gerstel GmbH & Co ...