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Citations for Macherey-Nagel GmbH :
1 - 50 of 92 citations for SARS Coronavirus Nucleoprotein C Term E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... coli overnight culture (37°C, 130 rpm) via the NucleoBond Xtra Midi Kit (Macherey-Nagel) and precipitated by supplementing 0.1x volume 3 M sodium acetate (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... apical washes of Human coronavirus 229E (HCoV-229E) and MERS-CoV-infected hAECs were extracted using the NucleoMag VET (Macherey-Nagel) according to manufacturer’s guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Biochemistry 2022Quote: ... coli using NucleoBond Xtra Maxi columns (Macherey-Nagel) followed by isopropanol precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... coli was done using NucleoSpin Plasmid (MACHEREY-NAGEL). The mutations were verified by sequencing (Microsynth ...
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bioRxiv - Microbiology 2020Quote: ... coli using NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, Germany). Deletion of the degU ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cultures using NucleoBond Plasmid MAXI KIT (Macherey-Nagel) and resuspended in TE buffer ...
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bioRxiv - Microbiology 2022Quote: ... coli using the Nucleospin RNA Extraction Kit (Macherey-Nagel) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... coli clones using a Miniprep kit (Macherey-Nagel, 740588) and verified by single pass primer extension sequencing (ACGT Inc. ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH10 cells using a BAC prep kit (Macherey-Nagel). P19 Copg1-/- cells were transfected with 1µg BAC DNA using Effectene (QIAGEN) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli was performed using NucleoSpin Plasmid kit from Macherey-Nagel. DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures using a NucleoSpin Plasmid kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coli hosts containing DCFHP genes were maxi-prepped (Macherey-Nagel). HD-BIOP3 cells were then transfected with both components and underwent selection by glutamine deprivation.9 Resulting pooled cells were sorted and resulting single clones were analyzed as described throughout the manuscript ...
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bioRxiv - Microbiology 2022Quote: ... coli stock using the Nucleospin plasmid kit (Macherey-Nagel, Germany) and the PCR amplicon used was generated using C ...
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bioRxiv - Cancer Biology 2024Quote: ... coli using the Nucleobond Xtra Maxi EF Kit (Macherey-Nagel) and adequate representation of sgRNAs in the library was verified by next-generation sequencing and analysis with the python script count_spacers.py (Joung et al. ...
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bioRxiv - Neuroscience 2023Quote: ... coli transformed clones using the NucleoBond Xtra Midi Kit (Macherey-Nagel), according with the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... coli cultures using the NucleoSpin Plasmid kit (Macherey-Nagel, Cat.#740588.50). All plasmids contained ampicillin resistance (ampR ...
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bioRxiv - Microbiology 2022Quote: The yeast expression vector pAG416-GAL-SARS-CoV-2-3CL was freshly prepared by Midiprep (Macherey-Nagel), and variants were introduced by a modified single primer site-directed mutagenesis method (Shenoy and Visweswariah ...
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bioRxiv - Biochemistry 2022Quote: ... Coli using NuceloBond Xtra Maxi Kit per the manufacturers recommendations (Macherey-Nagel). All scFv-ACE2 ...
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bioRxiv - Immunology 2021Quote: ... coli cultures and purified using Nucleobond Xtra maxi EF columns (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... coli and purified using Protino Ni-IDA Resin (Macherey-Nagel, Düren, Germany). The EMSA experiments were conducted as described previously (Wu et al ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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bioRxiv - Genetics 2019Quote: ... coli BL21-Gold cells and purified on Ni-NTA Agarose (Macherey-Nagel). Two guinea pigs were immunized at Bioscience with His-DBN-1(1-132 ...
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Biochemistry 2020Quote: ... coli NM522 cells then extracted using a NucleoBond Xtra Midi kit (Macherey-Nagel). Quantification was performed using a SimpliNano spectrophotometer (Biochrom).
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bioRxiv - Microbiology 2020Quote: ... coli DH5α strains using the NucleoSpin® Plasmid DNA Purification kit (Macherey-Nagel). Cloning was performed using the appropriate restriction endonucleases and T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α cells for maxiprep using the NucleoBond Xtra Maxi kit (Macherey-Nagel). Linear DNA fragments used as expression templates in the cell-free reactions were PCR amplified using primers and templates listed in Sup Table 7 ...
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bioRxiv - Biochemistry 2023Quote: ... 4°C and incubated at 4 °C with Ni-NTA (Macherey-Nagel). The Ni-NTA column was washed with 10 column volumes of 20 mM HEPES/NaOH (pH 7.0) ...
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bioRxiv - Cancer Biology 2023Quote: ... coli under chloramphenicol selection and purified with the Endotoxin-Free MaxiPrep kit (Macherey-Nagel).
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bioRxiv - Neuroscience 2023Quote: ... coli bacterial cultures using NucleoSpin Plasmid QuickPure or NucleoBond Xtra MidiPrep kits (Macherey-Nagel, Düren, Germany) according to manufactureŕs instructions ...
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bioRxiv - Genetics 2021Quote: ... coli (High Efficiency) cells and subsequent plasmid isolation was performed using the NucleoSpin Plasmid kit (Macherey-Nagel).
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bioRxiv - Microbiology 2020Quote: ... The supernatant was applied onto a C-18 column (CHROMABOND C-18 ec, Macherey-Nagel, Düren, Germany) equilibrated with 5 mL 100% (v/v ...
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bioRxiv - Biochemistry 2024Quote: ... 4°C and the supernatant was incubated at 4 °C with 1.25 ml Ni-NTA agarose resin (Macherey-Nagel) for 1 h with agitation ...
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bioRxiv - Immunology 2022Quote: ... coli strain in presence of 100 μg/ml ampicillin and prepared by Midi DNA-prep kit (Macherey-Nagel, 740410) for the transient transfection ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: cDNA constructs for mammalian tissue culture were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (no. 740410.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were maintained in Escherichia coli and purified with a standard plasmid midi-prep kit (NucleoBond® Xtra Midi, MACHEREY-NAGEL, DE).
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified at 4 °C on Ni-NTA-Sepharose (Macherey-Nagel) packed in a gravity flow column ...
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Illumina sequencing libraries were prepared using the Hyperprep kit (KAPA ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 °C) and fragments from the supernatant purified with a Nucleospin PCR clean-up kit (Macherey-Nagel). Experimental success was evaluated by capillary electrophoresis (Agilent ...
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bioRxiv - Biochemistry 2019Quote: ... Glycopeptides were purified using a solid phase extraction on Chromabond C-18 sorbent (Macherey-Nagel, Düren, Germany). Samples were loaded onto beads in 0.1% TFA ...
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bioRxiv - Plant Biology 2019Quote: ... for 30 min at 37°C and purified on PCR clean-up column (Macherey-Nagel, Düren, Germany). qPCR was performed with Kapa SYBR Fast qPCR master mix (Kapa Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 4 °C) and fragments from the supernatant were purified by PCR clean-up spin column (Macherey-Nagel).
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bioRxiv - Cell Biology 2023Quote: ... lysed and stored at −80°C in 350 μl of lysis buffer (RA1, cat. 750961, Macherey-Nagel) every passaging step ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted DNA from fresh spores stored at -20°C using the Nucleospin Soil Kit (Macherey-Nagel, Germany). Genomes were sequenced using Illumina 150 bp paired-end sequencing technology at 46X coverage on average ...