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Citations for Macherey-Nagel GmbH :
101 - 150 of 485 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
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bioRxiv - Genomics 2021Quote: ... by loading 400 ng of each pooled sample on an agarose gel followed by a cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Immunology 2020Quote: ... and eGFP were amplified with the following primers and appropriate sized fragments were isolated from agarose gels with the PCR clean-up Gel extraction (Macherey-Nagel). PCR fragments were mixed and a consecutive PCR was done with the CD200R-F and GFP-R primers before digestion with PmlI and NotI and ligation into pMX ...
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bioRxiv - Developmental Biology 2021Quote: ... and the reverse primer 5’AACCCAGGTAATGAACACAGTTTCTAT. PCR products were run on a gel (supplemental Fig. 2B) and bands were gel purified (Macherey-Nagel), inserted in a pCR-bluntII-Topo vector (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplicon with the expected size was excised from the agarose gel and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). In order to induce a DNA double-strand break in close proximity to the site of insertion ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... Amplified libraries were cleaned up after gel electrophoresis (selected size between 200 bp to 500 bp) by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions for high percentage agarose gels.
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bioRxiv - Microbiology 2023Quote: ... Pooled libraries were concentrated by ethanol precipitation and purified by gel extraction of the corresponding library size using the NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Libraries were sequenced using the MiSeq Reagent Kit v3 with 300-bp paired end reads on a MiSeq (Illumina).
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bioRxiv - Systems Biology 2023Quote: ... The PCR products of correct size were purified by agarose gel electrophoresis (NucleoSpin Gel and PCR Clean-up Kit, Macherey-Nagel). The purified linear DNA was used for cloning of pTS040 by Gibson assembly (NEBuilder HiFi DNA Assembly Reaction ...
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bioRxiv - Microbiology 2023Quote: PCR product from multiple reactions was accumulated and gel extracted from a 0.8% (w/v) TAE agarose gel using a Nucleospin Gel and PCR clean-up kit (Macherey-Nagel, 740609.250). Gels were cut without exposing the DNA to UV radiation damage or stain ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... PCR products were confirmed on 2% agarose gels and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Purified PCR products were quantified using the Quant-iT dsDNA kit (Invitrogen ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 1% agarose gel and the correct band according to size was purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... pX458 plasmid was digested with BpiI (BbsI; Thermo, #ER1011) and gel-purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel); gRNA oligonucleotides were ligated into pX458 plasmid with T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2023Quote: ... and 16S amplicons (500 bp amplicon size) were purified by agarose gel separation using the NucleoSpin Gel and PCR Clean-up purification kit (Macherey-Nagel). Bacterial 16S amplifications were obtained using the FastStart High-Fidelity PCR system (Roche ...
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bioRxiv - Microbiology 2023Quote: ... DNA purification from agarose gel or enzymatic reactions was performed with the NucleoSpin Gel and PCR clean up kit (Macherey-Nagel). PCRs were performed with Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... RNA bands according to the size of the 18s and 28s rRNA were cut from the agarose gel and extraction was performed using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel). The extraction was performed according to the manufactureŕs protocol for RNA extractions from agarose gels using the buffer NTC (Macherey Nagel).
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Cell Biology 2020Quote: ... Purification of respective proteins from bacterial extracts was performed by affinity chromatography using Protino glutathione-conjugated agarose 4B (Macherey-Nagel, Düren, Germany). Except for GST-WAVE2-WCA ...
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bioRxiv - Genomics 2021Quote: ... embryos were ground in Tri-Reagent and RNA was purified using affinity chromatography (Nucleospin RNA Clean up kit, Macherey-Nagel, Duren, Germany). Potential contaminating DNA was removed by digestion with rDNase then RNA was purified using Beckman Coulter’s solid-phase reversible immobilization (SPRI ...
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bioRxiv - Neuroscience 2020Quote: ... The PCR products were gel purified (Macherey-Nagel, USA) and used for ligation reaction ...
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bioRxiv - Cell Biology 2021Quote: ... Fragments were gel purified (following kit instructions, Macherey-Nagel) and 30 ng of insert and 10 ng of vector ligated using 6U of T4 DNA ligase (Thermo ...
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bioRxiv - Microbiology 2021Quote: ... purified (gel and PCR clean-up kit, Macherey-Nagel) and subsequently sequenced by the use of primers oRH068 ...
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bioRxiv - Genetics 2022Quote: ... PCR products were isolated by gel extraction (Macherey-Nagel) and sequenced with a primer located upstream of the I-SceI cut site (DR-white2 ...
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bioRxiv - Immunology 2019Quote: ... purified (NuceloSpin Gel & PCR Clean-up Kit; Macherey-Nagel), and amplicons cloned into pCR4-TOPO (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were analysed using 1.5% agarose gel electrophoresis in 1x Tris-borate-EDTA (TBE) buffer and purified from the gel using a NucleoSpin® gel and PCR clean-up kit (Macherey-Nagel, Germany).
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bioRxiv - Immunology 2020Quote: ... The reactions were then run at 100V in a 1% agarose gel and the digest vectors were excised and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, USA) according to instructions set by the manufacturer ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cat#M0491S/L) and gel extractions were done with the Macherey-Nagel NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Cat#740609). DNA assemblies were carried out by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... The amplicons were resolved by agarose electrophoresis and extracted from the gel using NucleoSpin Gel and PCR Clean-up XS (Macherey-Nagel, Düren, Germany). The extracted DNA was then processed into libraries for sequencing using Ion Plus Fragment Library Kit and Ion Xpress Barcode Adapters 1-16 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmid DNA was isolated with NucleoSpin Plasmid Mini Kit and extracted from an agarose gel with NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel, Duren, Germany). All constructs were verified by restriction digest and Sanger sequencing ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified via gel purification or column purification using the NucleoSpin Gel and PCR Clean–up kit (Macherey-Nagel, Cat.#740609.50). Amplicon sequences were verified by Sanger sequencing using the Overnight Mix2Seq Kit service (Eurofins Genomics) ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Plant Biology 2022Quote: ... The PCR products were checked out in an agarose gel and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR fragments were extracted from agarose gels (Macherey-Nagel, 740609.250). pBlueRabbit vector was digested with EagI and XbaI ...
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bioRxiv - Immunology 2019Quote: ... using NucleoSpin Gel and PCR Clean-up kits (Macherey-Nagel), and the DNA was then used for PCR ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or by NucleoSpin Gel and PCR clean-up (Macherey-Nagel). PCR products were sequenced by Genewiz ...
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bioRxiv - Bioengineering 2022Quote: ... NucleoSpin Gel and PCR Clean-up Midi kit (MACHEREY-NAGEL) were used to extract the pooled PCR products from 24 samples in 200 μl of water ...
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bioRxiv - Bioengineering 2021Quote: ... or NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). gRNA targeting BFP (gBFP ...
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bioRxiv - Cell Biology 2023Quote: ... The large DNA fragment was gel purified (Macherey-Nagel, 740609.50) and used as a target backbone for cloning the AviTag constructs ...
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bioRxiv - Neuroscience 2023Quote: ... using the PCR and gel clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Gels were transferred to PVDF membranes (Macherey-Nagel, Düren, Germany) using a Transblot Turbo system (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel, Germany) were used for DNA clean-up from PCR products and agarose gel extracts ...
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bioRxiv - Microbiology 2023Quote: ... silica gel G60 plates (10 x 20 cm, Macherey-Nagel) were used to spot samples and lipids were separated with CHCl3/CH3OH (90:10 ...
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bioRxiv - Molecular Biology 2023Quote: ... purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel), and cloned into pGEM-T Easy Vector (Promega) ...