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Citations for Macherey-Nagel GmbH :
1 - 50 of 485 citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2022Quote: ... and the protein was affinity- purified from lysate using Ni-NTA affinity resin (Macherey-Nagel) and elution with imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... and purified by affinity column purification (Macherey-Nagel). Reverse transcription was performed using Maxima Reverse Transcriptase (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-IDA affinity beads (Macherey-Nagel, Bethlehem, PA, USA) were used to purify TBC-DEG assemblies ...
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bioRxiv - Microbiology 2020Quote: ... PomXK13AR15A-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Microbiology 2020Quote: ... PomXC-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Microbiology 2020Quote: ... PomXN-His6 was affinity purified with Protino Ni-NTA resin (Macherey-Nagel) from a batch ...
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... gel purified (Nucleoscopic gel and PCR cleanup kit; Macherey-Nagel), quality checked (BioAnalyzer HS DNA kit ...
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bioRxiv - Microbiology 2022Quote: ... The clarified lysate was subjected to affinity chromatography on Ni2+ agarose resin (Macherey-Nagel) and washed extensively with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... and isolated from the gel with the gel extraction kit (Macherey-Nagel). The amplicons were A-tailed and cloned with T/A cloning into a Golden Gate compatible version of pBluescript II SK(- ...
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bioRxiv - Plant Biology 2024Quote: ... gel-purified with the NucleoSpin Gel and PCR Cleanl1Jup kit (MACHEREY-NAGEL) and assembled using the In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Bioengineering 2024Quote: ... were gel purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) kit according to suppliers’ instructions ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and sent for Sanger sequencing.
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bioRxiv - Physiology 2023Quote: ... Products were run on 1% agarose gels and then gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... purified through agarose gel electrophoresis using NucleoSpin Gel and PCR Cleanup (Macherey-Nagel), eluted in water and concentrated SpeedVac Concentrator SAVANT SPD 121P (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Correct products were isolated with gel electrophoresis and gel extraction (Macherey-Nagel, F40609.250), and were ligated with T4 DNA ligase (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... followed by gel isolation with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: ... and analyzed and purified using an agarose gel with subsequent gel extraction with the “PCR clean-up and Gel extraction” Kit from Macherey-Nagel (Düren, Germany). A total of 90 ng of DNA per lane was incubated with different molar excesses of purified MalR protein (3-fold and 10-fold molar excess ...
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bioRxiv - Cell Biology 2021Quote: ... gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel), and cloned into the LentiORF pLEX-MCS Vector (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were gel-purified (NucleoSpin gel and PCR clean-up kit, Macherey-Nagel) and cloned into pCR-Blunt II-TOPO vectors using the Zero Blunt TOPO PCR Cloning Kit (Thermo) ...
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bioRxiv - Developmental Biology 2022Quote: ... Gel extractions were performed using NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel). Vectors were assembled via AQUA cloning technology (Beyer et al. ...
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bioRxiv - Bioengineering 2021Quote: ... PCR products were gel purified via NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and were Sanger sequenced ...
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bioRxiv - Plant Biology 2021Quote: ... isolated on agarose gel and purified (Macherey-Nagel NucleoSpin Gel and PCR Clean-up Kit). Transformants were generated using electroporation as previously described 61 ...
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bioRxiv - Cancer Biology 2021Quote: ... PCR amplicons were gel extracted using NucleoSpin Gel and PCR clean-up (Macherey-Nagel, Germany) kit according to manufacturer’s instructions and quantified using Nanodrop ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Nested PCR (position ...
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bioRxiv - Cell Biology 2022Quote: ... The proteins were then purified from bacterial extracts by affinity chromatography using glutathione-conjugated agarose (Macherey-Nagel), followed by cleavage of the GST-tag with PreScission protease ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified PCR products were gel-purified with a gel extraction kit (Macherey-Nagel GmbH, Dueren, Germany). The inhibitory unit (iUnit ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplified PCR products were gel-purified with a gel extraction kit (Macherey-Nagel GmbH, Dueren, Germany). Protocols using the pdMAX system (dx.doi.org/10.17504/protocols.io.binfkdbn ...
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bioRxiv - Microbiology 2021Quote: ... and purified from agarose gel using NucleoSpin® Gel and PCR clean up kit (MACHEREY-NAGEL). Point mutations were introduced into GBP sequences cloned in a pUC57 plasmid ...
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bioRxiv - Evolutionary Biology 2022Quote: ... visualized on 1% agarose gel and purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). Gibson assembly was performed with the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fragments were gel purified with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel). The 3×FLAG-tag (DYKDHDGDYKDHDIDYKDDDDK ...
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bioRxiv - Microbiology 2023Quote: 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... Both products were gel-purified using the NucleoSpin Gel and PCR clean-up system (Macherey-Nagel) and stored in 5 mM Tris-HCl pH 8.5 at-30°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... All PCR fragments were gel-purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL) by following manufacturer’s protocol ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... The linear fragments were purified on agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5 mM PMSF and purified by immobilized metal affinity chromatography using a 5 ml nickel column (Macherey-Nagel). The protein was eluted from the nickel column with solubilization buffer supplemented with 500 mM imidazole ...
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bioRxiv - Microbiology 2019Quote: ... followed by agarose gel purification using a NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Neuroscience 2022Quote: ... the vector backbone was gel-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Oligonucleotides pegRNA3-1 and pegRNA3-2 (Appendix Tables S1 and S2 ...
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bioRxiv - Biochemistry 2019Quote: ... followed by gel extraction and clean-up using the NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). The products were mixed at an equimolar ratio and sent for paired-end sequening on Illumina HiSeq2000 to Novogene Bioinformatics Technology Co. ...
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bioRxiv - Genomics 2020Quote: ... PCR products were gel-purified using a NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel 740609) followed by Sanger sequencing ...
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bioRxiv - Genomics 2022Quote: ... The PCR products were gel extracted using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Next ...
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bioRxiv - Neuroscience 2022Quote: ... the vector backbone was gel-purified using the NucleoSpin Gel and PCR Clean‐up kit (Macherey-Nagel). Oligonucleotides pegRNA3-1 and pegRNA3-2 (Appendix Tables S1 and S2 ...
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bioRxiv - Genomics 2023Quote: ... was purified from a 1% agarose gel using a Gel and PCR Clean-up Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and excised from a 0.8% agarose gel for purification using the NucleoSpin Gel Cleanup Kit (Macherey-Nagel). The insert pool and digested backbone were then ligated overnight at 16°C using T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Cancer Biology 2023Quote: ... Gel bands were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, N. 740609.50) and sequenced by Sanger sequencing.
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were gel purified with NucleoSpin gel and PCR clean-up kits (Macherey-Nagel, Germany) following the manufacturer’s protocol ...