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Citations for Macherey-Nagel GmbH :
51 - 100 of 153 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... coli (DE3) and affinity purified using Protino Ni-NTA Agarose (Macherey-Nagel). Subsequently ...
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bioRxiv - Genetics 2019Quote: ... coli BL21-Gold cells and purified on Ni-NTA Agarose (Macherey-Nagel). Two guinea pigs were immunized at Bioscience with His-DBN-1(1-132 ...
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bioRxiv - Biophysics 2022Quote: ... coli MC1061 cell line using the Nucleobond Xtra Maxi Kit (Macherey-Nagel). 1.3 μg DNA was used per 106 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Viral RNA was extracted from the cell culture supernatant using the NucleoSpin 96 Virus kit (Macherey-Nagel). Quantification of viral genomes was performed using the Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from cell culture supernatants using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and total RNA was extracted by using NucleoSpin Plus RNA virus Kit (Macherey-Nagel, cat no. 740984.10). For serum (50 μl) ...
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bioRxiv - Microbiology 2021Quote: Viral RNA was isolated from cell culture fluids using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Germany). Total RNA from cells was isolated using TRIreagent (Sigma ...
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bioRxiv - Microbiology 2022Quote: Viral RNA from the supernatant was extracted using the NucleoSpin RNA Virus Isolation Kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA of progeny virions was extracted from supernatants using the NucleoSpin RNA virus kit (Macherey-Nagel, #740956) according to the manufacturer’s instructions and stored at −80 °C until PCR-based analysis.
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bioRxiv - Biochemistry 2020Quote: ... coli NM522 cells then extracted using a NucleoBond Xtra Midi kit (Macherey-Nagel). Quantification was performed using a SimpliNano spectrophotometer (Biochrom).
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bioRxiv - Microbiology 2020Quote: ... coli DH5α strains using the NucleoSpin® Plasmid DNA Purification kit (Macherey-Nagel). Cloning was performed using the appropriate restriction endonucleases and T4 DNA ligase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α cells for maxiprep using the NucleoBond Xtra Maxi kit (Macherey-Nagel). Linear DNA fragments used as expression templates in the cell-free reactions were PCR amplified using primers and templates listed in Sup Table 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
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bioRxiv - Cancer Biology 2023Quote: ... coli under chloramphenicol selection and purified with the Endotoxin-Free MaxiPrep kit (Macherey-Nagel).
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bioRxiv - Systems Biology 2020Quote: The viral RNA from supernatant of infected cells was isolated using the NucleoSpin RNA virus isolation kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2021Quote: ... The vRNA in the cell culture supernatant was purified using a NucleoSpin® RNA virus kit (Macherey-Nagel, 740956) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Total cellular RNA from mock and virus-infected hAEC cultures was extracted with the NucleoMag RNA kit (Macherey-Nagel) according manufacturers guidelines on a Kingfisher Flex Purification system (Thermofisher) ...
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bioRxiv - Microbiology 2020Quote: RNAs from mock infected or infected (MOI=0.001) cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
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bioRxiv - Microbiology 2022Quote: RNAs from mock infected or infected (MOI=0.01) A549-hACE2 cell culture supernatant were extracted using the Nucleospin Dx Virus RNA purification kit (Macherey-Nagel). Then qRT-PCR was performed in triplicate as described20 ...
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bioRxiv - Cell Biology 2023Quote: ... coli overnight culture (37°C, 130 rpm) via the NucleoBond Xtra Midi Kit (Macherey-Nagel) and precipitated by supplementing 0.1x volume 3 M sodium acetate (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Neuroscience 2019Quote: RNA viral genomes were extracted from two Tripodi lab (EnvA/ SiR-CRE and EnvA/Sir-FLPo) and one Wickersham lab (RVΔG-4mCherry) rabies virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
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bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... The viruses were characterized by isolating viral RNA from 150-µL aliquots of the cell culture supernatant with the NucleoSpin RNA Virus kit (Macherey-Nagel) followed by conventional RT-PCR and bidirectional Sanger sequence analysis of the inserted SARS-CoV-2 sequences and flanking regions ...
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bioRxiv - Immunology 2024Quote: HIV RNA was extracted from plasma samples collected from either HIV-1 infected or uninfected humanized hNSG mice transplanted with CD4+ T cells from PWH in the absence or presence of immunotherapy using NucleospinTM RNA Virus kit (Cultek, Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... coli bacterial cultures using NucleoSpin Plasmid QuickPure or NucleoBond Xtra MidiPrep kits (Macherey-Nagel, Düren, Germany) according to manufactureŕs instructions ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL of the permeabilized sample was then processed using the Machery-Nagel NucleoSpin Virus kit (Macherey-Nagel, Catalog # 740983.50 and 740983.250).
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bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
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bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
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bioRxiv - Genetics 2021Quote: ... coli (High Efficiency) cells and subsequent plasmid isolation was performed using the NucleoSpin Plasmid kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2021Quote: ... and His-GST-Lap2α-CTD (100 μl) E.coli lysates were immobilized on 50 μl of Protino 4B glutathione agarose (Macherey-Nagel) equilibrated with binding buffer 1 (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2019Quote: ... Total protein was isolated using an RNA-protein extraction kit (Macherey-Nagel), as specified by the manufacturer ...
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bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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bioRxiv - Genetics 2022Quote: ... The protein concentration was determined by Protein Quantification Assay (Cat.# 740967,Macherey-Nagel). 10 µg of the protein was separated by SDS-PAGE electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein concentration was quantified using the Protein Quantification Assay kit (Macherey-Nagel). The protein samples were resolved using 4–15% Mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... All protein concentrations were determined with the MN Protein Quantification Assay (Macherey-Nagel). Western blotting was performed utilizing AnykD Mini-PROTEAN TGX Gels (Biorad ...
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bioRxiv - Immunology 2022Quote: ... coli strain in presence of 100 μg/ml ampicillin and prepared by Midi DNA-prep kit (Macherey-Nagel, 740410) for the transient transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein samples were extracted from the organs with the NucleoSpin RNA/Protein kit (Macherey-Nagel). Macherey-Nagel Bead Tubes Type F was used in tissue homogenization ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Biochemistry 2023Quote: cDNA constructs for mammalian tissue culture were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (no. 740410.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... and protein isolation kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... and protein extraction kit (Macherey-Nagel). RNA was further purified by using RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Genomics 2020Quote: Total protein was extracted from differentiated neurons at DIV 35 with the NucleoSpin RNA/Protein Kit (Macherey-Nagel 740933) according to manufacturer’s instructions ...