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Citations for Macherey-Nagel GmbH :
1 - 50 of 153 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 50 μg of His tag recombinant protein was incubated with 80μl Ni-NTA agarose beads (Macherey-Nagel) within gravity flow column (Agilent ...
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bioRxiv - Molecular Biology 2023Quote: The protein was purified under native conditions using the Protino Ni-NTA Agarose for His-tag protein purification Kit (Macherey-Nagel) with the gravity flow method ...
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bioRxiv - Plant Biology 2023Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Biochemistry 2021Quote: ... the His-tagged protein was immobilised to nickel beads (Macherey-Nagel), eluted with 300 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: The his-tagged protein was purified at 20 °C on Ni-NTA-Sepharose (Macherey-Nagel). The column was washed with 10 column volumes (CV ...
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bioRxiv - Molecular Biology 2019Quote: ... The clear solution was purified by column chromatography using a His-tag affinity matrix (Protino® Ni-NTA Agarose, Macherey-Nagel GMBH, Düren, Germany). The Vip3A protein was purified using a native protein purification protocol ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Molecular Biology 2020Quote: ... viral DNA was extracted from equal amounts of virus using a Nucleospin virus kit (MACHEREY-NAGEL, 740983.10). To account for potential differences in efficiency of DNA extraction ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Genomics 2022Quote: ... using the Nucleospin virus kit (Macherey-Nagel). Purified DNA was subsequently used for PCR amplification with the following primers ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoSpin Virus (Macherey-Nagel, Düren, Germany). DNA was quantified and the 260 nm/280 nm ratio was determined using a NanoDrop ND-1000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2023Quote: ... the NucleoSpin RNA Virus (Macherey-Nagel GmbH) or by semiautomated RNA extraction using the Easymag (BioMerieuxTM) ...
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bioRxiv - Microbiology 2022Quote: ... using the NucleoSpin RNA virus kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... or NucleoSpin 96 Virus (Macherey-Nagel, Duren, Germany) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2019Quote: The recombinant His-tagged AtDAT1 protein variants from this crude extract were purified with Protino Ni-NTA agarose from Macherey-Nagel, (Weilmünster Germany ...
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bioRxiv - Microbiology 2022Quote: ... NucleoSpin RNA virus kit (Macherey-Nagel; cat no. 740956.250) was used for RNA extraction.
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bioRxiv - Microbiology 2024Quote: ... by using the NucleoSpin RNA virus kit (Macherey-Nagel). For intracellular viral RNA cells were harvested in TRI Reagent and RNA extraction (Zymo Research ...
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bioRxiv - Microbiology 2022Quote: ... or NucleoSpin® RNA Virus (Macherey-Nagel GmbH, Düren, Germany). BHK-21 cells were infected with VSV-G-3CLpro-L wild type and F305L (3CLpro ...
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bioRxiv - Cell Biology 2023Quote: ... or from supernatants using NucleoSpin RNA virus kit (Macherey-Nagel). Reverse transcription and quantitative real-time PCR was performed using Luna Universal One-Step RT-qPCR Kit (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... coli using NucleoBond Xtra Maxi columns (Macherey-Nagel) followed by isopropanol precipitation ...
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bioRxiv - Neuroscience 2023Quote: ... coli was done using NucleoSpin Plasmid (MACHEREY-NAGEL). The mutations were verified by sequencing (Microsynth ...
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bioRxiv - Immunology 2019Quote: ... Virus RNA was extracted from supernatant using NucleoSpin RNA kit (Macherey-Nagel) and subsequently quantified quatified using qPCR with KAPA Fast Universal one-step qRT-PCR kit (KAPA ...
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bioRxiv - Microbiology 2023Quote: ... RNA was harvested using the NucleoSpin RNA Virus kit (MACHEREY-NAGEL, 740956) following manufacturer’s instructions and reverse-transcribed with SuperScript IV (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... extracellular viral RNA was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and intracellular viral RNA using the E.Z.N.A Total RNA Kit I (Omega Bio-tek) ...
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bioRxiv - Microbiology 2020Quote: ... coli using NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, Germany). Deletion of the degU ...
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bioRxiv - Molecular Biology 2020Quote: ... coli cultures using NucleoBond Plasmid MAXI KIT (Macherey-Nagel) and resuspended in TE buffer ...
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bioRxiv - Microbiology 2022Quote: ... coli using the Nucleospin RNA Extraction Kit (Macherey-Nagel) according to manufacturers’ instructions ...
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bioRxiv - Microbiology 2022Quote: ... coli clones using a Miniprep kit (Macherey-Nagel, 740588) and verified by single pass primer extension sequencing (ACGT Inc. ...
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bioRxiv - Molecular Biology 2022Quote: Viral RNA isolation was performed with the NucleoSpin RNA Virus kit (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... RNA extraction was performed using the NucleoSpin RNA Virus Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... coli DH10 cells using a BAC prep kit (Macherey-Nagel). P19 Copg1-/- cells were transfected with 1µg BAC DNA using Effectene (QIAGEN) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli was performed using NucleoSpin Plasmid kit from Macherey-Nagel. DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cultures using a NucleoSpin Plasmid kit (Macherey-Nagel, Germany), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... coli hosts containing DCFHP genes were maxi-prepped (Macherey-Nagel). HD-BIOP3 cells were then transfected with both components and underwent selection by glutamine deprivation.9 Resulting pooled cells were sorted and resulting single clones were analyzed as described throughout the manuscript ...
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bioRxiv - Microbiology 2022Quote: ... coli stock using the Nucleospin plasmid kit (Macherey-Nagel, Germany) and the PCR amplicon used was generated using C ...
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bioRxiv - Cancer Biology 2024Quote: ... coli using the Nucleobond Xtra Maxi EF Kit (Macherey-Nagel) and adequate representation of sgRNAs in the library was verified by next-generation sequencing and analysis with the python script count_spacers.py (Joung et al. ...
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bioRxiv - Microbiology 2021Quote: ... viral RNA was isolated with the NucleoSpin RNA Virus kit (Macherey-Nagel, Leiden, The Netherlands) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... −20M and GLT1cc infected human liver chimeric mice using the NucleoSpin Virus kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Viral RNA in supernatant cell culture was isolated with the Nucleospin Dx virus (Macherey-Nagel). Reverse transcription was performed with random primers and Superscript IV (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... coli transformed clones using the NucleoBond Xtra Midi Kit (Macherey-Nagel), according with the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... coli cultures using the NucleoSpin Plasmid kit (Macherey-Nagel, Cat.#740588.50). All plasmids contained ampicillin resistance (ampR ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA in each sample was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and quantified by qRT-PCR.
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from tissues using a NucleoSpin RNA Virus mini kit (Macherey-Nagel, 740956) at SCVL ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from 50µl aliquots of supernatant using the Nucleospin 96 virus kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... see Addgene #98034 for reference sequence) virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Coli using NuceloBond Xtra Maxi Kit per the manufacturers recommendations (Macherey-Nagel). All scFv-ACE2 ...
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bioRxiv - Immunology 2021Quote: ... coli cultures and purified using Nucleobond Xtra maxi EF columns (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... coli and purified using Protino Ni-IDA Resin (Macherey-Nagel, Düren, Germany). The EMSA experiments were conducted as described previously (Wu et al ...