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Citations for Macherey-Nagel GmbH :
2151 - 2200 of 2383 citations for Plant DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Digestion products were isolated on agarose gels and purified with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Plasmid isolation was performed with NucleoSpin Plasmid kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2023Quote: Dissected thyroids were immediately placed in RNAlater solution and total RNA was isolated using the NucleoSpin RNA Kit (Macherey-Nagel) according to the manufacturer’ s instructions ...
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bioRxiv - Bioengineering 2023Quote: We extracted RNA from live cells for bulk RNA-sequencing using the NucleoSpin totalRNA FFPE micro kit (Macherey-Nagel #740969.50) ...
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bioRxiv - Genomics 2022Quote: Each RNA sample was extracted from 2 retinae of a mouse using the NucleoSpin RNA Plus kit (Macherey-Nagel, PA). RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the cultured cells using the Nucleospin RNA Plus kit (cat. 740984, Macherey-Nagel, CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: cDNA constructs for mammalian tissue culture were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (no. 740410.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Immunology 2023Quote: Total RNA from BMDMs and RAW 264.7 cells was isolated using the NucleoSpin RNA II kit (Macherey-Nagel, Catalog #740955). The cDNA of pre-mRNA was synthesized by the use of random hexamer primers (Thermo-Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... RNA from FAC-sorted lung EC was extracted using the Nucleaospin® TriPrep Kit (MACHEREY-NAGEL, 740966.50, Düren, Germany Germany). RNA quantity and quality were assessed using the NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... The PCR products were purified using the NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, catalog no. 740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... We prepared a large amount of these plasmid vectors using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested in RIPA with 1X HALT or the RNA lysis buffer from the Nucleospin RNA kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the PCRs were purified with the NucleoSpin Gel and PCR Clean-up XS kit (#740611.50, Macherey-Nagel) for long-read amplicon sequencing using Oxford Nanopore Technology (via Plasmidsaurus).
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bioRxiv - Cell Biology 2024Quote: ... Products from four PCR reactions were pooled and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel #740609). Equal amounts of products were mixed and sequenced on Illumina NextSeq 500 instruments using the NextSeq high-output kit (75 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
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bioRxiv - Plant Biology 2024Quote: ... and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel). All amplicons obtained from a single biological replicate were pooled in equimolar amounts and a total of 100 ng were used for library preparation following the modified Illumina Nextera Flex library preparation protocol described before (28 ...
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in RNA lysis buffer and RNA was isolated using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel). For some experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were separated on agarose gels and PCR fragments isolated using a Nucleo-Spin Gel and PCR Clean-up kit (Macherey-Nagel). Sanger sequencing of PMEL and KIT ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The products were isolated by cutting the band from an agarose gel and clean-up with the NucleoSpin Extract II kit (Macherey-Nagel). Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Large quantities of plasmid DNAs for transfection were prepared from the pellets of overnight cultures of pNC-HN8 and pNC-HN8GGm clones using the NucleoBond Xtra Midi kit (Macherey-Nagel) as instructed by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... Contaminating genomic DNA was removed using DNase and the total RNA sample was further purified with a NucleoSpin RNA Clean-up kit (Macherey-Nagel). Sequencing was then performed on an Illumina HiSeq 1500 instrument as 101 bp single read runs.
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bioRxiv - Bioengineering 2021Quote: Cell pellets were washed and lysed using RA1 RNA extraction buffer and purified using the NucleoSpin RNA kit (MAcherey-Nagel; 740955.250) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
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bioRxiv - Genomics 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin-RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were collected into the RA1 lysis buffer and extracted using the Nucleospin RNA Plus Extraction kit (740984, Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... the band corresponding to the amplicon was excised from the gel and purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was extracted by phenol-chloroform and cleaned up using the NucleoSpin® RNA XS extraction kit (Macherey-Nagel, Germany). Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA (containing viral genomic RNA) was extracted 48 h after transfection using NucleoSpin RNA Plus kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
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bioRxiv - Immunology 2021Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products with multiple bands were excised and purified from gel using the Nucleospin Gel and PCR Clean-up kit from Macherey-Nagel, or cloned using the NEB® PCR cloning kit (New Englands BioLabs ...
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bioRxiv - Molecular Biology 2019Quote: ... Twenty microliters have been collected for protein analysis and RNA was extracted from remaining supernatant with the Nucleospin RNA extraction II Kit (Macherey-Nagel).
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bioRxiv - Neuroscience 2019Quote: RNA viral genomes were extracted from two Tripodi lab (EnvA/ SiR-CRE and EnvA/Sir-FLPo) and one Wickersham lab (RVΔG-4mCherry) rabies virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
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bioRxiv - Synthetic Biology 2021Quote: ... the vector pEHLYA5 in the band corresponding to 3612 bp was cleaned with Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Plasmids pCANTAB6_H107Y ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were extracted from yeast cells disrupted by bead beating and purified using the Nucleospin RNA II kit (Macherey-Nagel). For RNA-seq analysis ...
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bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
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bioRxiv - Genomics 2021Quote: ... by loading 400 ng of each pooled sample on an agarose gel followed by a cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs ...