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Citations for Macherey-Nagel GmbH :
2051 - 2100 of 2234 citations for Plant DNA Purification kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and RNA was extracted by phenol-chloroform and cleaned up using the NucleoSpin® RNA XS extraction kit (Macherey-Nagel, Germany). Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were excised from 2% agarose gels and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) using NTC buffer for solubilization of the gel slices ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: Viral RNA was isolated from 4 µl of a typical lentivirus preparation (∼2×106 TU/µl) using the NucleoSpin RNA Virus mini kit (Macherey-Nagel). Purified RNA was used as a template for reverse transcription using the SuperScript VILO cDNA Synthesis Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA (containing viral genomic RNA) was extracted 48 h after transfection using NucleoSpin RNA Plus kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
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bioRxiv - Immunology 2021Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products with multiple bands were excised and purified from gel using the Nucleospin Gel and PCR Clean-up kit from Macherey-Nagel, or cloned using the NEB® PCR cloning kit (New Englands BioLabs ...
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bioRxiv - Molecular Biology 2019Quote: ... Twenty microliters have been collected for protein analysis and RNA was extracted from remaining supernatant with the Nucleospin RNA extraction II Kit (Macherey-Nagel).
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bioRxiv - Neuroscience 2019Quote: RNA viral genomes were extracted from two Tripodi lab (EnvA/ SiR-CRE and EnvA/Sir-FLPo) and one Wickersham lab (RVΔG-4mCherry) rabies virus samples using a Nucleospin RNA kit (Macherey-Nagel, Germany) and treated with DNase I (37°C for 1 hour ...
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bioRxiv - Synthetic Biology 2021Quote: ... the vector pEHLYA5 in the band corresponding to 3612 bp was cleaned with Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Plasmids pCANTAB6_H107Y ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNAs were extracted from yeast cells disrupted by bead beating and purified using the Nucleospin RNA II kit (Macherey-Nagel). For RNA-seq analysis ...
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bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
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bioRxiv - Genomics 2021Quote: ... by loading 400 ng of each pooled sample on an agarose gel followed by a cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Genomics 2019Quote: ... DNA was extracted from fish fin clips for the ddRADseq and muscle tissue for the epiRADseq libraries using the NucleoSpin Tissue kit (Macherey-Nagel) following the manufacturers recommendations ...
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bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and a ∼500 bp portion around the predicted CRISPR cut site was amplified by PCR and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). This fragment was then ligated into an EGFP-N vector using Xho1 and Apa1 restriction sites and transformed into DH5-a competent cells ...
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bioRxiv - Neuroscience 2021Quote: ... The resulting SORL1-202 amplicons were spin-column purified using a Nucleospin Gel and PCR Clean-up Kit (#740609, Macherey-Nagel) prior to validation by DNA sequencing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from 30 mg of lice (15 lice) using the Total RNA isolation Nucleospin RNA kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Synchronized ring stage parasites were transfected with 100 – 200 μg plasmids purified by NucleoBond Xtra Midi EF midiprep kit (Macherey-Nagel) by electroporation using Bio-Rad laboratories gene pulser X cell31 ...
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bioRxiv - Cell Biology 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from flow-sorted naive CD8+ T cells (n = 300 per condition) using a NucleoSpin RNA XS Kit (Macherey-Nagel), and cDNA was synthesized using Reverse Transcription Master Mix (Fluidigm) ...
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bioRxiv - Immunology 2021Quote: ... identical conditions were used except DMEM was used instead of HCM media and RNA extractions were performed using only the NucleoSpin RNA kit (Macherey-Nagel). Where ruxolitinib treatment is indicated ...
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bioRxiv - Cell Biology 2021Quote: Six replicate RNA preparations for each genotype or condition were prepared using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, #740984.50) kit ...
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bioRxiv - Genomics 2019Quote: ... One colony per construct was grown overnight in a shake incubator at 37°C before plasmid extraction using the NucleoSpin Plasmid Transfection-grade kit (Macherey-Nagel). For each construct three biological replicates were performed by transfecting the plasmids into 80% confluent cells of MM001 in a 24 well plate ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from U251 cells (SETD6 control vs. KO or Empty vs. TWIST1 WT) using the NucleoSpin RNA Kit (Macherey-Nagel). Samples were prepared in triplicates (SETD6 KO ...
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bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from snap frozen mouse or human liver tissue using NucleoSpin RNA Mini kit (Macherey-Nagel, Düren, Germany). First strand cDNA was synthesized from 1 μg total RNA using the QuantiTect Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2022Quote: ... The amplicon with the expected size was excised from the agarose gel and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). In order to induce a DNA double-strand break in close proximity to the site of insertion ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from three independent experiments of the mock- and KSHV-infected lymphatic ECFCs isolated as an adherent subpopulation from donor 1 using the NucleoSpin RNA extraction kit (Macherey-Nagel), after which purity and concentration was determined with NanoDrop spectrophotometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA of five corrected hiPSC clones and the naïve parental iPSC line was isolated using the Macherey-Nagel NucleoSpin RNA mini kit (Macherey-Nagel, 740955) according to manufacturer’ s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... at least eight separate tubes were used for PCR and then pooled and purified by using PCR and gel extraction kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The BAG6 target region was amplified from the genomic DNA from all samples using Q5 High-Fidelity Master Mix and subsequently purified using a NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. The purified samples were sent for sanger sequencing and the proportion of BAG6 knockout cells in each sample was estimated using the ICE CRISPR analysis tool (https://www.synthego.com/products/bioinformatics/crispr-analysis).
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from control and IMCD PCM1 KO cells before SAG treatment and 24 h after SAG treatment using NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and sent for Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Amplified libraries were cleaned up after gel electrophoresis (selected size between 200 bp to 500 bp) by a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions for high percentage agarose gels.
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bioRxiv - Microbiology 2023Quote: ... Pooled libraries were concentrated by ethanol precipitation and purified by gel extraction of the corresponding library size using the NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Libraries were sequenced using the MiSeq Reagent Kit v3 with 300-bp paired end reads on a MiSeq (Illumina).
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted from snap frozen tissue samples in RNAlater with the use of NucleoSpin kit according to the manufacturer’s instructions (NucleoSpin® RNA, Macherey-Nagel) and measured by NanoPhotometer®N60 (Implen) ...
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bioRxiv - Molecular Biology 2023Quote: ... positive colonies were cultured in 100 ml of 2YT medium and prepped using a midi-scale commercial kit (Macherey-Nagel 740410100).
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated from confluent cells from one well of a 6-well plate with the NucleoSpin RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The PCR products of correct size were purified by agarose gel electrophoresis (NucleoSpin Gel and PCR Clean-up Kit, Macherey-Nagel). The purified linear DNA was used for cloning of pTS040 by Gibson assembly (NEBuilder HiFi DNA Assembly Reaction ...