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Citations for Macherey-Nagel GmbH :
2151 - 2200 of 2219 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were maintained in Escherichia coli and purified with a standard plasmid midi-prep kit (NucleoBond® Xtra Midi, MACHEREY-NAGEL, DE).
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from fresh mycelium and conidia suspension after single-ascospore isolation and growth for five days on MEA media using the Nucleospin soil kit (Macherey-Nagel, Düren, Germany). For checking that isolated spores were recombinant offspring and not asexual conidia ...
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bioRxiv - Evolutionary Biology 2024Quote: We isolated total genomic DNA from silica-dried or herbarium leaf tissue using the NucleoSpin Plant II kit: Genomic DNA from plants (Macherey-Nagel, Düren, Germany) following a modified version of the manufacturer’s manual (Supplementary Data Table S4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we purified the amplification reaction following the PCR clean-up protocol of the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The constructs were assembled into the AAV2-ITR plasmid via molecular cloning and purified using the NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Düren, Germany). The purified plasmids were sent to Vigene Biosciences (now Charles River ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA extraction was performed on dried tissue that has been preserved in silica gel for several years using the Nucleospin R 96 plant kit II (Macherey-Nagel, Düren, Germany), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... then genomic DNA was isolated using the Sodium Dodecyl Sulfate (SDS) procedure of the NucleoSpin Plant II DNA isolation kit (Macherey-Nagel, Dueren, Germany). Preparation of DNA libraries for bisulfite sequencing was performed as described in (Nunn et al. ...
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bioRxiv - Microbiology 2022Quote: ... Each female was held above a sterile microcentrifuge tube filled with 700 μl lysis buffer SL1 (NucleoSpin Soil kit, Macherey-Nagel, Düren, Germany), and the ovipositor was dipped into the liquid and moved in circles for one min to wash microbes off of the ovipositor’s surface (WS) ...
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bioRxiv - Microbiology 2023Quote: ... Read length was assessed by DNA agarose gel electrophoresis (1% w/v) and amplicons were purified from the PCR mix using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, DE) following manufacturer’s instructions.
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bioRxiv - Bioengineering 2022Quote: ... 8×104 661W cells were seeded in a 24-well culture plate with DMEM and transfected with the plasmids containing guides using Lipofectamine™ Cells were harvested 72 hours following the transfection and Genomic DNA was then extracted using NucleoSpin® Tissue kit (Macherey-Nagel) following the manufacturer’s recommendation.
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bioRxiv - Bioengineering 2022Quote: Cultured cells were lysed in Macherey-Nagel RA1 buffer containing 1% β-mercaptoethanol and total RNAs were isolated using NucleoSpin RNA isolation kit (Macherey-Nagel 740955). Reverse transcription and PCR amplification were performed as described before21 ...
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bioRxiv - Plant Biology 2023Quote: ... and the resultant powder was transferred and redissolved in lysis buffer provided by the Nucleospin Plant II kit (Macherey-Nagel, Clonetech, CA, USA). Genome isolation was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µg of ground mycelium was used for extraction of high molecular weight DNA with the NucleoBond HMW-Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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ADEVO: Proof-of-concept of Adenovirus Directed EVOlution by random peptide display on the fiber knobbioRxiv - Bioengineering 2023Quote: ... infected cells were washed five times to eliminate non-internalized VPs and DNA was extracted using the NucleoSpin Tissue kit (Macherey-Nagel #740952-250). Internalized vector genomes were titrated by qPCR using a forward primer in the U exon ...
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bioRxiv - Synthetic Biology 2023Quote: ... PCR amplicons were purified via gel purification or column purification using the NucleoSpin Gel and PCR Clean–up kit (Macherey-Nagel, Cat.#740609.50). Amplicon sequences were verified by Sanger sequencing using the Overnight Mix2Seq Kit service (Eurofins Genomics) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA were isolated for each brain area or lung tissues from the selected animal tissues using the NucleoSpin TriPrep kit (Cat# 740966.50, Macherey-Nagel GmbH & Co.KG, Germany), following the manufacturer’s guidelines.
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bioRxiv - Microbiology 2023Quote: ... If no genomic DNA could be obtained strains were cultured according to the manufacturer’s protocol and DNA was extracted using the NucleosSpin Plasmid (NoLid) Mini kit (Macherey-Nagel cat. No. 740499) with vortexing after addition of BufferA2 and BufferA3 ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 50 mg of frozen and fine ground rosette leaf material from four biological replicates per genotype and growth condition using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from the interphase of extracted cells as described before [20] by using the NucleoSpin® RNA kit (MACHEREY-NAGEL, 740955.50). Isolated RNA was converted to cDNA by using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™ ...
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bioRxiv - Genomics 2024Quote: The RNA from 3 independent replicates (100mg/replicate) per regime were extracted using the Macherey-Nagel Mini kit for RNA purification (Macherey-Nagel, Allentown, PA). Sample purity was assessed using the NanoDrop ND-1000 Spectrophotometer (NanoDrop ...
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Chromosome-level assembly of Cucumis sativus cv. ‘Tokiwa’ as a reference genome of Japanese cucumberbioRxiv - Genomics 2024Quote: We also extracted RNA from the leaves for gene annotation used by NucleoSpin RNA Plant kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany).
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bioRxiv - Genomics 2020Quote: ... and small (<200 nt) and large RNA (>200 nt) were extracted in separate fractions using the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany) following the manufacturer’s recommended protocols ...
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bioRxiv - Plant Biology 2019Quote: ... genomic DNA from ‘Bartlett.DH’ was purified from young rolled leaves and young meristem tissue using the NucleoSpin Plant II DNA extraction kit (Macherey-Nagel GmbH, Düren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A sample of 100 mg of the ground powder (or as much as was available) was subject to DNA extraction using a NucleoSpin™ DNA Stool Kit (Macherey-Nagel, Oensingen, Switzerland). We found only one sample of overwintered leaves hanging in a tree ...
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bioRxiv - Neuroscience 2020Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissue using a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers suggested by the stock facility (forward ...
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bioRxiv - Systems Biology 2020Quote: ... with ALLinTM HiFi DNA Polymerase (HighQu, Kraichtal, Germany) and purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Plasmids were constructed from PCR-generated fragments and pET16b vector cut with restriction enzymes using the isothermal DNA assembly method (48) ...
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bioRxiv - Microbiology 2020Quote: ... The final PCR product from the 5’ RACE procedure was excised from a 1% agarose gel and purified by column capture (Macherey-Nagel Nucleospin mini kit). The purified product was then Sanger sequenced (Eurofins Scientific ...
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bioRxiv - Genetics 2019Quote: ... purpureus specimens from RPPN Pasmado Conservation Unit were selected and a small stem slice from each individual were collected to DNA extraction using NucleoSpin® Plant II DNA extraction kit (Macherey-Nagel, Düren, Germany). Based on the five defined criteria for primer design ...
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bioRxiv - Microbiology 2021Quote: ... The DNA product was verified by gel electrophoresis and purified with the NuceloSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. No. 740609.50). The concentration of the purified PCR product was adjusted to 5 ng/μl in 15 μl and send to Sanger sequencing by Eurofins Genomics ...
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bioRxiv - Neuroscience 2021Quote: ... Genotyping for the tm1a cassette was performed using gene-specific polymerase chain reaction (PCR) on DNA extracted from ear tissues with a tissue DNA extraction kit (Macherey-Nagel, Bethlehem, PA, USA) with primers (forward 5’ to 3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was extracted from 100 freshly harvested seeds in three biological replicates using the NucleoSpin® RNA Plant and Fungi kit (Macherey-Nagel, Düren, Germany), according to the manufacturer instructions ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and small (<200 nucleotides) and large (>200 nucleotides) RNA were extracted separately for each tissue with the NucleoSpin® miRNA kit (Macherey-Nagel; Düren, Germany). RNA quality and quantity were determined using a NanoDrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from tobacco leaf disks sampled from T1 plants and quickly frozen in liquid nitrogen using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized using 1 µg of total RNA according to the protocol in the RevertAid Reverse Transcriptase kit (Fermentas ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from ACE-grown cells (DSMZ medium 461 with 5 mM ACE) with the NucleoSpin® Microbial DNA kit (Macherey-Nagel, Düren, Germany) according to the manual and using wide-bore tips ...
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Cell Biology 2024Quote: ... RNAlater was removed then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenised with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Genetics 2024Quote: RNA was extracted from a total of 24 samples of male brain (four vgll3*EE and four vgll3*LL individuals for each of the three time points) using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The collected samples were transferred to tubes containing 1.4 mm ceramic beads from Omni International ...
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bioRxiv - Microbiology 2023Quote: ... inoculated seeds and seedlings of the SynCom conditions (control, 6-strain, 8-strain, 12-strain SynComs) with the NucleoSpin® 96 Food kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... a 450 bp long sequence upstream of timA was synthesized by PCR from EGD-e genomic DNA using primers TE74 and TE75 and purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany). For the EMSA experiment ...
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bioRxiv - Synthetic Biology 2023Quote: ... Base-editing efficiency when targeting a plasmid-borne gene was estimated by isolating plasmid DNA upon 24-h editing treatment by using the NucleoSpin™ plasmid EasyPure kit (Macherey-Nagel, Dueren, Germany). Either the isolated plasmids or the purified PCR products from independent samples were Sanger-sequenced using the Mix2Seq™ kit (Eurofins Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Systems Biology 2023Quote: ... The pellet was washed once with sterile H2O and then each library was purified over two columns of the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany). A total of 17 Mio were harvested ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extractions were either performed with a modified CTAB 2%/PVPP 2% protocol (see Jolly et al. 2003) or the NucleoSpin®Tissue 96 kit (Macherey-Nagel, Karlsruhe, Germany).
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bioRxiv - Plant Biology 2024Quote: ... Maize leaf discs sampled at 4 dpi were homogenized using metal beads in a Retsch mixer mill and fungal genomic DNA was extracted with NucleoSpin® Plant II Kit (Macherey-Nagel, Dueren, Germany) as explained before ...
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bioRxiv - Genomics 2024Quote: ... tissues were immediately frozen in liquid nitrogen and stored at -80°C prior to total RNA isolation with NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel Inc., PA, US). RNA libraries for RNA-Seq were prepared using the Illumina TruSeq Stranded Total RNA Ribo-Zero Plant kit (Illumina ...
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bioRxiv - Genetics 2024Quote: ... using Ion Xpress™ Plus Fragment Library Kit (Thermo Scientifc, USA) and NucleoMag® NGS Clean-up and Size Select beads (Macherey-Nagel, Germany) following manufacturers’ instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and bands of the correct size were excised and cleaned up with a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Cat. No. 740609.5) followed by cleanup with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Genetics 2024Quote: ... PCRs for loci of interest were run using Amplicon-EZ (Genewiz) partial IlluminaÒ adapters and amplicons were processed using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Catalog no. 740609.250). Paired end (2 x 250 bp ...