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Citations for Macherey-Nagel GmbH :
2101 - 2150 of 2219 citations for Alpaca ZAP Antibody Internalization Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... and the resulting RNA pellet was further purified and DNAse treated using the Nucleospin RNA Clean-up Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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Chromosome-level assembly of Cucumis sativus cv. ‘Tokiwa’ as a reference genome of Japanese cucumberbioRxiv - Genomics 2024Quote: ... We extracted genomic DNA from 3 g of young leaves using Nucleobond HMW DNA kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). We basically followed the DNA extraction protocol provided by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted in duplicate from ∼1 g of young leaf tissue using the Nucleobond HMW DNA Extraction kit (Macherey-Nagel, Germany). HMW DNA purity and concentration was assessed by Nanodrop and Qubit ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to pellet the cell debris and the RNA was extracted from the supernatant using the two column-system of the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany). DNA removal was ensured by the on-column DNA digestion according to the manufacturer’s description ...
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bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
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bioRxiv - Microbiology 2019Quote: Environmental DNA was extracted from 0.3 g of crushed rocks using NucleoSpin® Plant II Kit (Macherey-Nagel, Gmbh & Co. KG, Duren, Germany) according to the manufacturer’s instructions and quantified by Quant-iT dsDNA HS assay kit (Invitrogen molecular probes-Eugene ...
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bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
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bioRxiv - Biochemistry 2019Quote: Genomic DNA was isolated using the NucleoSpin® RNA II and NucleoSpin® RNA/DNA Buffer Set kit (MACHEREY-NAGEL, Düren, Mannheim, Germany) kit according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were extracted from fresh haploid mycelium after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel, Düren, Germany). Sequencing was performed using the Illumina HiSeq 2500 paired-end technology (Illumina Inc. ...
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bioRxiv - Genomics 2019Quote: ... placed in 90% ethanol and then stored dried until gDNA extraction was performed using the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany). Genomic DNAs from individual fish were then quantified using a Qubit fluorometer (Thermofisher ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated from the peripheral blood and tail tissues of each chimeric mouse 30 days after BM transfer using a NucleoSpin tissue kit (Macherey-Nagel, Duren, Germany). Then ...
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bioRxiv - Neuroscience 2020Quote: ... The total proteins were precipitated and prepared for Polyacrylamide Gel Electrophoresis using protein precipitator and resuspension buffer PSB/TCEP (Protein solving buffer and (tris(2-carboxyethyl)phosphine) TCEP reducing agent) from the NucleoSpin Triprep Kit (Macherey-Nagel, Hoerdt, France). The elution fraction containing RNA was further used for PCR analysis (see below) ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from leaves harvested at the indicated time point using the NucleoSpin® RNA Plus kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Plant Biology 2021Quote: Extractions of total genomic DNA were generated from silica gel-dried leaf material using the NucleoSpin Plant II kit (Macherey-Nagel, Dueren, Germany) or from herbarium specimens using the CTAB DNA isolation method as modified by Borsch et al ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted plant DNA of at least one diseased plant individual per site using the NucleoSpin® 96 Plant II kit (Macherey-Nagel, Germany). We obtained DNA for 134 out of the 171 diseased plant individuals ...
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Each worm pellet was re-suspended in 1M DTT and RA1 (solution from the NucleoSpin® RNA kit (Macherey-Nagel, Duren Germany #740955.50)) and frozen at −80°C overnight ...
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bioRxiv - Plant Biology 2022Quote: Leaves from 6- to 8-week-old Vicia faba plants were harvested and mesophyll RNA was extracted using the NucleoSpin Plant RNA extraction kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The genomic DNA of Rhodococcus strain IGTS8 was isolated using the NucleoSpin Tissue DNA Extraction kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 35 mg of animals were transferred into a 1.5 mL tube and total RNA was extracted using a Nucleospin® RNA Plus Kit (Macherey-Nagel, Düren, Germany). The prey cDNA library was screened for interactions with the 70 distal C-terminal amino acids of the TadNaC2 and TadNaC10 channels expressed in yeast from the bait vector pGBK-T7 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the samples were frozen and stored at −80 °C until the total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). The transcriptome was analyzed for three replicates from independent runs of the exposure experiment ...
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bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and products of the expected size were cut out and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). 10 ng of purified PCR product was then used as template for RNA probe synthesis ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA from 30 males and 30 females from the fish production unit of the fishing federation of Ille-et-Vilaine (Pisciculture du Boulet, Feins, France) was extracted with a NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The strain was grown overnight in de Man-Rogosa-Sharpe (MRS) medium (Carl Roth, Karlsruhe, Germany) and DNA was extracted using the NucleoSpin 96 tissue kit (Macherey-Nagel, Düren, Germany), with an extra cell lysis step using 20 mg/mL of lysozyme (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2019Quote: ... Starting from overnight grown liquid cultures of respective pAGM9121 constructs (prepared as described in section 5) plasmid mixtures were prepared using a NucleoSpin Plasmid Kit (Macherey-Nagel, Düren, DE). Following the preparation ...
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bioRxiv - Plant Biology 2020Quote: Leaf material of soil grown plants was collected and homogenized in liquid nitrogen prior to extraction of RNA with the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s advice ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR products were analysed using 1.5% agarose gel electrophoresis in 1x Tris-borate-EDTA (TBE) buffer and purified from the gel using a NucleoSpin® gel and PCR clean-up kit (Macherey-Nagel, Germany).
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bioRxiv - Zoology 2019Quote: ... Total genomic DNA extraction was performed using the NucleoSpin™ Plant II DNA extraction kit (Macherey-Nagel GmbH and Co., Düren NRW, Germany).
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bioRxiv - Microbiology 2019Quote: ... and analyzed and purified using an agarose gel with subsequent gel extraction with the “PCR clean-up and Gel extraction” Kit from Macherey-Nagel (Düren, Germany). A total of 90 ng of DNA per lane was incubated with different molar excesses of purified MalR protein (3-fold and 10-fold molar excess ...
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bioRxiv - Immunology 2020Quote: ... The reactions were then run at 100V in a 1% agarose gel and the digest vectors were excised and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, USA) according to instructions set by the manufacturer ...
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bioRxiv - Genomics 2021Quote: ... Gel extraction is performed to isolate the PCR product with the correct size range using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). PacBio sequencing library preparation for both libraries was done by the Genomics Core Leuven (KU Leuven) ...
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bioRxiv - Physiology 2021Quote: ... mRNA specimens were isolated from the cells grown on collagen-gel in 24-well dishes using a NucleoSpin RNAplus RNA isolation kit (Macherey-Nagel, Dueren, Germany) and converted to cDNA with SuperScript IV VILO Master Mix (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: Leaf material of soil grown plants was collected and homogenized in liquid nitrogen prior to extraction of RNA with the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s advice ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... Correct clones were determined by sequencing and cultured for subsequent plasmid preparation using NucleoBond Xtra Midi/Maxi kits (Macherey-Nagel GmbH & Co. KG). For scaled CFPS reactions ...
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bioRxiv - Genomics 2022Quote: DNA extraction was performed from a single female 4th instar larva (ToLID ilPecGoss1) using the NucleoMag Tissue kit (Macherey-Nagel, Allentown, PA, USA). The high molecular weight DNA was prepared for PacBio sequencing using the SMRTbell Express Template Prep Kit 2.0 for Continuous Long Read (CLR ...
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bioRxiv - Microbiology 2022Quote: ... The total genomic DNA of the insect individual and its symbionts was extracted using the NucleoSpin Tissue XS Kit (MACHEREY-NAGEL, Duren, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cat#M0491S/L) and gel extractions were done with the Macherey-Nagel NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Cat#740609). DNA assemblies were carried out by In-Fusion Cloning (Takara Bio ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 ml of mutant culture from propagation round 3 were used for gDNA isolation using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). Whole genome sequencing was performed via the INVIEW Resequencing service for bacterial genomes up to 10 Mb with an Illumina standard genomic library provided by Eurofins Genomics (Ebersberg ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 x 109 cells were used for extraction of genomic DNA using the Macherey-Nagel NucleoSpin Microbial DNA purification kit (Macherey-Nagel, Düren, Germany). PCR-free libraries (microbial short insert libraries ...
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bioRxiv - Plant Biology 2022Quote: ... the rhizosphere and root subpools were purified on an agarose gel (amplicon ∼450 bp) using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany), whereas all other subpools were purified with CleanNGS beads ...
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bioRxiv - Genomics 2022Quote: ... The DNA band at 5 to 10kb size was collected for extraction with a NucleoSpin Gel and PCR Clean-up purification kit following the manufacturer’s instructions (Macherey-Nagel, cat# 740609.250, Germany), and was eluted with 15 μl NF water.
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Microbiology 2023Quote: Whole peripheral blood was collected from newly hatched chicks and total DNA was extracted using the NucleoSpin 96 Blood core kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).