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Citations for Macherey-Nagel GmbH :
2001 - 2050 of 2127 citations for Rabbit S100 Calcium Binding Protein A6 S100A6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from INS-1 832/13 cells 48h after transfection using the NucleoSpin extraction kit (Macherey-Nagel, Düren, Germany). The amount of isolated RNA was measured using the NanoDrop system ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from 8–12 mg of dried tissue using the NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting constructs were used for protoplast transformation through midiprep using the NucleoBond Xtra plasmid kit (Macherey-Nagel GmbH & Co.KG, Duren, German) as per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... Both gel purifications and PCR clean-ups were performed using the NucleoSpin® PCR and Gel Clean Up Kit (Macherey-Nagel, Germany). DNA concentrations and purities were measured on a NanoDropTM 2000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The total RNA of the brain samples was extracted according to the manufacturer’s protocol by applying the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). The quality and quantity of RNA were assessed at 260 nm using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... 700ng of the plasmid library was isolated from the liquid culture using the NucleoBond® PC 500 kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
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bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The remaining PCR reaction of the sample with gRNA–TAR and –p24 combined was run on a 1% agarose gel containing ethidium bromide and the two expected bands were cut out separately and purified using the Nucleospin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s manual ...
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bioRxiv - Molecular Biology 2023Quote: ... were then extracted from the agarose gel and the DNA purified following the manufacturer instruction (NucleoSpin® Gel and PCR Clean-up kit (MACHEREY-NAGEL)) ...
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bioRxiv - Genomics 2023Quote: Perca fluviatilis gDNA samples from 35 males and 35 females were extracted with the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... these cultures were incubated overnight (800 RPM, 37°C) and miniprepped the following day using a 96 Plasmid NucleoSpin kit (Macherey-Nagel #740625.1). The resulting plasmids were able to be sequenced verified for proper VH and VL insertion ...
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bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
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bioRxiv - Biochemistry 2023Quote: ... consecutive copies of 197 bp with modified 601 sequence were transformed into DH5α Escherichia coli strain and purified from four liters of culture by using plasmid DNA purification kit Nucleobond® PC 10000 (Macherey-Nagel). Three mg of the plasmids were digested with EcoRI HF (0.25 U/µg DNA ...
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bioRxiv - Evolutionary Biology 2023Quote: Bulk DNA of the meristematic region and the basal portions of the leaves was extracted using NucleoSpin Plant II kit (Macherey-Nagel, Germany). DNA concentration was determined using a Qubit Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in water ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL 1X TE buffer ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... and we collected bands containing arrays ≥2-plex at 550-650 bps and 650-800 bps to be purified separately using a NucleoSpin Gel Purification Kit (Macherey-Nagel 740609.250). We also collected the cut vector backbone ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL water ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolation of gDNA was conducted either by using a column-based method following manufacturer’s instructions (NucleoSpin Microbial DNA kit, Macherey-Nagel, Düren, Germany) or an automated system (King Fisher Duo Prime System ...
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bioRxiv - Microbiology 2023Quote: ... The dephosphorylated pEX18Tc vector and PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). TEDA reaction was then carried out by mixing 150 ng of pEX18Tc with the corresponding PCR products at a molar ratio of 1:4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The NaOH was neutralized by the addition of 1 M HCl and the cDNA was purified by a PCR-clean-up using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... to pellet the cell debris and the RNA was extracted from the supernatant using the two column-system of the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Germany). DNA removal was ensured by the on-column DNA digestion according to the manufacturer’s description ...
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bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
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bioRxiv - Microbiology 2019Quote: Environmental DNA was extracted from 0.3 g of crushed rocks using NucleoSpin® Plant II Kit (Macherey-Nagel, Gmbh & Co. KG, Duren, Germany) according to the manufacturer’s instructions and quantified by Quant-iT dsDNA HS assay kit (Invitrogen molecular probes-Eugene ...
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bioRxiv - Cancer Biology 2020Quote: ... GRANTA-519 and B-cells purified from four MCL patients using the NucleoSpin® RNA II kit according to the manufacturer’s protocol (Macherey-Nagel, Oensingen, Switzerland). A minimum of 2µg per samples were used for analysis ...
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bioRxiv - Biochemistry 2019Quote: Genomic DNA was isolated using the NucleoSpin® RNA II and NucleoSpin® RNA/DNA Buffer Set kit (MACHEREY-NAGEL, Düren, Mannheim, Germany) kit according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: ... were extracted from fresh haploid mycelium after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel, Düren, Germany). Sequencing was performed using the Illumina HiSeq 2500 paired-end technology (Illumina Inc. ...
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bioRxiv - Genomics 2019Quote: ... placed in 90% ethanol and then stored dried until gDNA extraction was performed using the NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany). Genomic DNAs from individual fish were then quantified using a Qubit fluorometer (Thermofisher ...
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bioRxiv - Cancer Biology 2019Quote: ... genomic DNA was isolated from the peripheral blood and tail tissues of each chimeric mouse 30 days after BM transfer using a NucleoSpin tissue kit (Macherey-Nagel, Duren, Germany). Then ...
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bioRxiv - Plant Biology 2020Quote: ... Total RNA was isolated from leaves harvested at the indicated time point using the NucleoSpin® RNA Plus kit (Macherey-Nagel, Düren, Germany). cDNA was synthesized from 2 μg of total RNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific ...
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bioRxiv - Plant Biology 2021Quote: Extractions of total genomic DNA were generated from silica gel-dried leaf material using the NucleoSpin Plant II kit (Macherey-Nagel, Dueren, Germany) or from herbarium specimens using the CTAB DNA isolation method as modified by Borsch et al ...
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bioRxiv - Evolutionary Biology 2020Quote: We extracted plant DNA of at least one diseased plant individual per site using the NucleoSpin® 96 Plant II kit (Macherey-Nagel, Germany). We obtained DNA for 134 out of the 171 diseased plant individuals ...
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bioRxiv - Bioengineering 2019Quote: ... genomic fragments containing the gRNA target site were amplified with PCR and purified using NucleoSpin Gel and a PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Then 200 ng of each of the PCR products derived from the transfected and non-transfected cells were denatured at 95° C for 5 min and reannealed ...
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bioRxiv - Plant Biology 2021Quote: ... The amplicons were purified from the PCR reagent tube via the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Each worm pellet was re-suspended in 1M DTT and RA1 (solution from the NucleoSpin® RNA kit (Macherey-Nagel, Duren Germany #740955.50)) and frozen at −80°C overnight ...
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bioRxiv - Plant Biology 2022Quote: Leaves from 6- to 8-week-old Vicia faba plants were harvested and mesophyll RNA was extracted using the NucleoSpin Plant RNA extraction kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The genomic DNA of Rhodococcus strain IGTS8 was isolated using the NucleoSpin Tissue DNA Extraction kit (Macherey-Nagel, Lab Supplies Scientific SA, Hellas) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... was linearized by AdeI restriction enzyme digestion and DNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). For in-vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 35 mg of animals were transferred into a 1.5 mL tube and total RNA was extracted using a Nucleospin® RNA Plus Kit (Macherey-Nagel, Düren, Germany). The prey cDNA library was screened for interactions with the 70 distal C-terminal amino acids of the TadNaC2 and TadNaC10 channels expressed in yeast from the bait vector pGBK-T7 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... the samples were frozen and stored at −80 °C until the total RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany). The transcriptome was analyzed for three replicates from independent runs of the exposure experiment ...
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bioRxiv - Epidemiology 2020Quote: ... 20 µL of Proteinase K were added to 180 µL of crushed tick sample and DNA was extracted using the NucleoSpin® 96 virus Core kit (Macherey-Nagel, Germany) and the automatic platform Biomek4000 (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and products of the expected size were cut out and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Duren, Germany). 10 ng of purified PCR product was then used as template for RNA probe synthesis ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA from 30 males and 30 females from the fish production unit of the fishing federation of Ille-et-Vilaine (Pisciculture du Boulet, Feins, France) was extracted with a NucleoSpin Kit for Tissue (Macherey-Nagel, Duren, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The strain was grown overnight in de Man-Rogosa-Sharpe (MRS) medium (Carl Roth, Karlsruhe, Germany) and DNA was extracted using the NucleoSpin 96 tissue kit (Macherey-Nagel, Düren, Germany), with an extra cell lysis step using 20 mg/mL of lysozyme (Sigma-Aldrich ...