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Citations for Macherey-Nagel GmbH :
1951 - 2000 of 2127 citations for Rabbit S100 Calcium Binding Protein A6 S100A6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Clonal virus populations were characterized by isolating viral RNA from 150 µl aliquots of the cell culture supernatant with the NucleoSpin® RNA Virus kit (MACHEREY-NAGEL) followed by conventional RT-PCR and bidirectional Sanger sequence analysis.
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bioRxiv - Zoology 2021Quote: ... standard DNA extraction was performed using the NucleoSpin™ Plant II DNA extraction kit (Macherey-Nagel GmbH and Co., Düren NRW, Germany). A reasonable number (usually 30–70 ...
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bioRxiv - Microbiology 2022Quote: ... Viral genome equivalents (vge) were determined by qPCR of DNA isolated from encapsidated virions isolated with NucleoSpin Blood kit QuickPure (Macherey-Nagel; 740569.250).
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bioRxiv - Plant Biology 2021Quote: ... total RNA was extracted from root tissues of DKC-9144 and PG-2475 at three time points (15, 25 and 35 DAS) with or without PBZ using Nucleospin RNA plant kit (Pal et al., 2017) (Macherey-Nagel, Germany). cDNA synthesis and qPCR was performed as described previously (Pal et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... and the band with the expected size excised and purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The second PCR products were sent for Sanger sequencing to Eurofins Genomics and checked for quality ...
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bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) products and plasmid digestion derivatives were purified using NucleoSpin® Gel and PCR Clean-up kits (Macherey-Nagel). Plasmids were extracted from E ...
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bioRxiv - Microbiology 2020Quote: ... lung left inferior lobe from hamsters were cut into pieces and lysed with RA1 lysis buffer provided with the NucleoSpin® RNA Plus kit (Macherey-nagel), RNA extraction was performed according the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2022Quote: gDNA from 6 male and 5 female pupae (sexed by eye) was extracted using the NucleoSpin Tissue kit (Macherey-Nagel, Düren, Germany), pooled by sex and used as template for subsequent PCRs ...
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bioRxiv - Genetics 2022Quote: RNA extraction was performed from frozen tissue in culture plates (P6 well plate, Falcon®) by using the NucleoSpin RNA kit (Macherey-Nagel). RNA concentration was measured with NanoDrop1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: Cells pellet (1.5*10^6) were collected at different time points after LPS stimulation and DNA was extracted with the Nucleospin tissue kit (Macherey-Nagel, 740952). The genomic DNA was eluted in 50 μl of BE buffer (5 mM Tris/HCl ...
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bioRxiv - Bioengineering 2020Quote: Standard methods were used to determine the TSS and volatile suspended solids concentration.25 The COD was measured using test kits (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... RNA extraction was performed using a modified protocol of the NucleoSpin 96 RNA tissue kit (Macherey-Nagel Gmbh&Co. KG, Düren, Germany) and using a vacuum system [12] ...
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bioRxiv - Genomics 2020Quote: ... Cleaved Histone-DNA complexes were isolated by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel, 740609).
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bioRxiv - Zoology 2020Quote: Approximately 50-100 mg of tissue (from the second pereopod) was used for DNA extraction using the NucleoSpin® Tissue Kit (Macherey-Nagel) following the instructions of the manufacturer ...
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bioRxiv - Genomics 2019Quote: ... while total RNA was extracted using the Nucleospin RNA plant kit with the protocol modified for maximum yield (Macherey-Nagel, Oensingen, Switzerland). RNA concentration was determined with a Qubit 3.0 (Thermofisher).
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bioRxiv - Plant Biology 2019Quote: Total RNA from 4 week old Arabidopsis WT Col-0 and mtamutants28 was extracted as described above followed by polyA enrichment using NucleoTrap® mRNA isolation kit (Macherey-Nagel). Immunoprecipitation was performed with EpiMark N6-Methyladenosine Enrichment Kit (NEB ...
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bioRxiv - Neuroscience 2021Quote: Viral biodistribution analysis - Genomic DNA (gDNA) was extracted from the brain region of interest with the NucleoSpin®Tissue Kit (Macherey-Nagel) extraction kit and 100 ng of gDNA was used as a qPCR template ...
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bioRxiv - Microbiology 2020Quote: We extracted DNA from the biomass pellets stored at −20°C using a NucleoSpin® Microbial DNA Kit (Macherey-Nagel, Düren, Deutschland), according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2020Quote: ... urticae (one butterfly individual per nest) collected across 8 sites spread across the latitudinal gradient using the NucleoSpin® 96 Tissue kit (Macherey-Nagel) (Figure 1) ...
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bioRxiv - Plant Biology 2019Quote: ... Extraction of total genomic DNA was conducted with the NucleoSpin Plant Kit in accordance to the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). The concentration of the DNA samples was checked with a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... All single fragment PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Overlap PCRs were carried out using 100 ng of each purified PCR products and the resulting fragment of interest was purified from agarose gel ...
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bioRxiv - Genetics 2019Quote: ... Total genomic DNA was extracted from a fresh stem slice using NucleoSpin® Plant II DNA extraction kit (Macherey-Nagel, Düren, Germany) and quantified using a Qubit 2.0 device (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... to reach at least 200x colonies per guide-RNA and the library plasmid pool was purified using NucleoBond Xtra-Maxi Kit (Macherey-Nagel, #740414.50). Then ...
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bioRxiv - Microbiology 2020Quote: Lung left inferior lobes from hamsters were cut into pieces and lysed using the RA1 lysis buffer provided with the NucleoSpin® RNA Plus kit (Macherey-Nagel). RNA extraction was then performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA extraction was performed on (i) the frozen suspension and (ii) the collected CFU suspension with the NucleoSpin® 96 Food kit (Macherey-Nagel) following the supplier’s recommendations.
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bioRxiv - Immunology 2021Quote: ... The amplified PCR product is then purified from gel using the “Nucleospin Gel and PCR Clean-up” kit (Macherey-Nagel, Reference: 740609.250).
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bioRxiv - Genomics 2021Quote: Sequencing libraries were prepared from the cells collected at the end of the screens as follows: Genomic DNA was extracted using the NucleoSpin® Blood XL kit (Macherey-Nagel) with a condition of 50e6-100e6 cells per column ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from 1 mL co-culture taken from different time points during the cultivation experiment using the NucleoSpin Tissue DNA extraction kit according to the manufacturer’s instructions (Macherey-Nagel, Düren, Germany). Cell numbers were quantified by direct epifluorescence microscopy of Sybr-green stained cells immobilized on agarose-coated slides to generate standard curves for qPCR as described previously [42] ...
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bioRxiv - Genomics 2019Quote: ... CHP-100 and HS-SYII) and tissue (ES1 and RH) was extracted by using the column-based NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... PCR reactions were realized using Phusion High-Fidelity DNA Polymerase (ThermoScientific) and PCR products were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). The overlap PCR product was purified from agarose gel and was directly used to transform wild type F ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from 2 × 105 cells untreated or butyrate-conditioned BMDMs using the NucleoSpin RNA extraction kit (Macherey-Nagel, 740902.50) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Cell Biology 2022Quote: ... MuSCs from the gaskin muscles were collected by FACS and then subjected to extract RNA with NucleoSpin® RNA XS kit from Macherey-Nagel. 1 ng high-quality RNA per sample were reverse transcribed and amplified into cDNA following Smart-seq2 library preparation protocol (Picelli et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... plasmids were linearized by AscI (pBSK vector) or SfiI (pYX vector) and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609). cRNA was produced by in vitro transcription using mMessage mMachine™ T3 kit (Ambion ...
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bioRxiv - Immunology 2022Quote: ... 5000-10 000 ThyMCs were sorted into lysis buffer and the RNA was extracted using the NucleoSpin® RNA XS RNA isolation kit (Macherey-Nagel). Total RNA was reverse transcribed into cDNA using the SuperScript IV First-Strand Synthesis System (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from mock- and KSHV-infected blood or lymphatic ECFCs using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). RNA was further concentrated and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Neuroscience 2022Quote: Cells were lysed (RLT buffer with 1% β-mercaptoethanol) and RNA was isolated via column purification (NucleoSpin RNA kit, Macherey-Nagel 740955). RNA integrity and concentration were determined with BioAnalyzer (Agilent ...
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bioRxiv - Plant Biology 2022Quote: ... O23 and d44a genomic DNA was extracted from liquid-cultured aerial hyphae using the NucleoBond high-molecular-weight DNA kit (MACHEREY-NAGEL, Germany). The genomic DNA was processed through the short-read eliminator kit XL (Circulomics) ...
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A conserved isoleucine in the binding pocket of RIG-I controls immune tolerance to mitochondrial RNAbioRxiv - Immunology 2022Quote: ... RNA bands according to the size of the 18s and 28s rRNA were cut from the agarose gel and extraction was performed using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel). The extraction was performed according to the manufactureŕs protocol for RNA extractions from agarose gels using the buffer NTC (Macherey Nagel).
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bioRxiv - Biophysics 2022Quote: ... Plasmid purifications for mammalian cell lines transfection were performed using high-yield column-based commercial NucleoBond Xtra Midi kit (Macherey-Nagel, Germany) following usage protocols.
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bioRxiv - Evolutionary Biology 2023Quote: ... The resulting powder was used for DNA extraction using the NucleoSpin® Tissue kit following manufacturer instructions (MACHEREY-NAGEL GmbH & Co. KG). DNA quantity and purity were checked with a Nanodrop One spectrophotometer (Thermo Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... The two resulting PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Ref. REF 740609.50; Macherey-Nagel, Düren, Germany), digested at 37°C overnight using FastDigest Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The PCR products from the same sample were pooled and purified over 2% agarose gels (band size at 275 bp) using NucleoSpin® Gel and PCR clean up kit (Macherey-Nagel). Purified amplicons were quantified by Qubit (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... Bacteria were grown in Luria-Bertani Broth at 37°C and plasmid DNA was isolated using the Nucleobond Xtra midi kit (MACHEREY-NAGEL, 740410), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was extracted from microglia after CD11b microbead isolation from P7 neonates born to and nursed by WT and Slc2a5−/− dams using the NucleoSpin RNA kit (Macherey-Nagel, 740955.250). The QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Physiology 2023Quote: ... six samples each containing 5 whole larvae or 5 larval dissected tissues (central neuronal systems or body carcasses) were disrupted in 350 μl lysis buffer from the NucleoSpin RNA kit (Macherey-Nagel, #740955) containing 1% beta-mercaptoethanol ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from a cell pellet obtained from 2-ml aliquots of the microbial cultures using the tissue DNA extraction kit (Macherey-Nagel, Germany). The amoA gene of Ca ...
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bioRxiv - Immunology 2023Quote: Total RNA was prepared from XG6 cells treated or not with IgG-ASO or CTR (48h, 3µM) using the NucleoSpin® RNA L kit (Macherey-Nagel, France) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1.2 l LB-medium and incubated for 4 h at 37 °C before extracting plasmid DNA using Nucleobond Xtra Midi kit (Macherey-Nagel, C640003) according to the manufacturer’s protocol.
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bioRxiv - Genetics 2023Quote: Genomic DNA of the 54 genotypes was extracted from the leaves using a mixed alkyltrimethylammonium bromide buffer and the kit NucleoMag Plant (Macherey-Nagel, Germany). DNA samples were quantified using ThermoFisher Scientific fluorometry Qubit ...