Labshake search
Citations for Macherey-Nagel GmbH :
1951 - 2000 of 2265 citations for Human Probable fructose 2 6 bisphosphatase TIGAR TIGAR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... primers 4163-4183 and 4288-4308) were amplified by PCR (Rotor Gene 3000, Corbett Research) from 50ng DNA (Nucleospin Tissue kit, Macherey-Nagel). PCR products quantified with Sybr green (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... the total genomic DNA from plasmids was extracted from fresh transformed bacteria using a plasmid DNA Maxi Prep kit (Nucleobond XtraMaxi EF, Macherey-Nagel). Transgenic control GFP fluorescent marker expressing line (NB30 ...
-
bioRxiv - Plant Biology 2020Quote: ... by electrophoresis at 90 V for 1 h and the 1.5 kb target band was collected for purification using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instruction prior to sequencing ...
-
bioRxiv - Evolutionary Biology 2021Quote: We extracted both DNA and RNA from the RNAlater samples using the Nucleospin XS Kit in combination with the Nucleospin RNA/DNA Buffer Set (Macherey-Nagel), and we extracted DNA from the ethanol samples using the DNeasy Blood and Tissue kit (Qiagen ...
-
bioRxiv - Genomics 2021Quote: ... Resulting amplicons were loaded on a 1% agarose gel and purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Libraries were sequenced paired-end 75bp on the NextSeq 500 platform yielding approximately 4*106 fragments per sample (Supplementary Table 2) ...
-
bioRxiv - Genomics 2021Quote: ... by loading 400 ng of each pooled sample on an agarose gel followed by a cleaning step using the Nucleospin Gel and PCR Cleanup kit (Macherey-Nagel). Samples were PCR amplified using the Phusion High-Fidelity DNA Polymerase (New England Biolabs ...
-
bioRxiv - Genomics 2019Quote: ... DNA was extracted from fish fin clips for the ddRADseq and muscle tissue for the epiRADseq libraries using the NucleoSpin Tissue kit (Macherey-Nagel) following the manufacturers recommendations ...
-
bioRxiv - Microbiology 2019Quote: RNAs were isolated from ceca and ileum tissue biopsies using NucleoSpin RNA purification kit (Macherey-Nagel, GmbH & co. KG, Düren, Germany) according to the manufacturer’s protocol with the following modifications ...
-
bioRxiv - Genetics 2021Quote: ... 500ml of LB media with Ampicillin were inoculated with the rest of the mixture and incubated overnight for subsequent plasmid purification using the NucleoBond Xtra Maxi Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... and a ∼500 bp portion around the predicted CRISPR cut site was amplified by PCR and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). This fragment was then ligated into an EGFP-N vector using Xho1 and Apa1 restriction sites and transformed into DH5-a competent cells ...
-
bioRxiv - Cell Biology 2021Quote: ... flanking Cas9 targeted site was amplified from genomic DNA extracted from wild type MCF10A cells (NucleoSpin tissue extraction kit, Macherey-Nagel), Puro-T2A were amplified from the custom-made plasmid MXS Puro bGHpA using primers containing T2A sequence ...
-
bioRxiv - Neuroscience 2021Quote: ... The resulting SORL1-202 amplicons were spin-column purified using a Nucleospin Gel and PCR Clean-up Kit (#740609, Macherey-Nagel) prior to validation by DNA sequencing ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was extracted from 30 mg of lice (15 lice) using the Total RNA isolation Nucleospin RNA kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2020Quote: ... Synchronized ring stage parasites were transfected with 100 – 200 μg plasmids purified by NucleoBond Xtra Midi EF midiprep kit (Macherey-Nagel) by electroporation using Bio-Rad laboratories gene pulser X cell31 ...
-
bioRxiv - Cell Biology 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
-
bioRxiv - Microbiology 2021Quote: ... selected clones were grown overnight at 37°C under agitation and the plasmid DNA was extracted and purified using the NucleoSpin plasmid Mini or Midi kit (Macherey-Nagel).
-
bioRxiv - Immunology 2021Quote: ... RNA was extracted from flow-sorted naive CD8+ T cells (n = 300 per condition) using a NucleoSpin RNA XS Kit (Macherey-Nagel), and cDNA was synthesized using Reverse Transcription Master Mix (Fluidigm) ...
-
bioRxiv - Immunology 2021Quote: ... identical conditions were used except DMEM was used instead of HCM media and RNA extractions were performed using only the NucleoSpin RNA kit (Macherey-Nagel). Where ruxolitinib treatment is indicated ...
-
bioRxiv - Cell Biology 2021Quote: Six replicate RNA preparations for each genotype or condition were prepared using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, #740984.50) kit ...
-
bioRxiv - Genomics 2021Quote: ... genomic DNA was extracted from bacterial strains grown on King’s medium B (King et al. 1954) at 28°C for 24 h using NucleoSpin Microbial DNA kit (Macherey-Nagel, Germany). The quality of the genomic DNA was assessed by electrophoresis in 0.8% agarose gel.
-
bioRxiv - Genomics 2019Quote: ... One colony per construct was grown overnight in a shake incubator at 37°C before plasmid extraction using the NucleoSpin Plasmid Transfection-grade kit (Macherey-Nagel). For each construct three biological replicates were performed by transfecting the plasmids into 80% confluent cells of MM001 in a 24 well plate ...
-
bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from mESCs and MEFs (3 biological replicates of WT and Atxn7l3−/− for each cell types) using the NucleoSpin RNA isolation kit (Macherey-Nagel), according to manufacturer’s instructions ...
-
bioRxiv - Genetics 2020Quote: ... DNA was eluted from the beads in Tris-EDTA buffer and cleaned up using the Nucleospin Gel and PCR Purification kit (Macherey-Nagel). For every cell line ...
-
bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from U251 cells (SETD6 control vs. KO or Empty vs. TWIST1 WT) using the NucleoSpin RNA Kit (Macherey-Nagel). Samples were prepared in triplicates (SETD6 KO ...
-
bioRxiv - Immunology 2022Quote: ... The surviving cells were expanded for 24 h and DNA was extracted using a NucleoSpin Tissue kit (Macherey-Nagel, Cat#740952). An additional eight biological replicates were similarly prepared but did not undergo killing by TC5A87 ...
-
bioRxiv - Biochemistry 2022Quote: ... pre-tRNAPheGAA2-2 and human pre-tRNATyrgta8-1 and tRNAArgtct3-2 were amplified by PCR of respective pUC19 vectors and template DNA was isolated using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). RNA substrates were produced by run-off in vitro transcription using T7 RNA polymerase (New England Biolabs ...
-
bioRxiv - Biochemistry 2022Quote: Total RNAs were extracted from mid-log yeast cultures grown 16 h at 20°C in YPD as described above using the Nucleospin RNA II kit (Macherey-Nagel) and were reverse transcribed with Superscript-II reverse transcriptase (Invitrogen) ...
-
bioRxiv - Immunology 2022Quote: ... Transfection-grade plasmid DNA was isolated from XL10Gold cell overnight culture with the NucleoBond™ Xtra Midi Kit (Macherey-Nagel, Germany).
-
bioRxiv - Genetics 2022Quote: ... 500 ml of LB media with Ampicillin were inoculated with the rest of the mixture and incubated overnight for subsequent plasmid purification using the NucleoBond Xtra Maxi Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The amplicon with the expected size was excised from the agarose gel and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). In order to induce a DNA double-strand break in close proximity to the site of insertion ...
-
bioRxiv - Molecular Biology 2022Quote: ... and purified from overnight cultures in LB medium containing 100 μg/ml Ampicillin grown at 37°C by using the NucleoSpin Plasmid isolation kit (Macherey-Nagel). The selection markers for fungal transformation were cloned into digested plasmids by using the Rapid DNA Ligation Kit (Thermo) ...
-
bioRxiv - Microbiology 2022Quote: Total RNA was isolated from three independent experiments of the mock- and KSHV-infected lymphatic ECFCs isolated as an adherent subpopulation from donor 1 using the NucleoSpin RNA extraction kit (Macherey-Nagel), after which purity and concentration was determined with NanoDrop spectrophotometer (Thermo Scientific) ...
-
bioRxiv - Plant Biology 2022Quote: ... Plasmids were extracted from positive colonies using 5 mL liquid cultures with the NucleoSpin Plasmid Purification kit (Macherey-Nagel; Ref.: 11932392) and sequenced (GATC ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from only the 13C-acetate grown cultures that yielded enough DNA at 94 days of incubation using the NucleoSpin Microbial DNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from the roots of 21-days old mock- and SA190-colonized Arabidopsis grown on ½ MS with or without 25% PEG with the Nucleospin RNA plant kit (Macherey-Nagel) following the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
-
bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
-
bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were extracted from agarose gel (1.5%, 1 h, 100 V) by using NucleoSpin® Gel and PCR Cleanup XS kit (Macherey-Nagel) and the amplicon size and DNA concentration were checked with High Sensitivity D1000 ScreenTape assay in 2200 TapeStation System (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2022Quote: Total RNA of five corrected hiPSC clones and the naïve parental iPSC line was isolated using the Macherey-Nagel NucleoSpin RNA mini kit (Macherey-Nagel, 740955) according to manufacturer’ s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... The 0.22 µm membrane was used for bacterial DNA isolation using NucleoMag DNA/RNA Water Kit (MACHEREY-NAGEL Inc., Bethlehem, PA) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... at least eight separate tubes were used for PCR and then pooled and purified by using PCR and gel extraction kit (Macherey-Nagel) according to manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... The BAG6 target region was amplified from the genomic DNA from all samples using Q5 High-Fidelity Master Mix and subsequently purified using a NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. The purified samples were sent for sanger sequencing and the proportion of BAG6 knockout cells in each sample was estimated using the ICE CRISPR analysis tool (https://www.synthego.com/products/bioinformatics/crispr-analysis).
-
bioRxiv - Cell Biology 2022Quote: Total RNA was isolated from control and IMCD PCM1 KO cells before SAG treatment and 24 h after SAG treatment using NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
-
bioRxiv - Physiology 2024Quote: ... standard incubation control and after 1,2,4 h at hypoxic conditions) was extracted using the NucleSpin RNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: DNA was extracted from blood samples of patients II-8 and IV-1 using the Nucleospin extraction kit (ref 740952-Macherey-Nagel). Subsequently ...
-
bioRxiv - Microbiology 2024Quote: DNA was extracted using 100μl of the homogenate of crushed ticks according to the Genomic DNA from tissue kit (Macherey-Nagel, Germany) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated from whole plant tissue ground in liquid nitrogen using the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: RNAs from whole lungs or BM of naive or IAV-infected mice were extracted and purified using the NucleoSpin RNA Extraction Kit (Macherey-Nagel) according to the manufacturer’s instructions ...