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Citations for Macherey-Nagel GmbH :
1751 - 1800 of 2265 citations for Human Probable fructose 2 6 bisphosphatase TIGAR TIGAR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from roots of plants 5 days after germination using RNA protein purification kit (Macherey-Nagel). Next generation sequencing (NGS ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
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bioRxiv - Cancer Biology 2022Quote: ... plasmid DNAs were recovered from ampicillin resistant colonies using a kit (NucleoBond Xtra Midi, Macherey-Nagel GmbH, Düren, Germany), and the nucleotide sequences of each construct confirmed as correct by Sanger direct cycle sequencing using a U6-specific sequencing primer.
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bioRxiv - Plant Biology 2022Quote: ... for 10 min at 37°C and then cleaned up with the NucleoSpin RNA clean-up kit (Macherey-Nagel). RNA quality was verified on a 2100 Bioanalyzer Instrument (Agilent ...
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bioRxiv - Molecular Biology 2022Quote: ... Certain PCR products were purified from the gel using NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from DIV4 or DIV6 primary neuronal cultures using the NucleoSpin RNA purification kit (Macherey-Nagel) and from human fibroblasts using Trizol reagent (Invitrogen) ...
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bioRxiv - Genetics 2024Quote: BAC DNA was extracted from BAC clones (BacPac Resources, CHORI, Oakland) using the Nucleobond Xtra BAC kit (Macherey-Nagel) and subsequently labeled (Nick translation protocol ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA for quantitative reverse transcription PCR (qRT-PCR) was isolated using the NucleoSpin® RNA Kit (Macherey-Nagel), including DNA digestion ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
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bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Agilent microarray analyses were performed from isolated total RNA using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250). Per reaction 200 ng RNA was amplified and Cy3-labeled using the LIRAK kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: RNA from nasal washings and organ samples was extracted using the NucleoMag® VET Kit (Macherey-Nagel, Düren, Germany) in combination with a Biosprint 96 platform (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was extracted from ethylenediaminetetraacetic acid (EDTA)-anticoagulated blood using NucleoSpin Blood Quick Pure kit (Macherey-Nagel, Duren, Germany) and was stored in the gene bank run by Azabu University (Kanagawa ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: RNA isolation for multiple tissues was achieved by using the NucleoZOL one phase RNA purification kit (Macherey-Nagel #740404.200) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: Total RNA extraction and complementary DNA (cDNA) synthesis were performed using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) and High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA was extracted from TGF-β1-treated cells (as described above) using NucleoSpin® RNA kit (Macherey-Nagel) according to manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: All expression constructs generated in this work were then midi-prepped using NucleoBond Xtra Midi kit (Macherey-Nagel 740410.50).
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bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
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bioRxiv - Genetics 2023Quote: ... except for genomic DNA for whole-genome sequencing that was extracted using NucleoSpin DNA purification kit (MACHEREY-NAGEL®).
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bioRxiv - Cancer Biology 2023Quote: RNA from sorted blasts or ex vivo-cultured cells was isolated using NucleoSpin RNA XS kit (#740902 Macherey-Nagel) or RNeasy Mini Kit (#74104 Qiagen ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then lysed in Buffer RA1 and RNA was extracted with NucleoSpin RNA kit (Macherey-Nagel ref 740902.250). Total RNAs were then retrotranscribed to cDNA using AffinityScript Multiple Temperature cDNA Synthesis Kit (Agilent ref 200436 ...
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bioRxiv - Immunology 2023Quote: ... The excised DNA fragments were further refined using nucleospin gel and by using PCR clean-up kit (Macherey-Nagel) and quantified for determining the purity and concentration ...
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bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
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bioRxiv - Physiology 2023Quote: DNA was extracted from 50µm cryostat sections of left ventricular tissue samples with NucleoMag Tissue Kit (Macherey-Nagel, Germany) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: DNA was extracted from 20 mg of dried leaf tissue using the NucleoSpin Plant II kit (Macherey-Nagel, Denmark). DNA was extracted according to the manufacturer’s protocol with the following modifications ...
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bioRxiv - Evolutionary Biology 2023Quote: We extracted genomic DNA from faeces using the NucleoSpin®Soil kit (Macherey-Nagel GmbH & Co. KG, Düren, Germany) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Cancer Biology 2023Quote: Total DNA was extracted from various mouse organs using the NucleoSpin Tissue kit (Macherey-Nagel Inc., Allentown, PA, USA). The DNAs were used for species-specific PCR with primers against human α-satellite and mouse plakoglobin genomic regions [35] ...
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bioRxiv - Genomics 2023Quote: A second purification step was performed using a PCR product extraction from gel agarose kit from Macherey-Nagel (MN) NucleoSpin® Gel and PCR Clean-up kit and restarting the above protocol from the step with the addition of CQW buffer then PW2 buffer.
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... DNA isolations were conducted using the NucleoSpin Plant II Mini Kit for DNA from plants (Macherey-Nagel, Düren, Germany). Preliminary quality assessments were conducted using agarose gel visualization and NanoDrop (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... total DNA was extracted from ∼300 mg of soil using the NucleoSpin Soil 96 kit (Macherey-Nagel, Düren, Germany). Lysis was performed using the Buffer SL with Enhancer SX and on the FastPrep 24 homogenizer (MP Biomedicals ...
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bioRxiv - Biochemistry 2024Quote: RNA from 10 mL yeast culture was isolated using a mini kit for RNA isolation (Macherey-NAGEL, REF 740933.50). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting digested fragment was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, 740609.250). Guide RNAs were designed using the CRISPOR tool (http://crispor.gi.ucsc.edu/ ...
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bioRxiv - Microbiology 2024Quote: Total RNA from lung tissue cells for hamsters was isolated using NucleoSpin RNA Kit (740955.250, MACHEREY-NAGEL, Duren, Germany). cDNA libraries were prepared by KAPA mRNA HyperPrep Kit following the standard protocol ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was extracted from the pellet using the instructions provided by a commercial kit (Macherey-Nagel GmbH & Co.). Each reaction consisted of 500 nM of primer FWD and REV (Table S3 ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from 100 mg of ripe fruits using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed at the Ecole normale superieure genomic core facility (Paris ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated as indicated for 72 hours prior to harvesting of RNA using the Nucleospin RNA kit (Macherey-Nagel). First-strand cDNA synthesis was performed using iScript Supermix (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: RNA was purified from cerebellum samples from Cockayne patients and age- and gender-matched healthy individuals by using the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2019Quote: ... lysates prepared from infected and stimulated cells were collected and RNA extracted using a NucleoSpin RNA kit (Macherey-Nagel, 740955). The yield and purity of the extracted RNA was assessed using a spectrophotometer (DS-11 FX ...