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Citations for Macherey-Nagel GmbH :
151 - 196 of 196 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... undecanal-tagged peptides and excess reagents were removed by passing the reaction through HRX-reverse phase spin-columns (Macherey-Nagel GmbH & Co, KG, Dueren, Germany). The flow-through containing the enriched N-termini were dried with a SpeedVac to a remaining volume of ~50 μL ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from 12-day-old plantlets grown in vitro using the RNA Plus Kit (Macherey-Nagel GmbH & Co. KG, Düren, NRW, Germany) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... for the ON-state selection was prepared by dissolving appropriate amounts of kanamycin in deionised water and filter-sterilising it through a 0.2 µm cellulose acetate filter (MN Steriliser CA, Macherey-Nagel GmbH & Co. KG, Düren, Germany). The L-arabinose (Tokyo Chemical Industry Co. ...
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bioRxiv - Bioengineering 2024Quote: ... and PA 6,6 dimer) were analyzed using a Shimadzu prominence LC system together with a C18-RP column (NUCLEOSIL 100-5 C18, Macherey-Nagel GmbH & Co. KG, Germany). Analytes were quantified via a photodiode array (Nexera X2 SPD-M30A ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in PBS with 20 µg/ml PI and 0.2 mg/mL RNAse A (Macherey-Nagel) and incubated at RT for 30 min before analysis on a FACSCalibur (Beckton Dickinson) ...
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bioRxiv - Plant Biology 2024Quote: The tRNA fraction was isolated from 17 µg of total RNA using Nucleobond AX-20 columns (Macherey-Nagel) following a rescaled protocol based on (28) ...
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bioRxiv - Plant Biology 2020Quote: ... DNA was extracted using either a MO BIO PowerPlant DNA Isolation Kit (MO BIO Laboratories, Carlsbad, California, USA) or NUCLEOSPIN Plant II Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany). Dry plant leaf material (typically ...
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bioRxiv - Microbiology 2023Quote: ... DisA was incubated with unlabeled ATP and 15 µl of the reaction product was analysed by thin layer chromatography (TLC; POLYGRAM CEL300 PEI plates) (Macherey-Nagel GmbH & Co. KG, Düren, Germany) using a running buffer of [1 vol ...
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bioRxiv - Biochemistry 2024Quote: Products formed in in vitro reactions were quantified via GC-FID using a non-chiral OPTIMA™ 5MS column (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Substrates and products were directly extracted from the aqueous reaction solutions with DCM containing 2 mM acetophenone as an internal standard ...
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bioRxiv - Cell Biology 2020Quote: ... were purified using an endonuclease free plasmid extraction kit (NucleoBond Xtra Maxi Plus EF, Macherey-Nagel, cat# 740426.10).
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bioRxiv - Neuroscience 2021Quote: ... Endotoxin-free plasmid DNA was purified from clones with correct sequences (Nucleobond Xtra Maxi EF, Macherey-Nagel Inc.), and re-suspended in endotoxin-free water.
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bioRxiv - Cancer Biology 2024Quote: ... plasmid DNA was extracted using the NucleoBond Xtra Maxi kit for endotoxin-free plasmid DNA (Macherey-Nagel, 740424.50) and eluted in 400 µL of nuclease-free water ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Biochemistry 2020Quote: ... After proteolytic cleavage the protein was incubated with 1.5 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) for 25 min at 4°C to remove His6-SUMO (for purification of SUMO-Hsc70 overnight digestion with Ulp1 and second incubation with Protino Ni-IDA was omitted) ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 g of soil were used for DNA extraction using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: Products formed in in vitro or whole-cell reactions were quantified via GC-FID using a non-chiral OPTIMA™ 5MS column (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Substrates and products were directly extracted from the aqueous reaction solutions with DCM containing 2 mM acetophenone as an internal standard ...
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bioRxiv - Genomics 2024Quote: ... the DDM-free peptide samples were desalted with 0.5 % formic acid and eluted with 0.5 % formic acid/80 % acetonitrile using SepPak C18 cartridges (Waters, Milford, MA, USA) with the help of CHROMABOND® SPE vacuum manifold (Macherey-Nagel GmbH & Co. KG, Duren, Germany). Samples were then dried in a Savant SpeedVac SPD120 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Amplification of respective gene fragments was verified by Sanger sequencing of the amplicons after separation by agarose gel electrophoresis and purification (NucleoSpin® Gel and PCR Clean-up kit, Macherey-Nagel GmbH & Co. KG, Düren, Germany). Individual plants were propagated to obtain homozygous mutants and wildtypes and were phenotyped for EPH ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were purified from cell-free lysate by metal ion affinity chromatography with Protino Ni-NTA Agarose (Macherey-Nagel) as matrix followed by ultrafiltration (Amicon Ultra-15 centrifugal filter units ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from the youngest leaf tissues of three biological replicates of DM-susceptible and DM-resistant yellow maize inbred lines (mock-inoculated and DM-inoculated) using the modified NucleoSpin™ Mini Kit for RNA purification (Macherey-Nagel™, GmbH & Co. Du ren, Germany). One hundred fifty (150 ...
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bioRxiv - Plant Biology 2024Quote: DNA-free RNA was isolated with NucleoSpin® RNA Plant and Fungi Kit as recommended by the manufacturer (MACHEREY-NAGEL) and used as template for reverse transcription as previously described (Lemaire-Chamley et al. ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids were then purified using the NucleoBond PC 10000 EF Giga kit for endotoxin-free plasmid DNA from Macherey-Nagel. In parallel ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed cells were washed twice with PBS and stained with 20 µg/mL propidium iodide (PI) containing 100 mg/mL RNAse A (740505, MACHEREY-NAGEL) for 15 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultivated in LB medium containing 50 µg/ml Kanamycin and plasmids were isolated using the Nucleo Spin Plasmid Quick Pure protocol (Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... lysates were centrifuged at 21000 g for 10 min and supernatants were transferred to silica membrane spin columns (Macherey-Nagel) and centrifuged at 11000 g for 1 min ...
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bioRxiv - Microbiology 2020Quote: ... Lipids were analysed by Thin Layer Chromatography (TLC) on silica gel-coated plates (G-60, 0.25 mm thickness, Macherey-Nagel) developed with CHCl3/CH3OH/H2O (65:25:4 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Reactions were monitored by thin layer chromatography (TLC) on silica gel (60 F, 0.2 mm, ALUGRAM Sil G/UV254 Macherey-Nagel) by visualization under 254 and/or 365 nm lamp ...
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bioRxiv - Evolutionary Biology 2021Quote: ... isolation of vRNA from 150 µL cell-free supernatants was carried out with the NucleoSpin RNA virus kit (Macherey-Nagel, 740956) as described in the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Plates were scraped with 30-50 mL Luria-Bertani (LB) broth and plasmids were purified with Endotoxin-Free MaxiPrep kit (Macherey-Nagel). Precipitated DNA was resuspended in 1 mL water and the concentration was measured at Nanodrop ...
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bioRxiv - Biophysics 2022Quote: ... and 6 µg total endotoxin free plasmid DNA (4 µg HaloTag-KIF5B1-560 plasmid DNA + 2 µg of α-tubulin-GFP) isolated using the NucleoBond Xtra Midi Plus EF kit (MACHEREY-NAGEL GmbH & Co ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Solid phase extraction was carried out using Chromabond SiOH cartridges filled with 2 g of unmodified silica gel (Macherey-Nagel, Germany). Chemicals ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions were monitored by analytical thin-layer chromatography (TLC) on silica gel plates (Macherey-Nagel, ALUGRAM® Xtra SIL G/UV254) and either visualized by UV light (254 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were monitored by analytical thin layer chromatography (TLC) carried out on ALUGRAM Xtra SIL G/UV254 silica gel plates (Macherey-Nagel). Compounds were visualized with a UV lamp (λ = 254 nm ...
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bioRxiv - Biochemistry 2024Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µg of ground mycelium was used for extraction of high molecular weight DNA with the NucleoBond HMW-Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble fraction containing His6-SUMO tagged Hsf1 was incubated for 25 min at 4°C with 0.4 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was cleared from cell debris by centrifugation at 35.000 g for 30 min and incubated with Ni-IDA matrix (Protino; Macherey-Nagel, Düren, Germany) for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... The lysed culture was centrifuged at 46,000 x g for 1 hour and the supernatant was loaded onto a 1-ml Protino® Ni-NTA column (Macherey-Nagel). The protein was eluted with buffer A + 300 mM imidazole and protein-containing fractions were pooled and dialyzed overnight in buffer A with TEV protease (1/10 w/w ratio ...
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bioRxiv - Microbiology 2022Quote: ... the cultures centrifuged (15 min at 2700×g) and filtered through a 0.2 μm membrane (Chromafil® Xtra PET 20/25, Macherey-Nagel, Germany) were analyzed using a Shimadzu high-performance liquid chromatograph (HPLC ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted in duplicate from ∼1 g of young leaf tissue using the Nucleobond HMW DNA Extraction kit (Macherey-Nagel, Germany). HMW DNA purity and concentration was assessed by Nanodrop and Qubit ...
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bioRxiv - Biochemistry 2020Quote: ... 4°C for 45 min) and the supernatant was incubated for 25 min at 4°C with 2 g Protino Ni-IDA resin (Macherey-Nagel, Düren, Germany) in a rotation shaker ...
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bioRxiv - Genomics 2024Quote: DNA was extracted using either the OmniPrep Genomic DNA Purification Kit (G Biosciences, St. Louis, MO, USA) or the Nucleospin Plant II midi DNA Extraction Kit (Macherey-Nagel, Düren, Germany). DNA quality was assessed using a Qubit fluorometer (Themo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... The mixture was dried under a stream of N2 (g) and samples were resuspended in methanol (140 μL) and transferred into a running vial (Macherey-Nagel, Hoerdt, France). The mixture was then dried and resuspended in methanol (10 µL ...
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bioRxiv - Microbiology 2024Quote: ... The organic fractions were dried and redissolved in 40 µL CHCl3/methanol (80:20) prior to analysis by thin layer chromatography on 0.2 mm silica plates (Macherey-Nagel Xtra SIL G) developed with CHCl3/methanol/acetic acid (80:15:8 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we measured the chemical oxygen demand (COD) using NANOCOLOR COD tube tests (detection range 1-15 g/l by Macherey-Nagel (ref: 985 038), see (32 ...