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Citations for Macherey-Nagel GmbH :
101 - 150 of 196 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... the lysate was treated with 2 µg/µl (w/v) RNAse A (Macherey-Nagel). Genomic DNA of L ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nerve chain and ovaries of each female was extracted using standard protocols (DNA used for ONT sequencing: Macherey-Nagel NucleoBond HMW DNA kit ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Reactions were monitored by thin layer chromatography (Macherey-nagel ALUGRAM SIL G/UV254) by visualisation under 254 nm lamp ...
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bioRxiv - Physiology 2023Quote: ... RNA was extracted from 32 samples of male visceral adipose tissue using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The samples were transferred to tubes with 1.4 mm ceramic beads (Omni International) ...
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bioRxiv - Plant Biology 2023Quote: ... Protein and mRNA were extracted from the same leaf sample (NucleoSpin RNA/Protein kit, REF740933, Macherey-Nagel GmbH & Co., Düren, Germany).
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bioRxiv - Cell Biology 2020Quote: ... Amplified plasmids were isolated using an endotoxin-free plasmid isolation kit (Macherey-Nagel, Germany).
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bioRxiv - Molecular Biology 2023Quote: All plasmids were purified using the Endotoxin-free plasmid DNA purification kit (Macherey-Nagel), the total volume was adjusted to 10% (ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and plasmid DNA was extracted with a Maxiprep endotoxin-free kit (Macherey-Nagel, 740424.10).
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bioRxiv - Molecular Biology 2023Quote: ... and endotoxin-free plasmid DNA was isolated using anion exchange columns (NucleoBond, Macherey-Nagel).
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bioRxiv - Neuroscience 2024Quote: ... and purified using an endotoxin-free midiprep kit (NucleoBond Xtra Midi EF, Macherey-Nagel). Purified plasmid DNA was diluted to a final concentration of 135-150 ng/µL in a solution containing (in mM) ...
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bioRxiv - Microbiology 2023Quote: ... plasmid Midi Preps were prepared using NucleoBond Xtra Midi Endotoxin-free Kit (Macherey-Nagel).
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bioRxiv - Cancer Biology 2023Quote: ... coli under chloramphenicol selection and purified with the Endotoxin-Free MaxiPrep kit (Macherey-Nagel).
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bioRxiv - Microbiology 2023Quote: ... one of which was treated with 20 µg/mL of Proteinase K (740506.75, Macherey-Nagel), and incubated at 37°C for 15 min ...
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bioRxiv - Biophysics 2024Quote: ... One tube was treated with 20 µg/mL of proteinase K (Macherey-Nagel cat #740506). Proteinase K and untreated GPMVs were incubated for 45 min at 37° C ...
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bioRxiv - Biophysics 2023Quote: ... The 600 bp DNA was extracted from the gel using a standard NucleoSpin Gel and PCR cleanup (Macherey-Nagel GmbH). The fluorescence of the doubly labelled 600 bp band was visualized using a Typhoon 9400 scanner (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... equipped with a flame ionization detector (FID) and an Optima-s-3 GC capillary column (30 m x 0.25 mm x 0.25 µm - Macherey-Nagel GmbH & Co, Germany). Nitrogen (200 kPa ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The resulting powder was used for DNA extraction using the NucleoSpin® Tissue kit following manufacturer instructions (MACHEREY-NAGEL GmbH & Co. KG). DNA quantity and purity were checked with a Nanodrop One spectrophotometer (Thermo Scientific ...
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bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... the cultured alone C4-2B cells or the mixed co-cultured C4-2B/MC3T3-E1 were harvested for DNA extraction using the NucleoSpin Tissue kit (Macherey-Nagel Inc.). Primers against the nuclear and mitochondrial genomes were used for qRT-PCR (sequences provided in Supplementary Table S2 ...
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bioRxiv - Evolutionary Biology 2023Quote: DNA was extracted from 8–12 mg of dried tissue using the NucleoSpin 96 Plant II kit (Macherey-Nagel GmbH & Co. KG) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... about 1×103 – 1×104 cells were subjected to total RNA isolation using NucleoSpin RNA Plus XS (MACHEREY-NAGEL GmbH & Co. KG). The first-strand cDNA was synthesized by SuperScript III (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from six-day-old seedlings (approximately 20 seedlings per genotype) using the NucleoSpin RNA plant kit (Macherey-Nagel CO.). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were prepared using the Nucleobond Xtra Midi Endotoxin Free DNA preparation kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Biophysics 2023Quote: ... Clean up of the PCR products was done using a standard cleanup protocol (NucleoSpin Gel and PCR cleanup, Macherey-Nagel GmbH). 2 ...
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bioRxiv - Physiology 2024Quote: ... standard incubation control and after 1,2,4 h at hypoxic conditions) was extracted using the NucleSpin RNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... the aqueous phase was transferred to a RNA extraction column and processed according to the manufacturer’s instructions (NucleoSpin RNA; Macherey-Nagel GmbH & Co, Germany). cDNA was synthesized by reverse transcription of 500ng of total RNA using either both oligo(dT)18 (0.25µg ...
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bioRxiv - Molecular Biology 2022Quote: RNA from cell culture supernatant after CPZ/PCZ treatment (mentioned above) was isolated using viral RNA isolation kit (MACHEREY-NAGEL GmbH & Co. KG). Real-time quantitative RT-PCR was performed in Roche LightCycler 480 using commercial kits ...
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bioRxiv - Cell Biology 2023Quote: ... equipped with C18 trap column packed in-house (1.5 cm, 360-μm outer diameter, 150-μm inner diameter, Nucleosil 100-5 C18; MACHEREY-NAGEL, GmbH & Co. KG) and an analytical C18 capillary self-made column (30 cm ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... Correct clones were determined by sequencing and cultured for subsequent plasmid preparation using NucleoBond Xtra Midi/Maxi kits (Macherey-Nagel GmbH & Co. KG). For scaled CFPS reactions ...
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Chromosome-level assembly of Cucumis sativus cv. ‘Tokiwa’ as a reference genome of Japanese cucumberbioRxiv - Genomics 2024Quote: We also extracted RNA from the leaves for gene annotation used by NucleoSpin RNA Plant kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany).
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bioRxiv - Microbiology 2023Quote: ... TLC analysis was performed on silica gel plates (Sil G/UV254, 0.20 mm, Macherey-Nagel). The Biotage Isolera One Flash Chromatography system was used for flash chromatography and performed on silica gel 60 (Merck ...
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bioRxiv - Developmental Biology 2021Quote: ... The resulting PCR product was purified using a standard PCR purification kit (NucleoSpin® Gel and PCR Clean-up kit, Macherey-Nagel) and used as a template for the second PCR that used the gene-specific forward primer and a 3’ T7 universal reverse primer targeting the forward linker sequence for the antisense probe ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... bacteria from the cultures used for infection were immediately pelleted by centrifugation and resuspended in 8 mL of RES-EF of the NucleoBond Xtra Midi EF kit prior to proceeding with the standard kit protocol (Macherey-Nagel 740420.50). Precipitated vectors were resuspended in 120 µL of water.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... pBDNF was purified using the endotoxin-free plasmid purification NucleoBond®Xtra system (Macherey-Nagel, Germany). The final concentration was measured by OD260 using an UV-visible spectrophotometer (NanoDrop™ ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid DNA was isolated via the endotoxin-free NucleoBond Xtra Midi Plus EF kit (Macherey-Nagel). For transfection ...
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bioRxiv - Synthetic Biology 2022Quote: ... DNA for cell-free reactions was prepared from overnight bacterial cultures using Maxiprep kits (Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... The product of barcoding qPCR was cleaned by gel extraction using NucleoSpin Gel and PCR clean-up kit (MACHEREY-NAGEL, GmbH & Co, KG, Germany), after 1% AGE electrophoresis run for 1 hour at 100 V ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from a total of 24 samples of male brain (four vgll3*EE and four vgll3*LL individuals for each of the three time points) using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The collected samples were transferred to tubes containing 1.4 mm ceramic beads from Omni International ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL was applied on a TLC plate (ALUGRAM® Xtra SIL G UV254, Macherey-Nagel). For the mobile phase CHCl3/MeOH in a ratio of 9:5 (v/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... containing 10 nM internal standard, and 100 µL were transferred into mass spectrometry vials (Wicom, Heppenheim, Germany) with inserts (Macherey-Nagel, Düren, Germany) for carprofen quantification ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were maintained in Escherichia coli and purified with a standard plasmid midi-prep kit (NucleoBond® Xtra Midi, MACHEREY-NAGEL, DE).
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Neuroscience 2023Quote: ... Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US).
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bioRxiv - Microbiology 2024Quote: DNA plasmids were amplified from DH5α cultures and purified using endotoxin-free DNA extraction kits (Macherey-Nagel) as previously described (28) ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were resuspended in PBS with 20 µg/ml PI and 0.2 mg/mL RNAse A (Macherey-Nagel) and incubated at RT for 30 min before analysis on a FACSCalibur (Beckton Dickinson) ...
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bioRxiv - Plant Biology 2024Quote: The tRNA fraction was isolated from 17 µg of total RNA using Nucleobond AX-20 columns (Macherey-Nagel) following a rescaled protocol based on (28) ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Developmental Biology 2022Quote: ... All plasmids were cloned into the DH5a strain (Enzynomics) and purified with a NucleoBond Xtra Midi Plus kit (MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... Japan) equipped with an analytical CN-RP column (NUCLEODUR 100-5, CN-RP, 150 × 4.6, 5 µm column; Macherey-Nagel GmbH & Co. KG, Düren, Germany) and a UV-diode-array detector ...