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Citations for Macherey-Nagel GmbH :
1901 - 1950 of 2266 citations for Mouse Tryptophan 2 3 Dioxygenase TDO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... MDDCs were lysed and total RNA was extracted using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel, 740955) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Products from four PCR reactions were pooled and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel #740609). Equal amounts of products were mixed and sequenced on Illumina NextSeq 500 instruments using the NextSeq high-output kit (75 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... the genomic DNA of the expanded clones was extracted with a kit according to the manufacturer’s protocol (Nucleospin Tissue, Macherey-Nagel) to perform PCR genotyping ...
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bioRxiv - Plant Biology 2024Quote: ... and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel). All amplicons obtained from a single biological replicate were pooled in equimolar amounts and a total of 100 ng were used for library preparation following the modified Illumina Nextera Flex library preparation protocol described before (28 ...
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted by phenol-chloroform extraction followed by column clean-up using NucleoSpin® DNA Plant II kit (Macherey-Nagel). Clustering and DNA sequencing of wild-type ...
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bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in RNA lysis buffer and RNA was isolated using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel). For some experiments ...
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bioRxiv - Genetics 2021Quote: ... DNA from three female and three male fifth instar larvae was isolated individually using the NucleoSpin DNA Insect kit (Macherey-Nagel). We observed that the primers Masc_F1 and Masc_R1 targeting EkMasc and EkMascB consistently showed off-target amplification in female samples ...
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bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flowers and whole chili pepper fruits at different developmental stages using a NucleoSpin(tm) RNA Plant kit (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... and used for DNA extraction using the NucleoSpin Plant midi DNA extraction kit following the manufacture’s protocol (Macherey-Nagel, Düren, Germany). The genomic DNA was quality controlled using a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1.7 Gb paired-end reads for the RS_LinB were obtained from Red Sea samples preserved in CHAOS buffer (Flot 2007) and extracted using the DNA NucleoSpin kit (Macherey-Nagel) following the protocol recommended by the company ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from ~100 mg of feces with the use of a Nucleospin DNA Stool Kit (740472, Macherey-Nagel) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Systems Biology 2019Quote: ... Histone-DNA complexes were isolated from insoluble nuclear chromatin by centrifugation and DNA was extracted with a NucleoSpin PCR Clean-up kit (Macherey-Nagel). For CUT&RUN quantitative PCR ...
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bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
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bioRxiv - Bioengineering 2019Quote: ... PCR products were analysed by agarose gel electrophoresis and gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Constructs were transformed into E ...
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Dual functionality of the TasA amyloid protein in Bacillus physiology and fitness on the phylloplanebioRxiv - Microbiology 2019Quote: ... DNA removal was carried out via in-column treatment with the rDNAse included in the Nucleo-Spin RNA Plant Kit (Macherey-Nagel) following the instructions of the manufacturer ...
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bioRxiv - Genetics 2020Quote: ... PCR reactions were separated on agarose gels and PCR fragments isolated using a Nucleo-Spin Gel and PCR Clean-up kit (Macherey-Nagel). Sanger sequencing of PMEL and KIT ...
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bioRxiv - Microbiology 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The products were isolated by cutting the band from an agarose gel and clean-up with the NucleoSpin Extract II kit (Macherey-Nagel). Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Large quantities of plasmid DNAs for transfection were prepared from the pellets of overnight cultures of pNC-HN8 and pNC-HN8GGm clones using the NucleoBond Xtra Midi kit (Macherey-Nagel) as instructed by the manufacturer.
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bioRxiv - Biochemistry 2020Quote: ... Contaminating genomic DNA was removed using DNase and the total RNA sample was further purified with a NucleoSpin RNA Clean-up kit (Macherey-Nagel). Sequencing was then performed on an Illumina HiSeq 1500 instrument as 101 bp single read runs.
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bioRxiv - Bioengineering 2021Quote: Cell pellets were washed and lysed using RA1 RNA extraction buffer and purified using the NucleoSpin RNA kit (MAcherey-Nagel; 740955.250) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
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bioRxiv - Plant Biology 2021Quote: ... Total RNA from leaves of four-week-old Arabidopsis plants was extracted using the NucleoSpin RNA plant extraction kit (Macherey-Nagel) and treated with rDNase according to the manufacturer’s extraction protocol ...
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bioRxiv - Genomics 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin-RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were collected into the RA1 lysis buffer and extracted using the Nucleospin RNA Plus Extraction kit (740984, Macherey-Nagel).
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bioRxiv - Plant Biology 2022Quote: 100mg of leaf tissues from the second to fourth fully emerged leaf from the apical bud were used for DNA extraction using the NucleoSpin Plant II kit (Macherey-Nagel). Leaf tissues were disrupted with 400µL PL1 from the kit using BeadBeater (BioSpec ...
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bioRxiv - Plant Biology 2022Quote: 30mg of leaf tissues from the eighth fully emerged leaf from the apical bud were used for RNA extraction using the NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel). Leaf samples were sampled from the plants that were at the end of 8 weeks after being transplanted to the 2-gallon pot ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
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bioRxiv - Microbiology 2022Quote: ... the band corresponding to the amplicon was excised from the gel and purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... and RNA was extracted by phenol-chloroform and cleaned up using the NucleoSpin® RNA XS extraction kit (Macherey-Nagel, Germany). Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA from each sample was isolated using the NucleoSpin® RNA isolation kit (Macherey-Nagel, Hoerdt Cedex, France) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... the genomic DNA from the edited cells was isolated after the transduction for eight days using a DNA isolation kit (NucleoSpinBlood - Macherey-Nagel). The target region for base editing was amplified using the respective primers (Sup Tables 2 and 3) ...
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bioRxiv - Plant Biology 2021Quote: Ribonucleic acid (RNA) samples were isolated from seeds and leaves using the NucleoSpin® RNA Plant kit (Macherey-Nagel, Düren, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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bioRxiv - Immunology 2020Quote: Total cellular RNA (containing viral genomic RNA) was extracted 48 h after transfection using NucleoSpin RNA Plus kit (Macherey-Nagel, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments were purified by agarose gel electrophoresis followed by extraction from an excised gel slice using a NucleoSpin Extract II kit (Macherey-Nagel). Hybridization probes were labeled with α[32P]dCTP by random priming (Multiprime DNA labeling kit ...
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bioRxiv - Immunology 2021Quote: Clones whose culture had proven successful (IgG concentration ≥ 1 μg/mL at day 21-25) were selected and extracted using the NucleoSpin96 RNA extraction kit (Macherey-Nagel) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Both PCR products were excised from a 1% agarose gel and extracted using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) and used as templates for In-Fusion HD cloning (Takara) ...
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bioRxiv - Genetics 2022Quote: ... cells were scraped from plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel). The resulting plasmid DNA was used for yeast transformation.
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bioRxiv - Genetics 2022Quote: ... the cells were scraped from the plates using sterile water and plasmid DNA was extracted from 400 OD600nm units (Nucleobond Xtra Midiprep Kit, Macherey-Nagel).
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bioRxiv - Evolutionary Biology 2022Quote: ... PCR products with multiple bands were excised and purified from gel using the Nucleospin Gel and PCR Clean-up kit from Macherey-Nagel, or cloned using the NEB® PCR cloning kit (New Englands BioLabs ...