Labshake search
Citations for Macherey-Nagel GmbH :
1901 - 1950 of 2292 citations for Bicinchoninic Acid BCA Protein Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... Products from four PCR reactions were pooled and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel #740609). Equal amounts of products were mixed and sequenced on Illumina NextSeq 500 instruments using the NextSeq high-output kit (75 cycles ...
-
bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
-
bioRxiv - Synthetic Biology 2024Quote: ... the genomic DNA of the expanded clones was extracted with a kit according to the manufacturer’s protocol (Nucleospin Tissue, Macherey-Nagel) to perform PCR genotyping ...
-
bioRxiv - Plant Biology 2024Quote: ... and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel). All amplicons obtained from a single biological replicate were pooled in equimolar amounts and a total of 100 ng were used for library preparation following the modified Illumina Nextera Flex library preparation protocol described before (28 ...
-
bioRxiv - Microbiology 2024Quote: Genomic DNA was extracted from 200 μL of pelleted red blood cells diluted 1:1 in PBS1X using the DNA Nucleospin blood extraction kit following the manufacturer’s instructions (Macherey-Nagel). The extracted DNA was then stored at -20°C for later use.
-
bioRxiv - Microbiology 2024Quote: ... Microbial DNA was obtained from 200 mg of rhizosphere material using the Nucleo Spin® Soil kit (Macherey-Nagel, Germany). Quantitative determination of the concentration (ng/μl ...
-
bioRxiv - Neuroscience 2024Quote: 8-10 organoids per extraction were pooled and RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) following the manufacturer9s instructions and cDNA synthesis was performed using Superscript# III First- Strand Synthesis SuperMix (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2024Quote: ... We purified the resulting fragment from a 2% agarose gel using the NucleoSpin Gel and PCR Purification Kit (Macherey-Nagel). We amplified the 1483bp RTFDC1 promoter sequence off of a promoter plasmid (Genecopoeia HPRM41362-PF02 ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA from tissues or cells was isolated using an RNA isolation kit (NucleoSpin RNA, Macherey-Nagel GmbH & co.KG, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... Aliquots (4 - 6 mL) of overnight cultures were used for recipient plasmid extraction using the NucleoSpin plasmid kit (Macherey-Nagel).
-
bioRxiv - Immunology 2024Quote: ... We then stored the pellet at -20 °C for RNA isolation using the NucleoSpin RNA kit (MACHEREY-NAGEL, Düren, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The resulting PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH, Cat. # 740609.50), following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... The rest of the PCRs were purified with the NucleoSpin Gel and PCR Clean-up XS kit (#740611.50, Macherey-Nagel) for long-read amplicon sequencing using Oxford Nanopore Technology (via Plasmidsaurus).
-
bioRxiv - Pharmacology and Toxicology 2024Quote: Total RNAs were extracted from about 6 ×106 HepaRG cells using a Nucleospin RNA isolation Kit (Macherey-Nagel, Hoert, France) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2024Quote: ... Each of the three PCR reactions was column purified with a Nucleospin PCR clean-up kit according to the manufacturer’s protocol (Macherey-Nagel). The concentration of dsDNA from each PCR reaction was determined on a microvolume UV-vis spectrometer (DeNovix).
-
bioRxiv - Molecular Biology 2024Quote: DNA was extracted from two or three legs per specimen using a NucleoSpin® Tissue kit (MACHEREY-NAGEL Düren, Germany). The isolated DNA was resuspended in Tris-HCl buffer (pH 7.5 ...
-
A comparative analysis between two flax varieties indicates lignan-mediated salt stress adaptivenessbioRxiv - Plant Biology 2024Quote: Total RNA was isolated from 100 mg of shoot sample using the NucleoSpin RNA Plant mini kit (Macherey-Nagel, Germany) following the kit-specified protocol ...
-
bioRxiv - Immunology 2024Quote: ... PCR was migrated in a 1.3% agarose gel and amplicons were cut and purified using a gel and PCR clean-up kit (Macherey-Nagel) for further DNA sequencing ...
-
bioRxiv - Cancer Biology 2024Quote: RNA from human prostate cancer cell lines was extracted using NucleoSpin® RNA isolation kit from Macherey-Nagel (Ref: 740955.240C), following the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We grew plants in the greenhouse and extracted DNA from young leaf tissue using NucleoSpin Plant II kit (Macherey-Nagel) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... DNA was extracted by phenol-chloroform extraction followed by column clean-up using NucleoSpin® DNA Plant II kit (Macherey-Nagel). Clustering and DNA sequencing of wild-type ...
-
bioRxiv - Molecular Biology 2020Quote: ... An amount of 0.25 g (3.6 g TSS L−1) activated sludge was processed to extract intracellular DNA using a NucleoSpin® Soil kit (Macherey-Nagel) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... Tissue homogenates were resuspended in RNA lysis buffer and RNA was isolated using the NucleoSpin RNA Plus RNA isolation kit (Macherey-Nagel). For some experiments ...
-
bioRxiv - Genetics 2021Quote: ... DNA from three female and three male fifth instar larvae was isolated individually using the NucleoSpin DNA Insect kit (Macherey-Nagel). We observed that the primers Masc_F1 and Masc_R1 targeting EkMasc and EkMascB consistently showed off-target amplification in female samples ...
-
bioRxiv - Molecular Biology 2021Quote: ... the DNA eluates were de-cross-linked at 65°C overnight with shaking at 900 rpm and purified by NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2020Quote: Total RNA was extracted from flowers and whole chili pepper fruits at different developmental stages using a NucleoSpin(tm) RNA Plant kit (MACHEREY-NAGEL) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
-
bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
-
bioRxiv - Genomics 2020Quote: ... and used for DNA extraction using the NucleoSpin Plant midi DNA extraction kit following the manufacture’s protocol (Macherey-Nagel, Düren, Germany). The genomic DNA was quality controlled using a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2020Quote: ... for 30 min at 37 °C and gel purified with a NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). The oligo-pool amplicons were assembled into the linearized Lenti-gRNA-Puro plasmid using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
-
bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... The next day bacteria were floated off the plates with liquid LB medium and plasmid DNA was isolated using the NucleoBond Xtra Maxi Kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... The amplified products were identified by gel electrophoresis and purified using Nucleospin PCR and Gel Clean Up Kit (Macherey-Nagel # 740609.5). Alternatively ...
-
bioRxiv - Genetics 2020Quote: ... PCR reactions were separated on agarose gels and PCR fragments isolated using a Nucleo-Spin Gel and PCR Clean-up kit (Macherey-Nagel). Sanger sequencing of PMEL and KIT ...
-
bioRxiv - Developmental Biology 2020Quote: ... DNA from a male wild type blood sample (2 ml) was purified with the NucleoBand®HMW DNA Kit (Macherey-Nagel). To eliminate fragments below 40 kb the Short Read Elimination Kit XL (Circulomics ...
-
bioRxiv - Microbiology 2021Quote: ... RNA was isolated from aliquots of supernatant containing selected viral populations or individual plaque purified variants using NucleoSpin 96 Virus Core Kit (Macherey-Nagel). The purified RNA was subjected to reverse transcription using random hexamer primers and SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... The products were isolated by cutting the band from an agarose gel and clean-up with the NucleoSpin Extract II kit (Macherey-Nagel). Labelled DNA was generated using the same PCR program with the DIG Easy Hyb (Roche ...
-
bioRxiv - Biochemistry 2020Quote: ... Large quantities of plasmid DNAs for transfection were prepared from the pellets of overnight cultures of pNC-HN8 and pNC-HN8GGm clones using the NucleoBond Xtra Midi kit (Macherey-Nagel) as instructed by the manufacturer.
-
bioRxiv - Biochemistry 2020Quote: ... Contaminating genomic DNA was removed using DNase and the total RNA sample was further purified with a NucleoSpin RNA Clean-up kit (Macherey-Nagel). Sequencing was then performed on an Illumina HiSeq 1500 instrument as 101 bp single read runs.
-
bioRxiv - Bioengineering 2021Quote: Cell pellets were washed and lysed using RA1 RNA extraction buffer and purified using the NucleoSpin RNA kit (MAcherey-Nagel; 740955.250) following the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2021Quote: ... the amplified plasmid library was extracted from bacterial cells using 4 reactions of Midiprep kit (Macherey-Nagel NucleoBond Xtra Midi Plus).
-
bioRxiv - Plant Biology 2021Quote: ... Total RNA from leaves of four-week-old Arabidopsis plants was extracted using the NucleoSpin RNA plant extraction kit (Macherey-Nagel) and treated with rDNase according to the manufacturer’s extraction protocol ...
-
bioRxiv - Genomics 2020Quote: Cells were rinsed with phosphate buffer saline (PBS) before to be homogenized in RNA lysis buffer (NucleoSpin-RNA Kit, Macherey-Nagel), supplemented with 1% β-Mercaptoethanol ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were collected into the RA1 lysis buffer and extracted using the Nucleospin RNA Plus Extraction kit (740984, Macherey-Nagel).
-
bioRxiv - Plant Biology 2022Quote: 100mg of leaf tissues from the second to fourth fully emerged leaf from the apical bud were used for DNA extraction using the NucleoSpin Plant II kit (Macherey-Nagel). Leaf tissues were disrupted with 400µL PL1 from the kit using BeadBeater (BioSpec ...
-
bioRxiv - Plant Biology 2022Quote: 30mg of leaf tissues from the eighth fully emerged leaf from the apical bud were used for RNA extraction using the NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel). Leaf samples were sampled from the plants that were at the end of 8 weeks after being transplanted to the 2-gallon pot ...
-
bioRxiv - Cell Biology 2022Quote: ... Plasmids were propagated in Eschericia coli DH10B cells using standard procedures and purified using NucleoBond Xtra Midi EF Kit (Macherey-Nagel).
-
bioRxiv - Microbiology 2022Quote: ... the band corresponding to the amplicon was excised from the gel and purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following manufacturer’s instructions ...