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Citations for Macherey-Nagel GmbH :
51 - 100 of 129 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... genomic DNA (gDNA) was isolated using the XL Maxi NucleoSpin Blood kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... falciparum positive DBS was extracted with the NucleoMag Blood 200 μL kit (Macherey-Nagel) using an optimized protocol for DNA extraction from whole 50 μL DBS ...
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bioRxiv - Microbiology 2024Quote: ... C3 and C5 clones using NucleoSpin Blood QuickPure kit (Macherey-Nagel GmbH, Düren, Germany). The library for MinION was constructed with a Ligation Sequencing Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA (gDNA) was exptracted using the NucleoSpin Blood XL kit (Macherey-Nagel, # 740950.50). The gDNA was amplified according to Broad Genome Perturbation Portal protocols and single end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... treatment with 100µg/ml RNase A (37° for 15 minutes) and 50µg/ml proteinase K and purification using the PCR cleanup kit (Macherey-Nagel).
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bioRxiv - Genomics 2020Quote: ... DNA was extracted from 1 ml of crude homogenate containing 6 μl of proteinase K (22 mg/ml, Macherey-Nagel), incubated overnight at 55°C.
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bioRxiv - Genomics 2021Quote: ... Genomic DNA was isolated using a NucleoSpin Blood DNA extraction kit (Macherey-Nagel, Bethlehem PA). The sgRNA-containing region was PCR-amplified with NEBNext Ultra II Q5 MasterMix (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was purified using Macherey-Nagel NucleoSpin Blood XL Maxi Kit (740950.50, Macherey-Nagel) and prepared as previously described [Kampmann et al 2014] with modifications ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from cell pellets using the NucleoSpin Blood XL Kit (Macherey-Nagel). The sgRNA sequence was amplified by PCR with sufficient gDNA to maintain representation ...
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bioRxiv - Genetics 2024Quote: ... following one stimulation (Acute) and after six stimulations (Chronic) using NucleoSpin Blood kits (Macherey-Nagel) (Extended Data Fig ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA was eluted from HisPur Cobalt Resin by incubating samples in a final volume of 100 µl of PBS with 0.4 mg/ml of Proteinase K (Macherey-Nagel, #740506) and incubated for 2 h at 65°C with shaking at 800 rpm ...
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bioRxiv - Systems Biology 2022Quote: ... Genomic DNA from the cell pellets was purified using the NucleoSpin blood L kits (Macherey-Nagel) according to the manufacturer’s instructions and was quantified using PicoGreen dsDNA assay kits (ThermoFisher).
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bioRxiv - Genomics 2021Quote: ... genomic DNA was isolated using a Macherey-Nagel Blood L kit (Macherey-Nagel; Cat. No. 740954.20). sgRNA-encoding regions were amplified and sequenced on an Illumina HiSeq- 4000 as previously described4.
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated using a Macherey-Nagel Nucleospin Blood kit (Macherey-Nagel; Cat. No. 740951.50) or Macherey-Nagel Nucleospin Blood L kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated using a Macherey-Nagel Blood L kit (Macherey-Nagel; Cat. No. 740954.20) and sgRNA sequences were amplified and sequenced on an Illumina MiSeq V3.
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bioRxiv - Cell Biology 2021Quote: ... Eluates were digested with proteinase K and RNAse A and DNA fragments were purified using a PCR purification kit (Macherey-Nagel, #740609).
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bioRxiv - Microbiology 2020Quote: DNA was extracted from 100 μL blood using the Genomic DNA Extraction kit (Macherey-Nagel, Düren, Germany) and eluted in an equivalent volume of elution buffer ...
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bioRxiv - Physiology 2020Quote: ... Genomic DNA from 32 MPM cell lines was extracted with Nucleospin Blood kit (Macherey-Nagel, Düren, Germany) and 500 ng were hybridized to Affymetrix CytoScanHD Arrays (Affymetrix ...
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bioRxiv - Immunology 2020Quote: ... RNA was isolated from whole blood samples using the NucleoSpin 96 RNA tissue kit protocol (Macherey-Nagel) with some modifications as previously described15.
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bioRxiv - Genomics 2024Quote: Foot tissue was gently dissolved by addition of 1 µL of 50 µg/mL Proteinase K (Macherey-Nagel GmbH & Co. KG, Duren, Germany) to 1 ml TNE8U buffer containing the tissue and incubating at 55°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... The gDNA was isolated from the transgenic parasite lines using the NucleoSpin Blood Kit (Macherey-Nagel; Dueren, DE) following the manufacturer’s protocol and used as template to verify the successful vector integration by diagnostic PCR (for primer sequence ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
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bioRxiv - Genomics 2023Quote: High molecular weight DNA was extracted from blood cells using a NucleoBond AXG column (Macherey-Nagel, Düren, Germany), which was followed by purification with phenol-chloroform ...
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bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
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bioRxiv - Genetics 2022Quote: RNA extraction was performed from frozen tissue in culture plates (P6 well plate, Falcon®) by using the NucleoSpin RNA kit (Macherey-Nagel). RNA concentration was measured with NanoDrop1000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Immunology 2022Quote: ... RNA was isolated from peripheral blood mononuclear cells (PBMC; NucleoSpin RNA Mini Plus, Macherey-Nagel GmbH & Co.KG, Düren, Germany). For cDNA first strand synthesis RevertAid kit (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: Genomic DNA was isolated from total thymocytes and from sorted T-cell subsets from MCMV-infected and uninfected mice according to the manufacturer’s instructions using the NucleoSpin Blood QuickPure kit (MACHEREY-NAGEL), and stored at −20°C until further analysis.
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bioRxiv - Microbiology 2022Quote: DNA from monolayer cultures as well as from rafts was isolated using the NucleoSpin Blood QuickPure kit (740569.250; Macherey-Nagel) according to the manufacturer’s instructions with some modifications for raft tissues ...
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bioRxiv - Genomics 2024Quote: ... each side of the Amicon filter was rinsed and incubated at 56°C for 1 hour (per side) in 200 µL Buffer T1 and 20 µL Proteinase K from the NucleoSpinl1J Tissue Kit (MACHEREY-NAGEL Inc., Allentown, PA, United States) and DNA extraction proceeded from step 3 of the kit ...
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bioRxiv - Biochemistry 2020Quote: ... Individual quenched timepoints were spotted PEI-TLC plates (Macherey-Nagel) and nucleotides were resolved in 1.5 KH2PO4 pH 3.5 buffer ...
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bioRxiv - Microbiology 2023Quote: ... silica gel G60 plates (10 x 20 cm, Macherey-Nagel) were used to spot samples and lipids were separated with CHCl3/CH3OH (90:10 ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA from a male wild type blood sample (2 ml) was purified with the NucleoBand®HMW DNA Kit (Macherey-Nagel). To eliminate fragments below 40 kb the Short Read Elimination Kit XL (Circulomics ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from blood samples of patients II-8 and IV-1 using the Nucleospin extraction kit (ref 740952-Macherey-Nagel). Subsequently ...
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bioRxiv - Evolutionary Biology 2023Quote: RNA was successfully isolated from blood cells of 34 14-day-old great tits with NucleoSpin RNA Plus Kit (Macherey-Nagel). Packed blood cells (10 µl per sample ...
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bioRxiv - Microbiology 2022Quote: ... Viral genome equivalents (vge) were determined by qPCR of DNA isolated from encapsidated virions isolated with NucleoSpin Blood kit QuickPure (Macherey-Nagel; 740569.250).
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bioRxiv - Microbiology 2022Quote: ... Viral genome equivalents (vge) were determined by real-time quantitative PCR (qPCR) of encapsidated DNA isolated using the NucleoSpin Blood QuickPure (Macherey-Nagel; 740569.250). To generate mutated quasiviruses ...
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bioRxiv - Genomics 2021Quote: Sequencing libraries were prepared from the cells collected at the end of the screens as follows: Genomic DNA was extracted using the NucleoSpin® Blood XL kit (Macherey-Nagel) with a condition of 50e6-100e6 cells per column ...
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bioRxiv - Microbiology 2022Quote: Total RNA was isolated from mock- and KSHV-infected blood or lymphatic ECFCs using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). RNA was further concentrated and purified using the RNA Clean and Concentrator kit (Zymo Research ...
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bioRxiv - Genetics 2024Quote: 200,000 cells were collected on day 14 after crRNA transduction and genomic DNA was isolated using NucleoSpin Blood (Macherey-Nagel, Catalog no. 740951.50). Briefly ...
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bioRxiv - Genetics 2021Quote: ... Vacuum the EconoSpin plate on the vacuum manifold (MACHEREY-NAGEL, Dueren, Germany) until no droplet drop down ...
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bioRxiv - Plant Biology 2020Quote: PCR products were purified by NucleoFast 96 PCR plates (Macherey-Nagel, Germany) and were sequenced directly on both DNA strands on Applied Biosystems (Weiterstadt ...
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bioRxiv - Microbiology 2021Quote: ... The amplicons were then purified using NucleoFast 96 PCR Plate (Macherey-Nagel) coupled to a vacuum pump ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA was isolated from peripheral blood and tail tissues of each chimeric mouse 6 weeks after BM transplantation with NucleoSpin® Tissue Kit (MACHEREY-NAGEL, Duren, Germany). Then ...
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bioRxiv - Biochemistry 2022Quote: ... C18 reversed-phase thin layer chromatography (TLC) plates were obtained from Macherey-Nagel. PEI cellulose TLC plates were obtained from EMD Millipore.
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bioRxiv - Immunology 2023Quote: ... and ADSCs were lysed in the plate using in lysis buffer containing TCEP (Macherey-Nagel). Lysed cells were snap frozen in nitrogen and stored at -80°C until use.
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bioRxiv - Microbiology 2023Quote: ... TLC analysis was performed on silica gel plates (Sil G/UV254, 0.20 mm, Macherey-Nagel). The Biotage Isolera One Flash Chromatography system was used for flash chromatography and performed on silica gel 60 (Merck ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty percent of the reaction mixture was spotted onto a polyethyleneimine cellulose plate (MACHEREY-NAGEL) that had been pre-run with water for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μL was applied on a TLC plate (ALUGRAM® Xtra SIL G UV254, Macherey-Nagel). For the mobile phase CHCl3/MeOH in a ratio of 9:5 (v/v ...
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bioRxiv - Microbiology 2021Quote: ... Reaction products were visualized by thin layer chromatography (TLC) in PEI cellulose TLC plates (Macherey-Nagel) and separated using 0.75 M KH2PO4 ...