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Citations for Macherey-Nagel GmbH :
601 - 650 of 778 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... Bacteria were collected and the plasmid DNA was purified using NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, cat. 740410.50). The transformation efficiency was estimated by plating 1/10000 of the transformation reaction onto 15 cm LB-agar plate with 100 µg/ml ampicillin and counting number of clones after incubation overnight at 37°C.
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bioRxiv - Cancer Biology 2019Quote: ... Total RNA were purified after 72 hours incubation with a Nucloespin RNA purification kit (Macherey-Nagel cat. no. 740955) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2019Quote: ... purified according to the instructions of the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel GmbH, Düren, Germany) and cloned into the pET22b(+ ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were purified using a NucleoSpin Gel and a PCR Clean-up Kit (Macherey-Nagel, Duren, Germany) and then sequenced directly by Sanger sequencing using the same PCR primers ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitated DNA was purified with NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... ChIP-DNA and input DNA were purified using the NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel). The DNA concentration of the input DNA was determined using the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... The amplified rDNA gene fragments were purified using nucleobond AX 100 columns for Miniprep System (Macherey-Nagel, ref 740521.100). The rDNA sequences were then labeled with Digoxigenin-11-dUTP by nick translation according to the manufacturer’s protocol (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... All plasmids were purified from 4 ml bacterial culture using either NucleoSpin® Plasmid (REF 740588.50, Macherey-Nagel®) or PureLink™ HiPure Plasmid Miniprep Kit (Art ...
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were purified using Macherey-Nagel Nucleospin® RNA Clean-up kit (Macherey-Nagel, Bethlehem, PA) prior to injections.
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bioRxiv - Microbiology 2022Quote: ... A total of 400 µl of this PCR was run and purified using column-based clean-up (Macherey-Nagel).
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bioRxiv - Immunology 2021Quote: RNA of polyclonally activated CD8+ T cells from C57BL6/N mice was purified using NucleoSpin RNA kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... Poly-His tagged recombinant proteins were purified using a nickel resin (Protino Ni-TED 2000 Packed Columns, Macherey-Nagel) and exchanged to Tris buffer 50 mM pH 8.0 in Sephadex™ G-25 M resin (PD-10 Columns ...
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bioRxiv - Biochemistry 2021Quote: ... and the GST fusion proteins were purified using Protino gluthathione agarose 4B according to the manufacturer’s instruction (Macherey-Nagel). SPR interaction studies were performed according to previous studies46,85 ...
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bioRxiv - Genomics 2021Quote: ... PCR was performed and the products were purified with a NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL).
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were purified from agarose gels using the NucleoSpin Gel and PCR Clean-up kit from Macherey-Nagel. Genomic DNA extractions from Amycolatopsis and E ...
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bioRxiv - Immunology 2022Quote: ... RNA from dry pellets of Vγ9Vδ2 T lymphocytes was extracted and purified using the NucleoSpin RNA plus isolation kit from Macherey-Nagel. DGE-sequencing was performed by the BIRD core facility and data were analyzed using R programming languages and tools.
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel™, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... the adeD PCR product was additionally gel-purified (NucleoSpin Gel and PCR Clean-Up Kit, Macherey-Nagel, Düren, DE), and 100 ng of purified DNA was subsequently incubated with 0.2 M dATP and 5 U DreamTaq Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Resulting PCR products were purified from 1% agarose gels with NucleoSpin Gel and PCR Clean-Up Kit (Macherey-Nagel) to prevent the production of side products during in vitro transcription ...
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bioRxiv - Molecular Biology 2022Quote: ... All PCR fragments for cloning and plasmids were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and GenElute™ Plasmid Miniprep Kit (Merck) ...
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bioRxiv - Plant Biology 2022Quote: ... Proteins from the soluble fraction were purified by affinity chromatography with Protino Ni-TED PackedColumns2000 (Macherey-Nagel, Düren, Germany) based on the His-tag tail ...
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bioRxiv - Molecular Biology 2022Quote: ... Certain PCR products were purified from the gel using NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel) and cloned individually in a TA-cloning vector called pGEM®-T Easy Vector (Promega ...
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bioRxiv - Bioengineering 2023Quote: ... Library plasmids were harvested from transformed bacteria and purified using the NucleoBond Xtra MIDI plasmid preparation kit (Macherey-Nagel). The AAV library genomes were packaged into 293T cells by transfecting 1 × 107 in ten 15-cm with the library plasmids (containing the cap gene with peptide insertion ...
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bioRxiv - Molecular Biology 2023Quote: ... Five tubes of each amplicon were pooled and DNA purified using NucleoSpin Gel and PCR Clean-Up (Macherey-Nagel). DNA was eluted in 20 µl of Elution Buffer and concentration was measured using Qubit 3.0 fluorometer (Life Technologies).
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bioRxiv - Bioengineering 2022Quote: ... All constructs were verified by sequencing and purified using a NucleoBond Xtra Midi EF kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... and linearized plasmid was purified from the gel using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). In-Fusion reaction was prepared as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... The linearised fragment was then excised and purified using the NucleoSpin Gel and PCR Clean up Kit (Macherey-Nagel). 400nM of the annealed oligos were ligated with lng of digested crRNA backbone using T4 ligase (Promega ...
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bioRxiv - Evolutionary Biology 2023Quote: ... They have been further purified using the Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al. 2011). The extracts were subsequently diluted in water and quantified with a Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
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bioRxiv - Neuroscience 2024Quote: ... the bands of interest were excised and purified with the Gel and PCR clean up (ref 740609, Macherey-Nagel) with a final elution volume of 20 µl ...
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were purified to remove unincorporated nucleotides and primers using NucleoFast® 96 PCR plates (Macherey-Nagel, Oensingen, Switzerland). Details of the annealing temperatures and primers were published previously (Friesen et al ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR product was purified with NucleoSpin Gel and PCR clean-up kit according to manufacturer’s description (Macherey-Nagel, 740609). RF cloning into pET28a was performed with eighteen 3-step cycles with annealing temperature 65°C and extension time 7 minutes ...
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bioRxiv - Genomics 2019Quote: ... PCR products were checked by agarose gel electrophoresis and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). Cycle sequencing reactions were performed using the BigDye 3.1 chemistry ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were then purified using a Millipore NucleoFast 96 PCR kit following the manufacturer’s recommendations (Macherey-Nagel, Düren, Germany) and sequenced using the BigDye Terminator Cycle Sequencing Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... cDNA constructs for human Parkin mutant expression were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: ... and purified directly from the PCR mix or from an agarose gel (Gel and PCR clean-up kit, Macherey-Nagel); the two fragments were fused together thanks to a primer-encoded 20-30bps overlap during a second PCR amplification round and the product cloned into the pEMG suicide vector [20] using EcoRI and BamHI ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 μL water was added and DNA was purified using Nucleospin Gel and PCR Cleanup (Macherey-Nagel, Cat. No 740609.250). Extracted DNA was PCR-amplified to check the insertion of the 3XFLAG tag ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA constructs for mammalian tissue culture (Supplementary Table 1) were amplified in Escherichia coli DH5α and purified using a NucleoBond Xtra Midi kit (#740420.50; Macherey-Nagel). All DNA constructs were verified by DNA sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... guide RNAs and Cas9 were expressed from plasmids that were purified with the NucleoBond® Xtra Midi Kit (Macherey-Nagel). In the remaining mixes ...
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bioRxiv - Immunology 2020Quote: ... Positive clones were identified by colony PCR and the plasmid was purified using NucleoSpin® Plasmid EasyPure kit (Macherey-Nagel). The cloned devil NLRC5 transcript was verified by Sanger sequencing using Big Dye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems (ABI) ...
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bioRxiv - Immunology 2020Quote: ... amplicons were separated by gel electrophoresis and purified using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany). Library quantification and quality control was performed using Qubit (QIAGEN ...
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bioRxiv - Plant Biology 2019Quote: ... DNA from the IP and input fractions was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel). DNA from IP and input were used for library preparation and sequencing by Illumina Hi-Seq (FASTERIS ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were recovered and purified using a NucleoSpin® Gel and PCR Clean-up Kit (Macherey-Nagel, Düren, DE). Concentrations of purified PCR products were determined by absorbance measurements at a wavelength of 260 nm using an infinite F200 pro device (TECAN ...
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bioRxiv - Developmental Biology 2021Quote: ... The DNAs have been extracted on an agarose gel and purified (using the PCR clean-up gel extraction kit #740609.50, Macherey-Nagel) and the sanger sequencings have been performed by Eurofins ...
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bioRxiv - Genetics 2020Quote: ... Obtained libraries were pooled in equimolar amounts and purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel), followed by a second purification step of gel extraction and size exclusion with AMPure XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Biophysics 2022Quote: ... The product was then purified from 1% agarose gel and extracted using the NucleoSpin clean up kit (Macherey-Nagel, 740588.50).