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Citations for Macherey-Nagel GmbH :
301 - 350 of 818 citations for Purified Zika Virus Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... DNA was purified using NucleoSpin PCR clean-up kit (Macherey-Nagel, cat# 740609), dissolved with ddH2O and adjusted to a concentration of 0.6 μM ...
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bioRxiv - Bioengineering 2023Quote: ... Pooled library plasmids were purified using NucleoBond PC 500 Maxi kit (Macherey-Nagel). The same protocol was applied to generate the library for the second round of selection ...
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bioRxiv - Biochemistry 2023Quote: ... The GST fusion proteins were purified with Protino Glutathione Agarose 4B (Macherey-Nagel) and GST was cleaved by human thrombin (Biopharm laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... The ligation was purified by NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) and used for the electroporation of ElectroMAX DH5α-E Competent Cells (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... PCR products were then purified with a PCR clean-up kit (Macherey-Nagel, DE), quantified with the PicoGreen assay as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... The 3C template was purified using Nucleomag PCR beads (“P-beads”, Macherey-Nagel #744100.1), eluted in 450 μl 5mM Tris-HCl (pH 8.0) ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was purified from 106 target cells using the Nucleospin RNA plus (Macherey-Nagel), and RT-PCR was performed using the QIAGEN OneStep RT-PCR kit according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... was purified by NucleoSpin® Gel and PCR clean up (Macherey-Nagel, Bethlehem, PA) after which the nucleotide sequence determined by the Sanger method (Genewiz ...
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bioRxiv - Evolutionary Biology 2021Quote: ... purified using Nucleospin® gDNA cleanup kit from Macherey-Nagel (Hudlow et al., 2011) following the supplier’s instructions with a two-fold elution step and finally quantified with Qubit® 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Transcripts were purified using the RNA Nucleospin Clean up kit (Macherey-Nagel, Bethlehem, PA). The transcribed NeuroD1 was injected at 1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The products were then purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel). We then performed 2×250 bp paired-end MiSeq sequencing (Illumina).
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bioRxiv - Genetics 2019Quote: ... Products were then purified and size-selected using NucleoMag beads (Macherey-Nagel, Düren, Germany) for two rounds of purification/size selection according to the supplied protocol at a ratio of bead solution 1.3 times the reaction volume and eluted in 30 µl EBT ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR reactions were purified with NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL). Purified reactions were PCR-amplified with primers Illumina_indX_F and Illumina_indX_R (where XXXXXXXX is index ...
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bioRxiv - Synthetic Biology 2019Quote: ... The amplified fragment was purified with NucleoSpin Gel and PCR clean-up (Macherey-Nagel) and ligated with T4 DNA Ligase (Thermo-Fisher) ...
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bioRxiv - Genetics 2019Quote: ... RNAs were purified using the NucleoSpin® RNA Clean-up XS kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... PCR products were purified using Nucleospin Gel and PCR Clean-up kits (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... All plasmids were purified using NucleoBond Xtra purification kit (MACHEREY-NAGEL Inc., Bethlehem, PA).
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bioRxiv - Microbiology 2020Quote: ... Purified rbcL amplicons (NucleoSpin® gel and PCR clean-up kit, Macherey-Nagel, France) were either Sanger-sequenced directly or cloned into pGEM-T easy vector plasmids using competent Escherichia coli JM109 cells (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel), and cloned into the LentiORF pLEX-MCS Vector (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The Psyn ThyX recombinant protein was purified on Protino Ni-TED column (Macherey-Nagel) as previously described49 ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR amplicons were purified on NucleoSpin® Gel and PCR Clean- up (Macherey-Nagel). Template DNAs of interest (500 ng each ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were gel-purified (NucleoSpin gel and PCR clean-up kit, Macherey-Nagel) and cloned into pCR-Blunt II-TOPO vectors using the Zero Blunt TOPO PCR Cloning Kit (Thermo) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using Nucleospin Gel and PCR Clean-up kits (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using the Nucleospin Gel and PCR Clean up kit (Macherey-Nagel) by following the manufacturer’s instructions with slight modifications ...
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bioRxiv - Microbiology 2022Quote: ... overlap PCR products were purified with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to the manufacturer’s instructions and DNA concentration was quantified by using NanoDrop spectrophotometer (A 260 nm).
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bioRxiv - Biochemistry 2020Quote: ... Bip-AMP was purified with Protino™ Ni-NTA Agarose (Macherey-Nagel, Düren, Germany) in previously listed buffers according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... RNA was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Germ line transmission was identified by mating sexually mature adult fish to mitfaw2/w2 fish and ...
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bioRxiv - Neuroscience 2021Quote: ... were purified using a Nucleospin Gel and PCR Clean-up kit (#740609, Macherey-Nagel) and subjected to DNA sequencing to verify if off-target activity had occurred.
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bioRxiv - Plant Biology 2021Quote: ... DNA was purified with the NucleoSpin PCR Cleanup kit (#740609; Macherey-Nagel, Düren, Germany) with buffer NTB (#740595.150 ...
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bioRxiv - Neuroscience 2021Quote: ... Transcripts were purified using the RNA Nucleospin Clean up kit (Macherey-Nagel, Bethlehem, PA). FF and reporter RLuc constructs were co-injected at 50 ng/µL ...
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bioRxiv - Synthetic Biology 2019Quote: ... digested with KpnI and purified again with PCR Clean-up kit (Macherey-Nagel, Germany). The p416-loxP-KmR-TEFmut6-yECitrine plasmid was linearized with KpnI and dephosphorylated with Antarctic Phosphatase (New England Biolabs ...
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bioRxiv - Physiology 2019Quote: ... the plasmid was purified using the NucleoSpin® Plasmid kit (Macherey-Nagel, Milan, Italy) from 4mL of culture ...
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bioRxiv - Physiology 2019Quote: ... purified with the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, Italy) and subsequently sequenced.
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bioRxiv - Cell Biology 2020Quote: ... RNA transcript was further purified with the NuceloSpin RNA Mini kit (MACHEREY-NAGEL, Germany) and quantified with spectrophotometer at 260 nm ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was purified by column capture (Macherey-Nagel Nucleospin mini kit) and sequenced (Eurofins Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel). Purified proteins were used for in vitro ubiquitination assays ...
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bioRxiv - Pathology 2021Quote: Amplicons were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and submitted to cycle-sequencing reaction by BigDye Terminator v.3.1 Cycle Sequencing kit (Ther-mo Fisher) ...
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bioRxiv - Synthetic Biology 2020Quote: ... When required DNA was purified using the NucleoSpin Extract II (Macherey-Nagel, Düren, Germany). The oligonucleotides used in this work are indicated in Supplementary Table S2 in the Supplementary Material ...
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bioRxiv - Biochemistry 2020Quote: ... The crude transcript was initially purified with NucleoSpin RNA Clean-up XS (Macherey-Nagel), followed by a second purification step using RP HPLC ...
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bioRxiv - Genomics 2021Quote: DNA was purified from collected cells using the Nucleospin Tissue XS kit (Macherey-Nagel) according to the manufacturer instructions and genotyped by nested PCR using primers described in Suppl ...
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bioRxiv - Immunology 2020Quote: ... The transformed cells were grown and the plasmid was purified by midiprep (Macherey-Nagel). The purified plasmid DNA (both heavy and light chain ...
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bioRxiv - Developmental Biology 2021Quote: ... Plasmids were purified from overnight cultures using the NucleoSpin Plasmid Miniprep Kit (Macherey-Nagel; CAT #740588 cloned gene sequences were sequenced by Sanger sequencing.
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bioRxiv - Molecular Biology 2021Quote: RNA was purified from cells using the NucleoSpin RNA Mini Kit (Macherey-Nagel. 740955.50). 1ug of RNA was reverse transcribed using the iScript gDNA Clear cDNA Synthesis Kit (BioRad 1725034 ...
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bioRxiv - Immunology 2021Quote: ... it was purified and concentrated using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel). The concentration of mRNA was measured with the QubitTM RNA BR Assay Kit and then stored at - 80⁰C in MilliQ water until use.
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bioRxiv - Microbiology 2020Quote: ... Protein was purified by SPE with a Chromabond SPE-C4 cartridge (Macherey-Nagel, Germany). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... pKP-M1144 and pKA2Q were purified using a commercial mini-prep kit (Macherey-Nagel) and transformed into calcium chloride competent MG1655 cells ...
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bioRxiv - Biochemistry 2022Quote: Plasmids and polymerase chain reaction (PCR) products were purified using kits from Macherey-Nagel. PCR fragments were amplified using Q5 High-Fidelity 2x Master Mix (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The amplicons were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... MA mRNA was purified using NucleoSpin RNA clean up kit (Macherey-Nagel, Bethlehem, PA), and passed through a Millipore Ultrafree 0.22 μm centrifugal filter (MilliporeSigma ...
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bioRxiv - Molecular Biology 2022Quote: Cellular RNA for cDNA synthesis was purified with the NucleoSpin RNA Kit (MACHEREY-NAGEL) according to manufacturer’s instructions ...