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Citations for Tonbo Biosciences :
151 - 195 of 195 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... One million cells per sample were stained with Ghost Dye-BV510 (Tonbo Biosciences, San Diego, CA) and anti-mouse CD16/CD32 (FC blocked ...
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bioRxiv - Cell Biology 2021Quote: ... cells were labelled with 5 µM carboxyfluorescein succinimidyl ester for 8 min at (CFSE; Tonbo Biosciences) for 8 min at 37°C and seeded at progressively reduced density (no less than 3000 cells/cm2) ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions from organs and tumors were incubated with Live/Dead fixable stain (Tonbo Biosciences) and Fc Block (CD16/CD32 ...
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bioRxiv - Immunology 2022Quote: ... Cells were blocked with anti-mouse CD16/CD32 (clone: 2.4G2) (Tonbo Biosciences, San Diego, CA, USA) for 5 min on ice and washed ...
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bioRxiv - Immunology 2022Quote: Cells were stained in blocking solution with a Live/Dead marker (Ghost Dye Violet510, Tonbo Biosciences) for 30 minutes at 4ºC ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained for viability using Ghost Dye Violet 510 (Tonbo Biosciences, Cat: 13-0870-T100) for 5 min on ice and washed with FACS buffer (PBS with 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were stained with a Fc receptor block (Fc Shield, Tonbo Biosciences, Cat. No. 70-0161), a rabbit antibody against the MHV68 ORF4 protein (vRCA ...
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bioRxiv - Immunology 2023Quote: ... Dead cells were excluded by a fixable viability dye (Ghost Red 710, Tonbo Biosciences, 1:6400). The following antibodies were used for flow cytometry:
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bioRxiv - Immunology 2021Quote: Single-cell suspensions were re-suspended in PBS and stained with Ghost Dye Violet 510 (Tonbo Biosciences) for dead-cell exclusion in the presence of anti-CD16/32 (Tonbo Biosciences ...
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bioRxiv - Immunology 2022Quote: ... The purified CD4 T cells or unpurified splenocytes were labelled with 1μM of CFSE (Tonbo Biosciences, CA), by incubating in a water bath at 37°C for 10 minutes ...
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bioRxiv - Immunology 2021Quote: ... myeloid DCs were quantified by gating for CD11+ cells (APC, clone 3,9 TONBO bioscience 20-0116-T100) and pDCs were quantified by gating for CD303+ cells (PE-Cy7 ...
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bioRxiv - Microbiology 2023Quote: Mouse whole blood and bone marrow samples was lysed using red blood cell lysis buffer (Tonbo biosciences) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were then stained using live/dead dye (1:1000, Ghost Dye Red 780, TONBO biosciences for myeloid ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent antibodies ...
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bioRxiv - Immunology 2023Quote: Cells were then incubated with Fc block (anti-CD16/32, 10 µg/ml, Tonbo Biosciences, San Diego, CA) for 15-minute on ice ...
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bioRxiv - Immunology 2023Quote: Freshly thawed UCBMCs (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Immunology 2024Quote: The analysis of NK cell subpopulations was performed using a Ghost Dye Red 780 viability dye (TONBO Biosciences), anti-CD56 ...
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bioRxiv - Cell Biology 2021Quote: HLAC cells were stimulated for 3 days with plates coated with 10 µg/mL anti-CD3 (UCHT1, Tonbo Biosciences) and 10 µg/mL anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for viability dye LiveDead eFlour 780 stain (eBioscience) or Ghost Dye™ Red 780 (Tonbo Biosciences) for 30 minutes ...
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bioRxiv - Immunology 2020Quote: Single cell suspensions were stained for surface antigens and a live/dead marker (Ghost Dye Violet 510, Tonbo Biosciences) in FACS buffer (PBS with 2% fetal bovine serum ...
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bioRxiv - Immunology 2020Quote: After generating a single cell suspension and staining for cpLECs with Ghost UV450 (Tonbo Biosciences, Catalog #: 13-0868-T500), CD31 Alexa 647 (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed in PBS and stained in Ghost Dye Violet 510 (Tonbo Biosciences, Cat. No.: 13-0870-T100) for 10 minutes on ice ...
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bioRxiv - Immunology 2023Quote: Freshly thawed villous leukocytes (1-2×106 cells) were stained with Ghost Violet 540 (Tonbo Biosciences, San Diego, CA) for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX ...
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bioRxiv - Neuroscience 2024Quote: Cells were then incubated for 10min at 4C in Fc Block (Tonbo Biosciences cat # 70-0161-U500, 1:2000) then washed with FACS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... A cell pellet formed and was resuspended in 2 mL of 1× lysis buffer (Tonbo Biosciences, San Diego, CA) and incubated at room temperature for 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained for viability with either GhostRed 780 (Catalog # 50-105-2988, Tonbo Biosciences, San Diego, CA) or Live-Dead Blue (Catalog # L23105 ...
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bioRxiv - Immunology 2021Quote: ... These cells were immediately stimulated with plate bounded anti-CD3 (UCHT1, coated overnight with 10μg/ml αCD3 (UCHT1, Tonbo Biosciences)) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... The marrow was then filtered through a 70µm filter and red blood cells lysed with two rounds of 1X RBC lysis buffer (Tonbo Biosciences). Cells were washed in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Lungs were plated at 1 × 106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with 1x Flow Cytometry Perm Buffer prepared as per manufacturer’s instructions (TNB-0607-KIT; Tonbo Biosciences) before staining with intracellular marker antibodies for 30 mins at 4°C in the dark (Table S2).
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs and target cells from the 24 or 72 hour co-cultures were determined by forward/side scatter and dead cells were removed using Ghost Dye Red 780 (Tonbo biosciences). T cell activation markers were stained with anti-human CD4 (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... These cells were immediately stimulated on anti-CD3-coated plates (coated overnight with 10 μg/ml anti-CD3 from Tonbo Biosciences) in the presence of 5 μg/ml soluble anti-CD28 (CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... OT-I splenocytes were stimulated with 1 nM SIINFEKL (OT-I CD8+ T cells) or in wells coated with anti-CD3 (10 μg/ml; clone: 145-2C11; Tonbo Biosciences) and anti-CD28 (2 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... About 500,000 cells were stained as follows: 30 minute incubation with 1:1000 Ghost Dye Violet 510 per manufacturer’s protocol (Tonbo Biosciences, CA), 10 minute incubation with 1:100 anti-CD16/CD32 (Tonbo Biosciences ...
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bioRxiv - Immunology 2024Quote: BMDCs and MLN cells were stained with the following Abs to identify DCs: anti-CD11c-PE/Cyanine 7 (clone; N418, TONBO Biosciences) and anti-I-A/I-E-PerCP (clone ...
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bioRxiv - Immunology 2021Quote: ... single-cell suspension were first stained for viability with LiveDead eFlour 780 stain (eBioscience) or Ghost Dye™ Red 780 (Tonbo Biosciences) for 30 minutes and then stained with antibodies and tetramers diluted in Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... followed by osmotic lysis of red blood cells (RBC) by suspension in 1mL 1X RBC Lysis Buffer (Tonbo Biosciences #TNB-4300-L100) for 3 minutes ...
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bioRxiv - Immunology 2023Quote: ... Purified naïve CD4+ T cells were stimulated with 2.5 μg/ml of anti-CD3e (Tonbo Biosciences Cat# 70-0031 (also OWL-A11492), RRID:AB_2621472 ...
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bioRxiv - Immunology 2023Quote: ... 500 μL single-cell suspensions were incubated at 37 °C for 6 h with 5% CO2 in 48-well plates in the presence of Cell Stimulation Cocktail (Tonbo Biosciences, #TNB-4975). Stimulated cells were stained with surface markers as described above ...
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bioRxiv - Microbiology 2019Quote: ... CD4+ T cells were activated for 3 days with 10 ug/mL of plate-bound anti-CD3 (Tonbo Biosciences, clone UCHT1; #70-0038-U100) and 5 ug/mL of diffused anti-CD28 (Tonbo Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... Jurkat/NL or JLTRG/NL cells were washed twice with phosphate buffered salts (PBS) and stained with Ghost Dye Red 780 (TONBO Biosciences, San Diego, CA) for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Cells were surface stained in an additional 50 uL FACS containing 1 µL Ghost Dye Violet 510 Viability Dye (Tonbo Biosciences 13-0870-T100) and a cocktail of fluorescent anti-human antibodies ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained using a dilution of 1:400 of anti-mouse CD16/CD32 (Fc Shield, #70-0161-U100; TONBO biosciences, San Diego, USA) in cRPMI for 15 minutes ...
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bioRxiv - Immunology 2022Quote: Cells were stained in PBS for 20 minutes at 4ºC with a Live/Dead marker (Ghost Dye Violet510, Tonbo Biosciences, Catalog No. 13-0870-T100). Surface antibodies were added in blocking solution for 30 minutes at 4ºC ...
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bioRxiv - Immunology 2022Quote: ... Collected lavage was spun down and cells were blocked with CD16/32 prior being stained with Ly6G PE-Cy7 (Tonbo Biosciences, 60-1276-U100, clone 1A8, 1:200) and F4/80 APC (BioLegend ...