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Citations for Tonbo Biosciences :
51 - 100 of 195 citations for B Cell Activating Factor BAFF TNFSF13B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Dead cells were excluded using Ghost DyeTM Violet 450 cell viability dye from Tonbo Biosciences. For intracellular staining of active caspase-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... NK-92 cells were collected and restimulated with cell stimulation cocktail (#TNB-4975-UL100, Tonbo Biosciences) for 4 h and intracellular staining was performed ...
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bioRxiv - Microbiology 2020Quote: ... Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2, Tonbo Biosciences). Cells were stimulated for 72 h at 37°C in a 5%-CO2 atmosphere prior to electroporation ...
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bioRxiv - Immunology 2023Quote: ... IL-10 ELISA matched antibodies were obtained from Tonbo Biosciences (San Diego, CA, USA) and the ELISA was carried out according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... The following antibodies against mouse proteins were used: anti-CD16/CD32 (2.4G2, TONBO Bioscience), anti-CD45 (30-F11 ...
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bioRxiv - Microbiology 2021Quote: ... while cell stimulation cocktail (Tonbo Biosciences) or medium alone conditions were used as positive and negative controls respectively ...
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bioRxiv - Immunology 2019Quote: ... frozen cells were thawed and stained for live cells using Ghost-Dye 710 (Tonbo Biosciences, CA, USA). 50,000 lived cells were FACS sorted and washed once with cold PBS ...
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bioRxiv - Immunology 2021Quote: ... Splenic cells underwent two rounds of red blood cell lysing using 1X RBC lysis buffer (Tonbo Biosciences). Cells were washed in PBS ...
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bioRxiv - Immunology 2020Quote: ... and the red blood cells were removed by inclubating the cells with RBC lysis buffer (Tonbo Biosciences). Atfer that ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... BM cell suspensions were lysed in 1 mL of red blood cell lysis buffer (Tonbo Biosciences, San Diego, CA), washed with 1X phosphate-buffered saline (PBS) ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse CD16/32 antibody (2.4G2) used for Fc receptor blocking was obtained from Tonbo Biosciences. Unconjugated mouse and rat IgG antibodies were purchase from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Immunology 2020Quote: ... and the following antibodies and dyes were used: Ghost UV450 (Tonbo Biosciences, Catalog #: 13-0868-T500) to visualize live/dead cells ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (Ly6eΔCD4: 1:800 anti-CD4-PECy5 [Tonbo Biosciences, CA]/1:400 anti-CD3ε-FITC [Tonbo Biosciences ...
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bioRxiv - Immunology 2020Quote: ... Single cell suspensions were stained with 1:1000 Ghost dye violet 510 dead cell stain for 405nm excitation (Tonbo biosciences) in PBS for 30 min followed by a wash with 2% NCS HBSS ...
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bioRxiv - Cancer Biology 2021Quote: ... Anti-mouse CD8α-APC antibody (0.7 μL; cat. #20-1886, Clone 2.43, Tonbo Biosciences, San Diego, CA) was added then incubated for 10 min at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... anti-CD3 (17A2) and anti-CD45R/B220 (RA3) antibodies were obtained from Tonbo Biosciences (San Diego, CA); anti-CD45 (30-F11 ...
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bioRxiv - Bioengineering 2024Quote: ... This was followed by an HRP secondary antibody (Tonbo Biosciences, San Diego, CA, Cat # 72-8104-M001). Chromogen was developed with a DAB HRP substrate (Vector Labs ...
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bioRxiv - Immunology 2020Quote: ... cells suspensions were stimulated for 4 hours ex vivo using a commercial cell stimulation cocktail (Tonbo Biosciences, catalog no. TNB-4975) prior to staining for flow cytometry as above ...
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bioRxiv - Developmental Biology 2022Quote: Lung cells were plated at 1×106 cells per well and stained with Fc block (CD16/32, 1:100, Tonbo Biosciences) for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were washed thoroughly and stained for dead cell exclusion (Ghost Violet 510, 1:1000 dilution Tonbo Biosciences, San Diego, CA) for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The myeloid panel contained GhostDye (1:1000) and antibodies directed against CD45 (Tonbo Biosciences #50-0451; 1:250), CD11b (Tonbo Biosciences #75-0112 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in the study are as follows: APC or FITC anti-mouse CD45.1 (clone: A20, Tonbo Biosciences), Alexa Fluor 700 anti-mouse CD45 (Clone ...
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bioRxiv - Immunology 2023Quote: ... Additional antibodies used for this experiment included anti-IFNψ-APC (Tonbo Biosciences Cat# 20-7311 (also OWL-A01038), RRID:AB_2621616) ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were stained with Ghost Dye 450 (TONBO biosciences) prior to 4% PFA fixation to stain dead cells ...
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bioRxiv - Immunology 2021Quote: The following antibodies were used for conjugation assay: anti-human CD3-FITC (clone OKT3; Tonbo Biosciences# 35-0037-T100), anti-Flag-APC (clone L5 ...
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bioRxiv - Immunology 2024Quote: ... Anti-mouse CD16/CD32 Fc Block antibody purchased at a concentration of 2mg/mL (Tonbo Biosciences 40-0161-M001) was added at 0.5 uL per sample (in 100uL total volume per sample of block antibody diluted in PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with Ghost Dye viability dye (TONBO biosciences) for 30 minutes at 4C per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with GHOST viability dye from Tonbo Biosciences and flow cytometry was performed using anti-IL18R1 [H44] and anti-CD70 [113-16] antibodies ...
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bioRxiv - Microbiology 2020Quote: ... fixed cells were treated with anti-CD16/CD32 (Tonbo Biosciences) and then stained with antibodies that recognize surface lineage markers and analyzed by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... Cell viability was determined with Ghost Dye 780 (Tonbo Biosciences). Enumeration of cells was done using PKH26 reference microbeads (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with Ghost Dye Violet 510 (Tonbo Biosciences) for 10 min at RT in PBS+1mM EDTA ...
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bioRxiv - Immunology 2022Quote: ... we stained cells with Ghost Dye Violet 510 (Tonbo Biosciences), anti-mouse CD16/32 (clone 2.4G2 ...
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bioRxiv - Immunology 2024Quote: ... Cell viability was examined by Fixable viability dye (Tonbo Bioscience) or 7-AAD (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2020Quote: ... unstimulated and cytokine-stimulated 2D cultures of RAW264.7 macrophages were stained according to supplier instructions with both PE-conjugated CD86 antibody (Tonbo Biosciences) and FITC-conjugated CD206 antibody (BioRad ...
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bioRxiv - Immunology 2021Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BV570/BV605 ...
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bioRxiv - Immunology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD3e (PE-Cy5/AF532 ...
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bioRxiv - Microbiology 2023Quote: ... Antibodies used in various combinations (depending on experiment) are as follows: Ghost Viability Dye (v510, Tonbo Biosciences, 1:1000 dilution), CD45 (BUV395 ...
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bioRxiv - Immunology 2021Quote: ... or a Live/Dead fixable cell stain (Ghost 780 Tonbo Biosciences) was used to exclude dead cells in all experiments ...
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bioRxiv - Immunology 2022Quote: ... Red blood cells were lysed using 1X lysis buffer (Tonbo Biosciences). Cells were then counted and 1×107 splenocytes were transferred intraperitonially into Thy1.1 congenic mice ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined using Ghost Dye Red 780 (Tonbo Biosciences). Flow cytometry was performed on a LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with Ghost Live Dead (Tonbo Biosciences; 13-0870), Annexin V (Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Red blood cells were lysed with RBC lysis buffer (Tonbo Biosciences). Epithelial cells ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were stained with Ghost Dye Red Viability Dye (Tonbo Biosciences) to calculate dead cells ...
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bioRxiv - Immunology 2024Quote: ... cell viability was determined using Ghost Dye Violet 510 (TONBO biosciences). Absolute cell quantification was performed using CountBright Absolute Counting Beads (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... red blood cells were lysed using RBC lysis buffer (Tonbo Biosciences), cells were blocked with anti-CD16/CD32 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated for 20 minutes at RT with mouse PE conjugated 5-OP-RU MR1-tetramer (NIH Tetramer core) and the following antibodies: anti-CD4-FITC/APC-Fire810 (clone GK1.5, Tonbo Biosciences/Biolegend), anti-CD8α-AF700 (clone 53-6.7 ...
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bioRxiv - Microbiology 2020Quote: ... bulk CD4+ T cells were immediately plated on anti-CD3-coated plates [coated for 12 hours at 4°C with 20 µg/ml anti-CD3 antibody (UCHT1, Tonbo Biosciences)] in the presence of 5 µg/ml soluble anti-CD28 antibody (CD28.2 ...
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bioRxiv - Immunology 2021Quote: ... After the incubation a cocktail of fluorescently-labeled surface antibodies (2 µL of each: Ghost Dye Violet 510 Viability Dye, Tonbo Biosciences 13-0870-T100 ...
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bioRxiv - Microbiology 2023Quote: ... and 20 minute incubation with primary antibody cocktail (Ly6eΔCD4: 1:800 anti-CD4-PECy5 [Tonbo Biosciences, CA]/1:400 anti-CD3ε-FITC [Tonbo Biosciences, CA] ...
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bioRxiv - Immunology 2022Quote: ... The cells were then incubated in 1X Perm/Wash buffer (Tonbo Biosciences) for 30 minutes to permeabilize the cell membrane ...