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Citations for Applied Biological Materials :
51 - 100 of 100 citations for Human PDGF alpha receptor PDGFRA qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: T0034 immortalized human skeletal muscle cell line was purchased from ABM. Cell were maintained in Prigrow III medium (ABM) ...
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bioRxiv - Immunology 2022Quote: ... and immortalized human cardiac fibroblasts (iHCF, Applied Biological Materials Inc., T0446) were grown in FibroGRO LS medium (Millipore ...
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bioRxiv - Cell Biology 2023Quote: The immortalized (SV40) human microglial cell line was acquired from ABM. Human microglial cells were cultured in cell culture flasks coated with 50 μg/ml rat tail collagen I (Gibco ...
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bioRxiv - Microbiology 2024Quote: Lund Human Mesencephalic Cells (LUHMES) were obtained from Applied Biological Materials Inc ...
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bioRxiv - Genomics 2024Quote: Pools of immortalized human cardiac fibroblasts (iHCF) (Applied Biological Materials, Inc.) were co-transduced with lentiviruses containing guides against the same enhancer locus and Cas9-Blasticidin lentivirus (Transomic ...
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bioRxiv - Immunology 2021Quote: ... The lentivirus titer was measured using a Lentivirus qPCR Titer kit (Applied Biological Materials, Richmond, BC, Canada). For lentiviral transduction ...
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bioRxiv - Molecular Biology 2022Quote: ... The lentivirus titer was measured using a Lentivirus qPCR Titer kit (Applied Biological Materials, Richmond, BC, Canada).
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bioRxiv - Cancer Biology 2022Quote: ... The lentivirus titer determined using the qPCR lentivirus titer kit was ~108 IU/mL (Applied Biological Materials).
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bioRxiv - Immunology 2023Quote: ... The lentivirus titer determined using the qPCR lentivirus titer kit was >109 IU/mL (Applied Biological Materials).
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bioRxiv - Cancer Biology 2023Quote: ... Concentrations of viral particles were verified via qPCR using a lentiviral titration kit from Applied Biological Materials on QuantStudio 3 from Thermo Fisher.
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bioRxiv - Neuroscience 2022Quote: Human immortalized microglial cell line SV-40 (T0251, Applied Biological Materials Inc.) originates from the Tardieu lab(30 ...
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bioRxiv - Molecular Biology 2024Quote: ... The lentiviral vectors were then titrated with qPCR Lentivirus Titration (Titer) Kit (ABM®, LV900, Richmond, BC, Canada). The absence of mycoplasma was confirmed using MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... 500 ng of DNase-treated RNA was converted into cDNA using Oligo-dT primers in a reaction volume of 10 μl using the OneScript Plus cDNA synthesis kit (Catalog no. G236; ABM). The cDNA was amplified using primer pairs specific to Tat ...
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bioRxiv - Bioengineering 2020Quote: ... RNA purification and reverse transcription were performed as previously described.[27] qPCR master-mix (Applied Biological Materials Inc., Canada) was used for PCR amplification ...
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bioRxiv - Microbiology 2021Quote: The titer of the virus preparations was determined by using the qPCR lentivirus titration kit (Applied Biological Materials, USA) as per the manufacturer-recommended protocol ...
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bioRxiv - Microbiology 2024Quote: ... The transduction units of the produced lentiviruses were titrated using qPCR Lentivirus Titer Kit (#LV900, ABM, Richmond, BC, Canada).
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bioRxiv - Cancer Biology 2020Quote: ... The human microglia (hMG) cell line was purchased from Applied Biological Materials (ABM). Mouse glioblastoma cell GL261 was generously provided by Dr ...
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bioRxiv - Immunology 2024Quote: Immortalized human fetal astrocytes-SV40 (IMhu-A) (Applied Biological Materials, Cat. No. T0280) cells were grown in Roswell Park Memorial Institute (RPMI)-HEPES medium (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: Human lung fibroblasts (hTERT T1015 cell line purchased from Applied Biological Materials Inc.) were used before passage 5 for all experiments ...
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bioRxiv - Neuroscience 2020Quote: ... Virus titre was quantified using the qPCR Lentivirus Titration Kit according to manufacturer’s directions (Applied Biological Materials, British Columbia, Canada).
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bioRxiv - Cancer Biology 2020Quote: ... Gene expression to validate RNA-seq analysis was quantified by qRT-PCR using BrightGreen 2X qPCR MasterMix (ABM, Vancouver, Canada) on a QuantStudio 3 real-time PCR system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... Stocks of Lenti-Control and Lenti-NICD3 were titrated by qPCR (catalog number LV900, ABM® good, Richmond, BC, Canada) and their infectivity confirmed by flow cytometry via quantification of GFP+ cells ...
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bioRxiv - Genomics 2024Quote: ... SV40-immortalized human microglia cells (Cat.No: T0251, Applied Biological Materials Inc, Richmond, QC, Canada), was cultured in Prigrow III medium with 10% (vol/vol ...
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bioRxiv - Physiology 2023Quote: Human VSMCs and mouse VSMCs were purchased from Applied Biological Materials (T0515, Richmond, Canada) and American Type Culture Collection (ATCC) ...
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bioRxiv - Microbiology 2022Quote: ... All experiments were performed in triplicates in total reaction volumes of 15 μL using BrightGreen 2X qPCR MasterMix-No Dye (ABM Inc.). A housekeeping gene was used to normalize gene expression ...
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bioRxiv - Cancer Biology 2021Quote: HKDC1 sgRNA CRISPR All-in-One Lentivirus (Human) (Cat No. 234181110603) was purchased from ABM and HepG2 cells (70% confluent ...
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bioRxiv - Immunology 2024Quote: ... The immortalized human microglia-SV40 (IMhu-M) cell line (Applied Biological Materials, Cat. No. T0251), HEK293(T ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus particles expressing human α-syn under cytomegalovirus (CMV) promoter (Applied Biological Materials, Inc, Canada) was used to re-express α-syn in SK-MEL-28 SNCA-KO cells as described previously27
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus particles expressing human α-syn under cytomegalovirus (CMV) promoter (Applied Biological Materials, Inc, Canada) was used to re-express α-syn in SK-MEL-28 KO cells as described previously30 ...
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bioRxiv - Microbiology 2022Quote: ... 2μl of pre-amplified cDNA or NRT control reaction was used as template for multiplexed real-time PCR reactions using TaqProbe 5x qPCR MasterMix-Multiplex (ABM MasterMix-5PM), 5% DMSO ...
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bioRxiv - Biophysics 2021Quote: ... The products obtained from the X and C region pull-downs were PCR-amplified using the same primers used for their production (see above) and amplicons visualized with agarose gels and SafeView→ (Applied Biological Materials Inc., Vancouver) staining under UV light.
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bioRxiv - Cell Biology 2024Quote: ... The CRISPR/Cas9 system specifically targeting human ATG7 (K0142505) was purchased from Applied Biological Materials (ABM) Inc ...
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bioRxiv - Biochemistry 2024Quote: ... and human CDC14A siRNA and scrambled siRNA (i504211) were from Applied Biological Materials (Richmond, BC, Canada). FAK-14 inhibitor was from Tocris Bioscience (Bristol ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... The cell suspension was then diluted in StemFlex with ROCK inhibitor to a concentration of 1e6 cell/mL and mixed with 50uL aliquots of both tetO-ALN-PuroR and pUBIQ-rtTA viruses tittered at an estimated 1 × 107 IU/mL using a qPCR Lentivirus Titration Kit (Applied Biological Materials, #LV900). hiPSCs were plated on Matrigel-coated plates and incubated at 37°C with virus overnight ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR amplification of the cDNA was analyzed using Bright Green 2X qPCR Master Mix-Low ROX (Applied Biological Materials Inc, BC, Canada) and QS12K Flex system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant adenovirus expressing human ACE2 (Ad5-hACE2) and control adenovirus (Ad5-Ctrl) were purchased from ABM (Vancouver, Canada).
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bioRxiv - Cell Biology 2020Quote: ... lentiviral sgRNA targeting human LDLR (Cat# 264181110204) and non-viral plasmids containing sgRNA targeting individual candidate genes were purchased from ABM Goods ...
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bioRxiv - Microbiology 2020Quote: The recombinant adenovirus 5 expressing human ACE2 (Ad5-hACE2) and control adenovirus (Ad5-Ctrl) were purchased from ABM (Vancouver, Canada) or generated in the laboratory ...
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bioRxiv - Genetics 2022Quote: The SMN1 AAV Vector (Human; hSyn; Luc) (#444551011210) containing the functional, synthetic SMN1 gene (GenBank accession code, NM_000344) was obtained from Applied Biological Materials Inc ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: For all experiments human embryonic kidney (HEK) 293SL cells84 were used and regularly tested for mycoplasma contamination (PCR Mycoplasma Detection kit, ABM, Canada). HEK293SL cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Immortalized human epithelial endometriotic cells (12Z cell line,[98] Cellosaurus accession no. CVCL0Q73, female sex) were purchased from Applied Biological Materials, Canada (Ref T0764) ...
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bioRxiv - Cancer Biology 2024Quote: ... Human SV40 immortalized microglial cells (HMSV40) were cultured in PriCoat T25 flasks in Prigrow III Medium (Applied Biological Materials, Richmond, Canada) supplemented with 10% FBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... ready-to-use lentivirus particles containing three unique sgRNA targeting human Itch or viruses containing scrambled sgRNA were purchased from Applied Biological Materials Inc ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Immortalized human endometrial stromal cells (T-HESC cell line,[88] Cellosaurus accession no. CVCLC464, female sex) were purchased from Applied Biological Materials, Canada (Ref T0533) ...
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bioRxiv - Developmental Biology 2022Quote: The use of human embryos donated for this project was allowed by the Agence de la Biomédecine (ABM, approval number RE 17- 011R) in compliance with the International Society for Stem Cell Research guidelines32 ...
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bioRxiv - Cell Biology 2020Quote: Stably Cas9-expressing HepG2 cells were transduced at a multiplicity of infection (MOI) of 1.5 with lentivirus containing a sgRNA targeting human LDLR (ABM Goods, Inc. Canada, Cat# 264181110204) and a neomycin selection marker ...
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bioRxiv - Bioengineering 2022Quote: ... Adventitia-derived pericytes were isolated from non-aneurysmal human aortic specimens and immortalized to improve culture longevity using a lentiviral vector system to deliver HPV-E6/E7 (ABM, Richmond, British Columbia, Canada) using previously described methods.22,23
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bioRxiv - Cancer Biology 2023Quote: 3T3-L1 cells were stably transfected with a human POSTN lentiviral vector (pLenti-GIII-CMV-RFP-2A-Puro-POSTN, Applied Biological Materials Inc. Cat# LV268492) and control vehicle plasmid (pLenti-CMV-RFP-2A-Puro-Blank Vector ...
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bioRxiv - Cancer Biology 2023Quote: ... in a pLenti-Puro vector (pLenti-All-in-one-U6-sgRNA human Zeb1 (target1) or scramble -SFFV-Cas9 nuclease-2A-Puro) (Applied Biological Materials Inc., Richmond, Canada). The sequences of the sgRNA targeting ZEB1 are the following ...
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bioRxiv - Molecular Biology 2024Quote: ... Human RPL22 and RPL22L1 cDNAs were amplified from pLenti-GIII-CMV-RPL22-HA and pLenti-GIII-CMV-RPL22L1-HA (Applied Biological Materials, cat # LV291610, cat # LV802168) then cloned into a PB-EF1α-MCS- IRES-GFP PiggyBac expression vector (System Biosciences ...